|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Application of Principal Component Analysis(PCA)to the Evaluation and Screening of Multiactivity Fungi显示文摘Continued innovation in screening methodologies remains important for the discovery of high-quality multiactive fungi,which have been of great significance to the development of new drugs.Mangrove-derived fungi,which are well recognized as prolific sources of natural products,are worth sustained attention and further study.In this study,118 fungi,which mainly included Aspergillus spp.(34.62%)and Penicillium spp.(15.38%),were isolated from the mangrove ecosystem of the Maowei Sea,and 83.1%of the cultured fungi showed at least one bioactivity in four antibacterial and three antioxidant assays.To accurately evaluate the fungal bioactivities,the fungi with multiple bioactivities were successfully evaluated and screened by principal component analysis(PCA),and this analysis provided a dataset for comparing and selecting multibioactive fungi.Among the 118 mangrove-derived fungi tested in this study,Aspergillus spp.showed the best comprehensive activity.Fungi such as A.clavatonanicus,A.flavipes and A.citrinoterreus,which exhibited high comprehensive bioactivity as determined by the PCA,have great potential in the exploitation of natural products and the development of new drugs.This study demonstrated the first use of PCA as a time-saving,scientific method with a strong ability to evaluate and screen multiactive fungi,which indicated that this method can affect the discovery and development of new drugs. | YANG Zonglin SHI Yaqi LI Pinglin PAN Kanghong LI Guoqiang LI Xianguo YAO Shuo ZHANG Dahai | 2022 | Journal of Ocean University of China2022，21，3： | 4 |
| 2 | MFTZ‐1 reduces constitutive and inducible HIF‐1α accumulation and VEGF secretion independent of its topoisomerase II inhibition显示文摘 | MeiDai Ze‐HongMiao XuanRen Lin‐JiangTong NaYang TingLi Li‐PingLin Yue‐MaoShen JianDing | 2009 | Journal of Cellular and Molecular Medicine2009，，9： | 2 |
| 3 | Proliferation and differentiation of reactive nestin^+/GFAP^+ cells in an adult rat model of compression-induced spinal cord injury显示文摘BACKGROUND:Studies have demonstrated that astrocytes may possess similar properties to neural stem cells/neural precursor cells and have the potential to differentiate into neurons. OBJECTIVE:To observe neuroepithelial stem cell protein(nestin) and glial fibrillary acidic protein (GFAP) expression following spinal cord injury,and to explore whether nestin^+/GFAP^+ cells,which are detected at peak levels in gray and white matter around the ependymal region of the central canal in injured spinal cord,possess similar properties of neural stem cells. DESIGN,TIME AND SETTING:A randomized,controlled experiment.The study was performed at the Key Laboratory of Environment and Genes Related to Diseases(Xi'an Jiaotong University), Ministry of Education between January 2004 and December 2006. MATERIALS:Rabbit anti-rat nestin,β-tubulinⅢ,mouse anti-rat GFAP,galactocerebroside(GaLC) antibodies were utilized,as well as flow cytometry. METHODS:A total of 60 male,Sprague Dawley rats,aged 8 weeks,were randomly assigned to control(n = 12) and model(n = 48) groups.The spinal cord injury model was established in the model group by aneurysm clip compression,while the control animals were not treated.The gray and white matter around the ependymal region of the central canal exhibited peak expression of nestin^+/GFAP^+ cells.These cells were harvested and prepared into single cell suspension,followed by primary and passage cultures.The cells were incubated with serum-containing neural stem cell complete medium. MAIN OUTCOME MEASURES:Nestin and GFAP expression in injured spinal cord was determined using immunohistochemistry and double-labeled immunofluorescence at 1,3,5,7,14,28,and 56 days post-injury.In addition,cell proliferation and differentiation were detected using immunofluorescence cytochemistry and flow cytometry. RESULTS:Compared with the control group,the model group exhibited significantly increased nestin and GFAP expression(P<0.05),which reached peak levels between 3 and 7 days.The majority of cells in the ependymal region around the central canal were nestin^+/GFAP^- cells,while the gray and white matter around the ependymal region were full of nestin^+/GFAP^+ cells,with an astrocytic-like appearance.A large number of nestin^+/GFAP^+ cells were observed in the model group cell culture,and the cells formed clonal spheres and displayed strong nestin-positive immunofluorescence staining.Following induced differentiation,a large number of GaLC-nestin,β-tubulinⅢ-nestin,and GFAP-nestin positive cells were observed.However,no obvious changes were seen in the control group.Cells in S stage,as well as the percentage of proliferating cells,in the model group were significantly greater than in the control group(P<0.01). CONCLUSION:Spinal cord injury in the adult rat induced high expression of nestin^+/GFAP^+ in the gray and white matter around the ependymal region of the central canal.These nestin^+/GFAP^+ cells displayed the potential to self-renew and differentiate into various cells.The cells could be neural stem cells of the central nervous system. | Pinglin Yang Xijing He Haopeng Li Binshang Lan Guoyu Wang Yiheng Liu | 2009 | Neural Regeneration Research2009，4，10： | 1 |
