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| 1 | Mucosal bacterial microflora and mucus layer thickness in adolescents with inflammatory bowel disease显示文摘AIM:To assess the mucosa-associated bacterial microflora and mucus layer in adolescents with inflammatory bowel disease(IBD) .METHODS:Sixty-one adolescents(mean age 15 years,SD ± 4.13) were included in the study.Intestinal biopsies from inflamed and non-inflamed mucosa of IBD patients and from controls with functional abdominal pain were cultured under aerobic and anaerobic conditions.The number of microbes belonging to the same group was calculated per weight of collected tissue.The mucus thickness in frozen samples was measured under a fluorescent microscope.RESULTS:The ratios of different bacterial groups in inflamed and non-inflamed mucosa of IBD patients and controls were specific for particular diseases.Streptococcus spp.were predominant in the inflamed mucosa of Crohn's disease(CD) patients(80% of all bacteria) ,and Lactobacillus spp.were predominant in ulcerative colitis patients(90%) .The differences were statistically significant(P = 0.01-0.001) .Lower number of bifidobacteria was observed in the whole IBD group.A relation was also found between clinical and endoscopic severity and decreased numbers of Lactobacillus and,to a lesser extent,of Streptococcus in biopsies from CD patients.The mucus layer in the inflamed sites was significantly thinner as compared to controls(P = 0.0033) and to non-inflamed areas in IBD patients(P = 0.031) .CONCLUSION:The significantly thinner mucosa of IBD patients showed a predominance of some aerobes specific for particular diseases,their numbers decreased in relation to higher clinical and endoscopic activity of the disease. | Krzysztof Fyderek Magdalena Strus Kinga Kowalska-Duplaga Tomasz Gosiewski Andrzej Wedrychowicz Urszula Jedynak-Wasowicz Malgorzata Sladek Stanislaw Pieczarkowski Pawel Adamski Piotr Kochan Piotr B Heczko | 2009 | World Journal of Gastroenterology2009，15，42： | 14 |
| 2 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 3 | Dietary and socio-economic factors in relation to Helicobacter pylori re-infection显示文摘AIM:To examine if dietary and socio-economic factors contribute to Helicobacter pylori(H pylori)re-infection. METHODS:The population of patients consisted of subjects in whom H pylori infection had been successfully treated in the past.Patients were divided into two groups:Ⅰ-examined group(111 persons with H pylori re-infection)andⅡ-control group(175 persons who had not been re-infected).The respondents were interviewed retrospectively on their dietary habits and socio-economic factors. RESULTS:A statistically significant lower frequency of fermented dairy products(P<0.0001),vegetables (P=0.02),and fruit(P=0.008)consumption was noted among patients with H pylori re-infection as compared to those who had not been re-infected. CONCLUSION:High dietary intake of probiotic bacteria,mainly Lactobacillus,and antioxidants,mainly vitamin C(contained in fruit and vegetables),might decrease the risk of H pylori re-infection. | Mirosaw Jarosz Ewa Rychlik Magdalena Siuba Wioleta Respondek Magorzata Ryzko-Skiba Iwona Sajór Sylwia Gugaa Tomasz Bazejczyk Janusz Ciok | 2009 | World Journal of Gastroenterology2009，15，9： | 6 |
| 4 | Modeling the tryptic hydrolysis of pea proteins usingan artificial neural network显示文摘 | ADAM B MAGDALENA K RYSZARD A | | 0，，： | 1 |
| 5 | Pyrite oxidation by Acidithiobacillus ferrooxidans at various concentrations of dissolved oxygen显示文摘 | Magdalena G Herbert Jr Roger B Frogner Kockum Paul C | 2006 | Chemical Geology2006，225，12： | 1 |
| 6 | Scheduling flow shops with blocking using a discrete self-organising migrating algorithm 显示文摘 | DONALD D MAGDALENA B D | 2013 | International Journal of Production Research2013，51，8： | 1 |
| 7 | The Dexela 2923 CMOS X-ray Detector: A flat panel detector based on CMOS active pixel sensors for medical imaging applications显示文摘 | Anastasios C K Magdalena B S Robert D S | 2012 | Nuclear Instruments and Methods in Physics and Research A2012，68，912： | 1 |
| 8 | Hydrothermal synthesis of acicular lead zirconate titanate显示文摘 | Seung B C Magdalena O Richard E R | 2001 | J Crystal Growth2001，226，： | 1 |
| 9 | Cross-coupling of vinyldisilacyclobutane with olefins catalyzed by 显示文摘 | Magdalena J Olga S Natalia B | 2005 | J Organomet Chem2005，690，10： | 1 |
| 10 | Liquid chromatography-multiple tandem mass spectrometry for the determination of ten azaspiracids,including hydroxyl analogues in shellfish显示文摘 | Lehane M Sáez M J F Magdalena A B | 2004 | J ChromatograA2004，1024，： | 1 |
| 11 | Interactions of free copper (Ⅱ) ions alone or incomplex with iron ( Ⅲ ) ions with erythrocytes of ma- rine fish Dicentrarchus labrax 显示文摘 | MAGDALENA L KATARAYNA M ANETA B | 2009 | Cell Biol Int2009，33，： | 1 |
| 12 | Dynamic High Pressure Calibration of the Fiber - Optic Sensor Based on Bi- refringent Side - Hole Fibers 显示文摘 | Magdalena S N Wojtek J B Urbanczyk W | 2005 | IEEE Sensors Journal2005，5，5： | 1 |
| 13 | Interleukin -1β,interleukin-6,and tumor necrosis factor-a in endometriotic tissue and in endometrium显示文摘 | Agneta B Christine B Magdalena C | 2001 | Fertil Steril2001，75，3： | 1 |
| 14 | A Novel Active Packaging for Extending the Shelf-life of Fresh Mushrooms (Agaricus Bisporus) 显示文摘 | MAGDALENA W KARIM B CRISTINA N | 2015 | Food Control2015，54，： | 1 |
| 15 | The Plackett-Burman design in optimization of media components for biomass production of Lactobacillus rhamnosus OXY 显示文摘 | WASKO MONIKA K W PODLESNY M MAGDALENA P B TARGONSKI Z AGNIESZKA K K | 2010 | Acta Biologica Hungarica2010，61，3： | 1 |
| 16 | Bradykinin B2 receptor endocytosis, recycling, and down-regulation assessed using green fluorescent protein conjugates显示文摘 | Dimcho R B Steeve H Magdalena B | 2001 | J Pharm Exper Therap2001，297，1： | 1 |
| 17 | Comparison Of Radical-Scavenging Activities for Selected Phenolic Acids显示文摘 | Magdalena Karama Agnieszka Kosiska Ronald B Pegg | 2005 | Polish Journal of Food and Nutrition Sciences2005，55，14： | 1 |
| 18 | Liquid chromatography with electrospray ion trap mass spectrometry for the determination of five azaspiracids in shellfish显示文摘 | Lehane M Magdalena A B Moroney C | 2002 | J Chromatogr2002，950，： | 1 |
| 19 | Comparison of solid-phase extraction methods for the determination of azaspiracids in shellfish by liquid chromatography-electrospray mass spectrometry显示文摘 | Moroney C Lehane M Magdalena A B | 2002 | J Chromatogr2002，963，： | 1 |
| 20 | Determination of free radicals and flavan-3-ols content in fermented and unfermented teas and properties of their infusions显示文摘 | Robert Socha Ma?gorzata B?czkowicz Teresa Fortuna Agnieszka Kura Maria ?abanowska Magdalena Kurdziel | 2013 | European Food Research and Technology2013，，2： | 1 |
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