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| 1 | A simple inorganic-solvent-thermal route to nanocrystalline niobium diboride显示文摘 | Jianhua Ma Yihong Du Meining Wu Guoxing Li Zhiqing Feng Ming Guo Yixiu Sun Weihua Song Mingjin Lin Xule Guo | 2008 | Journal of Alloys and Compounds2008，，： | 1 |
| 2 | Single-atom electrocatalysis: a new approach to in vivo electrochemical biosensing显示文摘Modulation of interfacial electron transfer has been proven to pave a new approach to in vivo electrochemical monitoring of brain chemistry;however,designing and establishing highly efficient electrocatalytic scheme towards neurochemicals remain a longstanding challenge.Here,we find that recently established single-atom catalyst(SAC)can be used for catalyzing the electrochemical process of physiologically relevant chemicals and thus offers a new avenue to in vivo electrochemical biosensing.To prove this new concept,we used Co single-atom catalyst(Co-SAC),in which the atomic active sites are dispersed in ordered porous N-doping carbon matrix at atomic level,as an example of SACs for analyzing glucose as the physiologically relevant model chemicals.We found that Co-SAC catalyzes the electrochemical oxidation of hydrogen peroxide(H2O2)at a low potential of ca.+0.05 V(vs.Ag/AgCl).This property was further used for developing an oxidase-based glucose biosensor that was used subsequently as a selective detector of an online electrochemical system(OECS)for continuous monitoring of microdialysate glucose in rat brain.The OECS with Co-SAC-based glucose biosensor as the online detector was well responsive to glucose without interference from other electroactive species in brain microdialysate.This study essentially offers a new approach to in vivo electrochemical analysis with SACs as electrocatalysts to modulate interfacial electron transfer. | Hanfeng Hou Junjie Mao Yunhu Han Fei Wu Meining Zhang Dingsheng Wang Lanqun Mao Yadong Li | 2019 | Science China Chemistry2019，62，12： | 1 |
| 3 | Choriocarcinoma-associated pulmonary thromboembolism and pulmonary hypertension: a case report显示文摘Cases of pulmonary embolism and pulmonary artery hypertension caused by choriocarcinoma represent a rare clinical emergency.We report a case of a 25-year-old woman who presented with pulmonary embolism and hypertension and died soon after complete pulmonary embolectomy.A related literature review revealed that almost all of these patients had previously experienced a spontaneous abortion(average,6 months) and were not pregnant. | Yan Zhu Meining Yu Luyao Ma Hai Xu Fanghong Rose Li | 2016 | The Journal of Biomedical Research2016，30，3： | 1 |
| 4 | 沉默Sp1抑制大肠癌细胞SW480的端粒酶活性并促进细胞凋亡(英文)显示文摘Objective:The aim of the study was to examine the effect of Sp1 on the expression of the human telomerase reverse transcriptase(hTERT) gene in human colorectal carcinoma SW480 cells.Methods:The Sp1 shRNA plasmid was transfected into colorectal carcinoma SW480 cells line by liposome mediation for transient expression.After Sp1 shRNA plasmid transfected SW480 cells,the exogenous Sp1 protein expression was determined by the method of Western blot.At same time,hTERT mRNA expression was detected by RT-PCR,telomerase activity was determined by the telomeric repeat amplification protocol(TRAP) assay,and the apoptotic rate of cells was also tested by flow cytometry.Results:The protein expressions of Sp1 gene could be reduce by transfecting of pGenesil-1-Sp1(+) recombinant plasmid into SW480 cells.The apoptotic rate was increased compared with pGenesil-1-Sp1(-)/SW480 and SW480(P < 0.05),which indicated that lowexpression of Sp1 gene could lead to low level of telomerase activity and induce apoptosis.Conclusion:Silencing Sp1 may suppress the activity of telomerase by inhabiting hTERT gene expression. | Liguo Zhao Yan Zhu Wantong Niu Meining Li Niuliang Cheng | 2011 | The Chinese-German Journal of Clinical Oncology2011，10，4： | 1 |
| 5 | Silk fibroin layer-by-layer microcapsules for localized gene delivery显示文摘 | Li L H Puhl S Meine! L | 2014 | Biomaterials2014，35，： | 1 |
| 6 | 降低Pin1对大肠癌SW620细胞的增殖和凋亡能力的影响(英文)显示文摘Objective:The aim of our study was to investigate the effects of Pin1 reduction on SW620 cell proliferation and apoptosis in human colorectal carcinoma.Methods:We constructed a plasmid of RNA interfering(shRNA) for Pin1 gene(pGenesl-1-Pin1),then the plasmid was transfected into colorectal carcinoma SW620 cells line by liposome mediation.The protein expression of Pin1 was tested by Western blotting.The proliferation rate was analyzed by MTT and the apoptotic rate of cells was tested by flow cytometry.In order to explain further the effect of Pin1 in SW620 cells,the protein level of Bcl-2 was analyzed by Western blotting.Results:pGenesil-1-Pin1 plasmid was successfully constructed and confirmed by sequencing.The protein relative levels of Pin1 were 0.06 ± 0.04 for the P-shRNA/SW620 cells,and 0.32 ± 0.09 for the P-Con/SW620 cells.The cell growth rate of SW620 cells was slower while the apoptotic rate was increased after transfection with pGenesil-Pin1 plasmid,and the apoptotic rate was 12.38% ± 1.55% for the P-shRNA/SW620 group.At the same time,we found that the protein expression of Bcl-2 was also reduced.The results were 0.13 ± 0.04 for the P-shRNA/SW620 cells,and 0.36 ± 0.08 for the P-Con/SW620 cells.Conclusion:Inhibited Pin1 expression may suppress the cell proliferation and promote apoptosis of colorectal carcinoma cells in vitro. | Yan Zhu Liyuan Qin Meining Li Dong Zhang Yuehong Zhang Niuliang Cheng | 2011 | The Chinese-German Journal of Clinical Oncology2011，10，2： | 0 |
