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| 1 | The role of gut microbiota(commensal bacteria)and the mucosal barrier in the pathogenesis of inflammatory and autoimmune diseases and cancer:contribution of germ-free and gnotobiotic animal models of human diseases显示文摘Metagenomic approaches are currently being used to decipher the genome of the microbiota(microbiome),and,in parallel,functional studies are being performed to analyze the effects of the microbiota on the host.Gnotobiological methods are an indispensable tool for studying the consequences of bacterial colonization.Animals used as models of human diseases can be maintained in sterile conditions(isolators used for germ-free rearing)and specifically colonized with defined microbes(including non-cultivable commensal bacteria).The effects of the germ-free state or the effects of colonization on disease initiation and maintenance can be observed in these models.Using this approach we demonstrated direct involvement of components of the microbiota in chronic intestinal inflammation and development of colonic neoplasia(i.e.,using models of human inflammatory bowel disease and colorectal carcinoma).In contrast,a protective effect of microbiota colonization was demonstrated for the development of autoimmune diabetes in non-obese diabetic(NOD)mice.Interestingly,the development of atherosclerosis in germ-free apolipoprotein E(ApoE)-deficient mice fed by a standard low-cholesterol diet is accelerated compared with conventionally reared animals.Mucosal induction of tolerance to allergen Bet v1 was not influenced by the presence or absence of microbiota.Identification of components of the microbiota and elucidation of the molecular mechanisms of their action in inducing pathological changes or exerting beneficial,disease-protective activities could aid in our ability to influence the composition of the microbiota and to find bacterial strains and components(e.g.,probiotics and prebiotics)whose administration may aid in disease prevention and treatment. | Helena Tlaskalova-Hogenova Renata Stepankova Hana Kozakova Tomas Hudcovic Luca Vannucci Ludmila Tuckova Pavel Rossmann TomasHrncır Miloslav Kverka Zuzana Zakostelska Klara Klimesova Jaroslava Pribylova Jirina Bartova Daniel Sanchez Petra Fundova Dana Borovska Dagmar Sru˚tkova Zdenek Zıdek Martin Schwarzer Pavel Drastich David P Funda | 2011 | Cellular & Molecular Immunology2011，8，2： | 47 |
| 2 | Distinct gut microbiota profiles in patients with primary sclerosing cholangitis and ulcerative colitis显示文摘AIM To characterize the gut bacterial microbiota of patients with primary sclerosing cholangitis(PSC) and ulcerative colitis(UC).METHODS Stool samples were collected and relevant clinical data obtained from 106 study participants, 43 PSC patients with(n = 32) or without(n = 11) concomitant inflammatory bowel disease, 32 UC patients, and 31 healthy controls. The V3 and V4 regions of the 16 S ribosomal RNA gene were sequenced on Illumina Mi Seq platform to cover low taxonomic levels. Data were further processed in QIIME employing Ma As Lin and LEf Se tools for analysis of the output data.RESULTS Microbial profiles in both PSC and UC were characterized by low bacterial diversity and significant change in global microbial composition. Rothia, Enterococcus, Streptococcus, Veillonella, and three other genera were markedly overrepresented in PSC regardless of concomitant inflammatory bowel disease(IBD). Rothia, Veillonella and Streptococcus were tracked to the species level to identify Rothia mucilaginosa, Streptococcus infantus, S. alactolyticus, and S. equi along with Veillonella parvula and V. dispar. PSC was further characterized by decreased abundance of Adlercreutzia equolifaciens and Prevotella copri. Decrease in genus Phascolarctobacterium was linked to presence of colonic inflammation regardless of IBD phenotype. Akkermansia muciniphila, Butyricicoccus pullicaecorum and Clostridium colinum were decreased in UC along with genus Roseburia. Low levels of serum albumin were significantly correlated with enrichment of order Actinomycetales.CONCLUSION PSC is associated with specific gut microbes independently of concomitant IBD and several bacterial taxa clearly distinguish IBD phenotypes(PSC-IBD and UC). | Lukas Bajer Miloslav Kverka Martin Kostovcik Peter Macinga Jiri Dvorak Zuzana Stehlikova Jan Brezina Pavel Wohl Julius Spicak Pavel Drastich | 2017 | World Journal of Gastroenterology2017，23，25： | 22 |
