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| 1 | Relative pollen productivities of typical steppe species in northern China and their potential in past vegetation reconstruction显示文摘The Relative Pollen Productivities(RPPs)of common steppe species are estimated using Extended R-value(ERV)model based on pollen analysis and vegetation survey of 30 surface soil samples from typical steppe area of northern China.Artemisia,Chenopodiaceae,Poaceae,Cyperaceae,and Asteraceae are the dominant pollen types in pollen assemblages,reflecting the typical steppe communities well.The five dominant pollen types and six common types(Thalictrum,Iridaceae,Potentilla,Ephedra,Brassicaceae,and Ulmus)have strong wind transport abilities;the estimated Relevant Source Area of Pollen(RSAP)is ca.1000 m when the sediment basin radius is set at 0.5 m.Ulmus,Artemisia,Brassicaceae,Chenopodiaceae,and Thalictrum have relative high RPPs;Poaceae,Cyperaceae,Potentilla,and Ephedra pollen have moderate RPPs;Asteraceae and Iridaceae have low RPPs.The reliability test of RPPs revealed that most of the RPPs are reliable in past vegetation reconstruction.However,the RPPs of Asteraceae and Iridaceae are obviously underestimated,and those of Poaceae,Chenopodiaceae,and Ephedra are either slightly underestimated or slightly overestimated,suggesting that those RPPs should be considered with caution.These RPPs were applied to estimating plant abundances for two fossil pollen spectra(from the Lake Bayanchagan and Lake Haoluku)covering the Holocene in typical steppe area,using the'Regional Estimates of Vegetation Abundance from Large Sites'(REVEALS)model.The RPPs-based vegetation reconstruction revealed that meadow-steppe dominated by Poaceae,Cyperaceae,and Artemisia plants flourished in this area before 6500–5600 cal yr BP,and then was replaced by present typical steppe. | XU QingHai CAO XianYong TIAN Fang ZHANG ShengRui LI YueCong LI ManYue LI Jie LIU YaoLiang LIANG Jian | 2014 | Science China Earth Sciences2014，57，6： | 13 |
| 2 | Surface pollen assemblages of human-disturbed vegetation and their relationship with vegetation and climate in Northeast China显示文摘Pollen assemblages of 53 surface pollen samples from farmlands and wastelands in Northeast China were analyzed. Tree pollen percentages were usually higher than 30%, with Pinus (26.9%), Quercus (0.9%), Betula (0.9%) and Populus (0.7%) as the major types, and herb pollen percentages were usually higher than 50%, with weedy Poaceae (8.7%), Chenopodiaceae (7.1%), Artemisia (1.9%) and Compositae (3.5%) as the major taxa. Thus, the pollen assemblages were consistent with the regional vegetation compositions. However, there were differences in pollen assemblages among regions, especially among different geomorphological units. For example, in the mountains, there were more types of tree pollen and higher total percentages (average 42%) than in other areas, while cereal pollen percentages were lowest (11.2%). In the hills and high plains, herbs made up more than 60% of the pollens, with cereals (average 53.6%) the dominant type. In the low plains, pollen types were similar to those in the hills and high plains, but total pollen concentrations and the proportion of Concentricystes were much higher, while cereal pollen percentages were slightly lower (average 41.6%). Pollen assemblages in different land use types also differed. For example, in farmland, cereal pollen percentages were more than 40% and Chenopodiaceae was usually less than 2.5%, while in wasteland, weedy Poaceae was usually less than 10% and Chenopodiaceae was usually higher than 25%. Total pollen concentrations in farmland (average 3909 grains/g) were much lower than in wasteland (average 15074 grains/g). Redundancy analysis revealed that pollen assemblages were significantly negatively correlated with mean annual temperature (-0.73) and July mean temperature (-0.81) and significantly positively correlated with mean annual precipitation (0.48), indicating that pollen assemblages in the artificial or human-disturbed vegetation reflect regional climate well. Comparison of pollen assemblages in different areas of northern China showed that pollen concentrations reflect the intensities of human impact to some degree. For example, pollen concentrations decrease as human impacts increase in intensity. The cereal pollen proportions in farmland differed by area. In Northeast China, cereal pollen proportions were distinctly higher than in most other areas of northern China, suggesting differences in planting habits and climate. | LI ManYue LI YueCong XU QingHai PANG RuiMing DING Wei ZHANG ShengRui HE ZhiGuo | 2012 | Chinese Science Bulletin2012，57，5： | 10 |