| 7 | Nkx2.1 downregulation is involved in brain abnormality induced by excess retinoic acid显示文摘Abnormal development of central nervous system(CNS)caused by neural tube defects is not only a major con tributor in the prevale nee of stillbirths and n eonatal deaths but also causes lifelong physical disability in surviving infants.Due to insufficient known investigated causes,CNS developmental abnormality has brought sever burden on health around the world.From previous results of high throughput transcriptome sequencing,we selected transcription factor Nkx2.1 as a candidate to investigate its role on brain abnormalities induced by excessive retinoic acid.The result of in situ hybridization showed that Nkx2.1 was mainly expressed in mouse brain.After the Nkx2.1 gene was sileneed,retarded proliteration and accelerated apoptosis were found in mouse Neuro-2a(N2a)cells.Furthermore,our results indicated that the main components of sonic hedgehog(Shh)signaling pathway were affected in Nkx2.7-silenced cells,implying that Nkx2.1 plays an important role in the development of mouse brain by regulating Shh signaling pathway. | Sansan Jia Li Zhang Kaili Zhang Lei Wang Ajab Khan Juan Zhang Yuqing Sun Yufei Wang Meiyan Song Yi Lyu Meining Li Xin Lu Bo Niu Zhizhen Liu Jun Xie | 2020 | Acta Biochimica et Biophysica Sinica2020，52，6： | 0 |
| 8 | 抑制Pin1通过NF-κB途经抑制大肠癌HCT116细胞的端粒酶(英文)显示文摘Objective: The aim of our study was to investigate the effect of Pin1 on the telomerase activity in human colorectal carcinoma HCT116 cells. Methods: Firstly, we transfected plasmid pGenesil-1-Pin1 (p-shRNA) using liposome (Lipofectamine 2000) into colorectal cancer HCT-116 cells to down-regulate the expression of Pin1. To detect the apoptotic rate of HCT116 cells was by cytometry (FCM). The expression of Pin1 and hTERT at RNA levels in human colorectal cancer HCT116 cells were determined by RT-PCR. To evaluate the activity of telomerase was by TRAP-silver staining. The subcellular localization and accumulative level of p-NF-κB/p65 protein at the nuclear was detected by Immunofluorescence and Western blotting. The DNA-binding activity of NF-κB/p65 was detected by electrophoretic mobility shift assay (EMSA). Results: Using liposome into colorectal cancer HCT-116 cells, and down-regulate the expression of Pin1 (0.392 ± 0.072-fold; P = 0.001), and the apoptotic rate was increased (11.40% ± 1.54%; P < 0.05). Compared with transfected p-CON cell group, in transfected p-shRNA cell group, the transcription of hTERT was lower (0.171 ± 0.060-fold; P = 0.001) by quantitative real-time RT-PCR, and the results of TRAP-silver staining analysis suggested that the telomerase activity was significantly declined (0.384 ± 0.015-fold; P < 0.05). Furthermore, it was demonstrated by Immunofluorescence that p-NF-κB/p65 had a nuclear localization, and the level of p-NF-κB/p65 protein at the nuclear was reduced with silencing the expression of Pin1 by Western blotting. Using EMSA, it was suggested that NF-κB/p65 was able to bind to hTERT promoter, and the direct interaction was declined with silencing the expression of Pin1. Conclusion: Taken together, silencing Pin1 may suppress activity of telomerase and the expression of hTERT by inhibiting NF-κB/p65 activity and reducing the combination of NF-κB/p65 and hTERT gene promoter. | Jianwen Sun Lijun Fan Meining Li Yuehong Zhang Niuliang Cheng | 2013 | The Chinese-German Journal of Clinical Oncology2013，12，4： | 0 |
| 9 | 降低Pin1可以抑制大肠癌细胞SW620的侵袭转移能力(英文)显示文摘Objective:The aim of our study was to investigate the effect of Pin1 on the expression of MMP-2 and MMP-9 in human colorectal carcinoma SW620 cells. Methods: We constructed a eukaryotic expression vector of RNA interfering (shRNA) for Pin1 gene (pGenesil-1-Pin1), and then observed its expression in SW620 cells by Western blotting. The cells motility were tested by wound healing assay and Boyden chamber assay. The protein levels and activity of MMP-2 and MMP-9 were tested by Western blotting and Gelatin zymography in SW620 cells after transfected with pGenesil-1-PIN1. Results: pGenesil-1-PIN1 was successfully constructed, which was confirmed by sequencing. Silencing the Pin1 by RNAi significantly decreased the cells motility from 96.4±3.9 per field (×10 objective) to 52.7±4.4 per field (P<0.05, Student's t-test) for SW620 cells transfected with pGenesil-1-PIN1 (SW620/p-shRNA) in Boyden chamber assay, and reduced the MMP-2 and MMP-9 expressions and activity in SW620 cells. The protein relative levels of MMP-2 were 0.32±0.04 for SW620/p-shRNA, and 0.76±0.03 for SW620/p-Con; MMP-9 were 0.41±0.09 for SW620/p-shRNA, and 0.94±0.07 for SW620/p-Con (p<0.05). Conclusion: Inhibited Pin1 expression may contribute to the suppression of the invasive and metastatic capacity of colon cancer cells in vitro. | Liyuan Qin Hua Hao Meining Li Dong Zhang Jianlin Zhang Niuliang Cheng | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，4： | 0 |
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