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| 1 | GPS-constrained inversion of present-day slip rates along major faults of the Sichuan-Yunnan region, China显示文摘A linked-fault-element model is employed to invert for contemporary slip rates along major active faults in the Sichuan-Yunnan region (96°-108°E, 21°-35°N) using the least squares method. The model is based on known fault geometry, and constrained by a GPS-derived horizontal velocity field. Our results support a model attributing the eastward extrusion of the Tibetan Plateau driven mainly by the north-northeastward indentation of the Indian plate into Tibet and the gravitational collapse of the plateau. Resisted by a relatively stable south China block, materials of the Sichuan-Yunnan region rotate clockwise around the eastern Himalayan tectonic syntaxis. During the process the Garzê-Yushu, Xianshuihe, Anninghe, Zemuhe, Daliangshan, and Xiaojiang faults, the southwest extension of the Xiaojiang fault, and the Daluo-Jinghong and Mae Chan faults constitute the northeast and east boundaries of the eastward extrusion, with their left slip rates being 0.3-14.7, 8.9-17.1, 5.1 ± 2.5, 2.8 ± 2.3, 7.1 ± 2.1, 9.4 ± 1.2, 10.1 ± 2.0, 7.3 ± 2.6, and 4.9 ± 3.0 mm/a respectively. The southwestern boundary consists of a widely distributed dextral transpressional zone other than a single fault. Right slip rates of 4.2 ± 1.3, 4.3 ± 1.1, and 8.5 ± 1.7 mm/a are detected across the Nanhua-Chuxiong-Jianshui, Wuliangshan, and Longling-Lancang faults. Crustal deformation across the Longmenshan fault is weak, with short-ening rates of 1.4 ± 1.0 and 1.6 ± 1.3 mm/a across the Baoxing-Beichuan and Beichuan-Qingchuan segments. Northwest of the Longmenshan fault lies an active deformation zone (the Longriba fault) with 5.1±1.2 mm/a right slip across. Relatively large slip rates are detected across a few faults within the Sichuan-Yunnan block: 4.4±1.3 mm/a left slip and 2.7±1.1 mm/a shortening across the Litang fault, and 2.7±2.3 mm/a right-lateral shearing and 6.7±2.3 mm/a shortening across the Yunongxi fault and its surrounding regions. In conclusion, we find that the Sichuan-Yunnan region is divided into more than a dozen active micro-blocks by a large number of faults with relatively slow slip rates. The eastward extrusion of the Tibetan Plateau is absorbed and adjusted in the region mainly by these faults, other than a small number of large strike-slip faults with fast slip rates. | WANG YanZhao WANG EnNing SHEN ZhengKang WANG Min GAN WeiJun QIAO XueJun MENG GuoJie LI TieMing TAO Wei YANG YongLin CHENG Jia LI Peng | 2008 | Science China Earth Sciences2008，51，9： | 65 |
| 2 | Permian volcanisms in eastern and southeastern margins of the Jiamusi Massif, northeastern China: zircon U-Pb chronology, geochemistry and its tectonic implications显示文摘LA-ICP-MS zircon U-Pb dating and geochemical data for the Late Paleozoic volcanic rocks from eastern and southeastern margins of the Jiamusi Massif are presented to understand the regional tectonic evolution. Zircons from eight representative volcanic rocks are euhedral-subhedral in shape and dis- play striped absorption and fine-scale oscillatory growth zoning as well as high Th/U ratios (0.33―2.37), implying a magmatic origin. The dating results show that the Late Paleozoic volcanic rocks in the study area can be divided into two stages, i.e., the Early Permian (a weighted mean 206Pb/238U age of 288 Ma) and the Middle Permian volcanisms (a weighted mean 206Pb/238U age of 268 Ma). The former is com- posed mainly of basalt, basaltic-andesite, andesite and minor dacite. They