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| 1 | Detection of serum TNF-α,IFN-γ,IL-6 and IL-8 in patients with hepatitis B显示文摘NTRODUCTIONSinceMuto[1]reportedthatTNFαandIL1wererelatedtofulminanthepatitis,thestudiesontherelationshipbetweencytokinesa... | WANG Jing Yan, WANG Xue Lian and LIU Pei | 1999 | World Journal of Gastroenterology1999，5，1： | 54 |
| 2 | EXPERIMENTAL STUDY OF FLOWFIELD STRUCTURE AROUND AN OGIVE-CYLINDER显示文摘The flowfield structure and their aerodynamic characteristics over an ogive cylinder were studied by means of flow visualization and surface pressure measurement in a water tunnel and a wind tunnel. The existence of multi asymmetric vortices over long slender bodies was experimentally confirmed at large angles of attack and in the subcritical Reynolds number range. The spatial 3 D characteristics of the multi vortices system were analyzed and a physical model was developed. The topological structure of different patterns in cross flow plane was studied and the mechanism governing the formation of asymmetric vortices and multi vortices was discussed from the viewpoint of stability of the topological structure. It was concluded that the maximum in the sectional side force distribution curve are not caused by the shedding of higher position vortex, but by the cross over to the symmetric plane of the lower position vortex. | Ph.D.Candidate:Wang Gang Fluid Mechanics Institute, Beijing University of Aeronautics and Astronautics, Beijing 100083, China,Supervisor: Deng Xue ying (Beijing University of Aeronautics and Astronautics) Members of Dissertation Defense Committee: Tong Bing gang (Graduate School of the Chinese Academy of Sciences),Chairman Gu Zhi fu (Peking University) Yang Qi de (China Aerodynamics Research and Development Center) Lv Zhi yong (Beijing University of Aeronautics and Astronautics) Liu Pei qing (Beijing University of Aeronautics and Astronautics) | 2003 | Journal of Hydrodynamics2003，15，5： | 18 |
| 3 | Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system显示文摘 | Pei Zhao Zhe Zhang Hongmei Ke Yiren Yue Ding Xue | 2014 | Cell Research2014，24，2： | 17 |
| 4 | Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells显示文摘To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesen-chymal stem cells (BMSCs) were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissue- engineered skin. The results showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle shape to round or oval; tonofibrils, melano-somes and keratohyaline granules were observed under a transmission electronic microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% ± 4.2%) and epidermal cells differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06 pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material short-ened skin wound repair significantly. In one word, autogenic BMSCs have the potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering. | HE LiJuan, NAN Xue, WANG YunFang, GUAN LiDong, BAI CiXian, SHI ShuangShuang, YUAN HongFeng, CHEN Lin, LIU DaQing & PEI XueTao Laboratory of Stem Cells and Regenerative Medicine, Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China | 2007 | Science China(Life Sciences)2007，50，4： | 16 |
| 5 | Craniocerebral injury promotes the repair of peripheral nerve injury显示文摘The increase in neurotrophic factors after craniocerebral injury has been shown to promote fracture healing. Moreover, neurotrophic factors play a key role in the regeneration and repair of peripheral nerve. However, whether craniocerebral injury alters the repair of peripheral nerve injuries remains poorly understood. Rat injury models were established by transecting the left sciatic nerve and using a free-fall device to induce craniocerebral injury. Compared with sciatic nerve injury alone after 6–12 weeks, rats with combined sciatic and craniocerebral injuries showed decreased sciatic functional index, increased recovery of gastrocnemius muscle wet weight, recovery of sciatic nerve ganglia and corresponding spinal cord segment neuron morphologies, and increased numbers of horseradish peroxidase-labeled cells. These results indicate that craniocerebral injury promotes the repair of peripheral nerve injury. | Wei Wang Jun Gao Lei Na Hongtao Jiang Jingfeng Xue Zhenjun Yang Pei Wang | 2014 | Neural Regeneration Research2014，9，18： | 15 |
