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| 1 | 变性梯度凝胶电泳(DGGE)在微生物生态学中的应用显示文摘由于从环境样品中分离和培养细菌的困难 ,分子生物学方法已发展用来描述和鉴定微生物群落。近年来基于 DNA方法的群落分析得到了迅速的发展 ,如 PCR扩增技术 ,克隆文库法 ,荧光原位杂交法 ,限制性酶切片段长度多态性法 ,变性和温度梯度凝胶电泳法。DGGE已广泛用于分析自然环境中细菌、蓝细菌 ,古菌、微微型真核生物、真核生物和病毒群落的生物多样性。这一技术能够提供群落中优势种类信息和同时分析多个样品。具有可重复和容易操作等特点 ,适合于调查种群的时空变化 ,并且可通过对切下的带进行序列分析或与特异性探针杂交分析鉴定群落成员。DGGE分析微生物群落的一般步骤如下 :一是核酸的提取 ,二是 1 6Sr RNA,1 8S r RNA或功能基因如可容性甲烷加单氧酶羟化酶基因 ( mmo X)和氨加单氧酶 α-亚单位基因 ( amo A)片段的扩增 ,三是通过DGGE分析 PCR产物。DGGE使用具有化学变性剂梯度的聚丙烯酰胺凝胶 ,该凝胶能够有区别的解链PCR扩增产物。由 PCR产生的不同的 DNA片段长度相同但核苷酸序列不同。因此不同的双链 DNA片段由于沿着化学梯度的不同解链行为将在凝胶的不同位置上停止迁移 [5] 。 DNA解链行为的不同导致一个凝胶带图案 ,该图案是微生物群落中主要种类的一个轮廓。 DGGE使用所有生物中保守的基因? | 马悦欣 Carola Holmstrm Jeremy Webb Staffan Kjelleberg | 2003 | 生态学报2003，23，8： | 100 |
| 2 | Non-invasive diagnosis of liver fibrosis and cirrhosis显示文摘The evaluation and follow up of liver fibrosis and cirrhosis have been traditionally performed by liver biopsy. However, during the last 20 years, it has become evident that this 'gold-standard' is imperfect; even according to its proponents, it is only 'the best' among available methods. Attempts at uncovering non-invasive diagnostic tools have yielded multiple scores, formulae, and imaging modalities. All are better tolerated, safer, more acceptable to the patient, and can be repeated essentially as often as required. Most are much less expensive than liver biopsy. Consequently, their use is growing, and in some countries the number of biopsies performed, at least for routine evaluation of hepatitis B and C, has declined sharply. However, the accuracy and diagnostic value of most, if not all, of these methods remains controversial. In this review for the practicing physician, we analyze established and novel biomarkers and physical techniques. We may be witnessing in recent years the beginning of the end of the first phase for the development of non-invasive markers. Early evidence suggests that they might be at least as good as liver biopsy. Novel experimental markers and imaging techniques could produce a dramatic change in diagnosis in the near future. | Yoav Lurie Muriel Webb Ruth Cytter-Kuint Shimon Shteingart Gerardo Z Lederkremer | 2015 | World Journal of Gastroenterology2015，21，41： | 36 |
| 3 | 南非国家高中数学课程与评价标准评介显示文摘2012年1月,南非颁布新的高中数学课程标准,较之与以往的南非课程标准相比,新的高中课程标准最为突出的变化体现在对学生评价的重视.在该课程标准中,除对课程颁布的背景、课程目标、课时分配、课程内容有详细介绍之外,还重点强调了对学生学习成就评价的标准.介绍南非高中数学课程标准及其特点,以期为中国高中数学课程改革提供借鉴. | 李娜 曹一鸣 Lyn Webb | 2013 | 数学教育学报2013，22，4： | 25 |
| 4 | 黄连素片对钙离子通道的拮抗作用显示文摘本研究以药理学的方法检测了黄连素片对Ca2+通道的拮抗作用。结果表明黄连素片的水溶液能明显抑制Ca2+的内流.拮抗L型钙通道促进剂Bayk8644对钙通道的激活,从而降低SD大鼠胸主动脉的收缩性,40μg/ml浓度的黄连素片对Ca2+通道的抑制作用与10-8mol/L浓度的尼群地平相似。同时用Bayk8644使SD大鼠胸主动脉收缩后,黄连素片能以剂量依赖方式引发收缩动脉的自发性舒张。80mg/L浓度的黄连素片能使10-7mol/LBayk8644所致的收缩血管基本达到100%的舒张,其作用与10-7mol/L的尼群地平相似。说明黄连素片具有抑制电压控制的钙通道的作用,这可能是它具有心血管药理作用的基础。 | 司晓晨 Webb,RC | 1996 | 南京中医药大学学报1996，12，6： | 24 |
| 5 | 牛腔前卵泡在体外无血清培养中发育为有腔卵泡显示文摘用无血清培养系统研究了牛腔前卵泡的体外培养。直径为 10 0~ 2 0 0μm的牛腔前卵泡在添加 L -谷氨酰胺、BSA、睾酮、转铁蛋白和硒的 Mc Coy′s5 a培养液中培养 ,卵泡保持正常的形态结构并持续生长 ,培养 10 d左右形成卵泡腔 ,成腔率约 5 0 %。培养液中添加胰岛素对卵泡直径的增长和卵泡腔的形成有明显的促进作用 ,但添加 FSH对腔前卵泡的生长未表现促进作用。 | 周虚 Robert Webb | 2002 | 中国兽医学报2002，22，5： | 18 |
