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| 1 | Molecular Identification and Expression Analysis of Tumor Necrosis Factor Receptor-associated Factor 2 in Grass Carp Ctenopharyngodon idella显示文摘肿瘤坏死因素联系受体的因素(TRAF2 ) 2 是一个关键部件几乎发信号的全部肿瘤坏死因素受体总科小径。在现在的学习, TRAF2 基因从草鲤鱼被克隆 { Ctenopharyngodon idella ) 由反向的抄写聚合酶,链反应和 cDNA 的快速的扩大结束。全身的 cDNA 是 3162 bp ,包括 60 bp 5' untranslated 区域( UTR ), 1611 bp 开的读框架,和 1491 bp 3' UTR.The polyadenylation 信号( AATAAA )和 mRNA 不稳定性主题( ATTTTA , ATTTA )被跟随由一 poly ,在3' UTR.No 信号肽或 transmembrane 区域的(A)尾巴在草鲤鱼 TRAF2 ( gcTRAF2 )的通常认为的氨基酸被发现了。种系发生的树分析清楚地证明 gcTRAF2 几乎金鱼的 TRAF2 基因。有它在从 56% ~ 97%.It 的另外的脊椎动物范围的相当或相同的事物的 gcTRAF2 的身份被一戒指类型签名在 N 终点,在中间的部分的一根锌手指,和由组成的一个保存 TRAF 领域描绘一 C 近似(TRAF-C ) 子域并且一 N 近似(TRAF-N ) 子域。在在脊椎动物的所有 TRAF2 相当或相同的事物之中的 TRAF-C 的身份从 78% ~ 97% 变化,而从 56% ~ 100%.The recombinant gcTRAF2 的 TRAF-N 范围的身份用 pET-32a 表示向量在 Escherichia 关口 i 被表示了。兔子 anti-gcTRAF2 polyclonal 抗体被获得。在不同机关的 gcTRAF2 的表示被即时量的聚合酶链反应和蛋白质印迹分析检验。它广泛地在心,头肾,胸腺,大脑,鳃,肝,怒气,和箱子肾被散布。这是在鱼的一个 TRAF2 相当或相同的事物分子的第一份报告。 | Zaiyan XU Pin NIE Baojian SUN Mingxian CHANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，11： | 6 |
| 2 | Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel(Monopterus albus)显示文摘The Asian swamp eel(Monopterus albus) is one of the most economically important freshwater fish in East Asia,but data on the immune genes of M.albus are scarce compared to other commercially important fish.A better understanding of the eel's immune responses may help in developing strategies for disease management,potentially improving yields and mitigating losses.In mammals,interferon regulatory factors(IRFs) play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRF10 have seldom been studied.In this study,we characterized IRF4 and IRF10 from M.albus(maIRF4 and maIRF10) and found that maIRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid(aa) protein,while maIRF10 consists of 1 744 nucleotides including an open reading frame(ORF) of 1 236 nt encoding 411 aa.The maIRF10 gene was constitutively expressed at high levels in a variety of tissues,while maIRF4 showed a very limited expression pattern.Expression of maIRF4 and maIRF10 in head kidney,and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic: polycytidylic acid(poly I:C),lipopolysaccharide(LPS) and a common pathogenic bacteria Aeromonas hydrophila.These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M.albus. | Qiao-Qing XU Dai-Qin YANG Rui TUO Jing WAN Ming-Xian CHANG Pin NIE | 2014 | Zoological Research2014，35，5： | 3 |
| 3 | Identification of suppressor of cytokine signalling (SOCS) 6, 7, 9 and CISH in rainbow trout Oncorhynchus mykiss and analysis of their expression in relation to other known trout SOCS显示文摘 | Tiehui Wang Qian Gao Pin Nie Christopher J. Secombes | 2010 | Fish and Shellfish Immunology2010，，4： | 1 |
| 4 | DEVELOPMENT OF EUSTRONGYLIDES IGNOTUS (NEMATODA: DIOCTOPHMIDA) IN DOMESTIC DUCKS ANAS PLATYRHYNCHOS DOMESTICA (L.))显示文摘 | Xiong Fan Wang Gui Tang Wu Shan Gong Nie Pin | 2009 | The Journal of Parasitology2009，，： | 1 |
| 5 | Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation显示文摘 | Jingyun Tian Baojian Sun Yanping Luo Yongan Zhang Pin Nie | 2008 | Aquaculture2008，，1： | 1 |
| 6 | Origin and evolution of the RIG-I like RNA helicase gene family 显示文摘 | Zou Jun Chang Ming-xian Nie Pin | 2009 | BMC Evol Biol2009，9，： | 1 |
