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| 1 | Myofibroblastic cell activation and neovascularization predict native liver survival and development of esophageal varices in biliary atresia显示文摘AIM:To study the relation between collagen 1,α-smooth muscle actin(α-SMA)and CD34 expression and the most essential portoenterostomy(PE)outcomes.METHODS:Liver specimens were obtained at PE from33 biliary atresia(BA)patients for immunohistochemical analysis of collagen 1,α-SMA and CD34.Liver biopsies from 35 organ donors were used as controls.Expression patterns were related to clinical data including age at PE,serum total and conjugated bilirubin concentration at the time of PE and during follow-up,incidence of esophageal varices in follow-up upper gastrointestinal endoscopies,and native liver survival as well as to detailed histopathological findings.RESULTS:Collagen 1(16.4%vs 4.5%,P<0.0001),α-SMA(17.9%vs 4.6%,P<0.0001)and CD34(4.9%vs 3.8%,P=0.017)were markedly overexpressed in BA patients compared with controls.Patients who underwent liver transplantation by age of two years had significantly higher expression of collagen 1(18.6%vs 13.7%,P=0.024),α-SMA(20.4%vs 15.4%,P=0.009)and CD34(5.9%vs 4.0%,P=0.029)at PE compared with native liver survivors.CD34-positive microvessels were identified in the centrizonal region close to central vein in every BA patient.In majority of BA cases(56%)neovascularization was frequent as CD34-positive microvessels were observed in over half of the hepatic lobules.In controls,the CD34-positive microvessels were rare as they were completely absent in 40%and were found in less than 5%of the hepatic lobules in the rest.The difference between BA patients and controls was significant(P<0.0001).Patients who developed esophageal varices by two years had significantly higher expression of CD34 at PE compared with patients without varices(5.6%vs 4.0%,P=0.019).Expression ofα-SMA(r=0.758,P<0.0001)and collagen 1(r=0.474,P=0.016),and the amount of CD34-positive microvessels(r=0.356,P=0.047)were related to patient age at PE.CONCLUSION:Hepatic myofibroblastic cell activation,fibrogenesis and neovascularization are enhanced in BA,progress with increasing PE age and relate to native liver survival and development of esophageal varices. | Janne S Suominen Hanna Lampela Pivi Heikkil Jouko Lohi Hannu Jalanko Mikko P Pakarinen | 2014 | World Journal of Gastroenterology2014，20，12： | 6 |
| 2 | Matrix metalloproteinases in the restorative proctocolectomy pouch of pediatric ulcerative colitis显示文摘AIM:To investigate matrix metalloproteinases(MMPs) and their tissue inhibitors(TIMPs) in pouch mucosa of pediatric onset ulcerative colitis(UC).METHODS:In this cross-sectional study,28 patients with pediatric onset UC underwent ileal pouch biopsy 13 years(median) after proctocolectomy.Expression of MMPs-3,-7,-8,-9,-12 and-26 and TIMPs-1,-2 and-3 in samples was examined using immunohistochemichal methods,and another biopsy was used to evaluate the grade of histological inflammation.Two investigators independently graded the immunohistochemical specimens in a semiquantitative fashion,using a scale marking staining intensity as follows:0 = less than 20 positive cells;1 = 20-50 positive cells;2 = 50-200 positive cells;3 = over 20 positive cells.Fecal calprotectin and blood inflammatory markers [serum C-reactive protein(CRP) and erythrocyte sedimentation rate] were determined during a follow-up visit to examine correlations between these markers and the expression of MMPs and TIMPs.RESULTS:Of the 28 patients with pediatric onset UC,nine had not experienced pouchitis,whereas thirteen reported a single episode,and six had recurrent pouchitis(≥ 4 episodes).At the time of the study,six patients required metronidazole.In all of the others,the most recent episode of pouchitis had occurred over one month earlier,and none were on antibiotics.Only four samples depicted no sign of inflammation,and these were all from patients who had not had pouchitis.Two samples were too small to determine the grade of inflammation,but both had suffered pouchitis,the other recurrent.No sample depicted signs of colonic metaplasia.Most pouch samples showed expression of epithelial(e) and stromal(s) MMP-3(e,n = 22;s,n = 20),MMP-7(e,n = 28;s,n = 27),MMP-12(e,n = 20;s,n =24),TIMP-2(e,n = 23;s,n = 23) and MMP-3(e,n = 23;s,n = 28) but MMP-8(e,n = 0;s,n = 1),MMP-9(e,n = 0;s,n = 9) and MMP-26(e,n = 0;s,n = 3) and TIMP-1(n = 0,both) were lacking.In samples with low grade of inflammatory activity,the