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| 1 | No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil显示文摘AIM:To investigate the association between human papillomavirus(HPV)and esophageal squamous cell carcinoma(ESCC)in southern Brazil.METHODS:We studied 189 esophageal samples from125 patients from three different groups:(1)102 biopsies from 51 patients with ESCC,with one sample from the tumor and another from normal esophageal mucosa distant from the tumor;(2)50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma(HNSCC);and(3)37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients,not exposed to smoking or alcohol consumption.Nested-polymerase chain reaction(PCR)with the MY09/11 and GP5/6 L1primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin blocks.All PCR reactions were performed with a positive control(cervicovaginal samples),with a negative control(Human Genomic DNA)and with a blank reaction containing all reagents except DNA.We took extreme care to prevent DNA contamination in sample collection,processing,and testing.RESULTS:The histological biopsies confirmed the diagnosis of ESCC in 52 samples(51 from ESCC group and 1 from the HNSCC group)and classified as well differentiated(12/52,23.1%),moderately differentiated(27/52,51.9%)or poorly differentiated(7/52,13.5%).One hundred twenty-eight esophageal biopsies were considered normal(51 from the ESCC group,42 from the HNSCC group and 35 from dyspeptic patients).Nine had esophagitis(7 from the HNSCC and 2 from dyspeptic patients).Of a total of 189 samples,only 6 samples had insufficient material for PCR analysis:1 from mucosa distant from the tumor in a patient with ESCC,3from patients with HNSCC and 2 from patients without cancer.In 183 samples(96.8%)GAPDH,G3PDH and/orβ-globin were amplified,thus indicating the adequacy of the DNA in those samples.HPV DNA was negative in all the 183 samples tested:52 with ESCC,9 with esophagitis and 122 with normal esophageal mucosa.CONCLUSION:There was no evidence of HPV infection in different ESCC from southern Brazil. | Luís Carlos Moreira Antunes Joo Carlos Prolla Antonio de Barros Lopes Marta Pires da Rocha Renato Borges Fagundes | 2013 | World Journal of Gastroenterology2013，19，39： | 4 |
| 2 | Circulating leptin and its muscle gene expression in Nellore cattle with divergent feed efficiency显示文摘Background: Leptin has a strong relation to important traits in animal production, such as carcass composition,feed intake, and reproduction. It is mainly produced by adipose cells and acts predominantly in the hypothalamus.In this study, circulating leptin and its gene expression in muscle were evaluated in two groups of young Nellore bulls with divergent feed efficiency. Individual dry matter intake(DMI) and average daily gain(ADG) of 98 Nellore bulls were evaluated in feedlot for 70 d to determinate the residual feed intake(RFI) and select 20 animals for the high feed efficient(LRFI) and 20 for the low feed efficient(HRFI) groups. Blood samples were collected on d 56 and at slaughter(80 d) to determine circulating plasma leptin. Samples of Longissimus dorsi were taken at slaughter for leptin gene expression levels.Results: DMI and RFI were different between groups and LRFI animals showed less back fat and rump fat thickness,as well as less pelvic and kidney fat weight. Circulating leptin increased over time in all animals. Plasma leptin was greater in LRFI on 56 d and at slaughter(P = 0.0049). Gene expression of leptin were greater in LRFI animals(P = 0.0022) in accordance with the plasma levels. The animals of the LRFI group were leaner, ate less, and had more circulating leptin and its gene expression.Conclusion: These findings demonstrated that leptin plays its physiological role in young Nellore bulls, probably controlling food intake because feed efficient animals have more leptin and lower residual feed intake. | Lúcio Flávio Macedo Mota Cristina Moreira Bonafé Pamela Almeida Alexandre Miguel Henrique Santana Francisco JoséNovais Erika Toriyama Aldrin Vieira Pires Saulo da Luz Silva Paulo Roberto Leme JoséBento Sterman Ferraz Heidge Fukumasu | 2018 | Journal of Animal Science and Biotechnology2018，9，1： | 3 |
