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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | The effect of usage and size on tractor depreciation显示文摘 | Perry G M Bayaner A Nixon C J | 1990 | American Journal of Agricultural Economics1990，72，2： | 1 |
| 3 | Glomerulosclerosis: aparaneoplastic phenomenon? 显示文摘 | LOI S PERRY G J STANDISH H | 2004 | Nephrology (Carlton)2004，9，6： | 1 |
| 4 | Intrinsically superhydrophobic organosilica sol-gel foams显示文摘 | Shirtcliffe N J McHale G Perry C C | 2003 | Langmuir2003，19，14： | 1 |
| 5 | Biological feedbacks to climate change: terrestrial ecosystems as sinks and sources of carbon and nitrogen显示文摘 | Perry D A Borchers J G Turner D P | 1991 | The Northwest Environmental Journal1991，7，： | 1 |
| 6 | Genome-wide identifica- tion of direct target genes implicates estrogen-related receptorct as a de- terminant of breast cancer heterogeneity 显示文摘 | DEBLOIS G HALL J A PERRY M-C | 2009 | Cancer Res2009，69，15： | 1 |
| 7 | Antioxidant Protection and Neurodegenerative Disease: the Role of Amyloid-beta and Tau显示文摘 | Castellani R J Lee HG'Perry G Smith MA | 2006 | Am J Alzheimers Dis Other Demen2006，21，12： | 1 |
| 8 | 2001 Percutaneous injury rates显示文摘 | Perry Parker G Jagger J | 2003 | Advances in Exposure Prevention2003，6，3： | 1 |
| 9 | 2001 Percutaneous injury rates 显示文摘 | Perry J Parker G Jagger J | 2003 | Adv Expo Prey2003，6，3： | 1 |
| 10 | Interleukin-2 activity in patients with extensive small cell lung cancer:a phase Ⅱ trial of cancer and leukemia group B 显示文摘 | Clamon G Herndon J Perry MC | 1993 | J Natl Cancer Inst1993，85，4： | 1 |
| 11 | Parkinsen ' s disease is associated with oxidative damage to cytoplasmic DNA and RNA in substantia nigra neurons 显示文摘 | Zhang J Perry G Smith MA | 1999 | Am J Pathol1999，154，5： | 1 |
| 12 | Oak tree hazard e- valuation显示文摘 | Hickman G W Caprile J Perry E | 1989 | Journal of Arboriculture1989，15，： | 1 |
| 13 | Rapid estimation of canopy nitrogen of cereal crops at paddock scale using a canopy chlorophyll content index显示文摘 | Eileen M Perry Glenn J Fitzgerald James G | 2012 | Field Crops Research2012，134，： | 1 |
| 14 | On Inflation and Inflation Uncertainty in G7 Countries 显示文摘 | Kevin B G Perry M J | 1998 | Journal of International Money and Finance1998，，17： | 1 |
| 15 | Neurotrophin-3 Promotes Proliferation of Olfactory Ensheathing Cells from Human Nose显示文摘 | Bianco J I Perry C Harkin D G | 2004 | Gila2004，45，： | 1 |
| 16 | Intrinsically super-hydrophobic organ-silica solgel foams显示文摘 | Shirtcliffe N J Mc Hale G Newton MI Perry CC | 2003 | Langmuir2003，19，14： | 1 |
| 17 | Entrepreneurial effectuation:A review and suggestions for future research显示文摘 | Perry J T Chandler G N Markova G | 2012 | Entrepreneurship Theory and Practice2012，36，4： | 1 |
| 18 | Oxidative stress in Alzheimer disease: a possibility for prevention显示文摘 | Bonda D J Wang X Perry G | 2010 | Neuropharmacology2010，59，45： | 1 |
| 19 | The evolution of new lipoprotein subunits of the bacterial outer membrane BAM complex显示文摘 | Anwari K Webb CT Poggio S Perry A J Belousoff M Celik N Ramm G Lovering A Sockett RE Smit J | | 0，，05： | 1 |
| 20 | Characterization of a manganese-containing catalase from the obligate thermophile Thermo- leophilum album显示文摘 | ALLGOOD G S PERRY J J | 1986 | Journal of bacteriology1986，168，： | 1 |
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