| 6 | Effect of resistance training on non-alcoholic fatty-liver disease a randomized-clinical trial显示文摘AIM:To evaluate the effect of resistance training(RT)on non alcoholic liver disease(NAFLD)patients.METHODS:A randomized clinical trial enrolling NAFLD patients without secondary liver disease(e.g.,without hepatitis B virus,hepatitis C virus or excessive alcohol consumption).Patients were randomly allocated either to RT,three times weekly,for 3 mo or a control arm consisting of home stretching.The RT included leg press,chest press,seated rowing,latissimus pull down etc.with 8-12 repetitions,3 sets for each exercise,for a total duration of 40 min.Hepatic ultrasound,fasting blood tests,anthropometrics and body composition by dual energy X-ray absorptiometry were assessed.At baseline and follow-up,patients filled out a detailed semi-quantitative food frequency questionnaire reporting their habitual nutritional intake.Steatosis was quantified by the hepatorenal-ultrasound index(HRI)representing the ratio between the brightness level of the liver and the right kidney.The HRI has been previously demonstrated to be highly reproducible and was validated against liver biopsy and proton magnetic resonance spectroscopy.RESULTS:Eighty two patients with primary NAFLD were randomized to receive 3 mo of either RT or stretching.After dropout or exclusion from analysis because of protocol violation(weight change>3 kg),thirty three patients in the RT arm and 31 in the stretching arm completed the study per protocol.All baseline characteristics were similar for the two treatment groups with respect to demographics,anthropometrics and body composition,blood tests and liver steatosis on imaging.HRI score was reduced significantly in the RT arm as compared to the stretching arm(-0.25±0.37 vs-0.05±0.28,P=0.017).The RT arm had a significantly higher reduction in total,trunk and android fat with increase in lean body mass.There was no correlation between the reduction in HRI in the RT arm and weight change during the study,but it was positively correlated with the change in trunk fat(r=0.37,P=0.048).The RT arm had a significant reduction in serum ferritin and total cholesterol.There was no significant difference between arms in dietary changes and these did not correlate with HRI change.CONCLUSION:Three months RT improves hepatic fat content accompanied by favorable changes in body composition and ferritin.RT may serve as a complement to treatment of NAFLD. | Shira Zelber-Sagi Assaf Buch Hanny Yeshua Nahum Vaisman Muriel Webb Gil Harari Ofer Kis Naomi Fliss-Isakov Elena Izkhakov Zamir Halpern Erwin Santo Ran Oren Oren Shibolet | 2014 | World Journal of Gastroenterology2014，20，15： | 17 |
| 7 | 施用不同土壤改良剂对砖红壤酸度的影响显示文摘对砖红壤施用不同的改良剂,经一年的大田试验,测定土壤的活性酸与交换酸研究不同土壤改良剂对酸性土壤酸度的影响。试验结果表明:滤泥单施与石灰单施处理能有效降低土壤的活性酸以及交换性酸的含量,有较好的降低土壤酸度的效果,而施用绿肥、甘蔗渣、羊粪以及羊粪与膨润土混施均不能有效降低土壤的酸度。 | 郇恒福 刘国道 Suzane Berthelsane Michae Webb 李运祥 | 2009 | 热带作物学报2009，30，8： | 16 |
