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| 1 | Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters显示文摘The efficacy of the CRISPR/Cas9 system in grapevine(Vitis vinifera L.)has been documented,but the optimization of this system,as well as CRISPR/Cas9-mediated multiplex genome editing,has not been explored in this species.Herein,we identified four VvU3 and VvU6 promoters and two ubiquitin(UBQ)promoters in grapevine and demonstrated that the use of the identified VvU3/U6 and UBQ2 promoters could significantly increase the editing efficiency in grape by improving the expression of sgRNA and Cas9,respectively.Furthermore,we conducted multiplex genome editing using the optimized CRISPR/Cas9 vector that contained the conventional multiple sgRNA expression cassettes or the polycistronic tRNA-sgRNA cassette(PTG)by targeting the sugar-related tonoplastic monosaccharide transporter(TMT)family members TMT1 and TMT2,and the overall editing efficiencies were higher than 10%.The simultaneous editing of TMT1 and TMT2 resulted in reduced sugar levels,which indicated the role of these two genes in sugar accumulation in grapes.Moreover,the activities of the VvU3,VvU6,and UBQ2 promoters in tobacco genome editing were demonstrated by editing the phytoene desaturase(PDS)gene in Nicotiana benthamiana leaves.Our study provides materials for the optimization of the CRISPR/Cas9 system.To our knowledge,our simultaneous editing of the grape TMT family genes TMT1 and TMT2 constitutes the first example of multiplex genome editing in grape.The multiplex editing systems described in this manuscript expand the toolbox of grape genome editing,which would facilitate basic research and molecular breeding in grapevine. | Chong Ren Yanfei Liu Yuchen Guo Wei Duan Peige Fan Shaohua Li Zhenchang Liang | 2021 | Horticulture Research2021，8，1： | 6 |
| 2 | A key‘foxy’aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape‘Concord’显示文摘‘Concord’,the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L.,possesses a special‘foxy’aroma predominantly resulted from the accumulation of methyl anthranilate(MA)in berries.This aroma,however,is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V.vinifera.Here we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key‘foxy’aroma gene,anthraniloyl-CoA:methanol acyltransferase(AMAT),that is responsible for MA biosynthesis.We found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in‘Concord’and other V.labrusca-derived grapes.These promoter variants,all with direct and inverted repeats,were further confirmed in more than 1,300 Vitis germplasm.Moreover,functional impact of these indels was validated in transgenic Arabidopsis.Superimposed on the promoter regulation,large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some V.vinifera grapes.Elucidation of the AMAT genetic regulation advances our understanding of the‘foxy’aroma trait and makes it genetically trackable and amenable in grapevine breeding. | Yingzhen Yang JoséCuenca Nian Wang Zhenchang Liang Honghe Sun Benjamin Gutierrez Xiaojun Xi Jie Arro YiWang Peige Fan Jason Londo Peter Cousins Shaohua Li Zhangjun Fei Gan-Yuan Zhong | 2020 | Horticulture Research2020，7，1： | 3 |
| 3 | Facile preparation of nano-g-C_(3)N_(4)/UiO-66-NH_(2) composite as sorbent for high-efficient extraction and preconcentration of food colorants prior to HPLC analysis显示文摘In this work,the nano-g-C_(3)N_(4)/Ui O-66-NH_(2)composite was prepared by one-step solvothermal method.The as-prepared composite was characterized by scanning electron microscopy,Brunner-Emmet-Teller measurement,energy dispersive spectrometer,X-ray diffraction,and Fourier transform infrared spectroscopy.By using nano-g-C_(3)N_(4)/Ui O-66-NH_(2)composite as sorbent,a dispersive solid-phase extraction coupled with high-performance liquid chromatography was developed to sensitive analysis of food colorants including tartrazine,amaranth,carmine,sunset yellow,allura red and bright blue.The experiment parameters including the amount of sorbent,adsorption time,the p H of adsorption solution,desorption time,desorption solvent,the p H of desorption solution as well as the proportion between desorption solvent and buffer solvent were investigated.Under the optimized conditions,the limits of detection(S/N=3) and limits of quantitation (S/N=10) were determined in the ranges of 0.08-0.8 and 0.2-2.0 ng/m L,respectively.With the developed sample pretreatment method,carmine and brilliant blue were determined from blueberry juice by HPLC-DAD.The contents were calculated as 1.53μg/m L and0.17μg/mL,respectively. | Xiaowan Zhang Yixin Yang Peige Qin Lizhen Han Wenli Zhu Shaofeng Duan Minghua Lu Zongwei Cai | 2022 | Chinese Chemical Letters2022，33，2： | 2 |