| 6 | Characteristics of the lunar samples returned by the Chang'E-5 mission显示文摘Forty-five years after the Apollo and Luna missions returned lunar samples,China's Chang’E-5(CE-5)mission collected new samples from the mid-latitude region in the northeastern Oceanus Procellarum of the Moon.Our study shows that 95%of CE-5 lunar soil sizes are found to be within the range of 1.40-9.35μm,while 95%of the soils by mass are within the size range of 4.84-432.27μm.The bulk density,true density and specific surface area of CE-5 soils are 1.2387 g/cm^(3),3.1952 g/cm^(3) and 0.56 m^(2)/g,respectively.Fragments from the CE-5 regolith are classified into igneous clasts(mostly basalt),agglutinate and glass.A few breccias were also found.The minerals and compositions of CE-5 soils are consistent with mare basalts and can be classified as low-Ti/low-Al/low-K type with lower rare-earth-element contents than materials rich in potassium,rare earth element and phosphorus.CE-5 soils have high FeO and low Mg index,which could represent a new class of basalt. | Chunlai Li Hao Hu Meng-Fei Yang Zhao-Yu Pei Qin Zhou Xin Ren Bin Liu Dawei Liu Xingguo Zeng Guangliang Zhang Hongbo Zhang Jianjun Liu Qiong Wang Xiangjin Deng Caijin Xiao Yonggang Yao Dingshuai Xue Wei Zuo Yan Su Weibin Wen Ziyuan Ouyang | 2022 | National Science Review2022，9，2： | 12 |
| 7 | Passive-source ocean bottom seismograph(OBS) array experiment in South China Sea and data quality analyses显示文摘Long-term passive source ocean bottom seismograph(OBS) observatory is challenging due to various technical difficulties. In order to gain experience in this field, and to reveal the lithospheric structure beneath the extinct ridge in the central South China Sea(SCS), we carried out a passive source OBS array experiment, which includes 18 OBSs, in the deep portion of SCS. Here we present the instrumentation, the OBS deployment and recovery of this experiment, and more importantly, the data quality evaluated by a number of approaches. Through processing and inspecting waveforms from global, regional and local earthquakes, we find that most of recovered OBSs have good data quality with discernible main phases. The ambient noise analyses of OBS recordings show that their noise is higher than the global average, and the horizontal component is noisier than the vertical, indicating current impacts on horizontal components are more severe. In the period range of 5–10 s, there is a noise notch for the SCS OBSs, and noise levels of horizontal components are comparable to the vertical. This feature, which is not seen at OBS stations in open ocean, suggests the distant sources for double frequency microseism in this marginal sea are not significant. In addition, we successfully determined the orientations for 7 OBSs by investigating their Rayleigh wave polarizations; and we demonstrated the dispersion feature of Rayleigh waves through the frequency-time analysis. Finally, we summarized lessons learned from this experiment regarding the passive source OBS investigations in SCS. | Chenguang Liu Qingfeng Hua Yanliang Pei Ting Yang Shaohong Xia Mei Xue Ba Manh Le Da Huo Fang Liu Haibo Huang | 2014 | Chinese Science Bulletin2014，59，33： | 11 |