| 4 | The step distance of first caving of hard roof显示文摘 | Zhao Jun Li Fengying Jiang Pinglin | 1991 | Ground Pressure and Strata Control1991，，3： | 1 |
| 5 | 显示文摘 | Li Pinglin Zhang Jincang Cao Guixin | 2004 | Phys Rev2004，69，22： | 1 |
| 6 | Kalihioxepanes A-G:Seven Kalihinene Diterpenoids from Marine Sponge Acanthella cavernosa Collected off the South China Sea显示文摘Comprehensive Summary Seven new kalihinene diterpenoids,kalihioxepanes A-G(1-7)bearing a rare oxepane ring,were isolated from sponge Acanthella cavernosa collected from the South China Sea.The structures and absolute configurations were elucidated by comprehensive spectroscopic analysis,single crystal X-ray diffraction,and quantum chemical calculation methods.Kalihioxepane A(1)displayed strong cytotoxicity against H69 and K562 tumor cells,while kalihioxepane B(2)showed moderate cytotoxicity against K562 cells. | Zhe Wang Xiao Han Guoping Liu Di Zhang Huiyue Hou Li Xiao Nicole J.de Voogd Xuli Tang Pinglin Li | 2022 | Chinese Journal of Chemistry2022，40，15： | 1 |
| 7 | Optimized One-Step Preparation of a Bioactive Natural Product,Guaiazulene-2,9-dione显示文摘We previously isolated a natural product, namely guaiazulene-2,9-dione showing strong antibacterial activity against Vibrio anguillarum, from a gorgonian Muriceides collaris collected in South China Sea. In this experiment, guaiazulene-2,9-dione was quantitatively synthesized with an optimized one-step bromine oxidation method using guaiazulene as the raw material. The key reaction condition including reaction time and temperature, drop rate of bromine, concentration of aqueous THF solution, respective molar ratio of guaiazulene to bromine and acetic acid, and concentration of guaiazulene in aqueous THF solution, were investigated individually at five levels each for optimization. Combined with the verification test to show the absolute yield of each optimization step, the final optimal condition was determined as: when a solution of 0.025 mmol m L-1 guaiazulene in 80% aqueous THF was treated with four volumes of bromine at a drop rate of 0.1 m L min-1 and four volumes of acetic acid at-5℃ for three hours, the yield of guaiazulene-2,9-dione was 23.72%. This was the first report concerning optimized one-step synthesis to provide a convenient method for the large preparation of guaiazulene-2,9-dione. | CHENG Canling LI Pinglin WANG Wei SHI Xuefeng ZHANG Gang ZHU Hongyan WU Rongcui TANG Xuli LI Guoqiang | 2014 | Journal of Ocean University of China2014，13，6： | 0 |
| 8 | Qualitative and quantitative relationships between affinity constants from model study and real adsorption data显示文摘Linear polymers bearing the same functional groups as their corresponding adsorbents could be used as models to study the adsorption behaviors.However,the relationships between the data from model study and real adsorption have not been fully interpreted.In this work,three adsorbent(CP1-Zn2+,CP2-Zn2+ and CP3-Zn2+) and their corresponding linear models(P1-Zn2+,P2-Zn2+ and P3-Zn2+) were synthesized.The affinity constants(Ka) between these models and target peptide DFLAE(DE5) were obtained by isothermal titration calorimetry(ITC).Adsorption capacities and adsorption affinities were carried out by static adsorption and adsorption isotherm.The qualitative and quantitative relationships between affinity constants from model study and the real adsorption data were explored.This study was significant to bridge the model study with the real adsorption. | QIAO YiTao LI PingLin ZHAO JianXin WANG Wei YUAN Zhi | 2010 | Chinese Science Bulletin2010，55，29： | 0 |
| 9 | Affinity adsorption mechanism studies of adsorbents C1-Zn(Ⅱ) for uremic middle molecular peptides containing Asp-Phe-Leu-Ala-Glu sequence显示文摘To exploit efficient adsorbents for removing middle molecular peptides containing DFLAE (DE5,a typical peptide sequence accumulated in uremic serum) sequence by hemoperfusion,we designed and synthesized three affinity adsorbents (C1-Zn2+,C2-Zn2+ and C3-Zn2+) that could have high affinity to DE5.Subsequently,we evaluated the corresponding adsorption ability of each adsorbent by static adsorption experiments and isothermal titration calorimetry (ITC).The results showed that C1-Zn2+ had the best adsorption ability to DE5-containing peptides and the adsorption capacity for DE5 was 8.52 mg/g.By changing the adsorption conditions,the adsorption mechanism was elucidated.The main driving force of the adsorption is metal-carboxyl coordination and the hydrophobic force affords the cooperative effect.It is expected that our present work can provide basic understanding for the design of adsorbents with high affinity and selectivity towards oligopeptides. | LI PingLin FU LiXue QIAO YiTao ZHAO JianXin WANG Wei&YUAN Zhi | 2011 | Science China Chemistry2011，54，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员