| 4 | Sugar and acid concentrations in 98 grape eultivars analyzed by principal component analysis 显示文摘 | Liu Huaifeng Wu Benhong Fan Peige | 2006 | Journal of the Science of Food and Agriculture2006，86，10： | 1 |
| 5 | Invitroactivityofminocyclinealoneand in combination with cefoperazone - sulbactam againstcarbapenem-resistantAcinetobacterbaumannii显示文摘 | PEIG MAOY SUNY | 2012 | MicrobDrugResist2012，18，6： | 1 |
| 6 | Genome-wide profiling of histone H3 lysine 27 trimethylation and its modification in response to chilling stress in grapevine leaves显示文摘Histone H3 lysine 27 trimethylation(H3K27me3) is a histone modification associated with transcriptional repression. However, insights into the genome-wide pattern of H3K27me3 in grapevines are limited. Here, anti-H3K27 chromatin immunoprecipitation(ChIP), high-throughput sequencing, and transcriptome analysis were performed using leaves of Vitis amurensis. The leaves were treated at 4°C for 2 h and 24 h and used to investigate changes in H3K27me3 under chilling treatment. The results show that H3K27me3 is well-distributed both in gene regions(-50%) and in the intergenic region(-50%) in the grapevine genome(Vitis vinifera ‘Pinot Noir PN40024'). H3K27me3 was found to be localized in8 368 annotated gene regions in all detected samples(leaves at normal temperature and under chilling treatments) and mainly enriched in gene bodies with the adjacent promoter and downstream areas. The short-term chilling treatments(4°C for 2 h) induced 2 793 gains and 305losses in H3K27me3 modification. Subsequently, 97.3% of the alterations were restored to original levels after 24 h treatment. The ChIP-qPCR for five differential peaks showed similar results to the data for ChIP-seq, indicating that the chilling-induced H3K27me3 modification is reliable.Integrative analysis of transcriptome and ChIP-seq results showed that the expression of H3K27me3 target genes was significantly lower than those of non-target genes, indicating transcriptional repression of H3K27me3 in grapevine leaves. Furthermore, histone methylation alterations were detected in 82 genes and were related to either repression or activation of their expression during chilling stress. The findings provide the genome-wide H3K27me3 patterns in grapevines and shed light on uncovering its regulation in chilling stress responses. | Zhenfei Zhu Qingyun Li Duncan Kiragu Gichuki Yujun Hou Yuanshuang Liu Huimin Zhou Chen Xu Linchuan Fang Linzhong Gong Beibei Zheng Wei Duan Peige Fan Qingfeng Wang Haiping Xin | 2023 | Horticultural Plant Journal2023，9，3： | 1 |
| 7 | Injectable carriage 显示文摘 | Peige KT Cima L G Yaremchuk MJ | 1995 | Plast Reconst Surg1995，96，6： | 1 |
| 8 | Deficiency of aβ-arresttn-2 signal complex contributes to insulin resistance显示文摘 | Luan B ZhaoJ peiG etal | 2009 | Nature2009，457，7233： | 1 |
| 9 | Volatiles of grape berries evaluated at the germplasm level by headspace-SPME with GC–MS显示文摘 | Chunxiang Yang Yiju Wang Zhenchang Liang Peige Fan Benhong Wu Liu Yang Younian Wang Shaohua Li | 2008 | Food Chemistry2008，，3： | 1 |
| 10 | Anthoeyanin com- position and content in grape berry skin in Vitis germplasm显示文摘 | LIANG Zlaenchang WU Benhong FAN Peige | 2008 | Food Chemistry2008，111，4： | 1 |
| 11 | Gene body demethylation increases expression and is associated with self-pruning during grape genome duplication显示文摘A colchicine-induced autotetraploid grapevine exhibiting potentially valuable agronomic traits for grape production and breeding,including self-pruning,was identified.This study investigated DNA methylation variation and its role in gene expression during self-pruning in the autotetraploid grapevine.We used RNA-Seq to estimate differentially expressed genes between diploid and autotetraploid grapevine shoot tips.The genes showing increases in the autotetraploid were mainly related to stress response pathways,whereas those showing decreases in the autotetraploid were related to biological metabolism and biosynthesis.Whole-genome bisulfite sequencing was performed to produce single-base methylomes for the diploid and autotetraploid grapevines.Comparison between the methylomes revealed that they were conserved in CG and CHG contexts.In the autotetraploid grapevine,hypodifferentially methylated regions(DMRs)and hyper-DMRs in the gene body increased or decreased gene expression,respectively.Our results indicated that a hypo-DMR in the ACO1 gene body increased its expression and might promote self-pruning.This study reports that hypo-DMRs in the gene body increase gene expression in plants and reveals the mechanism underlying the changes in the modifications affecting gene expression during genome duplication.Overall,our results provide valuable information for understanding the relationships between DNA methylation,gene expression,and autotetraploid breeding in grape. | Luming Zou Wenwen Liu Zhan Zhang Everard J.Edwards Elias Kirabi Gathunga Peige Fan Wei Duan Shaohua Li Zhenchang Liang | 2020 | Horticulture Research2020，7，1： | 1 |
