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| 1 | 食物过敏的定义、流行性、诊断及治疗显示文摘食物过敏是异质性群体相关疾病,会通过一种或多种方式对多个器官造成影响。其反应的严重程度可从轻度的局部反应到全身性的过敏反应。从机制上来看,食物过敏是Th2细胞驱动的免疫紊乱疾病,食物特异性IgE是这种速发型不良反应的基础。过敏的部位和症状并不一定相同。食物过敏常会与自身免疫性(如乳糜泻)和非免疫性(如乳糖不耐受)的其他食物不良反应相混淆。为了正确诊断食物过敏,需要详细了解患者病史,检测特异性IgE,并进行经口激发试验来确诊。一些辅助因素(如运动、药物和酒精)有助于诱发食物过敏,会进一步增加诊断的复杂性。以食物提取物为基础的诊断试验并不能反映症状的严重程度,而检测单个食物致敏原IgE的新一代分子诊断技术,则能够为临床医生和患者提供可靠的症状严重程度依据。分子诊断还有助于确定食物过敏是直接源于对食物的暴露还是间接源于花粉过敏(交叉反应)。流行病学调查表明,桃过敏在欧洲主要是源于对桃的食用,而在中国则是源于对艾蒿花粉的初级致敏。但这两种情况都是由来自同一家族的致敏原分子介导的。流行病学调查深入揭示了食物过敏的病因、流行性及其所包含的风险因素,为预防食物过敏提供了循证策略。过去的十年中,食物过敏在发达国家盛行。经济发展和城市化进程都被认为会导致食物过敏流行性的增加,而且饮食习惯不同,过敏的食物也不同。目前,分子变态反应学和生物技术正在开发安全的免疫治疗方法,有望能够减轻日益加重的食物过敏负担。一种使用低致敏性突变重组分子的免疫疗法已经开始进行第一阶段临床试验的评估。食物致敏原名单的不断完善为转基因食品致敏性的评估提供了坚实基础。 | Ronald van Ree Lars K. Poulsen Gary WK Wong Barbara K. Ballmer-Weber 高中山 贾旭东 | 2015 | 中华预防医学杂志2015，49，1： | 28 |
| 2 | Signaling transduction pathways involved in basophil adhesion and histamine release显示文摘Background Little is known about basophil with respect to the different signaling transduction pathways involved in spontaneous, cytokine or anti-IgE induced adhesion and how this compares to IgE-dependent and IgE-independent mediator secretion. The purpose of the present study was to investigate the roles of β1 andβ2 integrins in basophil adhesion as well as hosphatidylinositol 3-kinase (PI3K), src-kinases and extracellular signal regulated kinase (ERK)1/2 in basophil adhesion and histamine release (HR). Methods Basophils (purity of 10%-50%) were preincubated with anti-CD29 or anti-CD18 blocking antibodies before used for adhesion study. Basophils were preincubated with the pharmacological inhibitors wortmannin, PP1, PD98059 before used for adhesion and HR study. Cell adherence to bovine serum albumin (BSA) or fibronectin (Fn) was monitored using cell associated histamine as a basophil marker and the histamine was measured by the glass fiber assay. Results Basophil spontaneous adhesion to Fn was inhibited by anti-CD29. Interleukin (IL)-3, granulocyte/macrophage colony stimulating factor (GM-CSF) induced adhesion to BSA was inhibited by anti-CD18. Wortmannin at 1 μmol/L and PP1 at 20 μmol/L strongly interfered with, whereas PD98059 at 50 μmol/L weakly inhibited basophil spontaneous adhesion to Fn. One μmol/L wortmannin strongly inhibited IL-3, IL-5, GM-CSF and anti-IgE induced adhesion to BSA. PP1 at 20 μmol/L partly inhibited anti-IgE induced adhesion. Fifty μmol/L PD98059 marginally inhibited IL-5, weakly inhibited anti-IgE, partly inhibited GM-CSF induced adhesion. Wortmannin, PP1 and PD98059 inhibited anti-IgE (1:100 or 1:1000) induced basophil HR in a dose dependent manner. They inhibited calcium ionophore A23187 (10 μmol/L, 5 μmol/L) induced basophil HR in a dose dependent manner, but to different extend with PP1 being the most efficient. Conclusions Basophil spontaneous adhesion to Fn is mediated by β1-integrins whereas cytokine induced adhesion to BSA is mediated by β2-integrins. PI3K, src-kinases and ERK1/2 play distinct signaling roles in basophil adhesion and HR. PI3K is the key player whileERK1/2 is the weakest participant. | SHA Quan Lars K. Poulsen Jens Gerwien NielsФdum Per Stahl Skov | 2006 | Chinese Medical Journal2006，，2： | 8 |
