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| 1 | TRAIL-induced apoptosis of hepatocellular carcinoma cells is augmented by targeted therapies显示文摘AIM:To analyze the effect of chemotherapeutic drugs and specific kinase inhibitors,in combination with the death receptor ligand tumor necrosis factor-related apoptosis inducing ligand(TRAIL),on overcoming TRAIL resistance in hepatocellular carcinoma(HCC)and to study the efficacy of agonistic TRAIL antibodies,as well as the commitment of antiapoptotic BCL-2 proteins, in TRAIL-induced apoptosis. METHODS:Surface expression of TRAIL receptors (TRAIL-R1-4)and expression levels of the antiapoptotic BCL-2 proteins MCL-1 and BCL-xL were analyzed by flow cytometry and Western blotting,respectively. Knock-down of MCL-1 and BCL-xL was performed by transfecting specific small interfering RNAs.HCC cellswere treated with kinase inhibitors and chemotherapeutic drugs.Apoptosis induction and cell viability were analyzed via flow cytometry and 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. RESULTS:TRAIL-R1 and-R2 were profoundly expressed on the HCC cell lines Huh7 and Hep-G2. However,treatment of Huh7 and Hep-G2 with TRAIL and agonistic antibodies only induced minor apoptosis rates.Apoptosis resistance towards TRAIL could be considerably reduced by adding the chemotherapeutic drugs 5-fluorouracil and doxorubicin as well as the kinase inhibitors LY294002[inhibition of phosphoinositol- 3-kinase(PI3K)],AG1478(epidermal growth factor receptor kinase),PD98059(MEK1),rapamycin(mam- malian target of rapamycin)and the multi-kinase inhibitor Sorafenib.Furthermore,the antiapoptotic BCL-2 proteins MCL-1 and BCL-xL play a major role in TRAIL resistance:knock-down by RNA interference increased TRAIL-induced apoptosis of HCC cells.Additionally, knock-down of MCL-1 and BCL-xL led to a significant sensitization of HCC cells towards inhibition of both c-Jun N-terminal kinase and PI3K.CONCLUSION:Our data identify the blockage of survival kinases,combination with chemotherapeutic drugs and targeting of antiapoptotic BCL-2 proteins as promising ways to overcome TRAIL resistance in HCC. | Bruno Christian Koehler Toni Urbanik Binje Vick Regina Johanna Boger Steffen Heeger Peter R Galle Marcus Schuchmann Henning Schulze-Bergkamen | 2009 | World Journal of Gastroenterology2009，15，47： | 9 |
| 2 | The impact of patient and tumour baseline characteristics on the overall survival of patients with advanced hepatocellular carcinoma treated with sorafenib显示文摘 | Marcus A. W?rns Sandra Koch Ina M. Niederle Jens U. Marquardt Marc Nguyen-Tat Thomas Gamst?tter Marcus Schuchmann Henning Schulze-Bergkamen Peter R. Galle Arndt Weinmann | 2012 | Digestive and Liver Disease2012，，： | 1 |
| 3 | Identification of a specific sperm nuclei selenoenzyme necessary for protamine thiol cross-linking during sperm maturation显示文摘 | Henning P Marcus C Doris R | 2001 | FASEB2001，，10： | 1 |
| 4 | The role of apoptosis versus oncotic necrosis in liver injury:facts or faith?显示文摘 | Henning SB Marcus S Binje F | 2006 | J Hepatol2006，44，5： | 1 |
| 5 | Understanding health literacy from a traditional Chinese medicine perspective显示文摘Health literacy is critical to improving individual and public health.However,indigenous perceptions of health are largely absent from Western-derived measurements,contributing to disparities in health outcomes between indigenous and non-indigenous populations.China is the country with the world's largest population and only officially introduced the term'health literacy'in 2008.Current measures of health literacy in China are primarily based on Western-derived constructs,which have been shown to have poor comparability to the unique dual medical system in China.Given its significant importance to health management globally,understanding health perceptions from a traditional Chinese medicine perspective is essential.This review explores the concept and core elements of indigenous health literacy,evaluates the existing definitions and measurement tools as applied to the concept,and proposes a new model of traditional Chinese medicine health literacy. | Zhiyi Qian Grace Y.Wang Marcus Henning Yan Chen | 2023 | Journal of Integrative Medicine2023，21，3： | 0 |
