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| 1 | Association between low molecular polypeptide 7 single nucleotide polymorphism and response to therapy in hepatitis C virus infection显示文摘AIM: To investigate the relationship between low molecular polypeptide-7 (LMP-7) gene polymorphism and response to interferon (IFN) therapy in chronic hepatitis C virus (HCV) patients. METHODS: LMP-7 polymorphism at codon 49 with nucleotide substitution from A to C was amplified in 104 chronic HCV patients of genotype 4. The amplicons were digested with restriction endonuclease Bsm I and the produced restriction fragment length polymorphism was analyzed. Patients received IFN + regional blood volume therapy for 48 wk and the frequency of thissingle nucleotide polymorphism (SNP) was statistically correlated with treatment response. The exclusion criteria for these patients were stated by the national health program for treating viral hepatitis. Main exclusion criteria included co-infection with hepatitis B virus or schistosomiasis, thyroid dysfunction, uncontrolled diabetes mellitus, history of long term drug or alcohol intake and autoimmune hepatitis. Multivariate analyses were done to correlate LMP-7 SNP plus several factors such as age, gender, weight, serum alpha-fetoprotein (AFP) and alanine aminotransferase levels, liver activity, fibrosis score and viral load with response to therapy. RESULTS: The data presented in this study clearly demonstrated statistically significant differences between sustained virological response (SVR) (defined as the absence of HCV RNA levels in the patient's sera at least 6 mo after discontinuation of treatment) and non-response (NR) (where HCV RNA levels in the patient's sera never become undetectable for 6 mo during or after treatment). Variables were described as odds ratio with 95%CI. The data were considered significant if P values were ≤ 0.05; highly significant if P < 0.01 and very highly significant if P < 0.001. Current data showed that 91.7% of patients carrying LMP-7 C/C allele were associated with SVR, while the other two genotypes C/A and A/A were associated with NR patients, 83.3% and 64.3% respectively, showing that genotype CC was strongly associated with response to interferon (95%CI: 12.0719-134.6572, P = 0.0001). The majority of parameters recorded in SVR and NR patients included higher values of mean age (P = 0.004), alanine aminotransferase (P = 0.001), AFP (P = 0.001), body weight (P = 0.025), viral load (P = 0.025), higher fibrosis and histological activity index indices among NR vs SVR patients. Also, the multivariate statistical analysis of the different factors of fibro-sis score, liver activity grade, genotypes and alleles of LMP-7 gene polymorphism in responders and NRs of HCV patients in this study showed that HCV patients with A allele had a very highly significant association with the NRs, high fibrosis and higher liver activity, while the C allele had a very highly significant association with the responders, low fibrosis and lower liver activity (95%CI: 3.5800-13.2519, P = 0.0001).CONCLUSION: LMP-7 SNP is a candidate gene that should be considered when designing a mathematical model for predicting response to therapy and disease progression in HCV patients. | Moataza H Omran Basma E Fotouh Samar S Youssef Noha E Ibrahim Wael Nabil EL-Sayed M Mahdy Wafaa G Shosha Mostafa K El-Awady | 2013 | World Journal of Hepatology2013，5，3： | 4 |
| 2 | Tumor necrosis factor-α-G308A polymorphism is associated with liver pathological changes in hepatitis C virus patients显示文摘AIM To investigate the association of tumor necrosis factor alpha(TNFα)-G308 A polymorphism with different liver pathological changes in treatment-na?ve Egyptian patients infected with hepatitis C virus(HCV) genotype 4.METHODS This study included 180 subjects,composed of 120 treatment-na?ve chronic HCV patients with different fibrosis grades(F0-F4) and 60 healthy controls. The TNFα-G308 A region was amplified by PCR and the different genotypes were detected by restriction fragment length polymorphism analysis. The TNFα protein was detected by enzyme-linked immunosorbent assay. The influence of different TNFα-G308 A genotypes on TNFα expression and liver disease progression were statistically analyzed. The OR and 95%CI were calculated to assess the relative risk confidence.RESULTS Current data showed that the TNFα-G308 A SNP frequency was significantly different between controls and HCV infected patients(P = 0.001). Both the AA genotype and A allele were significantly higher in late fibrosis patients(F2-F4,n = 60) than in early fibrosis patients(F0-F1,n = 60)(P = 0.05,0.04 respectively). Moreover,the GA or AA genotypes increased the TNFα serum level greater than the GG genotype(P = 0.002). The results showed a clear association between severe liver pathological conditions(inflammation,steatosis and fibrosis) and(GA + AA) genotypes(P = 0.035,0.03,0.04 respectively). The stepwise logistic regression analysis showed that the TNFα genotypes(GA + AA) were significantly associated with liver inflammation(OR = 3.776,95%CI: 1.399-10.194,P = 0.009),severe steatosis(OR = 4.49,95%CI: 1.441-14.0,P = 0.010) and fibrosis progression(OR = 2.84,95%CI: 1.080-7.472,P = 0.034). Also,the A allele was an independent risk factor for liver inflammation(P = 0.003),steatosis(P = 0.003) and fibrosis(P = 0.014). CONCLUSION TNFα SNP at nucleotide-308 represents an important genetic marker that can be used for the prognosis of different liver pathological changes in HCV infected | Noha G Bader El Din Sally Farouk Reem El-Shenawy Marwa K Ibrahim Reham M Dawood Mostafa M Elhady Ahmed M Salem Naglaa Zayed Ahmed Khairy Mostafa K El Awady | 2016 | World Journal of Gastroenterology2016，22，34： | 3 |