| 8 | Modifi cation of wheat bran insoluble and soluble dietary fibers with snail enzyme显示文摘Insoluble dietary fiber(IDF)and soluble dietary fiber(SDF)extracted from wheat bran were modifi ed by snail enzyme and their physicochemical properties(water retention capacity and oil retention capacity),functional properties(cholesterol adsorption capacity,glucose adsorption capacity and antioxidant activity)and structural characterizations were evaluated.The results showed that snail enzyme modification led to the significant increase in oil retention capacity of IDF,glucose adsorption capacity and cholesterol adsorption capacity of IDF and SDF.Enzymatic modification also markedly improved the DPPH radical scavenging capacity and reducing power of IDF and SDF.Meanwhile,scanning electron microscopy(SEM)analysis indicated the microstructures of IDF and SDF powders were signifi cantly changed.Fourier transfer-infrared spectrometry(FT-IR)showed that snail enzyme modifi cation could degrade the part of cellulose and hemicellulose of IDF and SDF.All these improved physicochemical and functional properties of IDF and SDF might depend on their structural changes.It suggested that snail enzyme modifi cation could effectively improve physicochemical and functional properties of IDF and SDF from wheat bran. | Xin Liu Keke Suo Pei Wang Xue Li Limin Hao Jiaqing Zhu Juanjuan Yi Qiaozhen Kang Jinyong Huang Jike Lu | 2021 | Food Science and Human Wellness2021，10，3： | 10 |
| 9 | Variable Selection for Semiparametric Varying-Coefficient Partially Linear Models with Missing Response at Random显示文摘在这份报纸,我们在场由把基础功能近似与为 semiparametric 变化系数估计方程惩罚相结合的一个可变选择过程有失踪的反应在的部分线性的模型随机。建议过程同时在参量的部件和 nonparametric 部件选择重要变量。与调节参数的适当选择,我们建立可变选择过程的一致性和调整评估者的集中率。模拟研究被承担估计建议可变选择过程的有限样品表演。 | Pei Xin ZHAO Liu Gen XUE | 2011 | Acta Mathematica Sinica,English Series2011，27，11： | 9 |
| 10 | Machine learning assisted design of γ′-strengthened Co-base superalloys with multi-performance optimization显示文摘Designing a material with multiple desired properties is a great challenge,especially in a complex material system.Here,we propose a material design strategy to simultaneously optimize multiple targeted properties of multi-component Co-base superalloys via machine learning.The microstructural stability,γ′solvus temperature,γ′volume fraction,density,processing window,freezing range,and oxidation resistance were simultaneously optimized. | Pei Liu Haiyou Huang Stoichko Antonov Cheng Wen Dezhen Xue Houwen Chen Longfei Li Qiang Feng Toshihiro Omori Yanjing Su | 2020 | npj Computational Materials2020，，1： | 8 |
| 11 | Productive HBV infection of well-differentiated, hNTCP-expressing human hepatoma-derived(Huh7) cells显示文摘Feasible and effective cell models for hepatitis B virus(HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol(DMSO/PEG), h NTCP expression, and a differentiated state are the limiting factors for successful HBV infection models. In the present study, we used a hepatoma cell line(Hu7^(hDNTCPh)) to overcome these limiting factors so that it exhibits excellent susceptibility to HBV infection. To achieve this goal, different hepatoma cell lines were tested with 2.5% DMSO/4%PEG8000, and one resistant cell line(Huh7 D) was used to construct a stable h NTCP-expressing cell line(Hu7^(hDNTCPh)) using a recombinant lentivirus system. Then, the morphological characteristics and differentiation molecular markers of Hu7^(hDNTCPh) cells with or without DMSO treatment were characterized. Finally, the susceptibility of Hu7^(hDNTCPh) cells to HBV infection was assessed. Our results showed that Huh7 D cells were resistant to 2.5% DMSO/4% PEG8000, whereas the others were not. Hu7^(hDNTCPh) cells were established to express a high level of h NTCP compared to liver extracts, and Hu7^(hDNTCPh) cells rapidly transformed into a non-dividing, well-differentiated polarized phenotype under DMSO treatment. Hu7^(hDNTCPh) cells fully supported the entire lifecycle of HBV infection. This cell culture system will be useful for the analysis of host-virus interactions, which should facilitate the discovery of antiviral drugs and vaccines. | Ming Zhou Kaitao Zhao Yongxuan Yao Yifei Yuan Rongjuan Pei Yun Wang Jizheng Chen Xue Hu Yuan Zhou Xinwen Chen Chunchen Wu | 2017 | Virologica Sinica2017，32，6： | 7 |