| 3 | Spatial patterns of vegetation and climate in the North China Plain during the Last Glacial Maximum and Holocene climatic optimum显示文摘Reconstructing the spatial patterns of regional climate and vegetation during specific intervals in the past is important for assessing the possible responses of the ecological environment under future global warming scenarios. In this study, we reconstructed the history of regional vegetation and climate based on six radiocarbon-dated pollen records from the North China Plain. Combining the results with existing pollen records, we reconstruct the paleoenvironment of the North China Plain during the Last Glacial Maximum(LGM) and the Holocene Climatic Optimum(HCO). The results show that changes in the regional vegetation since the LGM were primarily determined by climatic conditions, the geomorphic landscape and by human activity.During the LGM, the climate was cold and dry;mixed broadleaf-coniferous forest and deciduous-evergreen broadleaf forest developed in the southern mountains, and cold-resistant coniferous forest and mixed broadleaf-coniferous forest were present in the northern mountains. The forest cover was relatively low, with mesophytic and hygrophilous meadow occupying the southern part of the plain, and temperate grassland and desert steppe were distributed in the north;Chenopodiaceae-dominated halophytes grew on the exposed continental shelf of the Bohai Sea and Yellow Sea. During the HCO, the climate was warm and wet;deciduous broadleaf forest and deciduous-evergreen broadleaf forest, with subtropical species, developed in the southern mountains, and deciduous broadleaf forest with thermophilic species was present in northern mountains. Although the degree of forest cover was greater than during the LGM, the vegetation of the plain area was still dominated by herbs, while halophytes had migrated inland due to sea level rise. In addition, the expansion of human activities, especially the intensification of cultivation,had a significant influence on the natural vegetation. Our results provide data and a scientific basis for paleoclimate modelling and regional carbon cycle assessment in north China, with implications for predicting changes in the ecological environment under future global warming scenarios. | Manyue LI Shengrui ZHANG Qinghai XU Jule XIAO Ruilin WEN | 2019 | Science China Earth Sciences2019，62，8： | 4 |
| 4 | Effects of Environmental Conditions and Aboveground Biomass on CO2 Budget in Phragmites australis Wetland of Jiaozhou Bay,China显示文摘Estuarial saline wetlands have been recognized as a vital role in CO_2 cycling.However,insufficient attention has been paid to estimating CO_2 fluxes from estuarial saline wetlands.In this study,the static chamber-gas chromatography(GC) method was used to quantify CO_2 budget of an estuarial saline reed(Phragmites australis) wetland in Jiaozhou Bay in Qingdao City of Shandong Province,China during the reed growing season(May to October) in 2014.The CO_2 budget study involved net ecosystem CO_2 exchange(NEE),ecosystem respiration(Reco) and gross primary production(GPP).Temporal variation in CO_2 budget and the impact of air/soil temperature,illumination intensity and aboveground biomass exerted on CO_2 budget were analyzed.Results indicated that the wetland was acting as a net sink of 1129.16 g/m^2 during the entire growing season.Moreover,the values of Reco and GPP were 1744.89 g/m^2 and 2874.05 g/m^2,respectively;the ratio of Reco and GPP was 0.61.Diurnal and monthly patterns of CO_2 budget varied significantly during the study period.Reco showed exponential relationships with air temperature and soil temperature at 5 cm,10 cm,20 cm depths,and soil temperature at 5 cm depth was the most crucial influence factor among them.Meanwhile,temperature sensitivity(Q10) of Reco was negatively correlated with soil temperature.Light and temperature exerted strong controls over NEE and GPP.Aboveground biomass over the whole growing season showed non-linear relationships with CO_2 budget,while those during the early and peak growing season showed significant linear relationships with CO_2 budget.This research provides valuable reference for CO_2 exchange in estuarial saline wetland ecosystem. | GAO Manyu KONG Fanlong XI Min LI Yue LI Jihua | 2017 | Chinese Geographical Science2017，27，4： | 2 |
