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| 1 | Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells显示文摘AIM: To study the therapeutic effect of three tubeguides with electrical conductivity associated to mesenchymal stem cells(MSCs) on neuro-muscular regeneration after neurotmesis.METHODS: Rats with 10-mm gap nerve injury were tested using polyvinyl alcohol(PVA), PVA-carbon nanotubes(CNTs) and MSCs, and PVA-polypyrrole(PPy). The regenerated nerves and tibialis anterior muscles were processed for stereological studies after 20 wk. The functional recovery was assessed serially for gait biomechanical analysis, by extensor postural thrust, sciatic functional index and static sciatic functionalindex(SSI), and by withdrawal reflex latency(WRL). In vitro studies included cytocompatibility, flow cytometry, reverse transcriptase polymerase chain reaction and karyotype analysis of the MSCs. Histopathology of lung, liver, kidneys, and regional lymph nodes ensured the biomaterials biocompatibility. RESULTS: SSI remained negative throughout and independently from treatment. Differences between treted groups in the severity of changes in WRL existed, showing a faster regeneration for PVA-CNTs-MSCs(P < 0.05). At toe-off, less acute ankle joint angles were seen for PVA-CNTs-MSCs group(P = 0.051) suggesting improved ankle muscles function during the push off phase of the gait cycle. In PVA-PPy and PVA-CNTs groups, there was a 25% and 42% increase of average fiber area and a 13% and 21% increase of the 'minimal Feret's diameter' respectively. Stereological analysis disclosed a significantly(P < 0.05) increased myelin thickness(M), ratio myelin thickness/axon diameter(M/d) and ratio axon diameter/fiber diameter(d/D; g-ratio) in PVA-CNT-MSCs group(P < 0.05). CONCLUSION: Results revealed that treatment with MSCs and PVA-CNTs tube-guides induced better nerve fiber regeneration. Functional and kinematics analysis revealed positive synergistic effects brought by MSCs and PVA-CNTs. The PVA-CNTs and PVA-PPy are promising scaffolds with electric conductive properties, bio- and cytocompatible that might prevent the secondary neurogenic muscular atrophy by improving the reestablishment of the neuro-muscular junction. | Jorge Ribeiro Tiago Pereira Ana Rita Caseiro Paulo Armada-da-Silva Isabel Pires Justina Prada Irina Amorim Sandra Amado Miguel Franca Carolina Goncalves Maria Ascensao Lopes Jose Domingos Santos Dina Morais Silva Stefano Geuna Ana Lúcia Luís Ana Colette Maurício | 2015 | World Journal of Stem Cells2015，7，6： | 2 |
| 2 | The course of inflammatory bowel disease during pregnancy and postpartum: a prospective European ECCO‐EpiCom Study of 209 pregnant women显示文摘 | N. Pedersen A. Bortoli D. Duricova R. D′Inca M. R. Panelli J. P. Gisbert G. Zoli A. López‐Sanromán F. Castiglione G. Riegler V. Annese P. Gionchetti A. Prada E. D. Pont A. Timmer C. Felley M. Shuhaibar E. V. Tsianos C. Dejaco F. J. Baert T. Jess M. Lebech | 2013 | Aliment Pharmacol Ther2013，，5： | 2 |
| 3 | ,Voltage stability and thermal limit constraints on the maximum loading of electrical energy distribution feeders 显示文摘 | PRADA R B SOUZA L J | 1998 | IEE Pro-Gen Trans & Distr1998，145，5： | 1 |
| 4 | Diaphyseal chondroblastoma in a long bone: first report显示文摘 | D. Azorín I. González-Mediero I. Colmenero I. Prada F. López-Barea | 2006 | Skeletal Radiology2006，，1： | 1 |
| 5 | The iterative time reveral process: analysis of the convergence显示文摘 | PRADA C THOMAS J L FINK M | 1995 | J Acoust Soc Am1995，97，1： | 1 |
| 6 | Behavior in simulated body fluid of calcium phosphate coatings obtained by laser ablation显示文摘 | CLERIES L Femendez Pradas J M | 2000 | Biomaterials2000，21，18： | 1 |
| 7 | Evaluation of the use of different hydrocarbon fuels for gas reburning显示文摘 | MILLERA A ALZUETA M U PRADA L | 1997 | Fuel1997，76，1415： | 1 |
| 8 | The iterative time re- versal process : Analysis of the convergence 显示文摘 | PRADA C THOMAS J L FINK M | 1995 | Journal of the Acoustical Society of America1995，97，1： | 1 |
| 9 | Antimony as tracer of the anthropogenic influence on soils and estuarine sediments 显示文摘 | Cal-Prieto M J Carlosena A Andrade J M Martinez M L Muniategui S Lopez-Mahia P Prada D | 2001 | Water Air Soil Pollut2001，129，14： | 1 |
| 10 | Behavior in simulated body fluid of calcium phosphatc coatings obtained by laser ablation 显示文摘 | Cleries L Pradas J M | 2000 | Biomaterials2000，21，18： | 1 |
| 11 | Reducing avai- lable soluble β-amyloid prevents progression of cerebral amyloid angiopathy in transgenic mice 显示文摘 | Gregory J L Prada C M Fine S J | 2012 | Neuropathol Exp Neurol2012，71，11： | 1 |
| 12 | Sorting of phaseolin to the vacuole is saturable and requires a short C-terminal peptide显示文摘 | Frigerio L de Virgilio M Prada A | 1998 | Plant Cell1998，10，： | 1 |
| 13 | Behavior in simulated body fluid of calcium phosphate coatings obtained by laser ablation 显示文摘 | Femendez Pradas J M | 2000 | Biomater2000，21，18： | 1 |
| 14 | Idenfication of Echino- coccus geanulosus microRNAs and their expression in different life cycle stages and parasite genotypes显示文摘 | Cucher M Prada L Mourglia-Ettlin G | 2011 | Int J Parasitol2011，41，34： | 1 |
| 15 | Behavior in simulated body fluid of calcium phosphate coatings obtained by laser ablation 显示文摘 | Cleries L Femendez Pradas J M | 2000 | Biomaterials2000，21，18： | 1 |
| 16 | Influence of the nature of the porous confining network on the sorption,diffusion and mechanical properties of hydrogel IPNs显示文摘 | Gallego Ferrer Monleón Pradas M Gómez Ribelles J L | | 0，，46： | 1 |
| 17 | Sorting of phaseolin to the vacuole is saturable and requires a short C-terminal peptide显示文摘 | Frigerio L Virgilio M Prada A | 1998 | Plant Cell1998，10，6： | 1 |
| 18 | Three-dimensional echocardiography:in vivo validation for right ventricular free wall mass as an index of hypertrophy显示文摘 | Jiang L Vazquez de Prada JA Handschumacher MD | 1994 | J Am Coll Cardiol1994，23，7： | 1 |
| 19 | Hydroprocessing of crude palm oil at pilot plant scale显示文摘 | Guzman A Torres J E Prada L P | 2010 | Catalysis Today2010，156，12： | 1 |
| 20 | Endothelial dysfunction and preeclampsia:role of oxidative stress显示文摘 | S魣NCHEZ-ARANGUREN L C PRADA C E RIAO-MEDINA C E | 2014 | Frontiers in Physiology2014，5，： | 1 |
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