are characterized by low SiO2 contents, high Mg# (0.40―0.59), enrichment in Na (Na2O/K2O = 1.26―4.25) and light rare earth elements (LREEs), relative depletion in heavy rare earth elements (HREEs) and high field strength ele- ments (HFSEs), indicating that an active continental margin setting could exist in the eastern margin of the Jiamusi Massif in the Early Permian. The latter consists mainly of rhyolite and minor dacite with high SiO2 (77.23%―77.52%), low MgO (0.11%―0.14%), enrichment in K2O (Na2O/K2O ratios < 0.80) and Rb, Th, U and depletion in Eu, Sr, P and Ti, implying a crust-derived origin. Therefore, it is proposed that the Middle Permian volcanic rocks could have formed under the collision of the Jiamusi and the Khanka Massifs. | MENG En XU WenLiang YANG DeBin PEI FuPing Yu Yang Zhang XingZhou | 2008 | Chinese Science Bulletin2008，53，8： | 42 |
| 3 | Boundedness of Some Maximal Commutators in Hardy-type Spaces with Non-doubling Measures显示文摘让μ是满足一些仅仅生长条件的 R^d 上的一项非否定的氡措施。在这个假设下面,在一些强壮类型的空格的围住的海角为是 Calderon-Zygmund 操作符和 RBMO 产生的平常的最大的整流器的变体的最大的 Calderon-Zygmund 操作符和最大的整流器的一个类被建立(μ)函数,在强壮类型的空格是一些的地方,拨出潜水艇空格,与考虑 RBMO 联系了(μ)函数,强壮的空格 H^1 ( Tolsa 的μ)。 | Guo En HU Yan MENG Da Chun YANG | 2007 | Acta Mathematica Sinica,English Series2007，23，6： | 8 |
| 4 | A New Discovery of ~2.7 Ga Granitic Magmatism in Southeastern Jilin Province, China显示文摘Objective The North China Craton(NCC)is characterized by widespread tectono-thermal events at ca.2.5 Ga,which differs from many other older cratons on Earth,which usually record tectono-thermal events at-2.7 Ga.Therefore,the search for early crustal material,especially rocks within the NCC that record the 2.7 Ga event,is | WANG Chaoyang MENG En LI Yanguang CAI Jia YANG Hong LI Zhuang | 2017 | Acta Geologica Sinica(English Edition)2017，91，5： | 5 |
| 5 | 北斗导航系统卫星激光测距综述显示文摘Satellite laser ranging(SLR)is an unambiguous measurement technique and generates high accuracy satellite orbit data.All satellites in the BeiDou navigation satellite system(BDS)carried laser retro-reflector arrays(LRAs),so they can be tracked by ground SLR stations in order to provide the accurate observation data.The Shanghai astronomical observatory(SHAO)designed the LRAs,and also developed the dedicated SLR systems using a 1 m-aperture telescope and a transportable cabin-based SLR system with a telescopes of 60 cm aperture.These enable BDS satellite ranging during daytime and nighttime with centimeter-level precision,allowing highly accurate estimations of satellite orbits.Moreover,some of the BDS satellites are also equipped with laser time transfer(LTT)payloads,which were developed by the SHAO and China Academy of Space Technology(CAST),providing a highly accurate time comparison between the satellites and ground clocks.This paper describes the dedicated SLR system and the design of the LRAs for BDS satellites,as well as global SLR measurements.The SLR tracking data is used for evaluating the orbit accuracy of BDS satellites and broadcast ephemeris,with an accuracy of less than 1 m.The LTT measurements to BDS satellites for a single shot have a precision of approximately 300 picoseconds,with a time stability of 20 picoseconds in 500 s. | ZHANG Haifeng LONG Mingliang YANG Huafeng MENG Wendong CHENG Zhi’en DENG Huarong ZHANG Zhongping ZHANG A’li | 2020 | Aerospace China2020，21，4： | 4 |