epithelial MMP-3 and MMP-7 expression was increased(r =-0.614 and r =-0.472,respectively,P < 0.05 in both).MMPs and TIMPs did not correlate with the markers of inflammation,fecal calprotectin,erythrocyte sedimentation rate,or CRP,with the exception of patients with low fecal calprotectin(< 100 μg/g) in whom a higher expression of epithelial MMP-7 was found no differences in MMPor TIMP-profiles were seen in patients with a history of pouchitis compared to ones with no such episodes.Anastomosis with either straight ileoanal anastomosis or ileoanal anastomosis with J-pouch did depict differences in MMP-or TIMP-expression.CONCLUSION:The expression of MMPs pediatric UC pouch in the long-term shares characteristics with inflammatory bowel disease,but inflammation cannot be classified as a reactivation of the disease. | Laura Mkitalo Maija Piekkala Merja Ashorn Mikko Pakarinen Antti Koivusalo Riitta Karikoski Johanna Natunen Ulpu Saarialho-Kere Risto Rintala Kaija-Leena Kolho | 2012 | World Journal of Gastroenterology2012，18，30： | 2 |
| 3 | Posteriormyotomy/myectomy for persistent stooling problemsin Hirschsprung’s disease显示文摘 | Wildhaber B Pakarinen M Rintala R | 2004 | J Pediatr Surg2004，39，6： | 1 |
| 4 | The effect of organic loading rate and retention time on hydrogen production from a methanogenic CSTR显示文摘 | O pakarinen P kaparaju | 2011 | Bioresour Technol2011，102，19： | 1 |
| 5 | Treatment of pathologicalhumerus shaft fractures with intramedullary nails with or withoutcement fixation 显示文摘 | Laitinen M Nieminen J Pakarinen TK | 2011 | Arch Orthop Trauma Surg2011，131，4： | 1 |
| 6 | The effect of localintrauterine levonorgestrel administration on endometrial thicknessand uterine blood circulation 显示文摘 | Pakarinen P Luukkainen T Laine H | 1995 | Hum Reprod1995，10，9： | 1 |
| 7 | Biliary atresia 显示文摘 | Lampela H Pakarinen M | 2013 | Duodecim2013，129，14： | 1 |
| 8 | Progestin-releasing:intrauterine systems显示文摘 | Luukkainen T Pakarinen P Toivonen J | 2001 | Semin Reprod Med2001，19，： | 1 |
| 9 | Incidence, aetiology and prognosis of primary subarachnoid haemorhage 显示文摘 | Pakarinen S | 1967 | Acta Neurol Scand1967，43，： | 1 |
| 10 | Minimizing ventricular pacing by a novel atri- oventricular (AV) delay hysteresis algorithm in patients with intact or com- promised intrinsic AV conduction and different atrial and ventricular lead locations显示文摘 | Pakarinen S Toivonen L | 2013 | Ann Meal2013，45，56： | 1 |
| 11 | Endoscopic Surveillance and Primary Prophylaxis Sclerotherapy of Esophageal Varices in Biliary Atresia显示文摘 | Hanna Lampela Silja Kosola Antti Koivusalo Jouni Lauronen Hannu Jalanko Risto Rintala Mikko P. Pakarinen | 2012 | Journal of Pediatric Gastroenterology and Nutrition2012，，5： | 1 |
| 12 | Industrial symbiosiscontributing to more sustainable energy use - an examplefrom the forest industry in Kymenlaakso, Finland显示文摘 | Sokka L Pakarinen S Melanen M | 2011 | Journal of Cleaner Production2011，19，4： | 1 |
| 13 | Functional characterization of the human FSH receptor with an inactivating Ala189Val mutation显示文摘 | Rannikko A Pakarinen P Manna PR | 2003 | Mol Hum Reprod2003，8，4： | 1 |
| 14 | A systems model of training responses and its relationship to hormo- nal responses in elite weight-lifters 显示文摘 | Busso T Hakkinen K Pakarinen A | 1990 | Eur J Appl Physiol1990，61，12： | 1 |
| 15 | Randomized comparison of levonorgstrel and copper releasing intrauterine systems immediately after abortion,with 5 years follow-up显示文摘 | Pakarinen P | 2003 | Contraception2003，68，1： | 1 |
| 16 | Mutation in the follicle stimuLating hormone receptor gene causes hereditary hypergonadotropic ovarian failurel 显示文摘 | Aittomaki K Lucena JL Pakarinen P | 1995 | Cell1995，82，： | 1 |
| 17 | Serum insulin and leptin levels in valproate - associated obesity 显示文摘 | Pylvanen V Knip M Pakarinen A | 2002 | Epilepsia2002，43，： | 1 |
| 18 | Ordered nanostructures written directly by laser interference显示文摘 | Tan C Peng C S Pakarinen J | 2009 | Nanotechnology2009，20，12： | 1 |
| 19 | National centralization of biliary atresia care to an assigned multidisciplinary team provides high-quality outcomes显示文摘 | Hanna Lampela Annukka Ritvanen Silja Kosola Antti Koivusalo Risto Rintala Hannu Jalanko Mikko Pakarinen | 2012 | Scandinavian Journal of Gastroenterology2012，，1： | 1 |
| 20 | Effects of the levonorgestrel-releasing intrauterine system on proliferation and apoptosis in the endometrium显示文摘 | Maruo T Laoag-Fernandez JB Pakarinen P | 2001 | Hum Reprod2001，16，10： | 1 |
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