|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology显示文摘Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field. | Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang | 2021 | Engineering2021，7，9： | 4 |
| 2 | Solitary and Multiple Isolated Metastases of Clear Cell Renal Carcinoma to the Pancreas: An Indication for Pancreatic Surgery显示文摘 | Franz Sellner MD Natascha Tykalsky MD Maria Santis MD J?rg Pont MD M. Klimpfinger MD | 2006 | Annals of Surgical Oncology2006，，1： | 3 |
| 3 | Sphincter of oddi manometry in healthy volunteers显示文摘 | Dr. Moises Guelrud MD FACG Sonia Mendoza MD Guillermo Rossiter MD Maria I. Villegas MD | 1990 | Digestive Diseases and Sciences1990，，1： | 2 |
| 4 | Small bowel bacterial overgrowth in patients with alcoholic cirrhosis显示文摘 | Dr. F. Casafont Morencos MD G. Heras Casta?o MD L. Martín Ramos MD Maria J. López Arias MD F. Ledesma MD F. Pons Romero MD | 1996 | Digestive Diseases and Sciences1996，，3： | 2 |
| 5 | Histology of the healing tympanic membrane following perforation in rats 显示文摘 | Santa- Maria PL Redmond SL Atlas MD | 2010 | Laryngo- scope2010，120，10： | 1 |
| 6 | Solitary and Multiple Isolated Metastases of Clear Cell Renal Carcinoma to the Pancreas: An Indication for Pancreatic Surgery显示文摘 | Franz Sellner MD Natascha Tykalsky MD Maria Santis MD J?rg Pont MD M. Klimpfinger MD | 2006 | Annals of Surgical Oncology2006，，1： | 1 |
| 7 | Prevalence ofCampylobacter pylori and association with antral mucosal histology in subjects with and without upper gastrointestinal symptoms显示文摘 | Cynthia W. Pettross MD Maria D. Appleman PhD Hartley Cohen MD Jorge E. Valenzuela MD Parakrama Chandrasoma MD Loren A. Laine MD | 1988 | Digestive Diseases and Sciences1988，，6： | 1 |
| 8 | Lecithin Protects against plasma membrane disruptionby bile salts显示文摘 | Narain PK MD de Maria EJ | 1998 | J Surg Res1998，72，2： | 1 |
| 9 | Lack of effects of 2, 4 - dichlorophe- noxyacetic acid administration on markers of oxidative stress during early pregnancy in mice显示文摘 | Vinka MD Maria EH Maria FC | 2007 | Toxicology2007，237，13： | 1 |
| 10 | Lecithin Protects against plasma membrane disruptionby bile salts显示文摘 | Narain PK MD de Maria EJ | 1998 | J Surg Res1998，72，2： | 1 |
| 11 | Distribution of nitric oxide synthase and secretory role of exogenous nitric oxide in the isolated rat pancreas显示文摘 | Zsolt Ember MD Maria Dolores Yago Jaipaul Singh | 2001 | International Journal of Pancreatology2001，，2： | 1 |
| 12 | Evaluation of a liquid dressing for minor nonbleeding abrasions and classⅠandⅡskin tears in the emergency department显示文摘 | Adam John Singer MD Stuart Chale MD Maria Taylor | 2015 | The Journal of Emergency Medicine2015，48，2： | 1 |
| 13 | Thoracoscopic Sym- pathicotomy for Disabling Palmar Hyperhidrosis: A Prospective Randomized Comparison Between Two Levels 显示文摘 | Fritz J Baumgartner MD Maria Reyes | 2011 | Ann Thorac Surg2011，92，： | 1 |
| 14 | Vaginitis: diagnosis and treatment显示文摘 | Barrl L Hainer MD Maria V | 2011 | Am Fam Physician2011，83，7： | 1 |
| 15 | Breastfeeding the premature infant : experience of a baby-friendly hospital in brazil 显示文摘 | Maria BR Nascimento MD Hugo I | 2005 | Journal of Human Lactation2005，21，1： | 1 |
| 16 | Attenuating effects of melatonin on Pilocarpine induced seizures in rats显示文摘 | Leticia VC Maria MD Fonteles IS | 2002 | Comp Biochem Physiol C Toxicol Pharmacol2002，131，： | 1 |
| 17 | Evaluation of a new liquid occlusive dressing for excisional wounds显示文摘 | Adam J Singer MD Maria Nable MD | 2003 | Would Repair and Regeneration2003，11，3： | 1 |
| 18 | Identification of Risk Factors for Perioperative Mortality in Acute Mesenteric Ischemia显示文摘 | Maria Asuncion Acosta-Merida MD Joaquin Marchena-Gomez MD Marion Hemmersbach-Miller MD Cristina Roque-Castellano MD Juan Maria Hernandez-Romero MD | 2006 | World Journal of Surgery2006，，8： | 1 |
| 19 | Renal cell carcinoma in young and old patients:Is there a difference?显示文摘 | Houston Thompson MD Maria A | 2008 | J Urol2008，180，4： | 1 |
| 20 | Myocardial bridging in a patient with exertional chest pain 显示文摘 | Maria MD Antigoni MD Sophia MD | 2005 | Clinical Nuclear Medicine2005，30，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员