| 6 | Building Osteogenic Microenvironments with a Double-Network Composite Hydrogel for Bone Repair显示文摘The critical factor determining the in vivo effect of bone repair materials is the microenvironment,which greatly depends on their abilities to promote vascularization and bone formation.However,implant materials are far from ideal candidates for guiding bone regeneration due to their deficient angiogenic and osteogenic microenvironments.Herein,a double-network composite hydrogel combining vascular endothelial growth factor(VEGF)-mimetic peptide with hydroxyapatite(HA)precursor was developed to build an osteogenic microenvironment for bone repair.The hydrogel was prepared by mixing acrylatedβ-cyclodextrins and octacalcium phosphate(OCP),an HA precursor,with gelatin solution,followed by ultraviolet photo-crosslinking.To improve the angiogenic potential of the hydrogel,QK,a VEGF-mimicking peptide,was loaded in acrylatedβ-cyclodextrins.The QK-loaded hydrogel promoted tube formation of human umbilical vein endothelial cells and upregulated the expression of angiogenesis-related genes,such as Flt1,Kdr,and VEGF,in bone marrow mesenchymal stem cells.Moreover,QK could recruit bone marrow mesenchymal stem cells.Furthermore,OCP in the composite hydrogel could be transformed into HA and release calcium ions facilitating bone regeneration.The double-network composite hydrogel integrated QK and OCP showed obvious osteoinductive activity.The results of animal experiments showed that the composite hydrogel enhanced bone regeneration in skull defects of rats,due to perfect synergistic effects of QK and OCP on vascularized bone regeneration.In summary,improving the angiogenic and osteogenic microenvironments by our double-network composite hydrogel shows promising prospects for bone repair. | Jiaying Li Jinjin Ma Qian Feng En Xie Qingchen Meng Wenmiao Shu Junxi Wu Liming Bian Fengxuan Han Bin Li | 2023 | Research2023，，3： | 3 |
| 7 | Upwelling Process of the Western Himalaya Mountains:Height and Velocity Estimation Evidenced by Formation and Evolution of the Zanda Basin,Tibet,China显示文摘把地质的调查, stratigraphic 节测量和室内的全面调查基于地, Zanda 盆在 Himalaya 板中的构造地点被查明,并且 Zanda 盆的形成和进化作为六个阶段在到早更新世的上新世期间被分类:(a) 主要指责裂缝的舞台,(b) 快指责裂缝的舞台,(c) 集中的指责裂缝的舞台,(d) 壅滞舞台,(e) 第二等的指责裂缝的舞台,和(f) 第二等的快指责裂缝的舞台。基于 Zanda 盆的这个六阶段的形成进化理论,当下列五上演,到早更新世的从上新世的西方的 Himalaya 山的 upwelling 过程被分类:(a) 慢 upwelling 舞台(5.44.4 妈) ,(b) 中间速度的 upwelling 舞台(4.43.5 妈) ,(c) 快 upwelling 舞台(3.53.2 妈) ,(d)停止 upwelling 阶段(3.22.7 妈) ,和(e) 快 upwelling 舞台(2.7 妈) 。研究从早上新世(4.7 妈) 在持续时间显示出那到上新世(2.67 妈) 的目的,它持续了 203 万年, Himalaya 山高举了在 0.74 mm/a 的速度的 1500 m;这属于中间速度的 upwelling。在在早更新世的早阶段的 131 万年期间, Himalaya 山在 1.15 mm/a 的速度在另一 1500 m 上面升起了;这是相当快的 upwelling。所有这些数据证明了西方的 Himalaya 山的 upwelling 沿着有 multi-stages,多速度,和非天律不变论的特征的一个复杂过程。 | ZHU Dagang MENG Xian' gang SHAO Zhaogang YANG Chaobin HAN Jian'en YU Jia MENG Qingwei Lü Rongping | 2010 | Acta Geologica Sinica(English Edition)2010，84，2： | 3 |
| 8 | Zircon U-Pb age,geochemical and Nd isotopic data of Middle Jurassic high-Mg dioritic dike in Liaodong Peninsula,NE China显示文摘Zircon LA-ICP-MS U-Pb age,geochemical and Nd isotopic data are presented for a newly recognized high-Mg dioritic dike from Haicheng,Liaodong Peninsula,NE China,to constrain its petrogenesis. The zircons from the high-Mg diorite exhibit striped absorption and oscillatory growth zoning in the cathodoluminescence( CL) images,and have high Th/U ratios( 0. 05--0. 9),indicating a magma origin. Zircon LA-ICP-MS U-Pb dating indicates that206 Pb /238 U ages of 12 spots of zircons are between 167 Ma and 178 Ma,yielding a weighted mean206 Pb /238 U age of 172 ± 2 Ma( MSWD = 4. 1),which represents the forming age of the high-Mg dioritic dike,i. e. Middle Jurassic. Geochemically,the samples have SiO2= 55. 4--60. 6 wt. %,Na2 O = 2. 2--2. 76 wt. %,K2 O = 1. 32--2. 02 wt. % and( Na2 O + K2O) = 3. 