| 7 | Immunogenic proteins and their vaccine development potential evaluation in outer membrane proteins(OMPs)of Flavobacterium columnare显示文摘Flavobacterium columnare is a Gram-negative bacterium that causes columnaris disease in freshwater fish worldwide.Many studies have focused on the identification of protective antigens to aid in the development of novel vaccines against the disease.In this study,an immunoblotting approach was employed to identify immunogenic outer membrane proteins(OMPs)from F.columnare in two-dimensional electrophoresis(2-DE)map gels using antibacterial sera obtained from grass carp(Ctenopharyngodon idella),and anti-grass carp-recombinant Ig(rIg)monoclonal antibodies.Five unique immunogenic proteins,including the gliding motility lipoprotein GldJ(GldJ),hypothetical protein FCOL_13420(Fco1),lipoprotein(Lip),F0F1 ATP synthase subunit beta(F0f1)and outer membrane efflux protein precursor(Omep),were characterized.Over-expression of these proteins in Escherichia coli DE3,and their immunogenicity and protective efficacy were evaluated in grass carp.The relative percent survival(RPS)of the groups immunized separately with recombinant GldJ,Lip and Omep was 72%,64%and 68%,respectively when compared to control fish.Up-regulation of immuno-related genes and specific antibodies were detected in immunized fish and sera of immunized fish inhibited the growth of F.columnare.The results suggest that GldJ,Lip and Omep are major protective antigens and may be considered as novel candidates in the development of vaccines against columnaris disease in fish. | Zhang Luo Jianping Fu Nan Li Zhixing Liu Ting Qin Xiaolin Zhang Pin Nie | 2016 | Aquaculture and Fisheries2016，1，1： | 1 |
| 8 | Cryptic genetic diversity and host specificity of Bothriocephalus acheilognathi Yamaguti,1934(Eucestoda:Bothriocephalidea)显示文摘In this report, genetic variation and phylogeny of B. acheilognathi were analyzed based on 96 samples collected from 20 fish host species in 29 different localities by using the r DNA internal transcribed spacer(ITS) sequences. The ITS1 and ITS2 sequences of B. acheilognathi varied among 561–639 bp and 559–648 bp, and ten simple sequence repeat loci(microsatellites) were detected in the ITS regions, which contributed to significant sequence length variation. Phylogenetic analyses revealed 4 genetic clades(A, B, C, D) in samples with significantly different fish host spectra and geographic distribution. Clade A possessed a wide host range and geographic distribution, including all the samples of B. acheilognathi determined in previous report. Clades B, C, and D only infected the small cyprinid fishes Opsariichthys bidens and Zacco platypus, and were limited to different locality. Interesting, Clades A and D were detected coexisting in same water body, and even in same fish host O. bidens in Danjiangkou Reservoir. The relatively higher genetic divergence and wide geographic distribution of B. acheilognathi parasitic in O. bidens indicated that O. bidens is likely to be the primary host. Sympatric occurrence of the two genetically distinct clades suggests the possibility of allopatric speciation and second encounter events. | Bingwen Xi Wenxiang Li Guitang Wang Pin Nie Jun Xie | 2016 | Zoological Systematics2016，41，2： | 0 |
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