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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | Transcriptome profile of human neuroblastoma cells in the hypomagnetic field显示文摘Research has shown that the hypomagnetic field(HMF)can affect embryo development,cell proliferation,learning and memory,and in vitro tubulin assembly.In the present study,we aimed to elucidate the molecular mechanism by which the HMF exerts its effect,by comparing the transcriptome profiles of human neuroblastoma cells exposed to either the HMF or the geomagnetic field.A total of 2464 differentially expressed genes(DEGs)were identified,216 of which were up-regulated and2248 of which were down-regulated after exposure to the HMF.These DEGs were found to be significantly clustered into several key processes,namely macromolecule localization,protein transport,RNA processing,and brain function.Seventeen DEGs were verified by real-time quantitative PCR,and the expression levels of nine of these DEGs were measured every 6 h.Most notably,MAPK1 and CRY2,showed significant up-and down-regulation,respectively,during the first 6 h of HMF exposure,which suggests involvement of the MAPK pathway and cryptochrome in the early bio-HMF response.Our results provide insights into the molecular mechanisms underlying the observed biological effects of the HMF. | MO WeiChuan LIU Ying BARTLETT Perry F HE RongQiao | 2014 | Science China(Life Sciences)2014，57，4： | 8 |
| 3 | Introduction to the thematic issue “From brain function to therapy”显示文摘This thematic issue of Science China Life Sciences,'From brain function to therapy',highlights some of the exciting research being undertaken in the Joint Laboratory of Neuroscience and Cognition,an international scientific collaboration between the Queensland Brain Institute(QBI)at | BARTLETT Perry F HE RongQiao | 2014 | Science China(Life Sciences)2014，57，4： | 7 |
| 4 | The role of the N-methyl-D-aspartate receptor in the proliferation of adult hippocampal neural stem and precursor cells显示文摘New neurons are continuously generated from resident pools of neural stem and precursor cells(NSPCs)in the adult brain.There are multiple pathways through which adult neurogenesis is regulated,and here we review the role of the N-methyl-D-aspartate receptor(NMDAR)in regulating the proliferation of NSPCs in the adult hippocampus.Hippocampal-dependent learning tasks,enriched environments,running,and activity-dependent synaptic plasticity,all potently up-regulate hippocampal NSPC proliferation.We first consider the requirement of the NMDAR in activity-dependent synaptic plasticity,and the role the induction of synaptic plasticity has in regulating NSPCs and newborn neurons.We address how specific NMDAR agonists and antagonists modulate proliferation,both in vivo and in vitro,and then review the evidence supporting the hypothesis that NMDARs are present on NSPCs.We believe it is important to understand the mechanisms underlying the activation of adult neurogenesis,given the potential that endogenous stem cell populations have for repopulating the hippocampus with functional new neurons.In conditions such as age-related memory decline,neurodegeneration and psychiatric disease,mature neurons are lost or become defective;as such,stimulating adult neurogenesis may provide a therapeutic strategy to overcome these conditions. | TAYLOR Chanel J HE RongQiao BARTLETT Perry F | 2014 | Science China(Life Sciences)2014，57，4： | 6 |