82--4. 47 wt. %,belonging to sub-alkaline series and displaying a calc-alkaline evolutionary trend. They are characterized by high MgO( 4. 75--6. 85 wt. %),Mg#( 55--61),Cr( 130--262 ppm),Ni( 63--130 ppm),Sr( 568--857 ppm),and Ba( 484--1 130 ppm) contents,with geochemical features analogous to those of high-Mg adakites. They show variable εNd( t) values(-1. 3 to- 3. 9),with a weighted value of- 2. 7,which plot intermediately between the field of the ancient continental crust and the depleted mantle source,indicating that both the lower crust and mantle source are necessary for the generation of the parent magma of the Haicheng high-Mg diorites. The Haicheng high-Mg dioritic dike in the Liaodong Peninsula and the Jurassic magmatism in the eastern North China Craton formed under a continental crustal thickening setting that may be related to subduction of the Paleo-Pacific oceanic plate. | LI Zhuang PEI Fuping MENG En | 2014 | Global Geology2014，17，3： | 3 |
| 9 | A new chain-like heteropolytungstate formed by Keggin cluster units:Synthesis and structure of[H2bpy]3[SiMnW(11)O(39)].1.25H2O显示文摘新 heteropolytungstate,[H2bpy ] 3 [SiMnW11O39 ]· 1 .25H2O (1 ) ,在温和热水的条件下面被准备了,在结构上由单晶 X 射线衍射描绘了。包含过渡金属元素的 1D 曲折链是首先描绘的化合物的代替了 Keggin heteropolytungstate,它通过一个普通的氧原子被连接。它的元素的分析和红外也被描述。 | Jing Xin Meng Ying Ma Xin Xin Xu Yang Guang Li En Bo Wang | 2007 | Chinese Chemical Letters2007，18，1： | 2 |
| 10 | Multilinear Calderón-Zygmund Operator on Products of Hardy Spaces显示文摘In this paper, the authors establish the boundedness of the multilinear Calderón-Zygmund operator from products of Hardy spaces into Hardy spaces. | Guo En HU Yan MENG | 2012 | Acta Mathematica Sinica,English Series2012，28，2： | 1 |
| 11 | Down‐regulated desmocollin‐2 promotes cell aggressiveness through redistributing adherens junctions and activating beta‐catenin signalling in oesophageal squamous cell carcinoma显示文摘 | Wang‐Kai Fang Lian‐Di Liao Li‐Yan Li Yang‐Min Xie Xiu‐E Xu Wei‐Jiang Zhao Jian‐Yi Wu Meng‐Xiao Zhu Zhi‐Yong Wu Ze‐Peng Du Bing‐Li Wu Dong Xie Ming‐Zhou Guo Li‐Yan Xu En‐Min Li | 2013 | J Pathol2013，，2： | 1 |
| 12 | Superior performance of a 2 kHz pulse Nd:YAG laser based on a gradient-doped crystal显示文摘Herein,we report a homemade new Nd:YAG crystal rod that contains a gradient dopant of 0.39–0.80 at.%Nd3+ from end to end,achieving superior performance of a 2 kHz Nd:YAG pulse laser at 1064 nm. The optical-tooptical conversion efficiency reached 53.8%,and the maximum output power of the laser was 24.2 W,enhanced by 35.9% compared with a uniform crystal rod with the same total concentration of Nd3+. Significantly,our experiments revealed that the gradient concentration crystal produced a relatively even pumping distribution along the rod axis,greatly reducing the temperature gradient as well as having a smaller thermal effect. The pump and thermal distribution smoothing obviously improved the features of laser oscillation and output. | MENG’EN WEI TINGQING CHENG RENQIN DOU QINGLI ZHANG HAIHE JIANG | 2021 | Photonics Research2021，9，7： | 1 |
| 13 | Involvement of Purinergic Neurotransmission in Ketamine Induced Bladder Dysfunction显示文摘 | En Meng Han-Yu Chang Sun-Yran Chang Guang-Huan Sun Dah-Shyong Yu Tai-Lung Cha | 2011 | The Journal of Urology2011，，3： | 1 |
| 14 | Sim- ultaneous determination of geniposide, chlorogenic acid, crocin - I , and rutin in crude and processed Fructus Gardeniae extracts by high performance liquid chromatography 显示文摘 | OUYANG En - hong ZHANG Cheng - rong LI Xi - meng | 2011 | Pharmacogn Mag2011，7，28： | 1 |