| 8 | 施用不同土壤改良剂对砖红壤交换性能影响的初步研究显示文摘对砖红壤施用不同的改良剂,进行1a的大田试验后,测定土壤的阳离子交换量、有效阳离子交换量、盐基离子总量以及盐基饱和度来研究不同土壤改良剂对酸性土壤交换性能的影响。结果表明:施用滤泥(40t/hm2)、石灰(2.5t/hm2)以及在施用一定量的羊粪(20t/hm2)基础上施用中量(40t/hm2)或者高量(60t/hm2)膨润土的复合改良剂是有效提升酸性土壤交换性能、增加土壤盐基总量与盐基饱和度的优良措施。 | 郇恒福 刘国道 Michael Webb Suzane Berthelsane 李运祥 | 2009 | 热带作物学报2009，30，11： | 12 |
| 9 | RhoA/Rho激酶在大鼠阴茎勃起机制中的调节作用显示文摘 一般认为一氧化氮(nitricoxide,NO)释放增加促进小动脉和海绵体平滑肌舒张是导致男性阴茎勃起的主要机制.研究发现内源性血管收缩因子对维持阴茎萎软状态有重要作用.应用大鼠模型活体检测使用Y 27632拮抗Rho 激酶活性后对阴茎勃起生理机制的影响;应用免疫转印技术检测阴茎海绵体内RhoA和Rho激酶蛋白的表达.结果显示在大鼠海绵体组织内有内源性RhoA和Rho激酶蛋白的表达和存在;Y 27632海绵体内注射阻滞RhoA/Rho激酶活性使ICP和CCP/MAP比值明显升高;局部小剂量应用Y 27632对MAP没有明显影响;Y 27632可增强系列电刺激引起的由NO介导的CCP/MAP比值的增加;NO合成酶和鸟苷酸环化酶抑制剂的作用不能阻滞RhoA/Rho激酶抑制剂对大鼠阴茎海绵体平滑肌的松弛作用和CCP/MAP的增加.说明RhoA/Rho激酶信号系统发挥了维持海绵体萎软状态重要作用,这是与NO介导途径不同的阴茎勃起生理调节机制.RhoA/Rho激酶抑制剂可能是ED治疗的新领域新方法. | 戴玉田 孙则禹 Thomas M.Mills Kanchan Chitaley Webb R.Clinton Ronald W.Lewis | 2002 | 南京大学学报(自然科学版)2002，38，5： | 11 |
| 10 | 强化安保体系建设 确保人员财产安全--乍得项目成功撤离经验分析显示文摘面对乍得共和国长期存在的战乱风险,中油国际(乍得)有限责任公司牢固树立'以人为本'的理念,高度重视安保体系建设。项目公司成立独立安保部,确保安保工作常态化,构建多渠道信息源,建立风险评估体系,配备四级通讯设备和必要的应急物资,加强沟通和交流等,从及时掌握最新的安全动态,加强预警,确保物资与资金投入,争取当地员工和政府的理解和支持等几方面确保安保体系的建设,成功地确保2008.2.1事件应急有序,紧急安全撤离。 | 窦立荣 齐金郦 刘烈强 李西权 韩启凤 王博 russ john webb | 2009 | 中国安全生产科学技术2009，5，S1： | 10 |
| 11 | Relationship between vascular endothelium and periodontal disease in atherosclerotic lesions: Review article显示文摘Inflammation and endothelial dysfunction are linked to the pathogenesis of atherosclerotic disease. Recent studies suggest that periodontal infection and the ensuing increase in the levels of inflammatory markers may be associated with myocardial infarction, peripheral vascular disease and cerebrovascular disease. The present article aimed at reviewing contemporary data on the pathophysiology of vascular endothelium and its association with periodontitis in the scenario of cardiovascular disease. | Marco Aurélio Lumertz Saffi Mariana Vargas Furtado Carisi Anne Polanczyk Márlon Munhoz Montenegro Ingrid Webb Josephson Ribeiro Cassio Kampits Alex Nogueira Haas Cassiano Kuchenbecker Rosing Eneida Rejane Rabelo-Silva | 2015 | World Journal of Cardiology2015，7，1： | 9 |
| 12 | 回热器对制冷系统性能的影响显示文摘通过对制冷系统中回热器、蒸发器和压缩机工作过程的理论分析以及对蒸发器的热设计,定量地讨论了回热器出入口条件的改变对蒸发器尺寸、压缩机功率以及制冷系统成绩系数等的影响. 降低制冷工质在回热器出口处的过热度,可以减小蒸发器的尺寸,但由此带来的不利因素,是需要增加制冷工质的质量流量以及提高压缩机的压缩功率. | 吴晓敏 R L Webb 王维城 | 2001 | 上海理工大学学报2001，23，3： | 8 |
| 13 | Comparison of fatty liver index with noninvasive methods for steatosis detection and quantification显示文摘AIM:To compare noninvasive methods presently used for steatosis detection and quantification in nonalcoholic fatty liver disease(NAFLD).METHODS:Cross-sectional study of subjects from the general population,a subgroup from the First Israeli National Health Survey,without excessive alcohol consumption or viral hepatitis.All subjects underwent anthropometric measurements and fasting blood tests.Evaluation of liver fat was performed using four noninvasive methods:the SteatoTest;the fatty liver index(FLI);regular abdominal ultrasound(AUS);and the hepatorenal ultrasound index(HRI).Two of the noninvasive methods have been validated vs liver biopsy and were considered as the reference methods:the HRI,the ratio between the median