| 3 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 4 | COVID-19 and hepatic injury: cellular and molecular mechanisms in diverse liver cells显示文摘The coronavirus disease 2019(COVID-19)represents a global health and economic challenge.Hepatic injuries have been approved to be associated with severe acute respiratory syndrome coronavirus(SARS-CoV-2)infection.The viral tropism pattern of SARS-CoV-2 can induce hepatic injuries either by itself or by worsening the conditions of patients with hepatic diseases.Besides,other factors have been reported to play a crucial role in the pathological forms of hepatic injuries induced by SARS-CoV-2,including cytokine storm,hypoxia,endothelial cells,and even some treatments for COVID-19.On the other hand,several groups of people could be at risk of hepatic COVID-19 complications,such as pregnant women and neonates.The present review outlines and discusses the interplay between SARS-CoV-2 infection and hepatic injury,hepatic illness comorbidity,and risk factors.Besides,it is focused on the vaccination process and the role of developed vac-cines in preventing hepatic injuries due to SARS-CoV-2 infection. | Fares E M Ali Mostafa K Abd El-Aziz Mahmoud M Ali Osama M Ghogar Adel G Bakr | 2023 | World Journal of Gastroenterology2023，29，3： | 2 |
| 5 | Relationship between molecular compositions and rheological properties of neat asphalt binder at low and intermediate temperatures显示文摘 | Mostafa A E Louay N M Ionela G | | 0，，12： | 1 |
| 6 | Similarity analysis for effects of variable diffusivity and heat generation/absorption on heat and mass transfer for a MHD stagnation-point flow of a convective viscoelastic fluid over a stretching sheet with a slip velocity 显示文摘 | EL-HAWARY H M MOSTAFA A A MAHMOUD ABDEL- RAHMAN REDA G ABEER S E | 2013 | Mathematical Problems in Engineering2013，，： | 1 |
| 7 | Prediction of standard heats and Gibbs free energies formation of solid inorganic salts from group contributions显示文摘 | Mostafa A T M G Eakman J M Yarbro S L | | 0，，12： | 1 |
| 8 | Surgical treatment of displaced intra-articular catcaneal fracture using a single small lateral approach 显示文摘 | Mostafa M F E1-Adi G Hassanin E Y | 2010 | Strategies Trauma Limb Reconstr2010，5，2： | 1 |
| 9 | Nonlinear analysis of prestressed concrete box girder bridges under flexure显示文摘 | Razaqpur A G Nofal Mostafa Mirza M S | 1989 | Can J Civ Eng1989，16，1： | 1 |
| 10 | Effect of temperature and relative humidity on the cellular defense response of Ephestia kuehniella larvae fed Bacillus thuringiensis显示文摘 | MOSTAFA A M FIELDS P G HOLLIDAY N J | 2005 | Journal of Invertebrate Pathology2005，90，： | 1 |
| 11 | First derivative spectrophotometric determination of uranium(Ⅵ)and vanadium(Ⅴ)in natural and saline waters and some synthetic matrices using PAR and cetylpyridinum chloride显示文摘 | Abbas M N Homoda A M Mostafa G A E | 2001 | Anal Chim Acta2001，436，2： | 1 |
| 12 | First Derivative Spectrophotometric Determination of Uranium (Ⅵ) and Vanadium( Ⅴ ) in Natural and Saline Waters and Some Synthetic Matrices Using PAR and Cetylpyridinum Chloride显示文摘 | Abbas M N Homoda A M Mostafa G A E | 2001 | Analytica Chimica Acta2001，436，2： | 1 |
| 13 | Effects of prioritizing demand on optimal load shedding policy显示文摘 | Mostafa M A E1-Hawary M E Mbamalu G A N | 1996 | Electrical Power & Energy Systems1996，18，7： | 1 |
| 14 | Validated liquid chromatographic-fluorescence method for the quantita- tion of gemifloxacin in human plasma显示文摘 | AL-HADIYA B M KHADY AA MOSTAFA G A | 2010 | Talanta2010，83，1： | 1 |
| 15 | Effects of isolated Lactobacillus acidophilus as a probiotic on chicken vaccinated and infected with Salmonella typhimurium 显示文摘 | Youssef G A Ezzeldeen N A Mostafa A M etal | 2011 | Global Veterinaria2011，7，5： | 1 |
| 16 | Nonlinear analysis of prestressed concrete box girder bridges under flexure 显示文摘 | RAZAQPUR A G NOFAI MOSTAFA MIRZA M S | 1989 | Canadian Journal of Civil Engineering1989，16，1： | 1 |
| 17 | Sallylc-ysteine ameliorates doxorubicin toxicity in the heart and liver of mice显示文摘 | MOSTAFA M G MIMA T OHNISHI S T | 2000 | Planta Med2000，66，2： | 1 |
| 18 | Determination of Traces of Nitrite and Nitrate in Water by Solid Phase Spectrophotometry显示文摘 | Abbas M N Mostafa G A | 2000 | Anal Chim Acta2000，410，： | 1 |
| 19 | Autoantibody to heterogeneous nuclear ribonucleoprotein-A2 ( RA33 ) in juvenile idiopathic arthritis : clinical significance 显示文摘 | Tomoum H Y Mostafa G A E1-Shahat E M | 2009 | Pediatr lnt2009，51，2： | 1 |
| 20 | Do Liquid-Based Preparations of Urinary Cytology Perform Differently Than Classically Prepared Cases? Observations From the College of American Pathologists Interlaboratory Comparison Program in Nongynecologic Cytology显示文摘 | Laucirica Rodolfo Bentz Joel S Souers Rhona J Wasserman Patricia G Crothers Barbara A Clayton Amy C Henry Michael R Chmara Beth Anne Clary Karen M Fraig Mostafa M Moriarty Ann T | 2010 | Archives of Pathology & Laboratory Medicine2010，，1： | 1 |
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