| 5 | MAPK regulates cell cycle progression in pig oocytes and fertilized eggs显示文摘Oocytes collected from prepubertal gilt ovaries were matured in vitro (IVM), and fertilized in vitro (IVF) or electrically activated. Phosphorylation of mitogen-activated protein kinase (MAPK) was detected with SDS-PAGE and Western blotting, and translocation of ERK2 was observed with immunofluorescent cytochemistry. We found that the quantity of MAPK kept unchanged during oocyte maturation. There was no phosphorylated MAPK in porcine oocytes at the germinal vesicle (GV) stage; a little MAPK was phosphorylated at 20 h of IVM; a high level phosphory-lation was detected at 30 h, while MAPK phosphorylation decreased at 36 h; and then MAPK phosphorylation increased again to the peak level from 40 to 60 h. ERK2 translocated from the peripheral cytoplasm to inner cytoplasm and nuclear area during oocyte maturation. There was nearly no phosphorylated MAPK at 18 and 20 h of electrically activated oocytes, but phosphorylation increased at 22 h. There was no phosphorylated MAPK at 12 h of IVF, while | LI Manyu FAN Hengyu TONG Chao CHEN Dayuan XIA Guoliang SUN Qingyuan | 2002 | Chinese Science Bulletin2002，47，10： | 1 |
| 6 | Needleless Electrospinning:Reciprocation vs.Rotation显示文摘Needleless electrospinning is a versatile method to produce nanofibers.In particular,the rotary version of this technique has enjoyed widespread use because there is no need to clean the spinneret.The rotation speed is limited by the potential deviation of the jet due to the centrifugal force.Other limitations are due to the fast volatilization of the solvent from the opened spinning system.In order to overcome these drawbacks,here a novel reciprocating system based on a moving spinning-plate is proposed.The spinning process is implemented in a half-closed system with the spinning-plate immersed in the solution tank.When the immersed spinning-plate moves up from the solution tank,multiple jets are ejected from the droplets on the tips of the spinning-plate under the effect of an electric field force.The morphology of the obtained nanofibers has been analyzed by scanning electron microscopy.The results indicated that the obtained fibers are uniform in structures and small in diameters.Both issues of needle clogging and intense solvent evaporation can be mitigated using this alternate approach. | Xiaoxia Li Manyu Qian Dan Tian Jihuan He | 2021 | Fluid Dynamics & Materials Processing2021，17，6： | 0 |
| 7 | Design,application and verification of a novel system utilizing bacteria and microalgae to treat swine farm wastewater and produce value-added biomass显示文摘Swine farm wastewater is extremely harmful to the environment if not treated before it is discharged.In this study,a system was developed and optimized for testing the high levels of organic matter in swine farm wastewater utilizing a microalgae/bacteria co-culture combined with a novel closed-loop extraction and dilution process.Importantly,the system produces biomass that also could be harvested and used in value-added applications.The efficacy of biomass as a biofertilizer was demonstrated by using a model plant of Arabidopsis.In addition,the analysis of biomass indicates that it also has potential as a source for biofuel.After a 20-d cultivation period,a yield of biomass was achieved to 2.063 g/L of wastewater.The highest removal rates recorded in steady state conditions were:13.8 mg/L·d of Total Nitrogen(TN);11.5 mg/L·d of Ammonia Nitrogen(NH_(4)^(+)-N);24.8 mg/L·d of Chemical Oxygen Demand(COD);and 16.9 mg/L·d of Total Phosphorus(TP).After cultivation,the composition of the biomass was analyzed on a dry basis;the major components were protein(44.9%),lipids(24.6%),carbohydrates(19.9%),Chlorophyll-A(2.75%),Chlorophyll-B(1.66%),and carotenoids(0.57%).This biomass was diluted with water(5%by weight)and used as a biofertilizer to grow Arabidopsis.The results showed that the average root and stem lengths of Arabidopsis were 43.0%and 55.0%longer compared to those of the control group.Additionally,the number of leaves and the maximum leaf length increased by 30.2%and 39.7%;and the fresh and dry leaf weights increased by 44.0%and 33.7%,respectively.These results demonstrate the efficacy of this system for treating swine farm wastewater whilst simultaneously producing a value-added microalgae/bacteria biomass.This paper also demonstrated the use of biomass as a fertilizer for cultivating a value-added crop and,based on the compositional analyses,propose that the biomass could be used as a raw material for biofuel production due to its high lipid content of 24.6%.By constructing a microalgae/bacteria symbiosis system,Swine farm wastewater can be treated as resources utilizing producing value-added biomass with demonstrated efficacy as a biofertilizer. | Xin Zhen Yali Li Jingwen Qiang Manyu Tang Wei Hua Wanqing Wang Shuang Wu Phil Perkins Roger Ruan Yanling Cheng | 2023 | International Journal of Agricultural and Biological Engineering2023，16，4： | 0 |
