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| 1 | Feeding infants and toddlersstudy:What foods are infants and toddlers eating? 显示文摘 | Fox MK Pac S Devaney B | 2004 | J AmDiet Assoc2004，104，11： | 1 |
| 2 | Early subclinical left-ventricular dysfunction in obese nonhypertensive children:a tissue Doppler imaging study显示文摘 | Kibar AE Pac FA Ballr S | 2013 | Pediatr Cardiol2013，34，6： | 1 |
| 3 | Early subclinical left-ventricular dysfunction in obese nonhypertensive children: a tissue Doppler ima- ging study显示文摘 | Kibar AE Pac FA Ball S | 2013 | Pediatr Cardio12013，34，6： | 1 |
| 4 | Stressor Specificity of Central Neuroendocrine Responses: Implications for Stress-Related Disorders显示文摘 | KAREL PACāK Miklós Palkovits | | Endocrine Rev0，22，4： | 1 |
| 5 | The behavior of the modified FX-LMS algorithm with secondary path modeling errors显示文摘 | Lopes PAC Piedade M S | 2004 | IEEE Signal Processing Letters2004，11，2： | 1 |
| 6 | Co-administration of plasmid expressing IL-12 with 14-kDa Schistosoma mansoni fatty acid-binding protein cDNA alters immune response profiles and fails to enhance protection induced by Sm14 DNA vaccine alone显示文摘 | Cristina T. Fonseca Lucila G.G. Pacífico Michele M. Barsante Tatiana Rassi Geovanni D. Cassali Sérgio. C. Oliveira | 2006 | Microbes and Infection2006，，9： | 1 |
| 7 | Fabrication of multilayer assemblies based on a boronate-terminated self-assembled monolayer 显示文摘 | Kato S Pac C | 1998 | Langmuir1998，14，： | 1 |
| 8 | Cross-sectional evaluation of circulating hepatitis B virus RNA and DNA: Different quasispecies?显示文摘BACKGROUND Different forms of pregenomic and other hepatitis B virus(HBV)RNA have been detected in patients’sera.These circulating HBV-RNAs may be useful for monitoring covalently closed circular DNA activity,and predicting hepatitis B eantigen seroconversion or viral rebound after nucleos(t)ide analog cessation.Data on serum HBV-RNA quasispecies,however,is scarce.It is therefore important to develop methodologies to thoroughly analyze this quasispecies,ensuring the elimination of any residual HBV-DNA.Studying circulating HBV-RNA quasispecies may facilitate achieving functional cure of HBV infection.AIM To establish a next-generation sequencing(NGS)methodology for analyzing serum HBV-RNA and comparing it with DNA quasispecies.METHODS Thirteen untreated chronic hepatitis B patients,showing different HBV-genotypes and degrees of severity of liver disease were enrolled in the study and a serum sample with HBV-DNA>5 Log10 IU/mL and HBV-RNA>4 Log10 copies/mL was taken from each patient.HBV-RNA was treated with DNAse I to remove any residual DNA,and the region between nucleotides(nt)1255-1611 was amplified using a 3-nested polymerase chain reaction protocol,and analyzed with NGS.Variability/conservation and complexity was compared between HBV-DNA and RNA quasispecies.RESULTS No HBV-DNA contamination was detected in cDNA samples from HBV-RNA quasispecies.HBV quasispecies complexity showed heterogeneous behavior among patients.The Rare Haplotype Load at 1%was greater in DNA than in RNA quasispecies,with no statistically significant differences(P=0.1641).Regarding conservation,information content was equal in RNA and DNA quasispecies in most nt positions[218/357(61.06%)].In 102 of the remaining 139(73.38%),HBV-RNA showed slightly higher variability.Sliding window analysis identified 4 hyper-conserved sequence fragments in each quasispecies,3 of them coincided between the 2 quasispecies:nts 1258-1286,1545-1573 and 1575-1604.The 2 hyper-variable sequence fragments also coincided:nts 1311-1344 and 1461-1485.Sequences between nts 1519-1543 and 1559-1587 were only hyper-conserved in HBV-DNA and RNA,respectively.CONCLUSION Our methodology allowed analyzing HBV-RNA quasispecies complexity and conservation without interference from HBV-DNA.Thanks to this,we have been able to compare both quasispecies in the present study. | Selene Garcia-Garcia Maria Francesca Cortese David Tabernero Josep Gregori Marta Vila Beatriz Pacín Josep Quer Rosario Casillas Laura Castillo-Ribelles Roser Ferrer-Costa Ariadna Rando-Segura Jesús Trejo-Zahínos Tomas Pumarola Ernesto Casis Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías | 2021 | World Journal of Gastroenterology2021，27，41： | 1 |
| 9 | Wind models for simulation of power fluctuations from wind farms显示文摘 | S(o)RENSEN P HANSEN AD ROSAS PAC | 2002 | Journal of Wind Engineering and Industrial Aerodynamics2002，90，12： | 1 |
| 10 | Quality speed conundrum: Trade offs in customer-intensive services 显示文摘 | Anand K Pac M F Veeraraghavan S K | 2011 | Management Science2011，57，1： | 1 |
| 11 | Current and future application of bone turnover markers显示文摘 | Christgau S Cloos PAC | 2003 | Clin Lab2003，49，910： | 1 |
| 12 | Quality-speed conundrum trade-offs in customer-intensive services显示文摘 | Anand K S Pac M F Veeraraghavan S | | 0，，01： | 1 |
| 13 | Meal and snack patterns of infants and toddlers显示文摘 | SKINNER JD ZIEGLER P PAC S | 2004 | J Am Diet Assoc2004，104，11： | 1 |
| 14 | Quality speed conundrum:Trade-offs in customer-intensive services显示文摘 | Anand K Pac M F Veeraraghavan S K | | 0，，1： | 1 |
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