| 12 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 13 | Serum paraoxonase 1 heteroplasmon, a fucosylated, and sialylated glycoprotein in distinguishing early hepatocellular carcinoma from liver cirrhosis patients显示文摘浆液 glycoproteins 的异常 glycan 结构在 hepatocellular 癌(HCC ) 的不同阶段创造唯一的模式,它为 HCC 的早诊断提供潜在的 glycan biomarkers。在这研究,双人脚踏车 lectin 亲密关系层析使用 aleuria aurantia lectin ( AAL )和小麦细菌凝集素( WGA )被处理从 27 肝肝硬化( LC )和 27 个早 HCC 病人净化 fucosylated 和 sialylated 浆液 glycoproteins ,在哪个 122 glycoproteins 被液体层析双人脚踏车团 spectrometry ( LC-MSMS )最后外面屏蔽。在 LC-MSMS 识别的 122 蛋白质之中,他们中的 8 个仅仅在 HCC 浆液被识别, 6 仅仅在 LC 浆液存在。浆液 paraoxonase (PON1 ) 1 是从 47 个单个病人的 immunoprecipitated 并且在 LC 比那些在 HCC 浆液由 lectins,出现提高的 fucosylation 和 sialylation 弄污浆液。在为 AAL 反应的 PON1 的 ROC 曲线(AUROC ) 下面的区域在把早 HCC 区分开来与 LC 与 71.4% 的敏感和 94.7% 的特性是 0.892。同样, WGA 反应的 PON1 与 95.2% 的敏感和 78.9% 的特性有 0.902 的 AUROC。数据显示浆液 PON1 的 glycan 差别可能为把早 HCC 与 LC 病人区分开来提供同样潜在的 glycan biomarkers。 | Chun Sun Pei Chen Qiaopei Chen Lu Sun Xiaonan Kang Xue Qin Yinkun Liu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，9： | 6 |
| 14 | A new single-cell protein from Clostridium autoethanogenum as a functional protein for largemouth bass(Micropterus salmoides)显示文摘Clostridium autoethanogenum protein(CAP)is a new single-cell protein source originating from inactivated bacteria.An in vitro digestion experiment and an 8-wk growth experiment were conducted to evaluate the molecular weight distribution of the CAP hydrolysate,and the effects of dietary CAP levels on the growth performance,plasma parameters,hepatic and intestinal health,and the diversity of gutadherent microbiota of largemouth bass(Micropterus salmoides).The fish(initial body weight of 47.99±0.01 g)were fed diets where CAP gradually replaced 0%(CAP0),12.5%(CAP12.5),25%(CAP25),37.5%(CAP37.5)and 50%(CAP50)of low-temperature steam dried anchovy fish meal(LTFM)in the diet.Results showed that the content of peptides below 1,000 Da in the CAP hydrolysate(0.56 mg/mL)was higher than that of the LTFM hydrolysate(0.48 mg/mL).Dietary CAP inclusion had no negative effect on growth performance,while whole-body lipid content significantly reduced in the CAP25 and CAP50 groups(P<0.05).The plasma alanine aminotransferase activities and triglyceride concentrations in the CAP inclusion groups were significantly lower than those in the CAP0 group(P<0.05).The plasma aspartate aminotransferase activity was significantly reduced in the CAP37.5 group(P<0.05).The richness and diversity of the gut-adhesive microbiota and the proportion of Clostridium sensu stricto 12 in the CAP50 group were significantly higher than those in the CAP0 group(P<0.05).Dietary CAP inclusion inhibited inflammatory responses by down-regulating the mRNA levels of interleukin 1b(IL1b),IL10 and transforming growth factor b1(P<0.05)in the liver.The mRNA levels of acetyl-CoA carboxylase 1 were significantly down-regulated in the CAP12.5,CAP25 and CAP37.5 groups(P<0.05),while that of fatty acid synthase was significantly down-regulated in the CAP50 group(P<0.05).These results demonstrate that dietary CAP inclusion could improve the hepatic and intestinal health of largemouth bass,and can be helpful to further develop CAP as a functional feed ingredient. | Shifeng Ma Xiaofang Liang Pei Chen Jie Wang Xu Gu Yuchang Qin Christophe Blecker Min Xue | 2022 | Animal Nutrition2022，，3： | 6 |