| 8 | Reverse engineering a predictive signature characterized by proliferation,DNA damage,and immune escape from stage I lung adenocarcinoma recurrence显示文摘Identifying early-stage cancer patients at risk for progression is a major goal of biomarker research.This report describes a novel 19-gene signature(19-GCS)that predicts stage I lung adenocarcinoma(LAC)recurrence and responseto therapy and performs comparably in pancreatic adenocarcinoma(PAC),which shares LAC molecular traits.Kaplan-Meier,Cox regression,and cross-validation analyses were used to build the signature from training,test,and validation sets comprising 831 stage I LAC transcriptomes from multiple independent data sets.A statistical analysis was performed using the R Ianguage.Pathway and gene set enrichment were used to identify underlying mechanisms.19-GCS strongly predicts overall survival and recurrence-free survival in stage I LAC(P=0.002 and P<0.001,respectively)and in stage l-ll PAC(P<0.0001 and Pv0.0005,respectively).A multivariate cox regression analysis demonstrated the independence of 19-GCS from significant clinical factors.Pathway analyses revealed that 19-GCS high-risk LAC and PAC tumors are characterized by increased proliferation,enhanced sternness,DNA repair deficiency,and compromised MHC class I and II antigen presentation along with decreased immune infiltration.Importantly,high-risk LAC patients do not appear to benefit from adjuvant cisplatin while PAC patients derive additi onal ben efit from FOLFIRINOX compared with gemcitabi ne-based regimens.Whe n validated prospectively,this proof-of-c on cept biomarker may con tribute to tailoring treatment,recurrence reduction,and survival improvements in early-stage lung and pancreatic cancers. | Jiannan Yao Xinying Xue Dongfeng Qu C.Benedikt Westphalen Yang Ge Liyang Zhang Manyu Li Tianbo Gao Parthasarathy Chandrakesan Kenneth J.Vega Jun Peng Guangyu An Nathaniel Weygant | 2020 | Acta Biochimica et Biophysica Sinica2020，52，6： | 0 |
| 9 | Growth differentiation factor 11 promotes macrophage polarization towards M2 to attenuate myocardial infarction via inhibiting Notch1 signaling pathway显示文摘Background:Myocardial infarctions(MI)is a major threat to human health especially in people exposed to cold environment.The polarization of macrophages towards different functional phenotypes(M1 macrophages and M2 macrophages)is closely related to MI repairment.The growth differentiation factor 11(GDF11)has been reported to play a momentous role in inflammatory associated diseases.In this study,we examined the regulatory role of GDF11 in macrophage polarization and elucidated the underlying mechanisms in MI.Methods:In vivo,the mice model of MI was induced by permanent ligation of the left anterior descending coronary artery(LAD),and mice were randomly divided into the sham group,MI group,and MI+GDF11 group.The protective effect of GDF11 on myocardial infarction and its effect on macrophage polarization were verified by echocardiography,triphenyl tetrazolium chloride staining and immunofluorescence staining of heart tissue.In vitro,based on the RAW264.7 cell line,the effect of GDF11 in promoting macrophage polarization toward the M2 type by inhibiting the Notch1 Signaling pathway was validated by qRT-PCR,Western blot,and flow cytometry.Results:We found that GDF11 was significantly downregulated in the cardiac tissue of MI mice.And GDF11 supplementation can improve the cardiac function.Moreover,GDF11 could reduce the proportion of M1 macrophages and increase the accumulation of M2 macrophages in the heart tissue of MI mice.Furthermore,the cardioprotective effect of GDF11 on MI mice was weakened after macrophage clearance.At the cellular level,application of GDF11 could inhibit the expression of M1 macrophage(classically activated macrophage)markers iNOS,interleukin(IL)-1β,and IL-6 in a dose-dependent manner.In contrast,GDF11 significantly increased the level of M2 macrophage markers including IL-10,CD206,arginase 1(Arg1),and vascular endothelial growth factor(VEGF).Interestingly,GDF11 could promote M1 macrophages polarizing to M2 macrophages.At the molecular level,GDF11 significantly down-regulated the Notch1 signaling pathway,the activation of which has been demonstrated to promote M1 polarization in macrophages.Conclusions:GDF11 promoted macrophage polarization towards M2 to attenuate myocardial infarction via inhibiting Notch1 signaling pathway. | Manyu Gong Xuewen Yang Yaqi Wang Yanying Wang Dongping Liu Haodong Li Yunmeng Qu Xiyang Zhang Yanwei Zhang Han Sun Lei Jiao Ying Zhang | 2023 | Frigid Zone Medicine2023，3，1： | 0 |