| 3 | GO FAIR Brazil:A Challenge for Brazilian Data Science显示文摘The FAIR principles,an acronym for Findable,Accessible,Interoperable and Reusable,are recognised worldwide as key elements for good practice in all data management processes.To understand how the Brazilian scientific community is adhering to these principles,this article reports Brazilian adherence to the GO FAIR initiative through the creation of the GO FAIR Brazil Office and the manner in which they create their implementation networks.To contextualise this understanding,we provide a brief presentation of open data policies in Brazilian research and government,and finally,we describe a model that has been adopted for the GO FAIR Brazil implementation networks.The Brazilian Institute of Information in Science and Technology is responsible for the GO FAIR Brazil Office,which operates in all fields of knowledge and supports thematic implementation networks.Today,GO FAIR Brazil-Health is the first active implementation network in operation,which works in all health domains,serving as a model for other fields like agriculture,nuclear energy,and digital humanities,which are in the process of adherence negotiation.This report demonstrates the strong interest and effort from the Brazilian scientific communities in implementing the FAIR principles in their research data management practices. | Luana Sales Patricia Henning Viviane Veiga Maira Murrieta Costa Luis Fernando Sayao Luiz Olavo Bonino da Silva Santos Luis Ferreira Pires | 2020 | Data Intelligence2020，2，1： | 3 |
| 4 | Object-oriented and distributed approach for programming robotic manufacturing cells 显示文摘 | Sda Costa | 2000 | Robotics and Computer-Integrated Manufacturing2000，16，1： | 1 |
| 5 | Ectopic breast cancer in the anterior chest wall:a case report and literature review显示文摘 | da Silva BB dos Santos AR Pires CG | 2008 | Eur J Gynaecol Oncol2008，29，6： | 1 |
| 6 | Mel-CAM (CD146) expression in parotid mucoepidermoid carcinoma 显示文摘 | Pires FR Shih le M da Cruz Perez DE | 2003 | Oral Oncol2003，39，3： | 1 |
| 7 | A comparative study of titanium nitrides, TiN, TiNbN and TiCN, as coatings for biomedical applications显示文摘 | A.P. Serro C. Completo R. Cola?o F. dos Santos C. Lobato da Silva J.M.S. Cabral H. Araújo E. Pires B. Saramago | 2009 | Surface & Coatings Technology2009，，24： | 1 |
| 8 | Salivary gland tumors in children and adolescents: a clinicopathologic and immunohistochemical study of fiftythree cases显示文摘 | da Cruz Perez DE Pires F R Alves F A | | Int J Pediatr Otorhinolaryngol 200 10，68，7： | 1 |
| 9 | Evaluation of low-level laser therapy of osteoblastic cells显示文摘 | Pires Oliveira DA de Oliveira RF Zangaro RA | 2008 | Photomed Laser Surg2008，26，40： | 1 |
| 10 | IFN-gamma limits Th9-mediated autoimmune inflammation through dendritic cell / modulation of IL-27 显示文摘 | MURUGAIYAN G BEYNON V PIRES DA CUNHA A | 2012 | J Immunol2012，189，11： | 1 |
| 11 | Institutional h - index: the performance of a new metric in the evaluation of Brazilian psychiatric post - graduation programs 显示文摘 | Mariana Pires Da Luz Carla Marques -Portella Mauro Mendlowicz | 2008 | Scientometrics2008，77，2： | 1 |
| 12 | The triple helix model as a motor for the creative use of telematics显示文摘 | Eduardo Anselmo de Castro Carlos Rodrigues Carlos Esteves Artur da Rosa Pires | 2000 | Research Policy2000，29，： | 1 |
| 13 | Ectopiv breastcancer in the anterior chestwal:l a case report and literature review 显示文摘 | da Silva BB dosSantos AR Pires CG | 2008 | Eur J Gynaecol Oncol2008，29，6： | 1 |
| 14 | An efficient methodology for coherency-based dynamic equivalents显示文摘 | Pires de Souza E J S Leite da Silva A M | 1992 | IEE Proceedings- Generation Transmission and Distribution1992，139，5： | 1 |
| 15 | Chemokines and mitochondrial products activate neutrophils to amplify organ injury during mouse acute liver failure 显示文摘 | Marques PE Amaral SS Pires DA | 2012 | Hepatology2012，56，： | 1 |
| 16 | Photodynamic therapy for pythiosis显示文摘 | Layla Pires Sandra de M. G. Bosco Nelson F. da Silva Junior Cristina Kurachi | 2013 | Veterinary Dermatology2013，，1： | 1 |
| 17 | Linking process capability analysis and least limiting water range for assessing soil physical quality显示文摘 | Alvaro Pires da Silva B.D. Kay | | Soil & Tillage Research0，，： | 1 |
| 18 | Sialometry: aspects of clinical interest显示文摘 | Falco DP da Mota LM Pires AL | 2013 | Rev Bras Reumatol2013，53，6： | 1 |
| 19 | Expression of genes controlling fat deposition in two genetically diverse beef cattle breeds fed high or low silage diets显示文摘 | Costa A S H da Pires V M R Fontes C M G A et a1 | 2013 | BMC Veterinary Research2013，，9： | 1 |
| 20 | The influence of gelation rate on the physical properties/structure of salt-induced gels of soy protein isolate - gellan gum 显示文摘 | Pires Vilela J A Cavallieri L F Lopes Da Cunha R | 2011 | Food Hydrocolloids2011，25，7： | 1 |
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