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| 1 | 从野生与突变株云芝IBL-04提纯锰过氧化物酶及其表征(英文)显示文摘由野生及突变株云芝IBL-04制得细胞外锰过氧化物酶(MnPs),并经过硫酸铵沉淀、透析、离子交换和凝胶渗透层析法等步骤提纯.纯化的酶在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)上于43 kDa区域呈现单一谱带,它适宜的pH值和温度分别为5.0和40°C.突变株MnPs表现出比野生株MnPs更宽的活性pH值范围和更高的热稳定性.从所选突变株所得纯化的MnPs表现出与野生株MnP几乎相同的电泳性质、稳态动力学、金属离子和EDCs降解效率.该生物酶与Mn^(2+)一起催化的反应速率最快,但最高的亲和性对应于ABTS、甲氧基羟苯基乙二醇、4-氨基苯酚和活性染料.Mn^(2+)和Cu^(2+)可显著提高MnPs的活性,但Zn^(2+),Fe^(2+),EDTA和半胱氨酸则会不同程度地抑制其活性,Hg^(2+)是最强的活性抑制剂.所有来源的MnPs均可有效催化EDCs、壬基苯酚和二氯苯氧氯酚降解,处理3 h可除去80%以上,在MnPs-介质体系中可进一步提高到90%.综上,云芝MnPs生物酶具有较高的pH适用性和热稳定性、独特的Michaelis-Menten动力学参数和高的EDCs去除效率等特点。 | Muhammad Asgher Muhammad Ramzan Muhammad Bilal | 2016 | Chinese Journal of Catalysis2016，37，4： | 4 |
| 2 | 小麦秸秆发酵产生的真菌Pleurotus ostreatus IBL-02锰过氧化物酶的溶胶-凝胶固定化及其催化污水脱色(英文)显示文摘Solid state bio-processing of wheat straw was carried out through an indigenous fungal strain Pleurotus ostreatus IBL-02 under pre-optimized fermentation conditions. The maximum activity, 692±12 U/mL, of the industrially important manganese peroxidase (MnP) enzyme was recorded after five days of still culture incubation. The crude MnP was 2.1-fold purified with a specific activity of 860 U/mg after purification on a Sephadex-G-100 gel column. On native and SDS-PAGE electrophoresis gels, the purified MnP fraction was a single homogenous band of 45 kDa. An active fraction of MnP was immobilized using hydrophobic sol-gel entrapment comprising tetramethoxysilane (T) and propyltrimethoxysilane (P) at different T:P molar ratios. Characterization revealed that after 24 h incubation at varying pH and temperatures, the MnP fraction immobilized at a T:P ratio of 1:2 in the sol-gel retained 82% and 75% of its original activity at pH4 and 70 ℃, respectively. The optimally active fraction at a 1:2 T:P ratio was tested against MnSO4 as a substrate to determine the kinetic catalytic constants KM and Vmax . To explore the industrial applicability of P. ostreatus IBL-02 MnP, both the free and immobilized MnP were used for the decolorization of four different textile industrial effluents. A maximum of 100% decolorization was achieved for the different textile effluents within the shortest time period. A lower KM , higher Vmax , hyper-activation, and enhanced acidic and thermal resistance up to 70 ℃ were the novel catalytic features of the sol-gel immobilized MnP, suggesting that it may be a potential candidate for biotechnological applications particularly for textile bioremediation purposes. | Muhammad Asgher Bazgha Aslam Hafiz Muhammad Nasir Iqbal | 2013 | 催化学报2013，34，9： | 4 |
| 3 | Hyperactivation and thermostabilization of Phanerochaete chrysosporium lignin peroxidase by immobilization in xerogels显示文摘 | Muhammad Asgher M. Javaid Asad Haq Nawaz Bhatti Raymond L. Legge | 2007 | World Journal of Microbiology and Biotechnology2007，，4： | 1 |
| 4 | Optimization of medium for decolorization d Solar golden yellow R direct textile dye by SchisophyUum commuae IBL-06显示文摘 | Muhammad Asgher Shahnaz Kausar Haq Nawaz Bhatti | 2008 | Intemation Biodeterioration &Biodegradation2008，61，： | 1 |
| 5 | Legge Recent developments in biodegradation of industrial pollutants by white rot fungi and their enzyme system 显示文摘 | Muhammad Asgher etl | 2008 | Biodegradation2008，，2008： | 1 |
| 6 | A comprehensive ligninolytic pre-treatment approach from lignocellulose green biotechnology to produce bio-ethanol显示文摘 | Muhammad Asgher Fareeha Bashir Hafiz Muhammad Nasir lqbal | 2013 | Chemical Engineering Research and Design2013，，： | 1 |
| 7 | Enhanced catalytic features of sol-gel immobilized MnP isolated from solid state culture of Pleurotus ostreatus IBL-02显示文摘An indigenous and industrially important manganese peroxidase(MnP) isolated from solid state culture of Pleurotus ostreatus IBL-02 was 2.1-fold purified and immobilized using sol-gel matrix of trimethoxysilane and proplytetramethoxysilane.Lower K_M.higher V_(max),hyper-activation,enhanced acidic and thermal stability up to 70 C,were the improved/enhanced catalytic features of the presently sol-gel immobilized MnP.A maximum of 100%decolorization was achieved within a short time period for the different real dyes containing textile industry effluents i.e.Nishat textile(NIT),K&N textile(KIT), Sitara textile(SIT),and Crescent textile(CRT). | Muhammad Asgher Hafiz Muhammad Nasir Iqbal | 2013 | Chinese Chemical Letters2013，24，4： | 0 |
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