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		题名	作者	年代	出处	被引量
	1	Absolute copy number differences of Y chromosomal genes between crossbred (Bos taurus×Bos indicus) and Indicine bulls显示文摘Background: The Y chromosome in mammal is paternally inherited and harbors genes related to male fertility and spermatogenesis. The unique intra-chromosomal recombination pattern of Y chromosome and morphological difference of this chromosome between Bos taurus and Bos indicus make it an ideal model for studying structural variation, especially in crossbred(Bos taurus × Bos indicus) bulls. Copy Number Variation(CNV) is a type of genomic structural variation that gives information complementary to SNP data. The purpose of this study was to find out copy number differences of four Y chromosomal spermatogenesis-related candidate genes in genomic DNA of crossbred and purebred Indicine bulls. Result: Four Y chromosomal candidate genes of spermatogenesis namely, sex determining gene on Y chromosome(SRY), DEAD box polypeptide 3-Y chromosome(DDX3Y), Ubiquitin specific peptidase 9, Y-linked(USP9Y), testis-specific protein on Y chromosome(TSPY) were evaluated. Absolute copy numbers of Y chromosomal genes were determined by standard curve-based quantitative real time PCR. Copy numbers of SRY and TSPY genes per unit amount of genomic DNA are higher in crossbred than Indicine bulls. However, no difference was observed in DDX3Y and USP9Y gene copy numbers between two groups. Conclusion: The present study demonstrates that the structural organization of Y chromosomes differs between crossbred and Indicine bulls which are reproductively healthy as observed from analysis of semen attributes. The absolute copy numbers of SRY and TSPY genes in unit mass of genomic DNA of crossbred bulls are significantly higher than Indicine bulls. No alteration in absolute copies of DDX3Y and USP9Y gene was found between the genome of crossbred and Indicine bulls. This study suggests that the DDX3Y and USP9Y are likely to be single copy genes in the genome of crossbred and Indicine bulls and variation in Y chromosome length between crossbred and Indicine bulls may be due to the copy number variation of SRY gene and TSPY array.	Ayan Mukherjee Gulshan Dass Jagan Mohanarao G Moloya Gohain Biswajit Brahma Tirtha Kumar Datta Sachinandan De	2013	Journal of Animal Science and Biotechnology2013，4，4：	3
	2	Identification of capacitation associated tyrosine phosphoproteins in buffalo ( Bubalus bubalis ) and cattle spermatozoa显示文摘	G. Jagan Mohanarao S.K. Atreja	2010	Animal Reproduction Science2010，，1：	1
	3	Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo ( Bubalua bubalis) sperm quality following cryopreservation 显示文摘	Reddy N S S Mohanarao G J Atreja S K	2010	Animal Re- production Science2010，119，：	1
	4	Identification of capacitation associated tyrosine phosphoproteins in buffalo(Bubalus bubalis)and cattle spermatozoa显示文摘	Jagan Mohanarao G Atreja S K	2011	Anim Reprod Sci2011，123，12：	1
	5	Freeze-thaw induced genotoxicity in buffalo(Bubalus bubalis) spermatozoa in relation to total antioxidant status 显示文摘	Kumar R Mohanarao G J Arvind	2011	Molecular Biology Reports2011，38，3：	1
	6	Identification of capacitation associat-ed tyrosine phosphoproteins in buffalo ( Bubalus bubalis) and cattlespermatozoav显示文摘	jagan Mohanarao G Atreja SK	2011	Anim Reprod Sci2011，123，12：	1
	7	Freeze-thaw induced genotoxicity in buffalo (Bubalus bubalis) spermatozoa in relation to total antioxidant status显示文摘	Kumar R Mohanarao G J Arvind	2011	Molecular Biology Reports2011，38，3：	1

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