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| 1 | MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean显示文摘Male sterility is an essential trait in hybrid seed production,especially for monoclinous and autogamous food crops.Soybean male-sterile ms1 mutant has been known for more than 50 years and could be instrumental in making hybrid seeds.However,the gene responsible for the male-sterile phenotype has remained unknown.Here,we report the map-based cloning and characterization of the MS1 gene in soybean.MS1 encodes a kinesin protein and localizes to the nucleus,where it is required for the male meiotic cytokinesis after telophase Ⅱ.We further substantiated that MS1 colocalizes with microtubules and is essential for cell plate formation in soybean male gametogenesis through immunostaining.Both ms1 and CRISPR/Cas9 knockout mutants show complete male sterility but are otherwise phenotypically normal,making them perfect tools for producing hybrid seeds.The identification of MS1 has the practical potential for assembling the sterility system and speeding up hybrid soybean breeding. | Xiaolong Fang Xiaoyuan Sun Xiangdong Yang Qing Li Chunjing Lin Jie Xu Wenjun Gong Yifan Wang Lu Liu Limei Zhao Baohui Liu Jun Qin Mengchen Zhang Chunbao Zhang Fanjiang Kong Meina Li | 2021 | Science China(Life Sciences)2021，64，9： | 4 |
| 2 | Genetic Variation Dissection of Rice Blast Resistance Using an Indica Population显示文摘Rice blast disease,caused by fungus pathogen Magnaporthe oryzae,is one of the most destructive diseases that impact rice farming.In the worldwide,rice harvests lose 10%to 30%of the total production because of blast infection,which is estimated to be enough for feeding 60 million people(Skamnioti and Gurr,2009). | ZHANG Mengchen WEI Zhonghua YUAN Xiaoping WANG Caihong WANG Shan NIU Xiaojun XU Xin XU Qun FENG Yue YU Hanyong WANG Yiping ZHU Zhiwei ZHAI Rongrong YANG Yaolong WEI Xinghua | 2020 | Rice science2020，27，4： | 1 |
| 3 | Fatal spontaneous rupture of common iliac artery associated with fibromuscular dysplasia显示文摘A previously healthy 25-year-old man with no known risk factors was presented at the emergency room with a 3 h history of abdominal and loin pain.Physical examination and lab data showed no specific findings except tenderness,slight white cell count elevation and decreased haemoglobin level.The patient’s condition deteriorated over the following hours and he died despite resuscitation attempts.Autopsy revealed a 2.5-cm longitudinal tear in the intima of the right common iliac artery,which formed a thrombosed false lumen extending to the abdominal aorta proximally and to the left common iliac artery.Histopathologic examination revealed the characteristic changes of fibromuscular dysplasia(FMD).FMD involving the common iliac arteries is extremely rare;only six cases have been reported previously,and only two of those included forensic findings.The presented case is the first case of FMD with intimal tearing in the right common iliac artery,with propagation to the left common iliac artery and abdominal aorta.When a previously healthy young adult without hypertension or other risk factors presents with acute abdominal and loin pain,systemic vascular disease should be on the list of differential diagnoses.Careful and complete evaluation of multiple arteries can be critical. | Xiang Xu Mengchen Tsai Ning Xiao Xiaohui Tan Fu Zhang Yangeng Yu Qi Wang Weibing Xie Huijun Wang Dongri Li | 2019 | Forensic Sciences Research2019，4，4： | 1 |
| 4 | Brittle culm 25, which encodes an UDP-xylose synthase, affects cell wall properties in rice显示文摘Because plant mechanical strength influences plant growth and development,the regulatory mechanisms underlying cell-wall synthesis deserve investigation.Rice mutants are useful for such research.We have identified a novel brittle culm 25(bc25)mutant with reduced growth and partial sterility.BC25 encodes an UDP-glucuronic acid decarboxylase involved in cellulose synthesis and belongs to the UXS family.A single-nucleotide mutation in BC25 accounts for its altered cell morphology and cellwall composition.Transmission electron microscopy analysis showed that the thickness of the secondary cell wall was reduced in bc25.Monosaccharide analysis revealed significant increases in content of rhamnose and arabinose but not of other monosaccharides,indicating that BC25 was involved in xylose synthesis with some level of functional redundancy.Enzymatic assays suggested that BC25 functions with high activity to interconvert UDP-glucuronic acid(UDP-Glc A)and UDP-xylose.GUS staining showed that BC25 was ubiquitously expressed with higher expression in culm,root and sheath,in agreement with that shown by quantitative real-time(q RT)-PCR.RNA-seq further suggested that BC25 is involved in sugar metabolism.We conclude that BC25 strongly influences rice cell wall formation. | Siliang Xu Mengchen Zhang Junhua Ye Dongxiu Hu Yuanyuan Zhang Zhen Li Junrong Liu Yanfei Sun Shan Wang Xiaoping Yuan Yue Feng Qun Xu Xinghua Wei Dali Zeng Yaolong Yang | 2023 | The Crop Journal2023，11，3： | 0 |