| 15 | Definition of the Quaternary Qiangtang Paleolake in Qinghai-Tibetan Plateau, China显示文摘Since the Quaternary, many lakes have been present in the Qinghai-Tibetan Plateau. As peculiar geological processes in the evolution of the uplifting of Qinghai-Tibetan Plateau, the distributions and evolutions of the Quaternary paleolakes in the Qinghai-Tibetan Plateau have been the focus of interest among the international geosciences circle. Comparisons of the newly obtained and existing data from field surveys, remote sensing images, characteristics of tectonic landforms and distribution of the lacustrine strata, the author have, for the first time, defined a large-sized Quaternary Qiangtang Paleolake. The paleolake starts from the east-westerly direction at Rutog in western Tibet, passing through Gêrzê, and finally ends at Nagqu in eastern Tibet. Its length is approximately 1,200 km; it is about 420 km at its widest point (north-southerly). The Paleolake forms an E-W (or NWW) ellipsoid with an estimated area of 354920 km2 . The Paleolake is bordered by the Mts. Gangdisê and Nyainqêntanglha to the south and the Karakorum Pass-Tozê Kangri-Zangbagangri-Tanggula Pass to the north. It generally appears as a basinal landform with low mountains and valleys in the central part (altitudes of 4400 m) and higher altitudes (5000 m) in the peripheries. The formation and development of the Paleolake was controlled by the nearly E-W trending structures. There are three east-westerly extending tectonic sutures inside the Paleolake area, from north to south: (1) the Shuanghu Suture; (2) the Bangong Co-Nujiang River Suture; (3) the Shiquanhe-Lhari Suture. These three sutures have generally controlled the spreading features of the Paleolake and act as the first grade lake-controlling structures. In the southern Paleolake basin, there are a series of south-northerly rift basins, which are controlled by the normal faults and exist as a series of south-northerly grabens and semi-graben faulted basins. The south-northerly rift zones have clearly exerted control over the south margin of the Paleolake as well as the distribution of the residual lake basins after the Paleolake's break up. They are the secondary lake-controlling tectonics. Discoveries of the lacustrine strata inside the Plateau, especially the successive discoveries of many high-stand lacustrine strata, are direct evidence supporting the existence of the paleolakes in the Plateau. The dating results of the lake-eroded travertine in the Dong Co in the hinterland of the Quaternary Qiangtang Paleolake have revealed that the lake-eroded travertine is of Late Pleistocene sediments. By considering the analysis of the lacustrine strata as well, the researchers have revealed that the Quaternary Qiangtang Paleolake begins its embryonic form in the Early Pleistocene, successively develops till the Middle Pleistocene, and reaches its full blossom in the middle stage of the Late Pleistocene; it further shrinks and separates by the end of the Late Pleistocene, and finally ceases its whole life as the paleolake. After the Holocene, the huge Late Pleistocene Qiangtang Paleolake no longer exists, leaving a series of widely distributed smaller lakes on the Qiangtang Plateau. These smaller, isolated water systems receive the Holocene lacustrine sediments. | SHAO Zhaogang MENG Xian'gang HAN Jian'en ZHU Dagang YANG Chaobin WANG Jin YU Jia WANG Yan HE Chengguang | 2013 | Acta Geologica Sinica(English Edition)2013，87，2： | 1 |
