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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | Plasminogen kringle 5-engineered glioma cells block migration of tumor- associated macrophages and suppress tumor vascularization and progres- sion.显示文摘 | Perri SR Nalbantoglu J Annabi B Koty Z Lejeune L Francois M Di Falco MR Beliveau R Galipeau J | 2005 | 中国神经肿瘤杂志2005，3，3： | 8 |
| 3 | The role of the N-methyl-D-aspartate receptor in the proliferation of adult hippocampal neural stem and precursor cells显示文摘New neurons are continuously generated from resident pools of neural stem and precursor cells(NSPCs)in the adult brain.There are multiple pathways through which adult neurogenesis is regulated,and here we review the role of the N-methyl-D-aspartate receptor(NMDAR)in regulating the proliferation of NSPCs in the adult hippocampus.Hippocampal-dependent learning tasks,enriched environments,running,and activity-dependent synaptic plasticity,all potently up-regulate hippocampal NSPC proliferation.We first consider the requirement of the NMDAR in activity-dependent synaptic plasticity,and the role the induction of synaptic plasticity has in regulating NSPCs and newborn neurons.We address how specific NMDAR agonists and antagonists modulate proliferation,both in vivo and in vitro,and then review the evidence supporting the hypothesis that NMDARs are present on NSPCs.We believe it is important to understand the mechanisms underlying the activation of adult neurogenesis,given the potential that endogenous stem cell populations have for repopulating the hippocampus with functional new neurons.In conditions such as age-related memory decline,neurodegeneration and psychiatric disease,mature neurons are lost or become defective;as such,stimulating adult neurogenesis may provide a therapeutic strategy to overcome these conditions. | TAYLOR Chanel J HE RongQiao BARTLETT Perry F | 2014 | Science China(Life Sciences)2014，57，4： | 6 |
| 4 | 心血管疾病病死率与血清25-羟基维生素D浓度相关性研究显示文摘目的研究心衰死亡、心血管疾病死亡和过早死亡与血清25-羟基维生素D[25(OH)D]浓度的相关性。方法在第3次美国健康和营养调查中,1988~1994年为基线数据,纳入35岁以上参与者13 131名(男性6130名,女性7 001名),随访至2000年12月31日。结果随访8年期间,共有3 266人死亡(24.9%),其中心衰死亡101人,心血管疾病死亡1 451人,早死1 066人。心衰死亡者中有37%血清25(OH)D水平低于20ng/mL,非心衰死亡者为26%(P<0.001)。多元Cox模型显示,血清25(OH)D水平低于20ng/mL的心衰死亡风险是超过30ng/mL的2.06倍(95%CI:1.01~4.25)(P<0.001),过早死亡风险为1.40倍(95%CI:1.17~1.68;P<0.001);血清25(OH)D浓度20~29ng/mL的过早死亡风险是超过30ng/mL的1.11倍(95%CI:0.93~1.33)。结论血清25(OH)D水平不足的成年人死于心血管病、心衰和过早死亡的风险较高。 | 刘隆健 魏敏 陈明 Shelley R Hankins Ana E.Nùez Robert A Watson Perry J Weinstock Craig J.Newschaffer Howard J Eisen 邹强 杨书 陈卫中 | 2013 | 成都医学院学报2013，8，2： | 6 |
| 5 | Aspirin responsive platelet thrombophilia in essential thrombocythemia and polycythemia vera显示文摘Essential thrombocythemia(ET) and polycythemia vera(PV) frequently present with erythromelalgia and acrocyanotic complications, migraine-like microvascular cerebral and ocular transient ischemic attacks(MIAs) and/or acute coronary disease. The spectrum of MIAs in ET range from poorly localized symptoms of transient unsteadiness, dysarthria and scintillating scotoma to focal symptoms of transient monocular blindness, transient mono- or hemiparesis or both. The attacks all have a sudden onset, occur sequentially rather than simultaneously, last for a few seconds to several minutes and are usually associated with a dull, pulsatile or migraine-like headache. Increased hematocrit and blood viscosity in PV patients aggravate the microvascular ischemic syndrome of thrombocythemia to major arterial and venous thrombotic complications. Phlebotomy to correct hematocrit to normal in PV significantly reduces major arterial and venous thrombotic complications, but fails to prevent the platelet-mediated erythromelalgia and MIAs. Complete long-term relief of the erythromelalgic microvascular disturbances, MIAs and major thrombosis in ET and PV patients can be obtained with low dose aspirin and platelet reduction to normal, but not with anticoagulation. Skin punch biopsies from the erythromelalgic area show fibromuscular intimal proliferation of arterioles complicated by occlusive plateletrich thrombi leading to acrocyanotic ischemia. Symptomatic ET patients with erythromelalgic microvascular disturbances have shortened platelet survival, increased platelet activation markers β-thromboglobulin(β-TG), platelet factor 4(PF4) and thrombomoduline(TM), increased urinary thromboxane B2(TXB2) excretion, and no activation of the coagulation markers thrombin fragments F1+2 and fibrin degradation products. Inhibition