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| 1 | Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification显示文摘AIM:To study whether selected bacterial 16S ribosomal RNA(rRNA)gene phylotypes are capable of disting- uishing irritable bowel syndrome(IBS). METHODS:The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant (IBS-D),eight constipation-predominant(IBS-C)and four mixed symptom-subtype(IBS-M)IBS patients,and fifteen control subjects,were analysed at three time-points with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis(PCA)with linear mixed-effects models applied to the principal component scores. RESULTS:Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component(PC1),explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups(IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05).Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy- lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects(-4.08±0.90 vs -3.33±1.16,P=0.04)and IBS-D and IBS-M subjects (-4.08±0.90 vs-3.08±1.38,P=0.05).Furthermore,a phylotype with 94%similarity to R.torques was more prevalent in IBS-D patients'intestinal micro- biota than in that of control subjects(-2.43±1.49 vs -4.02±1.63,P=0.01).A phylotype with 93%simi- larity to R.torques was associated with control sam- ples when compared with IBS-M(-2.41±0.53 vs -2.92±0.56,P=0.00).Additionally,a R.bromii-like phylotype was associated with IBS-C patients in com- parison to control subjects(-1.61±1.83 vs-3.69± 2.42,P=0.01).All of the above mentioned phylotype specific alterations were independent of the effect of time. CONCLUSION:Significant phylotype level alterationsin the intestinal microbiotas of IBS patients were observed,further emphasizing the possible contribution of the gastrointestinal microbiota in IBS. | Anna Lyra Teemu Rinttil Janne Nikkil Lotta Krogius-Kurikka Kajsa Kajander Erja Malinen Jaana Mtt Laura Mkel Airi Palva | 2009 | World Journal of Gastroenterology2009，15，47： | 26 |
| 2 | Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome显示文摘AIM:To investigate the correlations between selfreported symptoms of irritable bowel syndrome(IBS) and the gastrointestinal(GI) microbiota composition.METHODS:Fecal samples were collected from a total of 44 subjects diagnosed with IBS.Their symptoms were monitored with a validated inflammatory bowel disease questionnaire adjusted for IBS patients.Thirteen quantitative real-time polymerase chain reaction assays were applied to evaluate the GI microbiota composition.Eubacteria and GI bacterial genera(Bifidobacterium,Lactobacillus and Veillonella),groups(Clostridium coccoides/Eubacterium rectale,Desulfovibrio desulfuricans) and distinct bacterial phylotypes [closest 16S rDNA sequence resemblance to species Bifidobacterium catenulatum,Clostridium cocleatum,Collinsella aerofaciens(C.aerofaciens),Coprococcus eutactus(C.eutactus),Ruminococcus torques and Streptococcus bovis ] with a suspected association with IBS were quantified.Correlations between quantities or presence/absence data of selected bacterial groups or phylotypes and various IBSrelated symptoms were investigated.RESULTS:Associations were observed between subjects' self-reported symptoms and the presence or quantities of certain GI bacteria.A Ruminococcus torques(R.torques)-like(94% similarity in 16S rRNA gene sequence) phylotype was associated with severity of bowel symptoms.Furthermore,among IBS subjects with R.torques 94% detected,the amounts of C.cocleatum 88%,C.aerofaciens-like and C.eutactus 97% phylotypes were significantly reduced.Interesting observations were also made concerning the effect of a subject's weight on GI microbiota with regard to C.aerofaciens like phylotype,Bifidobacterium spp.and Lactobacillus spp.CONCLUSION:Bacteria seemingly affecting the symptom scores are unlikely to be the underlying cause or cure of IBS,but they may serve as biomarkers of the condition. | Erja Malinen Lotta Krogius-Kurikka Janne Nikkil Airi Palva Anna Lyra Anne Jskelinen Teemu Rinttil Terttu Vilpponen-Salmela Atte Johannes von Wright | 2010 | World Journal of Gastroenterology2010，16，36： | 13 |