| 12 | Raopa tabu search algorithm with a new neighborhood structure for the job shop scheduling problem显示文摘 | ChaoYong Zhang PeiG enLi ZaiLin Guan YunQing | | 0，，： | 1 |
| 13 | Sugar and acid concentrations in 98 grape cultivars analyzed by principal component analysis显示文摘 | LIU Huaifeng WU Benhong FAN Peige | 2006 | Journal of the Science of Food and Agriculture2006，86，10： | 1 |
| 14 | Developmentmotoneuron cell death and neurotrophic factors显示文摘 | SendtnerM peiG BeckM | | 0，，： | 1 |
| 15 | Changes ofpolyphenols, sugars, and organic acid in 5 vitis genotypes during berryripening显示文摘 | LIANG Zhenchang SANG Min FAN Peige | 2011 | Journal of Food Science2011，76，9： | 1 |
| 16 | Sugar and acid concentrations in 98 grape cultivars analyzed by principal component analysis显示文摘 | LIU Huaifeng WU Benhong FAN Peige | 2006 | Journal of the Science of Food and Agriculture2006，86，10： | 1 |
| 17 | VvBBX44 and VvMYBA1 form a regulatory feedback loop to balance anthocyanin biosynthesis in grape显示文摘Anthocyanins are essential for the quality of perennial horticultural crops,such as grapes.In grapes,ELONGATED HYPOCOTYL 5(HY5)and MYBA1 are two critical transcription factors that regulate anthocyanin biosynthesis.Our previous work has shown that Vitis vinifera B-box protein 44(VvBBX44)inhibits anthocyanin synthesis and represses VvHY5 expression in grape calli.However,the regulatory mechanism underlying this regulation was unclear.In this study,we found that loss of VvBBX44 function resulted in increased anthocyanin accumulation in grapevine callus.VvBBX44 directly represses VvMYBA1,which activates VvBBX44.VvMYBA1,but not VvBBX44,directly modulates the expression of grape UDP flavonoid 3-O-glucosyltransferase(VvUFGT).We demonstrated that VvBBX44 represses the transcriptional activation of VvUFGT and VvBBX44 induced by VvMYBA1.However,VvBBX44 and VvMYBA1 did not physically interact in yeast.The application of exogenous anthocyanin stimulated VvBBX44 expression in grapevine suspension cells and tobacco leaves.These findings suggest that VvBBX44 and VvMYBA1 form a transcriptional feedback loop to prevent overaccumulation of anthocyanin and reduce metabolic costs.Our work sheds light on the complex regulatory network that controls anthocyanin biosynthesis in grapevine. | Wenwen Liu Huayuan Mu Ling Yuan Yang Li Yuting Li Shenchang Li Chong Ren Wei Duan Peige Fan Zhanwu Dai Yongfeng Zhou Zhenchang Liang Shaohua Li Lijun Wang | 2023 | Horticulture Research2023，10，10： | 0 |
| 18 | The class B heat shock factor HSFB1 regulates heat tolerance in grapevine显示文摘Grape is a widely cultivated crop with high economic value.Most cultivars derived from mild or cooler climates may not withstand increasing heat stress.Therefore,dissecting the mechanisms of heat tolerance in grapes is of particular significance.Here,we performed comparative transcriptome analysis of Vitis davidii‘Tangwei’(heat tolerant)and Vitis vinifera‘Jingxiu’(heat sensitive)grapevines after exposure to 25°C,40°C,or 45°C for 2 h.More differentially expressed genes(DEGs)were detected in‘Tangwei’than in‘Jingxiu’in response to heat stress,and the number of DEGs increased with increasing treatment temperatures.We identified a class B Heat Shock Factor,HSFB1,which was significantly upregulated in‘Tangwei’,but not in‘Jingxiu’,at high temperature.VdHSFB1 from‘Tangwei’and VvHSFB1 from‘Jingxiu’differ in only one amino acid,and both showed similar transcriptional repression activities.Overexpression and RNA interference of HSFB1 in grape indicated that HSFB1 positively regulates the heat tolerance.Moreover,the heat tolerance of HSFB1-overexpressing plants was positively correlated to HSFB1 expression level.The activity of the VdHSFB1 promoter is higher than that of VvHSFB1 under both normal and high temperatures.Promoter analysis showed that more TATA-box and AT∼TATA-box cis-elements are present in the VdHSFB1 promoter than the VvHSFB1 promoter.The promoter sequence variations between VdHSFB1 and VvHSFB1 likely determine the HSFB1 expression levels that inf luence heat tolerance of the two grape germplasms with contrasting thermotolerance.Collectively,we validated the role of HSFB1 in heat tolerance,and the knowledge gained will advance our ability to breed heat-tolerant grape cultivars. | Haiyang Chen Xinna Liu Shenchang Li Ling Yuan Huayuan Mu Yi Wang Yang Li Wei Duan Peige Fan Zhenchang Liang Lijun Wang | 2023 | Horticulture Research2023，10，3： | 0 |