| 15 | In vitro differentiation of human adipose-derived mesenchymal stem cells into endothelial-like cells显示文摘The neovascularization of ischemic tis- sue is a crucial initial step for the functional rehabili- tation and wound healing. However, the short of seed cell candidate for the foundation of vascular network is still a big issue. Human adipose tissue derived mesenchymal stem cells (hADSCs), which possess multilineage potential, are capable of adipogenic, osteogenic, and chondrogenic differentiation. We examined whether this kind of stem cells could dif- ferentiate into endothelial-like cells and participate in blood vessel formation, and whether they could be used as an ideal cell source for therapeutic angio- genesis in ischemic diseases or vascularization of tissue constructs. The results showed that hADSCs, grown under appropriately induced conditions, dis- played characteristics similar to those of vessel en- dothelium. The differentiated cells expressed endo- thelial cell markers CD34 and vWF, and had high metabolism of acetylated low-density lipoprotein and prostacyclin. In addition, the induced cells were able to form tube-like structures when cultured on matrigel. Our data indicated that induced hADSCs could ex- hibit characteristics of endothelial cells. Therefore, these cells, as a source of human endothelial cells, may find many applications in such realms as engi- neering blood vessels, endothelial cell transplantation for myocardial regeneration, and induction of angio- genesis for treatment of regional ischemia. | GUAN Lidong LI Shaoqing WANG Yunfang YUE Huimin LIU Daqing HE Lijuan BAI Cixian YAN Fang NAN Xue SHI Shuangshuang PEI Xuetao | 2006 | Chinese Science Bulletin2006，51，15： | 6 |
| 16 | Au core-PtAu alloy shell nanowires for formic acid electrolysis显示文摘Inefficient electrocatalysts and high-power consumption are two thorny problems for electrochemical hydrogen(H2)production from acidic water electrolysis.Herein we report the one-pot precise synthesis of ultrafine Au core-Pt Au alloy shell nanowires(Au@PtxAu UFNWs).Among them,Au@Pt_(0.077) Au UFNWs exhibit the best performance for formic acid oxidation reaction(FAOR)and hydrogen evolution reaction(HER),which only require applied potentials of 0.29 V and-22.6 m V to achieve a current density of 10 m A cm^(-2),respectively.The corresponding formic acid electrolyzer realizes the electrochemical H2 production at a voltage of only 0.51 V with 10 m A cm^(-2) current density.Density functional theory(DFT)calculations reveal that the Au-riched Pt Au alloy structure can facilitates the direct oxidation pathway of FAOR and consequently elevates the FAOR activity of Au@Pt_(0.077) Au UFNWs.This work provides meaningful insights into the electrochemical H_(2) production from both the construction of advanced bifunctional electrocatalysts and the replacement of OER. | Qi Xue Xin-Yu Bai Yue Zhao Ya-Nan Li Tian-Jiao Wang Hui-Ying Sun Fu-Min Li Pei Chen Pujun Jin Shi-Bin Yin Yu Chen | 2022 | Journal of Energy Chemistry2022，31，2： | 5 |
| 17 | Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato显示文摘Streptomyces rubrogriseus HDZ-9-47,isolated from eggs of Meloidogyne spp.,was evaluated as a potential biocontrol agent of Meloidogyne incognita under in vitro and protective field.Microscopic observations showed that HDZ-9-47 parasitized eggs of M.incognita within 7 days.In vitro,the culture filtrate of HDZ-9-47 caused 97.0%mortality of second-stage juveniles(J2s)of M.incognita and inhibited more than 50%egg hatching.In the field,compared with the control,the rootknot index and J2s density in the treatment of drench the broth contained 1012 HDZ-9-47 spores were respectively reduced by 51.1 and 80.7%at 90 days post transplantation,which were better than that in other application doses and methods.In addition,reduction rates of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofumigation was 87.1 and 91.0%,respectively,better than either of HDZ-9-47 or biofumigation used alone or fosthiazate treatment.And tomato yield also increased by 16.1%.Together,our results suggest that HDZ-9-47 could be an effective biocontrol agent of M.incognita,and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for M.incognita control. | JIN Na XUE Hui LI Wen-jing WANG Xue-yan LIU Qian LIU Shu-sen LIU Pei ZHAO Jian-lon JIAN Heng | 2017 | Journal of Integrative Agriculture2017，16，6： | 5 |