| 3 | 广州市不同类型水体轮虫群落结构的时空变动及与理化因子间的关系显示文摘轮虫是水体中重要的浮游生物类群,对环境变化敏感,是水生态系统中食物链及微型食物网的关键环节.于2015年夏季(7月)和冬季(12月)对养殖池塘、水库、广州城市湖泊、珠江河口及珠江河段水域的轮虫和理化环境指标进行了调查分析,共发现轮虫26属、76种,其中裂痕龟纹轮虫(Anuraeopsis fissa)在7月河流水体占绝对优势,但12月数量明显减少.冬季珠江河段和河口水体群落多样性指数与均匀度指数较夏季高,群落结构较夏季稳定.调查水体轮虫丰度范围为33~2625 ind./L,城市湖泊夏季丰度较高,冬季有所下降,而养殖池塘轮虫丰度在冬季有所上升.群落结构相似性分析(ANOSIM)分析表明,不同类型水体之间差异显著,尤以湖泊与河流差异性最大,广布多肢轮虫(Polyarthra vulgaris)在湖泊贡献率最高,暗小异尾轮虫(Trichocerca pusilla)其次;裂痕龟纹轮虫在河流贡献率最高,优势种丰度差异是造成湖泊与河流群落结构差异的主要原因.统计分析表明,轮虫丰度与叶绿素a浓度呈正相关.冗余分析与ANOSIM分析发现广布多肢轮虫在流花湖等叶绿素a浓度较高的静态水体中易形成优势;裂痕龟纹轮虫和角突臂尾轮虫(Brachionus angularis)在珠江河段等总氮和总磷浓度高的富营养化流动水体中易形成优势.综合轮虫群落结构和水质特征,广州市水体富营养化严重,耐污性轮虫种类多,应加强城市水生态系统保护和管理. | 梁迪文 王庆 魏南 Miloslav Devetter 杨宇峰 | 2017 | 湖泊科学2017，29，6： | 14 |
| 4 | Laparoscopic approach to retrorectal cyst显示文摘Retrorectal cysts are rare benign lesions in the presacral space which are frequently diagnosed in middle-aged females. We report here our experience with two symptomatic female patients who were diagnosed as having a retrorectal cyst and managed using a laparoscopic approach. The two patients were misdiagnosed as having an ovarian cystic lesion after abdominal ultrasonography. Computer tomograghy (CT) scan was mandatory to establish the diagnosis. The trocar port site was the same in both patients. An additional left oophorectomy was done for a coexisting ovarian cystic lesion in one patient in the same setting. There was no postoperative morbidity or mortality and the two patients were discharged on the 5th and 6th post operative days, respectively. Our cases show that laparoscopic management of retrorectal cysts is a safe approach. It reduces surgical trauma and offers an excellent tool for perfect visualization of the deep structures in the presacral space. | Petra Gunkova Lubomir Martinek Jan Dostalik Igor Gunka Petr Vavra Miloslav Mazur | 2008 | World Journal of Gastroenterology2008，14，42： | 4 |
| 5 | Lunar-based Ultraviolet Telescope study of the well-known Algol-type binary TW Dra显示文摘By using the Lunar-based Ultraviolet Telescope(LUT) from 2014 December 2 to December 4,the first near-UV light curve of the well-known Algol-type binary TW Dra is reported,which is analyzed with the 2013 version of the W-D code.Our solutions confirmed that TW Dra is a semi-detached binary system where the secondary component fills its Roche lobe.The mass ratio and a high inclination are obtained(q = 0.47,i = 86.68°).Based on 589 available data spanning more than one century,the complex period changes are studied.Secular increase and three cyclical changes are found in the corresponding orbital period analysis.The secular increase changes reveal mass transfer from the secondary component to the primary one at a rate of 6.8 × 10^(-7)M_⊙yr^(-1).One large cyclical change of 116.04 yr may be caused by disturbance of visual component ADS 9706 B orbiting TW Dra(ADS 9706A),while the other two cyclical changes with shorter periods of 22.47 and 37.27 yr can be explained as the result of two circumbinary companions that are orbiting around TW Dra,where the two companions are in simple 3 :5 orbit-rotation resonances.TW Dra itself is a basic binary in a possible sextuple system with the configuration(1 + 1) +(1 + 1) +(1 + 1),which further suggests that multiplicity may be a fairly common phenomenon in close binary systems. | Wen-Ping Liao Sheng-Bang Qian Miloslav Zejdat Li-Ying Zhu Lin-Jia Li | 2016 | Research in Astronomy and Astrophysics2016，16，6： | 2 |
