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1Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro显示文摘Dear Editor,In December 2019,a novel pneumonia caused by a previously unknown pathogen emerged in Wuhan,a city of 11 million people in central China.The initial cases were linked to exposures in a seafood market in Wuhan.1 As of January 27,2020,the Chinese authorities reported 2835 confirmed cases in China's Mainland,including 81 deaths.Additionally,19 confirmed cases were identified in Hong Kong,Macao and Taiwan,and 39 imported cases were identified in Thailand,Japan,South Korea,United States,Vietnam,Singapore,Nepal,France,Australia and Canada.The pathogen was soon identified as a novel coronavirus(2019-nCoV),which is closely related to sever acute respiratory syndrome CoV(SARS-CoV).2 Currently,there is no specific treatment against the new virus.Therefore,identifying effective antiviral agents to combat the disease is urgently needed.Manli Wang Ruiyuan Cao Leike Zhang Xinglou Yang Jia Liu Mingyue Xu Zhengli Shi Zhihong Hu Wu Zhong Gengfu Xiao 2020Cell Research2020,30,3:563
2The empirical mode decomposition and the Hilbert spectrum for nonlinear and non-stationary time series analysis显示文摘Huang Norden E. Shen Zheng Long Steven R. Wu Manli C. Shih Hsing H. Zheng Quanan Yen Nai-Chyuan Tung Chi Chao Liu Henry H 1998Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences . 1998 (1971)1998,,1971:22
3The empirical mode decomposition and the Hilbert spectrum for nonlinear and non-stationary time series analysis显示文摘Huang Norden E. Shen Zheng Long Steven R. Wu Manli C. Shih Hsing H. Zheng Quanan Yen Nai-Chyuan Tung Chi Chao Liu Henry H 19981998 (1971)1998,,1971:18
4The empirical mode decomposition and the Hilbert spectrum for nonlinear and non-stationary time series analysis显示文摘Huang Norden E. Shen Zheng Long Steven R. Wu Manli C. Shih Hsing H. Zheng Quanan Yen Nai-Chyuan Tung Chi Chao Liu Henry H 1998Proceedings of the Royal Society A: Mathematical Physical and Engineering Sciences1998,,1971:15
5The empirical mode decomposition and the Hilbert spectrum for nonlinear and non-stationary time series analysis显示文摘Huang Norden E. Shen Zheng Long Steven R. Wu Manli C. Shih Hsing H. Zheng Quanan Yen Nai-Chyuan Tung Chi Chao Liu Henry H 1998Proceedings of the Royal Society A: Mathematical Physical and Engineering Sciences1998,,1971:10
6Fe_3O_4@C nanoparticles as high-performance Fenton-like catalyst for dye decoloration显示文摘Water pollution has become serious environmental problem nowadays. Advanced oxidation processes(AOP) have been widely applied in water treatment.However, traditional Fenton reaction based on Fe2﹢-H2O2 system has obvious drawbacks, which limit its applications In this study, magnetic Fe3O4core-C shell nanoparticles(Fe3O4@C NPs) were prepared for the decoloration of methylene blue(MB) via the co-precipitation followed by the hydrothermal dehydrogenation of glucose. Fe3O4@C NPs showed high catalytic activity of the decoloration of MB through the decomposition of H2O2 in Fenton-like reactions. Fe3O4@C NPs had much higher activity than bare Fe3O4 cores, suggesting the coating of carbon enhanced the catalytic activity. The performance of Fe3O4@C NPs was better at lower pH and higher temperature, but was significantly inhibited in the presence of radical scavenger tertiary butanol. Fe3O4@C NPs could be magnetic separated and regenerated, and maintained with very good catalytic activity. The implication for the applications of Fe3O4@C NP-catalyzed Fenton-like reactions in water treatment was discussed.Xiaoliang Zhang Manli He Jia-Hui Liu Rong Liao Lianqin Zhao Jingru Xie Ruijue Wang Sheng-Tao Yang Haifang Wang Yuanfang Liu 2014Chinese Science Bulletin2014,59,27:8