of platelet cyclooxygenase(COX1) by aspirin is followed by the disappearance and no recurrence of microvascular disturbances, increase in platelet number, correction of the shortened platelet survival times to normal, and reduction of increased plasma levels of β-TG, PF4, TM and urinary TXB2 excretion to normal. These results indicate that platelet-mediated fibromuscular intimal proliferation and platelet-rich thrombi in the peripheral, cerebral and coronary end-arterial microvasculature are responsible for the erythromelalgic ischemic complica-tions, MIAs and splanchnic vein thrombosis. Baseline platelet P-selectin levels and arachidonic acid induced COX1 mediated platelet activation showed a highly significant increase of platelet P-selectin expression(not seen in ADP and collagen stimulated platelets), which was significantly higher in JAK2V617 F mutated compared to JAK2 wild type ET. | Jan Jacques Michiels Fibo WJ Ten Kate Peter J Koudstaal Perry JJ Van Genderen | 2013 | World Journal of Hematology2013，2，2： | 4 |
| 6 | MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development显示文摘轴突开发从细胞内部的供应要求膜增加,它被显示了被 Rab10 积极的 plasmalemmal 先锋泡(PPV ) 调停。然而,位于 PPV 的膜 trafficking 过程下面的分子的机制仍然保持不清楚。这里,我们显示出那 myristoylated 充满丙氨酸的 C-kinase 底层(MARCKS ) 调停膜指向 Rab10 积极的 PPV,和这条规定为轴突开发是批评的。我们发现 Rab10 的锁 GTP 的活跃形式绑在联系膜的 MARCKS,其亲密关系取决于 MARCKS 受动器领域的 phosphorylation 地位。MARCKS 的或基因的 silencing 或它和 Rab10 的相互作用的混乱禁止了外皮的神经原的轴突生长,停靠损害并且有血浆膜的 Rab10 泡的熔化,并且因而引起了对细胞外的轴突生长因素应答的 axonal 受体的膜插入的损失。因此,这研究在调停识别了 MARCKS 的新奇功能在轴突开发期间 PPV 指向的膜。 | Xiao-Hui Xu Cai-Yun Deng Yang Liu Miao He Jian Peng Tong Wang Lei Yuan Zhi-Sheng Zheng Perry J Blackshear Zhen-Ge Luo | 2014 | Cell Research2014，24，5： | 2 |
| 7 | Identification of Gene Expression–Based Prognostic Markers in the Hematopoietic Stem Cells of Patients With Myelodysplastic Syndromes显示文摘 | Andrea Pellagatti Axel Benner Ken I. Mills Mario Cazzola Aristoteles Giagounidis Janet Perry Luca Malcovati Matteo G. Della Porta Martin J?dersten Amit Verma Emma-Jane McDonald Sally Killick Eva Hellstr?m-Lindberg Lars Bullinger James S. Wainscoat Jacquel | 2013 | Journal of Clinical Oncology2013，，28： | 2 |
| 8 | Organic optical limiter with a strong nonlinear absorptive response 显示文摘 | PERRY J W MANSOUR K LEE I-Y S | 1996 | Science1996，273，： | 2 |
| 9 | Perception, attention, and working memory are disproportionately impaired in dementia with Lewy bodies compared to Alzheimer's disease显示文摘 | Perry RJ Erzinclioglu SW | 2001 | J Neurol Neurosurg Psychiatry2001，70，： | 1 |
| 10 | Remote Estimation of Sell Moisture Availability and Fractional Vegetation Cover for Agricultural Fields显示文摘 | Perry E M Schmugge T J | 1990 | Agriculture and Forest Meteorology1990，52，12： | 1 |
| 11 | Prospective study of serum total homocysteine concentration and risk of stroke in middle-aged British men显示文摘 | PERRY I J REFSUM H MORRIS R W | 1995 | Lancet1995，346，8987： | 1 |
| 12 | The economics of metal ion implantation 显示文摘 | James R Treglio Athony J Perry Rober J Stinner | 1994 | Surface and Coatings Technology1994，，65： | 1 |
| 13 | Design of high-efficiency dielectric reflection gratings显示文摘 | Shore B W Perry M D Britten J A | 1997 | J Opt Soc Am A1997，14，： | 1 |
| 14 | The effect of usage and size on tractor depreciation显示文摘 | Perry G M Bayaner A Nixon C J | 1990 | American Journal of Agricultural Economics1990，72，2： | 1 |
| 15 | Illustrations and guidelines for selecting statistical methods for quantifying spatial pattern in ecological data显示文摘 | Perry J N Liebhold A M Rosenberg M S | 2002 | Ecography2002，25，5： | 1 |
| 16 | Biomechanical evaluation ofkyphoplasty with calcium sulfate cement in a cadaveric osteoporot-ic vertebral compression fracture model 显示文摘 | Perry A Mahar A Massie J | 2005 | Spine2005，5，5： | 1 |
| 17 | Surface and Coatings Technology显示文摘 | Perry A J Baouchi A W Petersen J H | 1992 | 54- 55:261 - 2651992，5455，： | 1 |
| 18 | Content Familiarity: Differential Impact of Effective Teaching on Student Achievement Outcomes 显示文摘 | Schonwetter D J Clifton R A Perry R P | 2002 | Research in Higher Education2002，43，6： | 1 |
| 19 | Villoglandular papillary adenocarcinoma of the uterine cervix:a diagnostic challenge显示文摘 | Korach J Machtinger R Perri T | 2009 | Acta Obstetricia et Gynecologica Scandinavica2009，88，3： | 1 |
| 20 | The value of T2 relaxation times to characterize lumbar interverbral disks: preliminary results显示文摘 | Perry J Haughton V Anderson PA | 2006 | Am J Neuroradiol2006，27，4： | 1 |
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