| 3 | Investigation of overburden behaviour for grout injection to control mine subsidence显示文摘This paper describes a field and numerical investigation of the overburden strata response to underground longwall mining, focusing on overburden strata movements and stress concentrations.Subsidence related high stress concentrations are believed to have caused damage to river beds in the Illawarra region, Australia. In the field study, extensometers, stressmeters and piezometers were installed in the overburden strata of a longwall panel at West Cliff Colliery. During longwall mining, a total of1000 mm tensile deformation was recorded in the overburden strata and as a result bed separation and gaps were formed. Bed separation was observed to start in the roof of the mining seam and gradually propagate toward the surface as the longwall face advanced. A substantial increase in the near-surface horizontal stresses was recorded before the longwall face reached the monitored locations. The stresses continued to increase as mining advanced and they reached a peak at about 200 m behind the longwall face. A numerical modelling study identified that the angle of breakage(i.e., the angle of the boundary of caved zone) behind the longwall face and over the goaf was 22–25° from vertical direction. This is consistent with the monitoring results showing the high gradient of stresses and strains on the surface150–320 m behind the mining face. | Shen Baotang Poulsen Brett | 2014 | International Journal of Mining Science and Technology2014，24，3： | 8 |
| 4 | Pain and chronic pancreatitis: A complex interplay of multiple mechanisms显示文摘Despite multiple theories on the pathogenesis of pain in chronic pancreatitis,no uniform and consistently successful treatment strategy exists and abdominal pain still remains the dominating symptom for most patients and a major challenge for clinicians.Traditional theories focussed on a mechanical cause of pain related to anatomical changes and evidence of increased ductal and interstitial pressures.These observations form the basis for surgical and endoscopic drainage procedures,but the outcome is variable and often unsatisfactory.This underscores the fact that other factors must contribute to pathogenesis of pain,and has shifted the focus towards a more complex neurobiological understanding of pain generation.Amongst other explanations for pain,experimental and human studies have provided evidence that pain perception at the peripheral level and central pain processing of the nociceptive information is altered in patients with chronic pancreatitis,and resembles that seen in neuropathic and chronic pain disorders.However,pain due to e.g.,complications to the disease and adverse effects to treatment must not be overlooked as an additional source of pain.This review outlines the current theories on pain generation in chronic pancreatitis which is crucial in order to understand the complexity and limitations of current therapeutic approaches.Furthermore,it may also serve as an inspiration for further research and development of methods that can evaluate the relative contribution and interplay of different pain mechanisms in the individual patients,before they are subjected to more or less empirical treatment. | Jakob Lykke Poulsen Sφren Schou Olesen Lasse Paludan Malver Jens Brφndum Frφkjr Asbjφrn Mohr Drewes | 2013 | World Journal of Gastroenterology2013，19，42： | 5 |