7Sequential fate-switches in stem-like cells drive the tumorigenic trajectory from human neural stem cells to malignant glioma显示文摘Glioblastoma(GBM)is an incurable and highly heterogeneous brain tumor,originating from human neural stem/progenitor cells(hNSCs/hNPCs)years ahead of diagnosis.Despite extensive efforts to characterize hNSCs and end-stage GBM at bulk and single-cell levels,the de novo gliomagenic path from hNSCs is largely unknown due to technical difficulties in early-stage sampling and preclinical modeling.Here,we established two highly penetrant hNSC-derived malignant glioma models,which resemble the histopathology and transcriptional heterogeneity of human GBM.Integrating time-series analyses of whole-exome sequencing,bulk and single-cell RNA-seq,we reconstructed gliomagenic trajectories,and identified a persistent NSC-like population at all stages of tumorigenesis.Through trajectory analyses and lineage tracing,we showed that tumor progression is primarily driven by multi-step transcriptional reprogramming and fate-switches in the NSC-like cells,which sequentially generate malignant heterogeneity and induce tumor phenotype transitions.We further uncovered stage-specific oncogenic cascades,and among the candidate genes we functionally validated C1QL1 as a new glioma-promoting factor.Importantly,the neurogenic-to-gliogenic switch in NSC-like cells marks an early stage characterized by a burst of oncogenic alterations,during which transient AP-1 inhibition is sufficient to inhibit gliomagenesis.Together,our results reveal previously undercharacterized molecular dynamics and fate choices driving de novo gliomagenesis from hNSCs,and provide a blueprint for potential early-stage treatment/diagnosis for GBM.Xiaofei Wang Ran Zhou Yanzhen Xiong Lingling Zhou Xiang Yan Manli Wang Fan Li Chuanxing Xie Yiming Zhang Zongyao Huang Chaoqiong Ding Kaidou Shi Weida Li Yu Liu Zhongwei Cao Zhen-Ning Zhang Shengtao Zhou Chong Chen Yan Zhang Lu Chen Yuan Wang 2021Cell Research2021,31,6:8
8Identification of Porphyra lines using computerized DNA fingerprinting显示文摘RAPD (Randomly Amplified Polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing four important groups (P. yezoensis, P. haitanensis, P. katadai var. Hemiphylla and P. digospermatangia). Eight stable and repeatable RAPD bands amplified with two primers, OPN-02 and OPJ-18, were selected for the construction of DNA fingerprinting. The RAPD results were scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of each band, respectively. Based on these results, a model DNA fingerprint and a computerized DNA fingerprint were constructed. In the constructed DNA fingerprint, each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other. Later, a software, named as PhGI, was designed based on this DNA fingerprinting. It can be used in practical Porphyra line identification.WangBin, Jia Jianhang, Shi Jinfeng, Chen Yihua, JinDemin, Xu Pu, Mei Junxue, Weng Manli 1. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 (E-mail: mlweng@genetics. ac. cn), China 2. Jiangsu Marine Fishery Institute, Nan 2001Acta Oceanologica Sinica2001,20,3:7
9Disparity in reimbursement for tuberculosis care among different health insurance schemes: evidence from three counties in central China显示文摘Background:Health inequity is an important issue all around the world.The Chinese basic medical security system comprises three major insurance schemes,namely the Urban Employee Basic Medical Insurance(UEBMI),the Urban Resident Basic Medical Insurance(URBMI),and the New Cooperative Medical Scheme(NCMS).Little research has been conducted to look into the disparity in payments among the health insurance schemes in China.In this study,we aimed to evaluate the disparity in reimbursements for tuberculosis(TB)care among the abovementioned health insurance schemes.Methods:This study uses a World Health Organization(WHO)framework to analyze the disparities and equity relating to the three dimensions of health insurance:population coverage,the range of services covered,and the extent to which costs are covered.Each of the health insurance scheme’s policies were categorized and analyzed.An analysis of the claims database of all hospitalizations reimbursed from 2010 to 2012 in three counties of Yichang city(YC),which included 1506 discharges,was conducted to identify the differences in reimbursement rates and out-of-pocket(OOP)expenses among the health insurance schemes.Results:Tuberculosis patients had various inpatient expenses depending on which scheme they were covered by(TB patients covered by the NCMS have less inpatient expenses than those who were covered by the URBMI,who have less inpatient expenses than those covered by the UEBMI).We found a significant horizontal inequity of healthcare utilization among the lower socioeconomic groups.In terms of