brightness level of the liver and right kidney cortex;and the SteatoTest,a biochemical surrogate marker of liver steatosis.The FLI is calculated by an algorithm based on triglycerides,body mass index,γ-glutamyl-transpeptidase and waist circumference,that has been validated only vs AUS.FLI < 30 rules out and FLI ≥ 60 rules in fatty liver.RESULTS:Three hundred and thirty-eight volunteers met the inclusion and exclusion criteria and had valid tests.The prevalence rate of NAFLD was 31.1% according to AUS.The FLI was very strongly correlated with SteatoTest(r = 0.91,P < 0.001) and to a lesser but significant degree with HRI(r = 0.55,P < 0.001).HRI and SteatoTest were significantly correlated(r = 0.52,P < 0.001).The κ between diagnosis of fatty liver by SteatoTest(≥ S2) and by FLI(≥ 60) was 0.74,which represented good agreement.The sensitivity of FLI vs SteatoTest was 85.5%,specificity 92.6%,positive predictive value(PPV) 74.7%,and negative predictive value(NPV) 96.1%.Most subjects(84.2%) with FLI < 60 had S0 and none had S3-S4.The κ between diagnosis of fatty liver by HRI(≥ 1.5) and by FLI(≥ 60) was 0.43,which represented only moderate agreement.The sensitivity of FLI vs HRI was 56.3%,specificity 86.5%,PPV 57.0%,and NPV 86.1%.The diagnostic accuracy of FLI for steatosis > 5%,as predicted by SteatoTest,yielded an area under the receiver operating characteristic curve(AUROC) of 0.97(95% CI:0.95-0.98).The diagnostic accuracy of FLI for steatosis> 5%,as predicted by HRI,yielded an AUROC of 0.82(95% CI:0.77-0.87).The κ between diagnosis of fatty liver by AUS and by FLI(≥ 60) was 0.48 for the entire sample.However,after exclusion of all subjects with an intermediate FLI score of 30-60,the κ between diagnosis of fatty liver by AUS and by FLI either ≥ 60 or < 30 was 0.65,representing good agreement.Excluding all the subjects with an intermediate FLI score,the sensitivity of FLI was 80.3% and the specificity 87.3%.Only 8.5% of those with FLI < 30 had fatty liver on AUS,but 27.8% of those with FLI ≥ 60 had normal liver on AUS.CONCLUSION:FLI has striking agreement with SteatoTest and moderate agreements with AUS or HRI.However,if intermediate values are excluded FLI has high diagnostic value vs AUS. | Shira Zelber-Sagi Muriel Webb Nimer Assy Laurie Blendis Hanny Yeshua Moshe Leshno Vlad Ratziu Zamir Halpern Ran Oren Erwin Santo | 2013 | World Journal of Gastroenterology2013，19，1： | 8 |
| 14 | 抑制平滑肌的张力收缩:一种治疗勃起功能障碍的新途径显示文摘阴茎海绵体血管的收缩及张力的维持是由钙敏感的RhoA/Rho激酶信号传导途径介导的。以Y 2 76 32抑制海绵体平滑肌细胞的Rho激酶信号系统可促进阴茎勃起 ,表现为海绵体内压 (ICP)明显升高 ,而平均动脉压 (MAP)变化不显著。为什么在血管收缩信号很强的情况下性刺激仍能引发阴茎勃起反应 ?我们设想体内一氧化氮 (NO)能直接抑制RhoA/Rho激酶途径的活性 ,减少血管张力 ,促使血管舒张 ,勃起发生 ,使用Y 2 76 32可以恢复性腺功能低下和高血压病动物ED模型的阴茎勃起功能 ,这表明抑制Rho激酶活性药物具有临床应用的前景。此外 ,我们的结果显示阴茎表面使用Rho激酶抑制剂可能为ED治疗的有效方法之一。 | 戴玉田 Thomas M MILLS Ronald W LEWIS RClinton WEBB | 2002 | 中华男科学杂志2002，8，6： | 8 |