| 6 | Live cold to grow old?Thermogenesis to fight cancer显示文摘A drop in ambient temperature induces systemic changes in glucose metabolism that enable thermogenesis.A recent article by Seki and colleagues explored the potential of cold exposure and brown fat activation to stop tumor growth. | Henning T.Langer Lewis C.Cantley Marcus D.Goncalves | 2022 | Cell Research2022，32，12： | 0 |
| 7 | Ventilation practices in burn patients—an international prospective observational cohort study显示文摘Background:It is unknown whether lung-protective ventilation is applied in burn patients and whether they benefit from it.This study aimed to determine ventilation practices in burn intensive care units(ICUs)and investigate the association between lung-protective ventilation and the number of ventilator-free days and alive at day 28(VFD-28).Methods:This is an international prospective observational cohort study including adult burn patients requiring mechanical ventilation.Low tidal volume(V_(T))was defined as V_(T)≤8 mL/kg predicted body weight(PBW).Levels of positive end-expiratory pressure(PEEP)and maximum airway pressures were collected.The association between V_(T) and VFD-28 was analyzed using a competing risk model.Ventilation settings were presented for all patients,focusing on the first day of ventilation.We also compared ventilation settings between patients with and without inhalation trauma.Results:A total of 160 patients from 28 ICUs in 16 countries were included.Low V_(T) was used in 74%of patients,median V_(T) size was 7.3[interquartile range(IQR)6.2–8.3]mL/kg PBW and did not differ between patients with and without inhalation trauma(p=0.58).Median VFD-28 was 17(IQR 0–26),without a difference between ventilation with low or high V_(T)(p=0.98).All patients were ventilated with PEEP levels≥5 cmH_(2)O;80%of patients had maximum airway pressures<30 cmH_(2)O.Conclusion:In this international cohort study we found that lung-protective ventilation is used in the majority of burn patients,irrespective of the presence of inhalation trauma.Use of low V_(T) was not associated with a reduction in VFD-28.Trial registration:Clinicaltrials.gov NCT02312869.Date of registration:9 December 2014. | Gerie J.Glas Janneke Horn Markus W.Hollmann Benedikt Preckel Kirsten Colpaert Manu Malbrain Ary Serpa Neto Karim Asehnoune Marcello Gamma de Abreu Ignacio Martin-Loeches Paolo Pelosi Folke Sjoerg Jan M.Binnekade Berry Cleffken Nicole P.Juffermans Paul Knape Bert G.Loef David P.Mackie Perenlei Enkhbaatar Nadia Depetris Anders Perner Eva Herrero Lucia Cachafeiro Marc Jeschke Jeffrey Lipman Matthieu Legrand Johannes Horter Athina Lavrentieva Alex Kazemi Anne Berit Guttormsen Frederik Huss Mark Kol Helen Wong Therese Starr Luc De Crop Wilson de Oliveira Filho Joa Manoel Silva Junior Cintia M.C.Grion Marjorie Burnett Frederik Mondrup Francois Ravat Mathieu Fontaine Renan Le Floch Mathieu Jeanne Morgane Bacus Maite Chaussard Marcus Lehnhardt Bassem Daniel Mikhail Jochen Gille Aidan Sharkey Nicole Trommel Auke C.Reidinga Nadine Vieleers Anna Tilsley Henning Onarheim Maria Teresa Bouza Alexander Agrifoglio Filip Fredén Tina Palmieri Lynda E.Painting Marcus J.Schultz LAMiNAR investigators | 2021 | Burns & Trauma2021，9，1： | 0 |
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