| 5 | Histological grading and staging of chronic hepatitis显示文摘 | Kamal Ishak Amelia Baptista Leonardo Bianchi Francesco Callea Jan De Groote Fred Gudat Helmut Denk Valeer Desmet Gerhard Korb Roderick N.M. MacSween M.James Phillips Bernard G. Portmann Hemming Poulsen Peter J. Scheuer Martin Schmid Heribert Thaler | 1995 | Journal of Hepatology1995，，6： | 4 |
| 6 | Histological grading and staging of chronic hepatitis显示文摘 | Kamal Ishak Amelia Baptista Leonardo Bianchi Francesco Callea Jan De Groote Fred Gudat Helmut Denk Valeer Desmet Gerhard Korb Roderick N.M. MacSween M.James Phillips Bernard G. Portmann Hemming Poulsen Peter J. Scheuer Martin Schmid Heribert Thaler | 1995 | Journal of Hepatology1995，，6： | 4 |
| 7 | Indocyanine green plasma disappearance rate as dynamic liver function test in critically ill patients显示文摘 | B. M. HALLE T. D. POULSEN H. P. PEDERSEN | 2014 | Acta Anaesthesiol Scand2014，，10： | 2 |
| 8 | Quantitation of minimal residual disease in multiple myeloma using allele-specific real-time PCR assay 显示文摘 | Rasmussen T Poulsen TS Honore L | 2000 | Exp Hematol2000，28，： | 2 |
| 9 | PNAS:科学家首次鉴别出让寨卡病毒如此致命的关键蛋白显示文摘当寨卡病毒横空出世时科学家们对其知之甚少。在过去一年里研究者们通过深入研究来阐明寨卡病毒为何会引发一系列危险的健康问题。包括新生儿缺陷等,比如小头畸型和神经性问题(格--巴二氏综合征等)。但研究者们并不知道寨卡病毒哪种蛋白会让其变得具有杀伤性。当然他们也不清楚这些蛋白质的作用机理是什么。 | Ge Li Melissa Poulsen Csaba Fenyvuesvolgyi Yoko Yashiroda Minoru Yoshida J. Marc Simard Robert C. Gallo Richard Y. Zhao | 2017 | 现代生物医学进展2017，17，3： | 2 |
| 10 | A generalized autocovariance least - squares method for Kalmanfilter tuning显示文摘 | AKESSON B M JORGENSEN J B POULSEN N K | 2008 | Journal of Process Control2008，18，78： | 1 |
| 11 | Prognostic factors in osteosarcomas显示文摘 | Bentzen SM Poulsen HS Kaae S | 1988 | Cancer1988，62，： | 1 |
| 12 | A luminescent oxygen channeling immuno- assay for the determination of insulin in human plasma显示文摘 | Poulsen F Jensen KB | 2007 | Biomol Screen2007，12，2： | 1 |
| 13 | Role of oxidative DNA damage in cancer initiation and promotion显示文摘 | Poulsen H E Preme H Loft S | 1998 | Eur J Cancer Preview1998，7，1： | 1 |
| 14 | Pseudonormal and restrictive filling patterns predict left ventricular dilation and cardiac death after a first myocardial infarction: a serial color M-mode Doppler echocardiographic study 显示文摘 | Moller J E Sondergaard E Poulsen S H | 2000 | J Am Coll Cardiol2000，36，6： | 1 |
| 15 | Allergen cross - reactivity between house - dust mites and other invertebrates显示文摘 | SIDENIUS K E HALLAS T E POULSEN L K | 2001 | Allergy2001，56，8： | 1 |
| 16 | Limit state analysis of reinforced concrete plates subjected to in-plane forces 显示文摘 | Poulsen P N Damkilde L | 2000 | International Journal of Solids and StrucRtres2000，37，42： | 1 |
| 17 | IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th 1 and Th2 Currures显示文摘 | Blom L Poulsen LK | 2012 | J Immunol2012，189，9： | 1 |
| 18 | In vitro Thl and Th2 cell polarization is severely influenced by the initial ratio of naive and memory CD4+Tcells显示文摘 | Blom L Poulsen LK | 2013 | J ImmunolMethods2013，397，8： | 1 |
| 19 | Cellular radiosensitivity of small-cell lung cancer cell lines显示文摘 | Krarup M Poulsen HS Spang-Thomsen M | | 0，，01： | 1 |
| 20 | A Generalized Autocovariance Least-square Method for Kalman Filter Tuning显示文摘 | AKESSON B M JORGENSEN J B POULSEN N K | | 0，，： | 1 |
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