financial inequity,TB patients who earned less paid more.The NCMS provides modest financial protection,based on income.Overall,TB patients from lower socioeconomic groups were the most vulnerable.Conclusion:There are large disparities in reimbursement for TB care among the three health insurance schemes and this,in turn,hampers TB control.Reducing the gap in health outcomes between the three health insurance schemes in China should be a focus of TB care and control.Achieving equity through integrated policies that avoid discrimination is likely to be effective.Yao Pan Shanquan Chen Manli Chen Pei Zhang Qian Long Li Xiang Henry Lucas 2016Infectious Diseases of Poverty2016,5,1:7
10Virus like particle-based vaccines against emerging infectious disease viruses显示文摘Emerging infectious diseases are major threats to human health.Most severe viral disease outbreaks occur in developing regions where health conditions are poor.With increased international travel and business,the possibility of eventually transmitting infectious viruses between different countries is increasing.The most effective approach in preventing viral diseases is vaccination.However,vaccines are not currently available for numerous viral diseases.Viruslike particles(VLPs) are engineered vaccine candidates that have been studied for decades.VLPs are constructed by viral protein expression in various expression systems that promote the selfassembly of proteins into structures resembling virus particles.VLPs have antigenicity similar to that of the native virus,but are non-infectious as they lack key viral genetic material.VLP vaccines have attracted considerable research interest because they offer several advantages over traditional vaccines.Studies have shown that VLP vaccines can stimulate both humoral and cellular immune responses,which may offer effective antiviral protection.Here we review recent developments with VLP-based vaccines for several highly virulent emerging or re-emerging infectious diseases.The infectious agents discussed include RNA viruses from different virus families,such as the Arenaviridae,Bunyaviridae,Caliciviridae,Coronaviridae,Filoviridae,Flaviviridae,Orthomyxoviridae,Paramyxoviridae,and Togaviridae families.Jinliang Liu Shiyu Dai Manli Wang Zhihong Hu Hualin Wang Fei Deng 2016Virologica Sinica2016,31,4:7
11Chromosome analysis of esophageal squamous cell carcinoma cell line KYSE 410-4 by repetitive multicolor fluorescence in situ hybridization显示文摘Chromosome aberrations are distinctive features of human malignant tumors. Analysis of chromosomal changes can illuminate the molecular mechanisms underlying the development and progression of cancer. To establish the technique of multicolor fluorescence in situ hybridization (M-FISH) for identifying chromosome aberrations in esophageal carcinoma cell line KYSE 410-4, four pools of 6-color whole-chromosome painting probes have been designed and hybridized on the same metaphase spread by four rounds of repetitive FISH. Repetitive 6-color M-FISH was successfully established and the cytogenetic abnormalities in KYSE 410-4 cells were characterized. Chromosome gains occurred at 2q, 3, 8, 17p, and X. An isochromosome 3q was visualized in the cell line, which might be one intermedi- ate mechanism leading to 3p losses and/or 3q gains. Furthermore, 16 structural arrangements were detected, including four derivative chromosomes. The rearrangement of the centromeric regions accounted for approximately 44% of all rearrangements. The results added a more complete and accurate information of the genetic alterations to the classical cytogenetic description of KYSE 410-4 and provided a detailed cytogenetic background data for appropriate use of the cell line. The established 6-color M-FISH was useful for analyzing chro- mosomes in the whole genome of human tumors.Yiling Yang Jiayou Chu Yupeng Wu Manli Luo Xin Xu Yaling Han Yan Cai Qimin Zhan Mingrong Wang 2008Journal of Genetics and Genomics2008,35,1:6