| 5 | Mechanisms of biliary carcinogenesis and growth显示文摘Cholangiocarcinoma is a rare cancer originating from the neoplastic transformation of the epithelial cells (i.e. cholangiocytes) that line the biliary tract. The prognosis for patients with cholangiocarcinoma is grim due to lack of viable treatment options. The increase in world-wide incidence and mortality from cholangiocarcinoma highlights the importance of understanding the intracellular mechanisms that trigger the neoplastic transformation of cholangiocytes and the growth of biliary cancers. The purpose of the following review is to address what has been learned over the past decade concerning the molecular basis of cholangiocarcinogenesis. The material presented is divided into two sections:(1) mechanisms regulating neoplastic transformation of cholangiocytes; and (2) factors regulating cholangiocarcinoma growth. An understanding of the growth regulatory mechanisms of cholangiocarcinoma will lead to the identification of therapeutic targets for this devastating cancer. | Candace Wise Metaneeya Pilanthananond Benjamin F Perry Gianfranco Alpini Michael McNeal Shannon S Glaser | 2008 | World Journal of Gastroenterology2008，14，19： | 5 |
| 6 | Mice cloned from embryonic stem cell显示文摘 | Wakayama T Rodriguez I Perry A C F | 1999 | Proc Natl Acad Sci USA1999，，14： | 1 |
| 7 | Long-term outcome afterantiviral therapy of patients with hepatitis C virusinfection and de-compensated cirrhosis显示文摘 | Iacobellis A Perri F Valvano MR | 2011 | Clin Gastroenterol Hepatol2011，9，： | 1 |
| 8 | Guidelines for treat- ment of acute pancreatitis显示文摘 | Andriulli A Perri F Annese V | 1999 | Gut1999，44，4： | 1 |
| 9 | Guidelines for treatment of acute pancreatitis显示文摘 | ANDRULLI A PERRI F ANNESE V | 1999 | Gut1999，44，4： | 1 |
| 10 | Intraduodenal lipase activity in celiac disease assessed by means of ^13 C mixed triglyceride breath test显示文摘 | Perri F Pastore M Festa V | 1998 | J Pediatr Gastroenterol Nutr1998，7，4： | 1 |
| 11 | Guidelines for treatment of acute pancreatitis 显示文摘 | Andrulli A Perri F Annese V | 1999 | Gut1999，44，4： | 1 |
| 12 | Determination of the Sum of bilirubin sugar conjugates in plasma by bilirubin oxidase显示文摘 | Doumas BT Vein F Perry B | | Clinical Chemistry0，，： | 1 |
| 13 | Flowfield produced by trailing vortices in the vicinity of the ground显示文摘 | HARVEY J K PERRY F J | 1971 | AIAA Journal1971，9，8： | 1 |
| 14 | Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study 显示文摘 | Kumarasamy KK Toleman MA Walsh TR Bagaria J Butt F Balakrishnan R Chaudhary U Doumith M Giske CG Irfan S Krishnan P Kumar AV Maharjan S Mushtaq S Noorie T Paterson DL Pearson A Perry C Pike R Rao B Ray U Sarma JB Sharma M Sheridan E Thirunarayan MA Turton J Upadhyay S Warner M Welfare W Livermore DM Woodford N | 2010 | Lancet Infect Dis2010，10，9： | 1 |
| 15 | Th1/Th2 cytomet- rie bead array can discriminate cytokine secretion from endogenously activated cells in pulmonary disease, re- cent and remote infection in tuberculosis显示文摘 | Talat N Shahid F Perry S | 2011 | Cytokine2011，54，2： | 1 |
| 16 | Crystal structures of the ribosome in complex with release factors RFI and RF2 Bound to a cognate stop codon 显示文摘 | Perry S Brodersen D E Murphy F V 4th | 2005 | Cell2005，123，7： | 1 |
| 17 | Determination of the of bilirubin sugar conjugates in plasma by bilirubin oxidase显示文摘 | Domuas B T Yein F Perry B | 1999 | Chin Chem1999，45，8： | 1 |
| 18 | Microbial primary dolomite from a Norian carbonate plalform: Northern Cala bria, southern Ilaly显示文摘 | Mastandrea A Perri E Russo F | 2006 | Sedimentology2006，53，： | 1 |
| 19 | Determination of the sum of bilirubin sugar conjugates in plasma by bilirubin oxidase 显示文摘 | Doumas B T Yein F Perry B | 1999 | Clin Chem1999，45，8： | 1 |
| 20 | Contextual Factors as Moderators of the Effect of Employee Ethical Ideology on Ethical Decision-making 显示文摘 | Callanan G A Rotenberry P F Perri D F | 2010 | International Journal of Management2010，27，1： | 1 |
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