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| 1 | Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand显示文摘It was suggested that chronic ethanol exposure could result in testicular germ cell apoptosis, but the mechanism is still unclear. In the present study, we use a model of transgenic mice ubiquitously overexpressing human FasL to investigate whether Fas ligand plays a role in ethanol-induced testicular germ cell apoptosis. Both wild-type (WT)mice and transgenic (TG) mice were treated with acute ethanol (20% v/v) by introperitoneal injection for five times.After ethanol injection, WT mice displayed up-regulation of Fas ligand in the testes, which was shown by FITCconjugated flow cytometry and western blotting. Moreover, TG mice exhibited significantly more apoptotic germ cells than WT mice did after ethanol injection, which was demonstrated by DNA fragmentation, PI staining flow cytometry and TUNEL staining. In addition, histopathological examination revealed that degenerative changes of epithelial component of the tubules occurred in FasL overexpressing transgenic mice while testicular morphology was normal in wild-type mice after acute ethanol exposure, suggesting FasL expression determines the sensitivity of testes to ethanol in mice. In summary, we provide the direct evidences that Fas ligand mediates the apoptosis of testicular germ cells induced by acute ethanol using FasL transgenic mice. | JIAHUAHU JIEJIANG YINGHUAMA NAYANG MAOHUZHANG MINWU JIANFEI LIHEGUO | 2003 | Cell Research2003，13，5： | 16 |
| 2 | MFTZ‐1 reduces constitutive and inducible HIF‐1α accumulation and VEGF secretion independent of its topoisomerase II inhibition显示文摘 | MeiDai Ze‐HongMiao XuanRen Lin‐JiangTong NaYang TingLi Li‐PingLin Yue‐MaoShen JianDing | 2009 | Journal of Cellular and Molecular Medicine2009，，9： | 2 |
| 3 | Identification of glutamate transporters and receptors in mouse testis显示文摘AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transport- ers in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 ± 0.21) pmol/min per mg protein and Km value of glutamate uptake was (11.0±1.6) μmol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2B, GluR6 and KA2) were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis. | Jia-huaHU NaYANG Ying-huaMA JieJIANG Jin-fuZHANG JianFEI Li-heGUO | 2004 | Acta Pharmacologica Sinica2004，25，3： | 1 |
| 4 | Impaired reproduction in transgenic mice overexpressing γ-aminobutyric acid transporter I (GAT1)显示文摘It is well documented that γ-aminobutyric acid (GABA) system existed in reproductive organs. Recent researches showed that GABAA and GABAB receptors were present in testis and sperm,and might mediate the acrosome reaction induced by GABA and progesterone. GABA transporter I (GAT1) also existed in testis and sperm,but its physiological function was unknown. In the present study,we used GAT1 overexpressing mice to explore GAT1 function in male reproductive system. We found that the expression level of GAT1 continuously increased in wild-type mouse testis from 1 month to 2 months after birth. GAT1 overexpression in mouse affected testis development,which embodied reduced testis mass and slowed spermatogenesis in transgenic mice. Moreover,transgenic mice showed increase of the percentage of broken sperm. The further study revealed that the reproductive capacity was impaired in GAT1 overexpressing mice. In addition,testosterone level was significantly low in transgenic mice compared with that in wild-type mice. Our findings provided the first evidence that abnormal expression of GAT1 could result in dysgenesis,and indicated that GAT1 might be therapeutically targeted for contraception or dysgenesis treatment. | JiaHuaHU JinFuZHANG YingHuaMA JieJIANG NaYANG XinBoLI ZhiGuangYUCHI JianFEI LiHeGUO | 2004 | Cell Research2004，14，1： | 0 |
| 5 | Transcriptome wide association studies: general framework and methods显示文摘Background:Genome-wide association studies(GWAS)have succeeded in identifying tens of thousands of genetic variants associated with complex human traits during the past decade,however,they are still hampered by limited statistical power and difficulties in biological interpretation.With the recent progress in expression quantitative trait loci(eQTL)studies,transcriptome-wide association studies(TWAS)provide a framework to test for gene-trait associations by integrating information from GWAS and eQTL studies.Results:In this review,we will introduce the general framework of TWAS,the relevant resources,and the computational tools.Extensions of the original TWAS methods will also be discussed.Furthermore,we will briefly introduce methods that are closely related to TWAS,including MR-based methods and colocalization approaches.Connection and difference between these approaches will be discussed.Conclusion:Finally,we will summarize strengths,limitations,and potential directions for TWAS. | Yuhan Xie Nayang Shan Hongyu Zhao Lin Hou | 2021 | Quantitative Biology2021，9，2： | 0 |
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