12Conservation of Jiaohe ancient earthen site in China显示文摘Earthen sites,which are mainly made of earth,are cultural heritages with historical,artistic and scientific values.Many extremely valuable earthen sites have been preserved in the arid areas in China.The earthen site of Jiaohe Ancient City is one of the earliest National Protected Important Cultural Heritage Sites.The Jiaohe Ancient City site exhibits all kinds of deteriorations,which can be found in the earthen sites in arid environments in China.Through a case study of the Jiaohe ancient earthen site,we present in this paper the comprehensive conservation technologies,including the mud bricklaying and reparation,wooden rod anchorage,crack grouting,surface potassium silicate(PS) penetration consolidation,and suspended steel beam ceiling,etc.Results of this case study showed that better conservation effects could be achieved by selecting proper PS penetration and crack grouting processes based on the deterioration characteristics of the earthen sites.The technology of mud bricklaying and reparation was also an effective method for preventing the earthen body from collapsing.Compared with traditional conservation technologies,the suspended steel beam ceiling technology could effectively reduce the negative impacts to the original state of the earthen site.As for unstable cliffs,a new method using composite anchor rod of bamboo and steel with massive loose earth was applied.Deformation monitoring and temporary supports were critical and indispensable measures for the safe of site conservation projects.Through years of monitoring and practical operation at the Jiaohe ancient earthen site,deterioration at the site has been effectively controlled.Zuixiong Li Xudong Wang Manli Sun Wenwu Chen Qinglin Guo Huyuan Zhang 2011Journal of Rock Mechanics and Geotechnical Engineering2011,3,3:6
13Genome analysis of Heliothis virescens ascovirus 3h isolated from China显示文摘No ascovirus isolated from China has been sequenced so far. Therefore, in this study, we aimed to sequence the genome of Heliothis virescens ascovirus 3h(Hv AV-3h) using the 454 pyrosequencing technology. The genome was found to be 190,519-bp long with a G+C content of 45.5%. We also found that it encodes 185 hypothetical open reading frames(ORFs) along with at least 50 amino acids, including 181 ORFs found in other ascoviruses and 4 unique ORFs. Gene-parity plots and phylogenetic analysis revealed a close relationship between Hv AV-3h and three other Hv AV-3a strains and a distant relationship with Spodoptera frugiperda ascovirus 1a(Sf AV-1a), Trichoplusia ni ascovirus 6a(Tn AV-6a), and Diadromus pulchellus ascovirus 4a(Dp AV-4a). Among the 185 potential genes encoded by the genome, 44 core genes were found in all the sequenced ascoviruses. In addition, 25 genes were found to be conserved in all ascoviruses except Dp AV-4a.In the Hv AV-3h genome, 24 baculovirus repeat ORFs(bros) were present, and the typical homologous repeat regions(hrs) were absent. This study supplies information important for understanding the conservation and functions of ascovirus genes as well as the variety of ascoviral genomes.Guo-Hua Huang Dian-Hai Hou Manli Wang Xiao-Wen Cheng Zhihong Hu 2017Virologica Sinica2017,32,2:6
14Containment of soft wall casing wrapped with Kevlar fabric显示文摘Aramid fabrics have been commonly used in the civil turbofan engine fan blade containment system for its excellent performance. To investigate the behavior and capability of soft wall containment casing, a series of fan blade released tests were conducted on the high-speed spin tester.The soft wall casing was fabricated by wrapping multiple layers of Kevlar49 plain woven fabric around a thin steel ring. Casings with different inner metal ring and outer fabric layers number were compared. The method of using the explicit dynamic software LS-DYNA to establish the finite element analysis model for the quantitative analysis of the containment process was developed and conducted. The simulation results are in good agreement with the test results. It is shown that the containment process of the soft wall casing can be divided into three impact stages. The casing with low-stiffness inner metal ring will get severe overall deformation and lose the structural integrity when it suffers the blade impact. Kevlar fabric layers will appear large bulge on outside surface and absorb the most impact dynamic energy of the high speed released fan blade. By summing up the results of the test and simulation, an empirical critical equation was derived to describe the relationship between the released blade dynamic energy and the Kevlar fabric thickness.Zekan HE Haijun XUAN Conger BAI Manli SONG Zhuoshen ZHU 2019Chinese Journal of Aeronautics2019,32,4:5
15Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres显示文摘The present study establishes a visualization method for the measurement of the distribution and localization of protein/peptide constituents within a single poly-lactide-co-glycolide(PLGA) microsphere using synchrotron radiation–based Fourier-transform infrared spectromicroscopy(SR-FTIR).The representative infrared wavenumbers specific for protein/peptide(Exenatide) and excipient(PLGA) were identified and chemical maps at the single microsphere level were generated by measuring and plotting the intensity of these specific bands.For quantitative analysis of the distribution within microspheres,Matlab software was used to transform the map file into a 3D matrix and the matrix values specific for the drug and excipient were extracted.Comparison of the normalized SR-FTIR maps of PLGA and Exenatide indicated that PLGA was uniformly distributed,while Exenatide was relatively non-uniformly distributed in the microspheres.In conclusion,SR-FTIR is a rapid,nondestructive and sensitive detection technology to provide the distribution of chemical constituents and functional groups in microparticles and microspheres.Manli Wang Xiaolong Lu Xianzhen Yin Yajun Tong Weiwei Peng Li Wu Haiyan Li Yan Yang Jingkai Gu Tiqiao Xiao Min Chen Jiwen Zhang 2015Acta Pharmaceutica Sinica B2015,5,3:5
16Monitoring of microbial community structure and succession in the biohydrogen production reactor by denaturing gradient gel electrophoresis(DGGE)显示文摘To study the structure of microbial communities in the biological hydrogen produc- tion reactor and determine the ecological function of hydrogen producing bacteria, anaerobic sludge was obtained from the continuous stirred tank reactor (CSTR) in different periods of time, and the diversity and dynamics of microbial communities were investigated by denaturing gra- dient gel electrophoresis (DGGE). The results of DGGE demonstrated that an obvious shift of microbial population happened from the beginning of star-up to the 28th day, and the ethanol type fermentation was established. After 28 days the structure of microbial community became stable, and the climax community was formed. Comparative analysis of 16S rDNA sequences from reamplifying and sequencing the prominent bands indicated that the dominant population belonged to low G+C Gram-positive bacteria (Clostridium sp. and Ethanologenbacterium sp.), β- proteobacteria (Acidovorax sp.), γ-proteobacteria (Kluyvera sp.), Bacteroides (uncultured bacte- rium SJA-168), and Spirochaetes (uncultured eubacterium E1-K13), respectively. The hydrogen production rate increased obviously with the increase of Ethanologenbacterium sp., Clostridium sp. and uncultured Spirochaetes after 21 days, meanwhile the succession of ethanol type fer- mentation was formed. Throughout the succession the microbial diversity increased however it decreased after 21 days. Some types of Clostridium sp. Acidovorax sp., Kluyvera sp., and Bac- teroides were dominant populations during all periods of time. These special populations were essential for the construction of climax community. Hydrogen production efficiency was de- pendent on both hydrogen producing bacteria and other populations. It implied that the co- metabolism of microbial community played a great role of biohydrogen production in the reactors.XING Defeng REN Nanqi GONG Manli LI Jianzheng LI Qiubo 2005Science China(Life Sciences)2005,48,2:5
17Inhibition of cyclooxygenase-2 activity in subchondral bone modifies a subtype of osteoarthritis显示文摘Osteoarthritis(OA) causes the destruction of joints. Its pathogenesis is still under investigation, and there is no effective diseasemodifying therapy. Here, we report that elevated cyclooxygenase-2(COX-2) expression in the osteocytes of subchondral bone causes both spontaneous OA and rheumatoid arthritis(RA). The knockout of COX-2 in osteocytes or treatment with a COX-2 inhibitor effectively rescues the structure of subchondral bone and attenuates cartilage degeneration in spontaneous OA(STR/Ort)mice and tumor necrosis factor-α transgenic RA mice. Thus, elevated COX-2 expression in subchondral bone induces both OAassociated and RA-associated joint cartilage degeneration. The inhibition of COX-2 expression can potentially modify joint destruction in patients with arthritis.Manli Tu Mi Yang Nanxi Yu Gehua Zhen Mei Wan Wenlong Liu Baochao Ji Hairong Ma Qiaoyue Guo Peijian Tong Li Cao Xianghang Luo Xu Cao 2019Bone Research2019,7,3:5