| 16 | Response of the xenograft endothelium in the concordant xenotransplantation显示文摘Objective: To investigate the response of the xenograft endothelium in the concordant hamster to rat cardiac xenotransplantation and the mechanism of acute vascular rejection. Methods: The animals were divided into 5 groups randomly: control group, CsA group, splenectomy group, D0 splenectomy+CsA group and D3 splenectomy+CsA group. Hamster heart was heterotopicaly transplanted to rat abdominal cavity. The graft survival was monitored by palpation of the rat abdominal wall. The histological and ultrastructural changes of the xenogafts were investigated. NF-κB and P-selectin expression in the xenograft were detected. Heme Oxigenase-1 and Bcl-2 expression were also detected in the xenografts of different groups. Results: The mean survival time of the xenografts in control group,CsA group, splenectomy group, D0 splenectomy+CsA group and D3 splenectomy+CsA group was 3.4 ± 0.55, 3.8 ± 0.45, 6.4 ± 1.52, 30 and 7.4 ± 1.14 days. The rejected graft showed typical acute vascular rejection in control group, CsA group,splenectomy group and D3 splenectomy+CsA group. Endothelial cells of the rejected xenograft showed dramatic assembly of ribosomes and expansion of the rough endoplasmic reticulum. However, the endothelium of the long-term survived grafts in D0 splenectomy+CsA group showed normal architecture. NF-κB and P-selectin expression were detected in the rejected xenografts. HO-1 expression was observed in the long-term survived xenografts in D0 splenectomy+CsA group. Conclusion: The endothelial cells of the xenograft might be activated during the acute vascular rejection. Expression of HO-1 might inhibit the upregulation of NF-κB and adhesion molecular which decreases the activation of the endothelium of the graft. | Bo Wang Yi Lu Cheng' en Pan Xiaogang Zhang Hui Li Kewei Meng ZhengWu | 2007 | Journal of Nanjing Medical University2007，21，1： | 1 |
| 17 | Investigation and Design of General Seismic Data Model Based on Relational Database显示文摘 | Meng En Meng Dong-yue Wang Hon-gwei Zhao Guang-tao | 2009 | 石油地球物理勘探2009，44，A02： | 0 |
| 18 | Unusual presentation of penile giant condyloma acuminatum with spontaneous prepuce perforation: A case report显示文摘BACKGROUND Giant condyloma acuminatum(GCA)is an uncommon condition affecting the anogenital area.CASE SUMMARY Here,we report an 88-year-old male patient who presented with a 5-year history of a progressive appearance of multiple cauliflower-like warts over his penile shaft,pubis region,and groin as well as urine leakage along the distal penile shaft.Physical examination revealed an ulcerative skin perforation with pus discharge under the distal prepuce base,which was initially suspected to be a urethral fistula.However,during surgery,it was discovered that the perforation was caused by a giant condyloma lesion that had obliterated the prepuce opening,with infection and high pressure causing subsequent skin perforation.He underwent circumcision and wide excision with electrocauterization of the warts.He was discharged after the surgery,and the residual lesion was treated with imiquimod and low-dose oral tegafur-uracil.CONCLUSION Penile GCA can cause prepuce perforation and can be postoperatively treated with imiquimod and low-dose oral tegafur-uracil. | Fu-Chieh Hsu Dah-Shyong Yu Ta-Wei Pu Min-Jui Wu En Meng | 2023 | World Journal of Clinical Cases2023，11，29： | 0 |
