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| 1 | Recent advances on electrochemical methods in fabricating two-dimensional organic-ligand-containing frameworks显示文摘Organic-ligand-containing frameworks have drawn considerable attention due to their multifunctional properties as well as tunable structures for broad applications.Among numerous synthesis methods developed in the past two decades,electrochemical processing has been demonstrated as one of the most efficient,safe,and facile ways to realize the large-scale and highly controllable production of organic-ligand-containing frameworks.In this review,the progress of electrochemically induced crystallization and thin film fabrication of organic-ligand-containing frameworks is summarized in a well-rounded way.Besides,the mechanism and processing parameters are also discussed.Moreover,the main challenges are also expounded for providing some guidance on the future development of organic-ligand-containing frameworks,especially for covalent-organic frameworks and hydrogen-bonded organic frameworks. | Xiang Wang Pengfei She Qichun Zhang | 2021 | SmartMat2021，2，3： | 2 |
| 2 | Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides显示文摘Anemarrhena asphodeloides is an immensely popular medicinal herb in China,which contains an abundant of mangiferin.As an important bioactive xanthone C-glycoside,mangiferin possesses a variety of pharmacological activities and is derived from the cyclization reaction of a benzophenone C-glycoside(maclurin).Biosyntheti-cally,C-glycosyltransferases are critical for the formation of benzophenone C-glycosides.However,the benzo-phenone C-glycosyltransferases from Anemarrhena asphodeloides have not been discovered.Herein,a promiscuous C-glycosyltransferase(AaCGT)was identified from Anemarrhena asphodeloides.It was able to catalyze efficiently mono-C-glycosylation of benzophenone,together with di-C-glycosylation of dihydrochalcone.It also exhibited the weak O-glycosylation or potent S-glycosylation capacities toward 12 other types of flavonoid scaffolds and a simple aromatic compound with–SH group.Homology modeling and mutagenesis experiments revealed that the glycosylation reaction of AaCGT was initiated by the conserved residue H23 as the catalytic base.Three critical residues H356,W359 and D380 were involved in the recognition of sugar donor through hydrogen-bonding interactions.In particular,the double mutant of F94W/L378M led to an unexpected enzy-matic conversion of mono-C-to di-C-glycosylation.This study highlights the important value of AaCGT as a potential biocatalyst for efficiently synthesizing high-value C-glycosides. | Jia Huang Yaru She Jingyang Yue Yidu Chen Yu Li Jing Li Yonger Hu Deying Yang Jiabo Chen Lu Yang Zhongqiu Liu Ruibo Wu Pengfei Jin Lixin Duan | 2022 | Synthetic and Systems Biotechnology2022，7，1： | 1 |
| 3 | In Situ Control of Multicolor Luminescence over the Entire Visible Spectral Region in a Single Chromophore by Sol–Gel Transformation and Dynamic Metal–Ligand Coordination显示文摘Controlling multicolor luminescence in a single chromophore is of fundamental significance but remains a great challenge.In this study,a carbazole group was incorporated into terpyridine through acylamide to produce a novel chromophore(N-{4-[(2,2′:6′,2″-terpyridine)-4′-yl]benzyl}-9-hexyl-9H-carbazole-3-carboxamide,TBCC)for achieving programmable luminescence switches.Significantly different emission states,such as nonemissive,blue,green,yellow,red,and white,were achieved in situ by controlling sol–gel transformation with sonication/heat and the reversible metal–ligand coordination between TBCC and various metal salts. | Yun Ma Fan Yu Shujun Zhang Pengfei She Shujuan Liu Wei Huang Qiang Zhao | 2021 | CCS Chemistry2021，3，9： | 0 |
| 4 | PA1426 regulates Pseudomonas aeruginosa quorum sensing and virulence:an in vitro study显示文摘Objective:Pseudomonas aeruginosa(P.aeruginosa)contains a hierarchy of quorum sensing(QS)network,consisting of the las,rhl and pqs systems,which play a key role in coordinating the expression of virulence factors.PA2146 was found to be associated with P.aeruginosa pathogenicity in macrophage and host Immune response.The aim of this study was to investigate the effects of PA2146 on the virulence of P.aeruginosa and explore its mechanism.Methods:PA2146 gene knockout strain and complement strain of P.aeruginosa PAO1 were constructed.The biomass of biofilm was detected by crystal violet staining;the virulence factors were measured,including pyocyanin,rhamnolipid,LasA elastase,LasB elastase and hemolytic activity;RNA-seq and label-free relative quantitative proteomics analyses were carried out to test the influence of PA2146 on transcriptomics and proteomics.This study was approved by the Institutional Review Board of the Third Xiangya Hospital,Central South University,China(approval No.2019-S021).Results:PA2146-deficient strains showed reduced biofilm formation and increased pyocyanin,rhamnolipid,LasA elastase,LasB elastase,and hemolytic activity,as well as increased motility,compared with the wild-type strain.RNA-seq and label-free relative quantitative proteomics analyses revealed that PA2146 repressed the transcription of several genes that are integral to the pqs system and to pyocyanin biosynthesis,and increased the expression of MexEF-OprN efflux pump components at the gene and protein level.Conclusion:PA2146 gene was found to inhibit the pqs system.PA2146 may affect quorum sensing by directly inhibiting the pqs system or by enhancing the expression of MexEF-OprN efflux pump components,thereby promoting efflux of 2-heptyl-4(1H)-quinolone,a Pseudomonas quinolone signal precursor,and thus affecting P.aeruginosa virulence. | Fang Tan Pengfei She Linying Zhou Shijia Li Xianghai Zeng Lanlan Xu Yaqian Liu Zubair Hussain Yong Wu | 2021 | Journal of Bio-X Research2021，4，1： | 0 |
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