| 5 | SARS-CoV-2 encoded microRNAs are involved in the process of virus infection and host immune response显示文摘The outbreak of COVID-19 caused by SARS-CoV-2 is spreading worldwide,with the pathogenesis mostly unclear.Both virus and host-derived microRNA(miRNA)play essential roles in the pathology of virus infection.This study aims to uncover the mechanism for SARS-CoV-2 pathogenicity from the perspective of miRNA.We scanned the SARS-CoV-2 genome for putative miRNA genes and miRNA targets and conducted in vivo experiments to validate the virus-encoded miRNAs and their regulatory role on the putative targets.One of such virus-encoded miRNAs,MR147-3p,was overexpressed that resulted in significantly decreased transcript levels of all of the predicted targets in human,i.e.,EXOC7,RAD9A,and TFE3 in the virus-infected cells.The analysis showed that the immune response and cytoskeleton organization are two of the most notable biological processes regulated by the infection-modulated miRNAs.Additionally,the genomic mutation of SARS-CoV-2 contributed to the changed miRNA repository and targets,suggesting a possible role of miRNAs in the attenuated phenotype of SARS-CoV-2 during its evolution.This study provided a comprehensive view of the miRNA-involved regulatory system during SARS-CoV-2 infection,indicating possible antiviral therapeutics against SARS-CoV-2 through intervening miRNA regulation. | Zhi Liu Jianwei Wang Yiyue Ge Yuyu Xu Mengchen Guo Kai Mi Rui Xu Yang Pei Qiankun Zhang Xiaoting Luan Zhibin Hu Ying Chi Xingyin Liu | 2021 | The Journal of Biomedical Research2021，35，3： | 0 |
| 6 | Fabrication of patterned transparent conductive glass via laser metal transfer for efficient electrical heating and antibacteria显示文摘Vapor deposition and three-dimensional(3D)printing technology are considered to be conventional methods to achieve patterned metal film preparation through the assistance of masks and high temperature.Therefore,there are still some challenges in fabricating metal films in template-free and normal temperature environment.In this work,we report a flexible and rapid laser metal transfer(LMT)technique for fabricating the various metal films(Cu,Ni,Sn,Al,Fe,and Ag)with different patterns without templates on arbitrary substrates(glass,polyimide(PI)films,and aluminum nitride(AlN)ceramic).Especially,the obtained transparent conductive glass displays high transmittance(more than 90%)and adjustable resistances(≈5Ω).According to the Joule effect,the interface resistance between Cu particles and copper oxide coating produces the high temperature approximately 280℃ at 2 V in a short time(≈60 s)and remains stable at 120℃ over 12 h.At last,the multifunctional glass with Cu patterns also shows excellent bactericidal activity(≈95%).This work demonstrates that laser metal transfer is an exceeding effective means of fabricating the micro/nano structures with potential applications in functional devices. | Xiaoyan Liu Ting Zhang Mengchen Xu Yang Li Haiqing Wang Yuke Chen Xuzihan Zhang Zenan Wang Xiaoyan Li Weijia Zhou Hong Liu | 2024 | Nano Research2024，17，3： | 0 |
| 7 | A Study on the Driving Factors of Food Production in Huang-Huai-Hai Plain Based on Path Analysis显示文摘The effects of 14 factors on food production in Huang-Huai-Hai Plain are analyzed by path analysis in this paper,and then the linear regression models of them are established by SPSS software. The results show that electricity consumption for agriculture,growing area of crops,the affected area,annual average temperature and arable land area at the end of the year have great effects on food production. Finally some recommendations are put forward to improve the food production in Huang-Huai-Hai Plain such as improving the level of agricultural mechanization,stabilizing food production,preventing natural disasters and increasing the effective irrigation area. | Yaqi LIU Jiazhen LIU Jinping ZHANG Yongjin CHEN Mengchen XU Chengxiang WANG | 2015 | Asian Agricultural Research2015，7，7： | 0 |