| 19 | Electrochemical quartz crystal microbalance study on Au-supported Pt adlayers for electrocatalytic oxidation of methanol in alkaline solution显示文摘Underpotential deposition(UPD) of Cu on an Au electrode followed by redox replacement reaction(RRR) of CuUPD with a Pt source(H2PtCl6 or K2PtCl4) yielded Au-supported Pt adlayers(for short,Pt(CuUPD-Pt4+)n/Au for H2PtCl6,or Pt(CuUPD-Pt2+)n/Au for K2PtCl4,where n denotes the number of UPD-redox replacement cycles).The electrochemical quartz crystal microbalance(EQCM) technique was used for the first time to quantitatively study the fabricated electrodes and estimate their mass-normalized specific electrocatalytic activity(SECA) for methanol oxidation in alkaline solution.In comparison with Pt(CuUPD-Pt2+)n/Au,Pt(CuUPD-Pt4+)n/Au exhibited a higher electrocatalytic activity,and the maximum SECA was obtained to be as high as 35.7 mA ?g?1 at Pt(CuUPD-Pt4+)3/Au.The layer-by-layer architecture of Pt atoms on Au is briefly discussed based on the EQCM-revealed redox replacement efficiency,and the calculated distribution percentages of bare Au sites agree with the experimental results deduced from the charge under the AuOx-reduction peaks.The EQCM is highly recommended as an efficient technique to quantitatively examine various electrode-supported catalyst adlayers,and the highly efficient catalyst adlayers of noble metals are promising in electrocatalysis relevant to biological,energy and environmental sciences and technologies. | HUANG Zhao,JIA Xue’En,XIE QingJi*,TAN YueMing,HUANG SuQing,HUANG JinHua,MENG Yue & YAO ShouZhuo* Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research,Ministry of Education College of Chemistry and Chemical Engineering,Hunan Normal University,Changsha 410081,China | 2010 | Science China Chemistry2010，53，11： | 0 |
| 20 | No effect of exogenous melatonin on development of cryopreserved metaphase Ⅱ oocytes in mouse显示文摘Background:This study was conducted to investigate effect of exogenous melatonin on the development of mouse mature oocytes after cryopreservation.Results:First,mouse metaphase Ⅱ(MII) oocytes were vitrified in the open-pulled straws(OPS).After warming,they were cultured for 1 h in M_2 medium containing melatonin at different concentrations(0,10^(-9),10^(-7),10^(-5),10^(-3)mol/L).Then the oocytes were used to detect reactive oxygen species(ROS) and glutathione(GSH) levels(fluorescence microscopy),and the developmental potential after parthenogenetic activation.The experimental results showed that the ROS level and cleavage rate in 10^(-3) mol/L melatonin group was significantly lower than that in melatonin-free group(control).The GSH levels and blastocyst rates in all melatonin-treated groups were similar to that in control.Based on the above results,we detected the expression of gene Hsp90aa1,Hsf1,Hspa1 b,Nrf2 and Bcl-x1 with qRT-PCR in oocytes treated with 10^(-7),or 10^(-3) mol/L melatonin and untreated control.After warming and culture for 1 h,the oocytes showed higher Hsp90aa1 expression in 10^(-7) mol/L melatonin-treated group than in the control(P<0.05);the Hsf1,Hsp90aa1 and Bcl-x1 expression were significantly decreased in 10^(-3) mol/L melatonin-treated group when compared to the control.Based on the above results and previous research,we detected the development of vitrified-warmed oocytes treated with either 10^(-7) or 0 mol/L melatonin by in vitro fertilization.No difference was observed between them.Conclusions:Our results indicate that the supplementation of melatonin(10^(-9) to 10^(-3) mol/L) in culture medium and incubation for 1 h did not improve the subsequent developmental potential of vitrified-warmed mouse MII oocytes,even if there were alteration in gene expression. | Wei Li Keren Cheng Yue Zhang Qinggang Meng Shi'en Zhu Guangbin Zhou | 2015 | Journal of Animal Science and Biotechnology2015，6，4： | 0 |
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