| 6 | Developmet of an amperometric L-ascorbic acid (Vitamin C)sensor based on electropolymerised aniline for pharmaceutical and food analysis显示文摘 | Philip J O' Connell Cicile Gormally Miloslav Pravda | 2001 | Analytica Chimica Acta2001，431，： | 2 |
| 7 | New species of Cryptosporidium Tyzzer, 1907 (Apicomplexa) from amphibian host: morphology, biology and phylogeny显示文摘 | Jirku Miloslav Valigurová Andrea Koudela Bretislav Krízek Jaroslav Modry David Slapeta Jan | 2008 | Folia Parasitologica2008，，2： | 1 |
| 8 | Municipal sewage sludge characteristics and waste water treatment plant effectiveness under warm climate conditions显示文摘 | Antonis. A. Zorpas Chryso Coumi Miloslav Drtil Irene Voukalli | 2011 | Desalination and Water Treatment . 2011 (1-3)2011，，1： | 1 |
| 9 | Effects of excretal returns and soil compaction on nitrous oxide emissions from a cattle overwintering area Agriculture显示文摘 | MILOSLAV SIMEK PETR BRUCEK JAROSLAV HYNST Petersen | 2006 | Ecosystems and Environment2006，112，： | 1 |
| 10 | Development of a disposable immunosensor for the detection of human heart fatty-acid binding protein in human whole blood using screen-printed carbon electrodes显示文摘 | Regan O Tina M Pravda Miloslav | 2002 | Talant2002，57，3： | 1 |
| 11 | Determination of some quinoxaline-N-dioxidederivatives by adsorptive stripping voltammetry Analytica Chimica Acta显示文摘 | V ra Stara Miloslav Kopanica | 1986 | 186:21 - 301986，186，： | 1 |
| 12 | Cultivar dif- ferences of total anthocyanins and anthocyanidins in red and pur- ple- fleshed potatoes and their relation to antioxidant activity 显示文摘 | Lachman Jaromir Hamouz Karel Sulc Miloslav | 2009 | Food Chemistry2009，114，3： | 1 |
| 13 | Recent applications of electrogenerated chemiluminescenee in chemical analysis显示文摘 | Karsten A Fahnrich Miloslav Pravda George G Guilbault | 2001 | Talanta2001，54，： | 1 |
| 14 | Screening for testosterone, methyltestosterone, 19-nortestosterone residues and their metabolites in bovine urine with enzyme-linked immunosorbent assay (ELISA)显示文摘 | Huihui Lu Grainne Conneely Mark A. Crowe Margaret Aherne Miloslav Pravda George G. Guilbault | 2006 | Analytica Chimica Acta2006，，1： | 1 |
| 15 | Comparison of the total antioxidant status of Bohemian wines during the wine- making process 显示文摘 | JAROMIR LACHMAN MILOSLAV SULC MAREK SCHILLA | 2007 | Food Chemistry2007，10，3： | 1 |
| 16 | Biosorption and biovolatilization of arsenic by heat-resistant fungi显示文摘 | Slavomír ?erňansky Martin Urík Jaroslav ?evc Miloslav Khun | 2007 | Environmental Science and Pollution Research - International2007，，1： | 1 |
| 17 | Fluidization of dried wastewater sludge显示文摘 | Miloslav Hartman Michael Pohoǐel Otakar Trnka | 2007 | Powder Technology2007，178，3： | 1 |
| 18 | Microstructure and Physical Properties of a Reduced Fat Mozzarella Cheese Made Using Lactobacillus casei ssp. casei Adjunct Culture显示文摘 | Richard K. Merrill Craig J. Oberg William R. McManus Miloslav Kalab Donald J. McMahon | 1996 | LWT - Food Science and Technology1996，，8： | 1 |
| 19 | Calculated Lattice Energies of Energetic Materials in a Prediction of their Heats of Fusion and Sublimation显示文摘The paper specifies an unambiguous basic relationship between the published results of ab initio calculations of lattice energies, E L,and heats of sublimation, ΔH s,of individual energetic materials. In this relationship,the ΔH s value has been replaced by heats of fusion, ΔH m,tr . Thereby its unambiguity has been lost,and the similarity of details of molecular structure begins to be of decisive importance. The resulting partial relationships,together with the basic relationship,have been used for prediction of ΔH s,and ΔH m,tr values of technically attractive polynitro compounds. | Svatopluk Zeman, Miloslav Krupka (Department of Theory and Technology of Explosives,University of Pardubice CZ 532 10 Pardubice, Czech Republic) | 2002 | 含能材料2002，10，1： | 1 |
| 20 | Comparison of the total antioxidant status of Bohemian wines during the wine-making process显示文摘 | Jaromir Lachman Miloslav Sulc Marek Schilla | 2007 | Food Chemistry2007，103，： | 1 |
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