| 8 | Design of Multi-unit Control System of High Voltage Power Supply for LASCAR Scintillator Detector Array显示文摘The power voltages of Photomultipliers (PMTs) at RIBLL LASCAR scintillator detector array are distributed between 900 V and 1 800 V irregularly. 392 CC123 modules are employed to supply high voltage for the PMT array. The CC123 module serves as PMT interface groupware package, and it can transform +12 V DC input voltage to ranges of 0~-2200 V for the PMT power supply corresponding to 0~+5 V output voltage from the control board crate. The relation of PMT power supply with the output voltage of the control crate is shown in Fig.1. | Wu Lijie, Wang Jinchuan, Xiao Guoqing, Guo Zhongyan, Zhan Wenlong, Qi Huirong, Xu ZhiguoZhang Li, Ding Xianli, Xu Hushan, Sun Zhiyu, Li Jiaxing, Li Chen, Wang MengChen Lixin, Hu Zhengguo, Mao Ruishi and Zhao Tiecheng | 2003 | 近代物理研究所和兰州重离子加速器实验室年报:英文版2003，，1： | 0 |
| 9 | Swertiamarin ameliorates diet-induced obesity by promoting adipose tissue browning and oxidative metabolism in preexisting obese mice显示文摘Obesity is a risk factor for many metabolic diseases.Efficient therapeutic strategies are urgently needed.Swertiamarin(STM)prevents obesity and the associated insulin resistance and inflammation.However,the therapeutic effects of STM on preexisting obesity remain unclear.Therefore,in this study we aim to investigate the effects of STM on energy expenditure and fat browning in mice with preexisting obesity.C57BL/6J mice are fed with a high-fat diet(HFD)for 8 weeks to induce obesity and then gavaged(or not)with STM for 10 weeks.The whole-body energy metabolism of mice is examined by indirect calorimetry.The results show that after 10 weeks of treatment,STM markedly prevents HFD-induced weight gain,chronic inflammation,insulin resistance,and hepatic steatosis.STM promotes oxygen consumption and energy expenditure.The level of uncoupling protein 1 is enhanced in the brown and white adipose tissues of STM-treated mice.STM increases the phosphorylation of AMP-activated protein kinase and the expressions of genes involved in fat oxidation,reducing fat deposition in skeletal muscles.Meanwhile,STM does not affect the intestinal microbiotic composition.Overall,STM supplementation may serve as a potential therapy for obesity. | Yuqin Zhu Haoran Li Mengchen Ma Dandan Li Ouyang Zhang Suili Cai Yajiao Wang Dandan Chen Shengnan Jin Chunming Ding Liang Xu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，1： | 0 |
| 10 | Classification and Identification of indica P/TGMS Lines in China显示文摘Despite extensive studies on cultivated rice, the population structure and genetic diversity of the indica photoperiod-and thermo-sensitive genic male sterility(P/TGMS) lines in China remains unclear. Using 48 simple sequence repeat(SSR) markers, we genotyped a panel of 208 indica P/TGMS lines and confirmed three subgroups, named indica-I, indica-II and indica-III, in indica P/TGMS lines. Further diversity analysis indicated indica-II had the highest genetic diversity. The genetic differentiation between indica-II and indica-III was demonstrated as the largest among the three subgroups. Moreover, indica/japonica component identification was detected that five P/TGMS lines possess indica components less than 0.900. These results improve our knowledge on the genetic background for P/TGMS lines in China and will be beneficial for hybrid rice breeding programs. | ZHANG Mengchen WANG Shan YAN Jianfang SUN Shuiyong XU Xin XU Qun YUAN Xiaoping WEI Xinghua YANG Yaolong | 2019 | Rice science2019，26，3： | 0 |
| 11 | Electrostatic and hydrophobic interaction cooperative nanochaperone regulates protein folding显示文摘Natural molecular chaperones utilize spatially ordered multiple molecular forces to effectively regulate protein folding.However,synthesis of such molecules is a big challenge.The concept of“aggregate science”provides insights to construct chemical entities(aggregates)beyond molecular levels to mimic both the structure and function of natural chaperone.Inspired by this concept,herein we fabricate a novel multi-interaction(i.e.,electrostatic and hydrophobic interaction)cooperative nanochaperone(multi-co-nChap)to regulating protein folding.This multi-co-nChap is fabricated by rationally introducing electrostatic interactions to the surface(corona)and confined hydrophobic microdomains(shell)of traditional single-hydrophobic interaction nanochaperone.We demonstrate that the corona electrostatic attraction facilitates the diffusion of clients into the hydrophobic microdomains,while the shell electrostatic interaction balances the capture and release of clients.By finely synergizing corona electrostatic attraction with shell electrostatic repulsion and hydrophobic interaction,the optimized multi-co-nChap effectively facilitated de novo folding of nascent polypeptides.Moreover,the synergy between corona electrostatic attraction,shell electrostatic attraction and shell hydrophobic interaction significantly enhanced the capability of multi-co-nChap to protect native proteins from denaturation at harsh temperatures.This work provides important insights for understanding and design of nanochaperone,which is a kind of ordered aggregate with chaperone-like activity that beyond the level of single molecule. | Xiaohui Wu Fei Deng Yujie Chen Mengchen Xu Feihe Ma Linqi Shi | 2024 | Aggregate2024，5，1： | 0 |