| 10 | Long non-coding RNA-AK138945 regulates myocardial ischemia-reperfusion injury via the miR-1-GRP94 signaling pathway显示文摘Objective:Myocardial ischemia-reperfusion injury(MIRI)is one of the leading causes of death from cardiovascular disease in humans,especially in individuals exposed to cold environments.Long non-coding RNAs(lncRNAs)regulate MIRI through multiple mechanisms.This study explored the regulatory effect of lncRNA-AK138945 on myocardial ischemia-reperfusion injury and its mechanism.Methods:In vivo,8-to 12-weeks-old C57BL/6 male mice underwent ligation of the left anterior descending coronary artery for 50 minutes followed by reperfusion for 48 hours.In vitro,the primary cultured neonatal mouse ventricular cardiomyocytes(NMVCs)were treated with 100μmol/L hydrogen peroxide(H_(2)O_(2)).The knockdown of lncRNA-AK138945 was evaluated to detect cardiomyocyte apoptosis,and a glucose-regulated,endoplasmic reticulum stress-related protein 94(GRP94)inhibitor was used to detect myocardial injury.Results:We found that the expression level of lncRNA-AK138945 was reduced in MIRI mouse heart tissue and H2O2-treated cardiomyocytes.Moreover,the proportion of apoptosis in cardiomyocytes increased after lncRNA-AK138945 was silenced.The expression level of Bcl2 protein was decreased,and the expression level of Bad,Caspase 9 and Caspase 3 protein was increased.Our further study found that miR-1a-3p is a direct target of lncRNA-AK138945,after lncRNA-AK138945 was silenced in cardiomyocytes,the expression level of miR-1a-3p was increased while the expression level of its downstream protein GRP94 was decreased.Interestingly,treatment with a GRP94 inhibitor(PU-WS13)intensified H2O2-induced cardiomyocyte apoptosis.After overexpression of FOXO3,the expression levels of lncRNA-AK138945 and GRP94 were increased,while the expression levels of miR-1a-3p were decreased.Conclusion:LncRNA-AK138945 inhibits GRP94 expression by regulating miR-1a-3p,leading to cardiomyocyte apoptosis.The transcription factor Forkhead Box Protein O3(FOXO3)participates in cardiomyocyte apoptosis induced by endoplasmic reticulum stress through up-regulation of lncRNA-AK138945. | Yanying Wang Jian Huang Han Sun Jie Liu Yingchun Shao Manyu Gong Xuewen Yang Dongping Liu Zhuo Wang Haodong Li Yanwei Zhang Xiyang Zhang Zhiyuan Du Xiaoping Leng Lei Jiao Ying Zhang | 2024 | Frigid Zone Medicine2024，4，1： | 0 |
| 11 | Using nonparametric modeling approaches and remote sensing imagery to estimate ecological welfare forest biomass显示文摘The spatial distribution of forest biomass is closely related with carbon cycle, climate change, forest productivity, and biodiversity. Efficient quantification of biomass provides important information about forest quality and health. With the rising awareness of sustainable development, the ecological benefits of forest biomass attract more attention compared to traditional wood supply function. In this study, two nonparametric modeling approaches, random forest(RF) and support vector machine were adopted to estimate above ground biomass(AGB) using widely used Landsat imagery in the region,especially within the ecological forest of Fuyang District in Zhejiang Province, China. Correlation analysis was accomplished and model parameters were optimized during the modeling process. As a result, the best performance modeling method RF was implemented to produce an AGB estimation map. The predicted map of AGB in the study area showed obvious spatial variability and demonstrated that within the current ecological forest zone, as well as the protected areas, the average of AGB were higher than the ordinary forest. The quantification of AGB was proven to have a close relationship with the local forest policy and management pattern, which indicated that combining remote-sensing imagery and forest biophysical property would provide considerable guidance for making beneficial decisions. | Chaofan Wu Hongxiang Tao Manyu Zhai Yi Lin Ke Wang Jinsong Deng Aihua Shen Muye Gan Jun Li Hong Yang | 2018 | Journal of Forestry Research2018，29，1： | 0 |
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