| 3 | F-box protein AFB4 plays a crucial role in plant growth, development and innate immunity显示文摘 | Zhubing Hu Mehmet Ali Kegeli Maria Piisila Jing Li Mantas Survila Pekka Heino Gtinter Brade E Tapio Palva | 2012 | Cell Research2012，22，4： | 7 |
| 4 | Kunitz Trypsin Inhibitor: An Antagonist of Cell Death Triggered by Phytopathogens and Fumonisin B1 in Arabidopsis显示文摘规划房间死亡(PCD ) 是在两植物开发并且在对病原体的植物回答的中央规章的进程。PCD 要求象朊酶并且 suppressors 禁止和 modulating 那样的 pro-apoptotic 因素的并列激活这些过程。在植物,各种各样的象 caspase 一样半胱氨酸朊酶以及丝氨酸朊酶在 PCD 被含有。这里,我们显示出那当在细菌并且在 planta 生产了时, Arabidopsis 的禁止者(KTI1 ) 作为功能的 KTI 扮演的丝氨酸朊酶(Kunitz 胰岛素) 。AtKTI1 的表示响应细菌、真菌的 elicitors 并且到水杨酸酸晚被导致。AtKTI1 基因的 RNAi silencing 与得到 PCD 真菌的毒素 fumonisin B1 (FB1 ) 或带 avrB (Pst avrB ) 的无毒害的细菌的病原体 Pseudomonas syringae pv 西红柿 DC3000 在叶织物的渗入以后导致提高的损害开发。AtKTI1 的 Overexpression 在 Pst avrB 和 FB1 渗入以后导致减少的损害开发。有趣地, AtKTI1 的 RNAi silencing 导致提高的抵抗到剧毒的病原体 Erwinia carotovora subsp。carotovora SCC1,当 AtKTI1 的 overexpression 向这病原体导致更高的危险性时。一起,这些数据显示 AtKTI1 在 plantpathogen 相互作用涉及 modulating PCD。 | Jing Li Günter Brader E. Tapio Palva | 2008 | Molecular Plant2008，1，3： | 3 |
| 5 | Requirement of a Homolog of Glucosidase Ⅱ β-Subunit for EFR-Mediated Defense Signaling in Arabidopsis thaliana显示文摘 | Nina von Numers Mantas Survila Markku Aalto Martine Batoux Pekka Heino E. Tapio Palva Jing Li | 2010 | Molecular Plant2010，3，4： | 2 |
| 6 | Protein Secretion in Bacillus Species显示文摘 | Simonen M Palva I | 1993 | Microbiological Reviews1993，57，： | 1 |
| 7 | The expression signals of the Lactobacillus brevis slpA gene direct eficient heterologous protein production in lactic acid bacteria显示文摘 | Kahala M Palva A | 1999 | Appl Mierobiol Biotechnol1999，51，： | 1 |
| 8 | Salcylic acid induced re- sistance to Erwinia carotovora subsp, carotovora in tobacco显示文摘 | Palva T K Hurtig M Saindrenan P | 1994 | The American Phytopathological Society1994，7，3： | 1 |
| 9 | Isolation and characterization of Laetococcus laetis subsp Laetis promoters显示文摘 | Koivula T Sibakov M Palva I | | 0，，： | 1 |
| 10 | Biological mechanisms of low temperature stress response: Cold acclimation and development of freezing tolerance in plants显示文摘 | Palva E T Htiharju S T Tamminen I Puhakainen T Laitinen R Svensson J | 2002 | JIRCAS Working Report2002，1，23： | 1 |
| 11 | Receptor binding domain of Escherichia coli F18 fimbrial adhesion FedF can be both eficiently secreted and surface displayed in a functional form in Laetococcus lactis显示文摘 | Lindhohn A Smeds A Palva A | 2004 | Appl Environ Microbiol2004，70，： | 1 |
| 12 | Interacting signal path- ways control defense gene expression in Arabidopsis in response to cell wall - degrading enzymes from Erwinia carotovora 显示文摘 | Norman -Setterblad C Vidal S Palva E T | 2000 | Mol Plant - Microbe Interact2000，13，： | 1 |
| 13 | Eleven years prospective follow-up of the tension-free vaginal tape procedure for treatment of stress urinary incontinence显示文摘 | Nilsson CG Palva K Rezapour M | 2008 | Int Urogynecol J Pelvic Floor Dysfunct2008，19，8： | 1 |
| 14 | Molecular control of cold acclimation in trees显示文摘 | WELLING A PALVA E T | 2006 | Physiologia Plantarum2006，127，2： | 1 |
| 15 | MRI-BasedD20/H20-Contrast Method to Study Water Flow andDistribution in Heterogeneous Systems: Demonstration inWood Xylem显示文摘 | Ilvonen K Palva L Peramaki M | 2001 | Journal of Magnetic Resonance2001，149，1： | 1 |
| 16 | Impact of low salt concentra- tion, salt quality on natural large-scale sauerkraut fermen- tation 显示文摘 | Viander B Maki M Palva A | 2003 | Food microbiol2003，20，： | 1 |
| 17 | The expression signals of the Lactobacillus brevis slpA gene direct efficient heterologous protein production in lactic acid bacteria显示文摘 | KAHALA M PALVA A | | 0，，01： | 1 |
| 18 | Molecular control of cold acclimation in trees显示文摘 | WELLING A PALVA E T | 2006 | Physiologia Plantarum2006，127，： | 1 |
| 19 | The WRKY70 transcription factor:a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense显示文摘 | Li J Brader C Palva E T | 2004 | The Plant Cell2004，16，2： | 1 |
| 20 | Long-range temporal correlations and scaling behavior in human brain oscillations显示文摘 | LINKENKAER-HANSENK NIKOULINE V V PALVA J M | 2001 | The Journal of Neuroscience2001，21，4： | 1 |
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