| 19 | Constructing cactus-like mixed dimensional MOF@MOF as sorbent for extraction of bisphenols from environmental water显示文摘Metal-organic frameworks(MOFs)received considerable attention to adsorption and removal of various environmental pollutants because of some inherent advantages.However,it is challenging but meaningful to design and fabricate hierarchical mixed-dimensional MOFs with synergistic effects to enhance the performance for removal and preconcentration of environmental pollutants.Herein,a new hierarchical two-dimensional(2D)-three-dimensional(3D)mixed-dimensional cactus-like MOF@MOF hybrid material(PCN-134@Zr-BTB)was prepared by in-situ growth of 2D MOF nanosheets(Zr-BTB)on the surface of 3D MOF(PCN-134).The PCN-134@Zr-BTB composites combine the advantages of 2D and 3D MOFs with extensive mesoporous structures and large surface area for effective removal and enrichment of bisphenols(BPs).In comparison with pristine PCN-134 and Zr-BTB materials,the PCN-134@Zr-BTB hybrid material presented excellent adsorption performance for BPs.The adsorption isotherms are consistent with the Langmuir model,and the maximum adsorption capacity of four bisphenols(BPs)ranged from 135.1 mg/g to 628.9 mg/g.The adsorption kinetics are in accordance with the pseudo-second-order model.The recoveries ranged from 72.8%to 108%.The limits of detection were calculated at 0.02-0.03 ng/mL.The enrichment factors were calculated in the range of 310-374.According to FT-IR and XPS analysis,the main adsorption mechanisms are hydrogen bonding and π-π stacking.Nevertheless,this work provides a new and convenient strategy for the preparation of new hierarchical mixed-dimensional MOF@MOF(PCN-134@Zr-BTB)hybrid material for extraction and enrichment of BPs from aqueous matrix. | Peige Qin Shiping Zhu Mengyao Mu Yanmei Gao Zongwei Cai Minghua Lu | 2023 | Chinese Chemical Letters2023，34，12： | 0 |
| 20 | Basic leucine zipper gene VvbZIP61 is expressed at a quantitative trait locus for high monoterpene content in grape berries显示文摘The widely appreciated muscat flavor of grapes and wine is mainly attributable to the monoterpenes that accumulate in ripe grape berries.To identify quantitative trait loci(QTL)for grape berry monoterpene content,an F1 mapping population was constructed by a cross between two grapevine genotypes,one with neutral aroma berries(cv.‘Beifeng’)and the other with a pronounced muscat aroma(elite Vitis vinifera line‘3–34’).A high-density genetic linkage map spanning 1563.7 cM was constructed using 3332 SNP markers that were assigned to 19 linkage groups.Monoterpenes were extracted from the berry of the F1 progeny,then identified and quantified by gas chromatography–mass spectrometry.Twelve stable QTLs associated with the amounts of 11 monoterpenes in berries were thus identified.In parallel,the levels of RNA in berries from 34 diverse cultivars were estimated by RNA sequencing and compared to the monoterpene content of the berries.The expression of five genes mapping to stable QTLs correlated well with the monoterpene content of berries.These genes,including the basic leucine zipper VvbZIP61 gene on chromosome 12,are therefore considered as potentially being involved in monoterpene metabolism.Overexpression of VvbZIP61 in Vitis amurensis callus through Agrobacterium-mediated transformation significantly increased the accumulation of several monoterpenes in the callus,including nerol,linalool,geranial,geraniol,β-myrcene,and D-limonene.It is hypothesized that VvbZIP61 expression acts to increase muscat flavor in grapes.These results advance our understanding of the genetic control of monoterpene biosynthesis in grapes and provide important information for the marker-assisted selection of aroma compounds in grape breeding. | Yuyu Zhang Cuixia Liu Xianju Liu Zemin Wang Yi Wang Gan-yuan Zhong Shaohua Li Zhanwu Dai Zhenchang Liang Peige Fan | 2023 | Horticulture Research2023，10，9： | 0 |
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