| 15 | Prevalence of vitreous floaters in a community sample of smartphone users显示文摘AIM: To estimate the prevalence and risk factors for vitreous floaters in the general population. ·METHODS: An electronic survey was administered through a smartphone app asking various demographic and health questions, including whether users experience floaters in their field of vision. Multivariate logistic regression analysis was used to determine risk factors. ·RESULTS: A total of 603 individuals completed the survey, with 76% reporting that they see floaters, and 33% reporting that floaters caused noticeable impairment in vision. Myopes were 3.5 times more likely (P =0.0004), and hyperopes 4.4 times more likely (P =0.0069) to report moderate to severe floaters compared to those with normal vision. Floater prevalence was not significantly affected by respondent age, race, gender, and eye color. ·CONCLUSION: Vitreous floaters were found to be a very common phenomenon in this non -clinical general population sample, and more likely to be impairing in myopes and hyperopes. | Blake F. Webb Jadon R. Webb Mary C. Schroeder Carol S. North | 2013 | International Journal of Ophthalmology(English edition)2013，6，3： | 7 |
| 16 | Liver transplantation for alcoholic liver disease显示文摘Alcoholic liver disease(ALD) is the second commonest indication for liver transplantation after viral hepatitis in the United States and Europe.Controversies surround the indications and allocation of scarce and expensive resource for this so called self inflicted disease.Controversies stem from the apprehension that alcoholic recipients are likely to relapse and cause damage to the graft.There is a need to select those candidates with lower risk for relapse with the available predictive factors and scores.Substance abuse specialist and psychiatrists are mandatory in the pre-transplant evaluation and in the post-transplant follow-up.There is conflicting evidence to support a fixed period of pretransplant abstinence,although most units do follow this.Alcoholic hepatitis(AH) continues to be a contraindication for transplantation,however there is a need for further research in this f ield as a subset of patients with AH who do not respond to medical treatment,have high early mortality and could benefit from transplantation.One year,3-year,and 5-year survival post-transplant is similar for both ALD and non-ALD recipients.The incidence of post-transplant rejection and retransplantation is also similar to other recipients.ALD with viral hepatitis especially hepatitis C virus leads to a more aggressive liver disease with early presentation for transplantation.ALD patients are more prone to develop de-novo malignancy;this is attributed to the long term effect of alcohol,tobacco combined with immunosuppression.Post-transplant surveillance is important to detect early relapse to alcoholism,presence of de-novo malignancy and treat the same adequately. | Vibha Varma Kerry Webb Darius F Mirza | 2010 | World Journal of Gastroenterology2010，16，35： | 6 |
