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| 1 | New serum biomarkers for detection of HBV-induced liver cirrhosis using SELDI protein chip technology显示文摘AIM: To find new serum biomarkers for liver cirrhosis (LC) in chronic carriers of hepatitis B virus (HBV).METHODS: Surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry was used to discover biomarkers for differentiating HBV induced LC from non-cirrhotic cohorts. A training population of 25 patients with HBV-induced LC, 20 patients with HCC, and 25 closely age-matched healthy men, was studied.RESULTS: Two biomarkers with Mr 7 772 and 3 933 were detected in sera of non-cirrhotic cohorts, but not in patients with HBV-induced LC. A sensitivity of 80% for all LC patients,a specificity of 81.8% for all non-cirrhotic cohorts and a positive predictive value of 75% for the study population were obtained.CONCLUSION: These two serum biomarkers for HBV-induced LC might be used for diagnosis and assessment of disease progression. | Xiao-DongZhu Wei-HuaZhang Cheng-LinLi YangXu Wei-JiangLiang PoTien | 2004 | World Journal of Gastroenterology2004，10，16： | 37 |
| 2 | Significant correlation between expression level of HSP gp96 and progression of hepatitis B virus induced diseases显示文摘Gp96, also known as Grp94, is a member of heatshock protein (HSP) family and binds repertoires of peptidesthereof eliciting peptide-specific T cell immune responses.It predominantly locates inside the endoplasmic reticulum(ER) with some cell surface expression in certain cancerouscells. Previous studies have shown that gp96 expressionlevel was up-regulated in tumor cells, including hepatocellularcarcinoma (HCC). However, relationship between theextent of gp96 expression and disease progression especiallyHBV-induced chronic infection, cirrhosis and hepatocellularcarcinoma, has not been addressed before. As primary HCCcan be induced and progressed from chronic hepatitis Bvirus (HBV) infection and HBV-induced cirrhosis, wedesigned an immunohistochemical experiment to test thecorrelation between gp96 expression level and HBV-induceddisease progression, from chronic HBV infection, cirrhosisto HCC. | Xiao-DongZhu Cheng-LinLi Zhen-WeiLang GeorgeFGao PoTien | 2004 | World Journal of Gastroenterology2004，10，8： | 22 |
| 3 | Enhancement of humoral immune responses to HBsAg by heat shock protein gp96 and its N-terminal fragment in mice显示文摘AIM: Most studies on the immune effect of gp96 were focused on its enhancement of CTLs. It is interesting to know whether gp96 could influence the humoral immune response, and whether the recombinant N-terminal fragment of gp96 could substitute native gp96 to stimulate the immune system.METHODS: gp96 isolated from livers of normal mice and its N-terminal fragment (amino acid 22-355) expressed in E coli were used for immunization of BALb/c mice. Eight groups of mice received one of the following regiments subcutaneously in 100 μL phosphate buffered saline (PBS)at an interval of 3 wk. Group 1: PBS only; group 2:gp96 only; group 3: N-terminal fragment only; group 4: HBsAg only; group 5: HBsAg+gp96; group 6: HBsAg+N-terminalfragment; group 7: HBsAg+incomplete Freud's adjuvant; group 8: HBsAg+N-terminal fragment (95 ℃ heated for 30 min). Serum anti-HBsAg antibody levels were assayed by ELISA. CTL responses in splenocytes were analyzed by ELISPOT after the last vaccination.RESULTS: The average titer of serum anti-HBsAg antibodyin the mice immunized with HBsAg together with gp96 or its N-terminal fragment were much higher than those immunized with HBsAg alone detected by ELISA. The cellular immune response of the mice immunized with HBsAg together with gp96 or its N-terminal fragment was not different with those immunized with HBsAg alone measured by ELISPOT assay.CONCLUSION: gp96 or its N-terminal fragment greatly improved humoral immune response induced by HBsAg, but failed to enhance the CTL response, which demonstrated the potential of using gp96 or its N-terminal fragment as a possible adjuvant to augment humoral immune response against HBV infection. | Hong-TaoLi Jia-BinYan JingLi Ming-HaiZhou Xiao-DongZhu Yu-XiaZhang PoTien | 2005 | World Journal of Gastroenterology2005，11，19： | 6 |
| 4 | Protection of the small intestinal clonogenic stem ceils from radiation-induced damage by pretreatment with interleukin 11 also incus routine survival time显示文摘 | POTIEN C S | 1996 | Stem Cells1996，14，4： | 1 |
| 5 | PD‐1 and PD‐L1 upregulation promotes CD8+ T‐cell apoptosis and postoperative recurrence in hepatocellular carcinoma patients显示文摘 | FengShi MingShi ZhenZeng Rui‐ZhaoQi Zhen‐WenLiu Ji‐YuanZhang Yong‐PingYang PoTien Fu‐ShengWang | 2010 | Int. J. Cancer2010，，： | 1 |
| 6 | Measurement of the qu- antity practical peak voltage in the radiology practice显示文摘 | Terini RA Potiens M Herdade SB | 2009 | Radiol Bras2009，42，6： | 1 |
| 7 | Identification of a putative intestinal stem cell and early lineage marker;musashi-1 显示文摘 | POTIEN C S BOOTH C TUDOR G L | 2003 | Differentiation2003，71，： | 1 |
| 8 | An Engineered PrP^sc-like Molecule from the Chimera of Mammalian Prion Protein and Yeast Ure2p Prion-inducing Domain显示文摘Production of the pathogenic prion isoform PrP^sc-like molecules is thought to be useful forunderstanding the mysterious mechanism of conformational conversion process of prion diseases andproving the 'protein-only' hypothesis. In this report, an engineered PrP^sc-like conformation was producedfrom a chimera of mammalian bovine prion protein (bPrP) and yeast Ure2p prion-inducing domain (UPrD).Compared with the normal form of bPrP, the engineered recombinant protein, termed bPrP-UPrD,spontaneously aggregated into ordered fibrils under physiological condition, displaying amyloid-likecharacteristics, such as fibrillar morphology, birefringence upon binding to Congo red and increasedfluorescence intensity with Thioflavine T. Limited resistance to protease K digestion and CD spectroscopyexperiments suggested that the structure of bPrP-UPrD had been changed, and adopted a new, high contentB-sheet conformation during the fibrils formation. Moreover, bPrP-UPrD amyloid fibrils could recruit moresoluble forms into the aggregates. Therefore, the engineered molecules could mimic significant behaviors of PrP^se and will be helpful for further understanding the mechanism of conformational conversion process. | Shao-ManYIN Man-SunSY PoTIEN | 2004 | Acta Biochimica et Biophysica Sinica2004，36，2： | 0 |
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