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| 1 | Enhanced expression of interleukin-18 in serum and pancreas of patients with chronic pancreatitis显示文摘AIM: To investigate interleukin-18 (IL-18) in patients with chronic panreatitis (CP). METHODS: We studied 29 patients with CP and 30 healthy controls. Peripheral blood mononuclear cells (PBMC) were isolated and incubated with 50 mmol/L ethanol, lipopolysaccharide (LPS) (doses 25 g/L, 250 g/L, 2500 g/L) and both agents for 24 h. Levels of IL-18 in the supernatants, and levels of IL-18, IL-12, interferon (IFN)-γ and soluble CD14 in the serum were analysed by ELISA technique. Expression of IL-18 in PBMC was investigated by reverse-transcription (RT)-PCR. IL-18 protein levels in CP tissue and in normal pancreas were studied by ELISA technique. IL-18 levels in PBMC and pancreatic tissue were determined by Westernblot. Im-munohistochemistry for pancreatic IL-18 expression was performed. RESULTS: In patients, IL-18 serum levels were signifi-cantly enhanced by 76% (mean: 289.9 ± 167.7 ng/L) compared with controls (mean: 165.2 ± 43.6 ng/L; P < 0.0005). IL-12 levels were enhanced by 25% in patients (18.3 ± 7.3 ng/L) compared with controls (14.7 ± 6.8ng/L, P = 0.0576) although not reaching the statistical significance. IFN-γ and soluble CD14 levels were not in-creased. In vitro, LPS stimulated significantly and dose-dependently IL-18 secretion from PBMC. Incubation with ethanol reduced LPS-stimulated IL-18 secretion by about 50%. The mRNA expression of IL-18 in PBMC and the response of PBMC to ethanol and LPS was similar in CP patients and controls. In PBMC, no significant differences in IL-18 protein levels were detected between patients and controls. IL-18 protein levels were increased in CP tissues compared to normal pancreatic tissues. IL-18 was expressed by pancreatic acinar cells and by infiltrating inflammatory cells within the pancreas. CONCLUSION: IL-18 originates from the chronically inflammed pancreas and appears to be involved in the fibrotic destruction of the organ. | Alexander Schneider Stephan L Haas Ralf Hildenbrand Sren Siegmund Iris Reinhard Helmut Nakovics Manfred V Singer Peter Feick | 2006 | World Journal of Gastroenterology2006，12，40： | 4 |
| 2 | Incidence and clinical relevance of heparin induced antibodies in patients with deep vein thrombosis treated with unfractionated or low-molec ular-weight helSarin显示文摘 | Lindhoff LE Nakov R Misselwitx F | 2002 | Br J Hoemated2002，118，4： | 1 |
| 3 | The role of antisperm antibodies in the induction of immunologically- mediated human infertility显示文摘 | Dimit rova DK Kalaidzhiev SK Nakov LS | 2001 | Akush Ginekol (Sofiia)2001，40，3： | 1 |
| 4 | Incidence and clinical relevance of heparin induced antibodies in patients with deep vein thrombosis treated with unfractionated or low- molecular weight heparin 显示文摘 | Lindhoff- Last E Nakov R Misselwitz F | 2002 | Br J Haematol2002，118，4： | 1 |
| 5 | Darsentan:an effective endothelin A receptor antagonist for treatment of hypertension显示文摘 | Nakov R Pfarr E Eberle S | 2002 | Am J Hypertens2002，15，： | 1 |
| 6 | Effects of a low molecular weight heparin on thrombus regression and recurrent thromboembolism in patients with deep vein thrombosis 显示文摘 | Breddin HK Hach- Wunderle V Nakov R etal | 2001 | N Engl J Med2001，344，9： | 1 |
| 7 | The role of antisperm antibodies in the induction of immunologically-mediated human infertility显示文摘 | Dimitrova DK Kalaidzhiev SK Nakov LS | | 0，，： | 1 |
| 8 | Methods for the detection of antisperm antibodies accoclated with immunologically- mediated human infertility 显示文摘 | Dimitroa OK Kalamidzhiev SK Nakov LS | 2002 | Akush Gineclo (Sofiia)2002，41，3： | 1 |
| 9 | Antihypertensive therapy with verapamil SR plus trandolapfil versus atenolol plus chlorthalidone on glycemic control显示文摘 | Holzgreve H Nakov R Beck K | 2003 | Am J Hypertens2003，16，： | 1 |
| 10 | Classification of semantic relations between nominals显示文摘 | Girju R Nakov P Nastase V | 2009 | Language Resources and Evaluation2009，43，2： | 1 |
| 11 | The role of antisperm antibodies in the induction immunologically-mediated human infertility显示文摘 | Dimitrova DK Kalaidzhiev SK Nakov LS | 2001 | Akush Ginekol (Sofiia)2001，40，3： | 1 |
| 12 | Methods for the detection of antisperm antibodies associated with immunologically-mediated human infertility 显示文摘 | Dimitrora OK Kalaidzhiev SK Nakov LS | 2002 | Akush Ginekol (Sofiia)2002，41，3： | 1 |
| 13 | The role of antisperm antibodies in the induction immunologically_ mediated human infertility显示文摘 | Dimitrova DK Kalaidzhiev SK Nakov LS | 2001 | A Kush Ginekol Sofiia2001，40，3： | 1 |
| 14 | Methods for the seterction of antisperm antibodies asociated with immunologically mediated human infertility显示文摘 | Dimitrova DK Kalaidzhiev SK Nakov LS | 2002 | A Kush Ginekol Sofiia2002，41，3： | 1 |
| 15 | Methods for the detection of antisperm antibodies associated with immunologically-mediated human infertility显示文摘 | Dinitrora OK Kalaidzhiev SK Nakov LS | 2002 | Akush Ginekol (Soflia)2002，41，3： | 1 |
| 16 | the role of antisperm antibodies in the induction immunologically mediated human infertility 显示文摘 | Dimitrova DK Kalaidzhiev SK Nakov LS | 2001 | A Kush Ginekol sofiia2001，40，3： | 1 |
| 17 | Methods for the detection of antisperm antibodies associated with immunoiogically mediated human infertility显示文摘 | Dimitrova D K Kalaidzhiev S K Nakov L S | 2002 | Akush ginekol (sofila)2002，41，3： | 1 |
| 18 | The role of antisperm antibodies in the induction of immunologically - mediated human infertility 显示文摘 | Dimitrova DK Kalaidzhiev SK Nakov KS | 2001 | Akush Ginekol (Sofiia)2001，40，3： | 1 |
| 19 | Methods for the seterction of antisperm antibodies associated with immunologically mediated human infertility 显示文摘 | Dimitrova DK Kalaidzhiev SK Nakov LS | 2002 | A Kush Ginekol sofiia2002，41，3： | 1 |
| 20 | The role of antisperm antibodies in the induction immunologically_ mediated human infertility 显示文摘 | Dimitrova D K Kalaidzhiev S K Nakov L S | 2001 | A Kush Ginekol Sofiia2001，40，3： | 1 |
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