| 17 | Role of illness perception and self-efficacy in lifestyle modification among non-alcoholic fatty liver disease patients显示文摘AIM To describe the relationships between non-alcoholic fatty-liver disease(NAFLD) patient's disease consequences and treatment perceptions, self-efficacy, and healthy lifestyle maintenance. METHODS A cross-sectional study among 146 ultrasound diagnosed NAFLD patients who visited the fatty liver clinic at the TelAviv Medical Center. Eighty-seven of these individuals, participated in a clinical trial of physical activity and underwent fasting blood tests, analyzed at the same lab. Exclusion criteria included positivity for serum HBsA g or anti-HCV antibodies; fatty liver suspected to be secondary to hepatotoxic drugs; excessive alcohol consumption(≥ 30 g/d in men or ≥ 20 g/d in women) and positive markers of genetic or immune-mediated liver diseases. Patients were asked to complete a selfreport structured questionnaire, assembled by the Israeli Center for Disease Control. Nutrition habits were measured using six yes/no questions(0 = no, 1 = yes) adopted from the national survey questionnaire. Participants in the clinical trial completed a detailed semi-quantitative food frequency questionnaire(FFQ) reporting their habitual nutritional intake during the past year. Self-efficacy was assessed by the Self-Efficacy Scale questionnaire, emotional representation, degree of illness understanding, timeline perception, treatment perception and symptoms were measured by the Brief Illness Perception questionnaire. Illness consequences were measured by the Personal Models of Diabetes Interview questionnaire. A path analysis was performed to describe the interrelationships between the patients' illness perceptions, and assess the extent to which the data fit a prediction of nutritional habits.RESULTS The study sample included 54.1% men, with a mean age of 47.76 ± 11.68 years(range: 20-60) and mean body mass index of 31.56 ± 4.6. The average perceived nutrition habits score was 4.73 ± 1.45 on a scale between 0-6, where 6 represents the healthiest eating habits. Most of the study participants(57.2%) did not feel they fully understood what NAFLD is. Better nutritional habits were positively predicted by the degree of illness understanding(β = 0.26; P = 0.002) and selfefficacy(β = 0.25; P = 0.003). Perceptions of more severe illness consequences were related with higher emotional representation(β = 0.55; P < 0.001), which was related with lower self-efficacy(β =-0.17; P = 0.034). The perception of treatment effectiveness was positively related with self-efficacy(β = 0.32; P < 0.001). In accordance with the correlation between self-efficacy and the perceived nutrition habits score, self-efficacy was also correlated with nutrient intake evaluated by the FFQ; negatively with saturated fat(percent of saturated fat calories from total calories)(r =-0.28, P = 0.010) and positively with fiber(r = 0.22, P = 0.047) and vitamin C intake(r = 0.34, P = 0.002). In a sub analysis of the clinical trial participants, objectively measured compliance to physical activity regimen was positively correlated with the self-efficacy level(r = 0.34, P = 0.046). CONCLUSION Self-efficacy and illness understanding are major determinants of lifestyle-modification among NAFLD patients. This information can assist clinicians in improving compliance with lifestyle changes among these patients. | Shira Zelber-Sagi Shiran Bord Gali Dror-Lavi Matthew Lee Smith Samuel D Towne Jr Assaf Buch Muriel Webb Hanny Yeshua Assy Nimer Oren Shibolet | 2017 | World Journal of Gastroenterology2017，23，10： | 6 |