| 18 | Crowd queuing simulation with an improved emotional contagion model显示文摘In crowd queuing events, the emotions of individuals are mutually infective, easily confusing the quelling order and possibly leading to crowd events. However, the impact of emotion and personality is not considered in current models of crowd behavior. Other studies focus on social interactions such as emotional transmission [1]. Thus, there is a large difference between the simulation results and the real-life behavior of crowds. To improve the realism of crowd simulation results, we use the knowledge learned from Bosse5S study [2], and propose an emotional contagion method for crowd simulation. | Junxiao XUE Hui YIN Pei LV Mingliang XU Yafei LI | 2019 | Science China(Information Sciences)2019，62，4： | 4 |
| 19 | Phosphatidylserine-Specific Phospholipase A1 is the Critical Bridge for Hepatitis C Virus Assembly显示文摘The phosphatidylserine-specific phospholipase A1(PLA1A)is an essential host factor in hepatitis C virus(HCV)assembly.In this study,we mapped the E2,NS2 and NS5A involved in PLA1A interaction to their lumenal domains and membranous parts,through which they form oligomeric protein complexes to participate in HCV assembly.Multiple regions of PLA1A were involved in their interaction and complex formation.Furthermore,the results represented structures with PLA1A and E2 in closer proximity than NS2 and NS5A,and strongly suggest PLA1 A-E2,s physical interaction in cells.Meanwhile,we mapped the NS5A sequence which participated in PLA1A interaction with the C-terminus of domain 1.Interestingly,these amino acids in the sequence are also essential for viral RNA replication.Further experiments revealed that these four proteins interact with each other.Moreover,PLA1A expression levels were elevated in livers from HCV-infected patients.In conclusion,we exposed the structural determinants of PLA1A,E2,NS2 and NS5A proteins which were important for HCV assembly and provided a detailed characterization of PLA1A in HCV assembly. | Qi Yang Min Guo Yuan Zhou Xue Hu Yun Wang Chunchen Wu Min Yang Rongjuan Pei Xinwen Chen Jizheng Chen | 2019 | Virologica Sinica2019，34，5： | 4 |
| 20 | Evaluation of Thermal Hazard of Composite Modified Double-base Propellant by Microcalorimetery Method显示文摘<正>The thermal decomposition behavior of composite modified double-base(CMDB) propellant containing cyclotrimethylene trinitramine(RDX) was studied via a Calvet microcalorimeter at five different heating rates.The activation energy(E) and the pre-exponential factor(A) of two obvious exothermic processes were obtained by Kissinger's method and Ozawa's method.The entropy of activation(△A~≠),the enthalpy of activation(△H~≠),and the free energy of activation(△G~≠) of the first stage were calculated.To evaluate the thermal hazard of the RDX-CMDB propellant, the critical temperature of thermal explosion(T_b),the self acceleration decomposition temperature(T_(SADT)),the adiabatic decomposition temperature increment(△T_(ad)) and the time-to-explosion of adiabatic system(t) were presented as 145.3℃,138.15℃,1634 K and 583.71 s(n=0) and 586.28 s(n=1),respectively. | XUE Liang ZHAO Feng-qi XING Xiao-ling XU Si-yu PEI Qing GAO Yin HU Rong-zu | 2010 | Chemical Research in Chinese Universities2010，26，4： | 4 |
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