18Establishment of a Reverse Genetic System of Severe Fever with Thrombocytopenia Syndrome Virus Based on a C4 Strain显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV)is an emerging tick-borne bunyavirus that causes hemorrhagic fever-like disease(SFTS)in humans with a case fatality rate up to 30%.To date,the molecular biology involved in SFTSV infection remains obscure.There are seven major genotypes of SFTSV(C1-C4 and J1-J3)and previously a reverse genetic system was established on a C3 strain of SFTSV.Here,we reported successfully establishment of a reverse genetics system based on a SFTSV C4 strain.First,we obtained the 5’-and 3’-terminal untranslated region(UTR)sequences of the Large(L),Medium(M)and Small(S)segments of a laboratory-adapted SFTSV C4 strain through rapid amplification of cDNA ends analysis,and developed functional T7 polymerase-based L-,M-and S-segment minigenome assays.Then,fulllength cDNA clones were constructed and infectious SFTSV were recovered from co-transfected cells.Viral infectivity,growth kinetics,and viral protein expression profile of the rescued virus were compared with the laboratory-adapted virus.Focus formation assay showed that the size and morphology of the foci formed by the rescued SFTSV were indistinguishable with the laboratory-adapted virus.However,one-step growth curve and nucleoprotein expression analyses revealed the rescued virus replicated less efficiently than the laboratory-adapted virus.Sequence analysis indicated that the difference may be due to the mutations in the laboratory-adapted strain which are more prone to cell culture.The results help us to understand the molecular biology of SFTSV,and provide a useful tool for developing vaccines and antivirals against SFTS.Mingyue Xu Bo Wang Fei Deng Hualin Wang Manli Wang Zhihong Hu Jia Liu 2021Virologica Sinica2021,36,5:5
1950°S以南海域的感热潜热通量的模式计算显示文摘应用美国宇航局Goddard地球观测系统(GoddardEarthObservingSystem简称GEOS)四维资料同化系统(DataAssimilationSystem简称DAS),计算和分析了50°S以南海域夏季的感热、潜热通量.结果表明,在50°S以南海域潜热通量都为正值,感热通量在大部分海域亦为正值,但在有些海域可出现负值.感热和潜热通量随纬向呈高值区和低值区交替分布,通量低值区与海表温度低值槽区往往相符.李诗明 周明煜 吕乃平 苏立荣 ManLi CWu 吴志明 1997地球物理学报1997,40,4:4
20Bamboo charcoal enhances cellulase and urease activities during chicken manure composting:Roles of the bacterial community and metabolic functions显示文摘Microbial enzymes are crucial for material biotransformation during the composting process.In this study,we investigated the effects of adding bamboo charcoal(BC)(i.e.,at 5%,10%,and 20%corresponding to BC5,BC10,and BC20,respectively)on the enzyme activity levels during chicken manure composting.The results showed that BC10 could increase the cellulose and urease activities by 56%and 96%,respectively.The bacterial community structure in BC10 differed from those in the other treatments,and Luteivirga,Lactobacillus,Paenalcaligenes,Ulvibacter,Bacillus,Facklamia,Pelagibacterium,Sporosarcina,Cellvibrio,and Corynebacterium had the most important roles in composting.Compared with other treatments,BC10 significantly enhanced the average rates of degradation of carbohydrates(Dxylose(40%)andα-D-lactose(44%))and amino acids(L-arginine(16%),L-asparagine(14%),and L-threonine(52%)).We also explored the associations among the bacterial community and their metabolic functions with the changes in the activities of enzymes.Network analysis demonstrated that BC10 altered the co-occurrence patterns of the bacterial communities,where Ulvibacter and class Bacilli were the keystone bacterial taxa with high capacities for degrading carbon source,and they were related to increases in the activities of cellulase and urease,respectively.The results obtained in this study may help to further enhance the efficiency of composting.Yanan Yin Chao Yang Jingrui Tang Jie Gu Haichao Li Manli Duan Xiaochang Wang Rong Chen 2021Journal of Environmental Sciences2021,33,10:4
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