| 18 | On the radiogenic heat production of igneous rocks显示文摘Radiogenic heat production is a physical parameter crucial to properly estimating lithospheric temperatures and properly understanding processes related to the thermal evolution of the Earth. Yet heat production is, in general, poorly constrained by direct observation because the key radiogenic elements exist in trace amounts making them difficulty image geophysically. In this study, we advance our knowledge of heat production throughout the lithosphere by analyzing chemical analyses of 108,103 igneous rocks provided by a number of geochemical databases. We produce global estimates of the average and natural range for igneous rocks using common chemical classification systems. Heat production increases as a function of increasing felsic and alkali content with similar values for analogous plutonic and volcanic rocks. The logarithm of median heat production is negatively correlated(r^2=0.98)to compositionally-based estimates of seismic velocities between 6.0 and 7.4 km s^(-1), consistent with the vast majority of igneous rock compositions. Compositional variations for continent-wide models are also well-described by a log-linear correlation between heat production and seismic velocity. However, there are differences between the log-linear models for North America and Australia, that are consistent with interpretations from previous studies that suggest above average heat production across much of Australia. Similar log-linear models also perform well within individual geological provinces with^1000 samples. This correlation raises the prospect that this empirical method can be used to estimate average heat production and natural variance both laterally and vertically throughout the lithosphere. This correlative relationship occurs despite a direct causal relationship between these two parameters but probably arises from the process of differentiation through melting and crystallization. | D. Hasterok J. Webb | 2017 | Geoscience Frontiers2017，8，5： | 5 |
| 19 | 应用Rho激酶抑制剂对大鼠阴茎勃起的作用显示文摘目的 :探讨将RhoA/Rho激酶抑制剂Y 2 76 32涂抹于阴茎白膜表面以及阴茎龟头皮肤表面有无促进阴茎勃起的作用及其对体循环的影响。 方法 :2 0只成年雄性SD大鼠 ,体重为 2 5 0~ 30 0g ,随机分为实验组和对照组。全身麻醉后颈动脉和海绵体插管连续监测平均动脉压 (MAP)和阴茎海绵体内压 (CCP)的变化。寻找盆腔星状神经节(MPG)并以系列电刺激诱发勃起。向海绵体白膜表面及阴茎龟头皮肤表面涂抹Y 2 76 32 ,观察用药前后阴茎勃起的改变及MAP的改变。 结果 :将Rho激酶抑制剂Y 2 76 32涂抹于大鼠阴茎白膜表面和阴茎龟头皮肤表面均可促进在无阴茎支配神经电刺激下发生勃起 (ICP/MAP明显升高 ) ,在系列电刺激MPG时用药后发生的勃起反应较用药前亦明显增强。表面用药后还发现大鼠体循环血压有不同程度的降低。 结论 :阴茎皮肤表面应用Rho激酶抑制剂是一种安全有效的ED治疗方法 。 | 戴玉田 孙则禹 Chitaley K Webb RC Mills TM Lewis RW | 2004 | 中华男科学杂志2004，10，8： | 5 |
| 20 | 牛腔前卵泡的体外培养显示文摘用无血清培养系统研究了牛腔前卵泡的体外培养。直径为 1 0 0~ 2 0 0 μm的牛腔前卵泡在添加L -谷氨酸、BSA、睾酮、转铁蛋白和硒的McCoy’s 5a培养液中培养 ,卵泡保持正常的形态结构并持续生长。培养的第 1 0d左右形成卵泡腔 ,成腔率约50 %。培养液中添加胰岛素 ,对卵泡直径的增长和卵泡腔的形成有明显的促进作用 。 | 周虚 Robert Webb | 2001 | 草食家畜2001，，S2： | 5 |
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