| 12 | Rare and undiagnosed diseases:From disease-causing gene identification to mechanism elucidation显示文摘Rare and undiagnosed diseases substantially decrease patient quality of life and have increasingly become a heavy burden on healthcare systems.Because of the challenges in disease-causing gene identification and mechanism elucidation,patients are often confronted with difficulty obtaining a precise diagnosis and treatment.Due to advances in sequencing and multiomics analysis approaches combined with patient-derived iPSC models and gene-editing platforms,substantial progress has been made in the diagnosis and treatment of rare and undiagnosed diseases.The aforementioned techniques also provide an operational basis for future precision medicine studies.In this review,we summarize recent progress in identifying disease-causing genes based on GWAS/WES/WGS-guided multiomics analysis approaches.In addition,we discuss recent advances in the elucidation of pathogenic mechanisms and treatment of diseases with state-of-the-art iPSC and organoid models,which are improved by cell maturation level and gene editing technology.The comprehensive strategies described above will generate a new paradigm of disease classification that will significantly promote the precision and efficiency of diagnosis and treatment for rare and undiagnosed diseases. | Gang Wang Yuyan Xu Qintao Wang Yi Chai Xiangwei Sun Fan Yang Jian Zhang Mengchen Wu Xufeng Liao Xiaomin Yu Xin Sheng Zhihong Liu Jin Zhang | 2022 | Fundamental Research2022，2，6： | 0 |
| 13 | Antioxidant defense mechanisms and fatty acid catabolism in Red-billed Leiothrix(Leiothrix lutea)exposed to high temperatures显示文摘Extreme hot weather is occurring more frequently due to global warming,posing a significant threat to species survival.Birds in particular are more likely to overheat in hot weather because they have a higher body temperature.This study used a heat stress model to investigate the antioxidant defense mechanisms and changes in fatty acid catabolism in Red-billed Leiothrix(Leiothrix lutea)to gain an understanding of how birds adapt to high temperatures.The birds were divided into five groups:a control group(30℃for 0 days),1 D group(40℃for 1 day),3 D group(40℃for 3 days),14 D group(40℃for 14 days)and recovery group(40℃for 14 days,then 30℃for 14 days).Our results indicated that when Red-billed Leiothrix are subjected to heat stress,malondialdehyde(MDA)content in the liver significantly increased,as did the enzyme activities of catalase(CAT),glutathione-SH-peroxidase(GSH-PX)and total antioxidant capacity(T-AOC)in the liver.Furthermore,there was a significant increase in heat shock protein 70(HSP70)expression in the liver,while avian uncoupling protein(avUCP)expression in muscle was significantly reduced.Additionally,there was a significant reduction in fatty acid catabolism enzyme activity such as 3-hydroxyacyl-CoAdehydrogenase(HOAD)activity in the heart,and carnitine palmitoyl transferase 1(CPT-1)and citrate synthase(CS)activity in the heart and liver.Furthermore,fatty acid translocase(FAT/CD36)in the heart,heart-type fatty acid binding protein(H-FABP)and fatty acid binding protein(FABP-pm)in the liver and heart were also significantly decreased.These changes reverted after treatment,but not to the same level as the control group.Our results indicated that when Red-billed Leiothrix are exposed to heat stress their internal antioxidant defense system is activated to counteract the damage caused by high temperatures.However,even with high antioxidant levels,prolonged high temperature exposure still caused some degree of oxidative damage possibly requiring a longer recovery time.Additionally,Red-billed Leiothrix may be able to resist heat stress by reducing fatty acid transport and catabolism. | Ruiping Xu Canwen Yu Liyao Mao Mengchen Jiang Luyao Gao Ming Li Jinsong Liu | 2022 | Avian Research2022，13，1： | 0 |
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