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| 1 | Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms显示文摘The infusion of coronavirus disease 2019(COVID-19)patients with mesenchymal stem cells(MSCs)potentially improves clinical symptoms,but the underlying mechanism remains unclear.We conducted a randomized,single-blind,placebo-controlled(29 patients/group)phase II clinical trial to validate previous findings and explore the potential mechanisms.Patients treated with umbilical cord-derived MSCs exhibited a shorter hospital stay(P=0.0198)and less time required for symptoms remission(P=0.0194)than those who received placebo.Based on chest images,both severe and critical patients treated with MSCs showed improvement by day 7(P=0.0099)and day 21(P=0.0084).MSC-treated patients had fewer adverse events.MSC infusion reduced the levels of C-reactive protein,proinflammatory cytokines,and neutrophil extracellular traps(NETs)and promoted the maintenance of SARS-CoV-2-specific antibodies.To explore how MSCs modulate the immune system,we employed single-cell RNA sequencing analysis on peripheral blood.Our analysis identified a novel subpopulation of VNN2+hematopoietic stem/progenitorlike(HSPC-like)cells expressing CSF3R and PTPRE that were mobilized following MSC infusion.Genes encoding chemotaxis factors—CX3CR1 and L-selectin—were upregulated in various immune cells.MSC treatment also regulated B cell subsets and increased the expression of costimulatory CD28 in T cells in vivo and in vitro.In addition,an in vivo mouse study confirmed that MSCs suppressed NET release and reduced venous thrombosis by upregulating kindlin-3 signaling.Together,our results underscore the role of MSCs in improving COVID-19 patient outcomes via maintenance of immune homeostasis. | Rongjia Zhu Tingdong Yan Yingmei Feng Yan Liu Hongcui Cao Gongxin Peng Yanlei Yang Zhen Xu Jingqi Liu Wei Hou Xiaoyue Wang Zhe Li Luchan Deng Shihua Wang Jing Li Qin Han Hongling Li Guangliang Shan Yinghao Cao Xingyan An Jianshe Yan Zhonghui Zhang Huafei Li Xuebin Qu Jiaqi Zhu Shumin Zhou Jiao Wang Fengchun Zhang Jinming Gao Ronghua Jin Dayong Xu Yan-Qing Ma Tao Huang Shuang Peng Zhi Zheng Ilia Stambler Eric Gilson Lee Wei Lim Alexey Moskalev Antonio Cano Sasanka Chakrabarti Brun Ulfhake Huanxing Su Haoying Xu Sihuan Xu Feng Wei Holly MBrown-Borg Kyung-Jin Min Georgina Ellison-Hughes Calogero Caruso Kunlin Jin Robert Chunhua Zhao | 2021 | Cell Research2021，31，12： | 5 |
| 2 | Experimental investigation on dynamic mechanical characteristics and microstructure of steam-cured concrete显示文摘Steam-cured concrete is widely used to manufacture prefabricated units of high-speed railway foundation structure such as girder and track slab.The dynamic mechanical property of steam-cured concrete is one of the key properties affecting service performance of high-speed railway foundation structure.In the present paper,serial macro/micro-experiments were carried out to investigate the dynamic elastic modulus,shear modulus,damping ratio,and microstructure of steam-cured concrete.The relationships between compositions,curing regime,microstructure,and dynamic properties of steam-cured concrete as well as the corresponding mechanisms were discussed.The results indicate that steam-cured concrete in early age has a larger dynamic elastic modulus and shear modulus as well as a smaller damping ratio compared with standard-cured concrete.On the contrary,at a later age a slightly smaller dynamic elastic modulus and a larger damping ratio of steam-cured concrete are observed.Addition of mineral admixture results in a bit lower dynamic elastic modulus and damping ratio of concrete than that of the control specimen without mineral admixtures.The achievements can provide some fundamental suggestions for materials parameters selection during structural design of steam-cured concrete precast element. | LONG GuangCheng WANG Meng XIE YouJun MA KunLin | 2014 | Science China(Technological Sciences)2014，57，10： | 4 |
| 3 | Deterioration of dynamic mechanical property of concrete with mineral admixtures under fatigue loading显示文摘The dynamic mechanical property of concrete is one of the key parameters,which greatly influences durability of infrastructures subjected to continuous heavy loading,such as girder and track slab of high-speed railway foundation structure.This paper reports serials of experiments designed to investigate the deterioration of dynamic mechanical properties of different concretes under fatigue loading condition.Four parameters including relative dynamic elastic modulus(RDEM),relative dynamic shear modulus(RDSM),relative compressive strength(RCS)and water absorption(WA)of concrete were evaluated to assess the dynamic properties and microstructures of concretes.Results show that the fatigue stress levels and fatigue cycle durations significantly influence the dynamic mechanical properties of concrete including dynamic elastic modulus and dynamic shear modulus.Addition of proper mineral admixture can improve the dynamic mechanical characteristics of concrete and increase its resistance against the fatigue loading effect.Keeping the amount of mineral admixture in concrete constant,its dynamic mechanical property with fly ash is lower than that with fly ash and silica fume.The water absorption in concrete,which is an indirect parameter reflecting capillary porosity,increases evidently after bearing fatigue-loading.There is a close correlation between the deterioration of dynamic mechanical property and the increasing of water absorption of concrete.This indicates that the damage of microstructure of concrete subjected to fatigue loading is the indispensable reason for the decay of its dynamic mechanical performance. | MA KunLin XIE YouJun LONG GuangCheng TAN WenYong | 2014 | Science China(Technological Sciences)2014，57，10： | 2 |
| 4 | Self-compacting concrete reinforced by waste tyre rubber particle and emulsified asphalt显示文摘 | Long Guangcheng Ma Kunlin Zhe Li | 2012 | Sustainable Construction Materials2012，，144： | 1 |
| 5 | Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’显示文摘Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis(SE) is an efficient method for mass production of Hippeastrum plantlets. Previous studies have been devoted to the in vitro propagation of Hippeastrum, but the SE and its regulatory networks are rarely reported. In this study, we established a direct SE method of Hippeastrum Bangkok Rose' using leaf bases as explants. MS supplemented with 1.00 mg·L^(-1)NAA +1.00 mg·L^(-1)KT + 0.25 mg·L^(-1)TDZ was the optimal medium for SE. Histological observations showed that the bipolar somatic embryo originated from the epidermal cell layer and underwent initiation,globular, scutellar and coleoptile stages. During SE, endogenous hormones of IAA, CTK, ABA, and SA were highly accumulated. Transcriptomic analysis revealed the genes encoding auxin biosynthesis/metabolic enzymes and efflux carriers were induced, while the auxin receptor of TIR1 and ARF transcriptional repressor of Aux/IAA were down-regulated and up-regulated, respectively, leading to suppression of auxin signaling. In contrast, cytokine signaling was promoted at the early stage of SE, as biosynthesis, transport, and signaling components were up-regulated.Various stress-related genes were up-regulated at the early or late stages of SE. Chromatin remodeling could also be dynamically regulated via distinct expression enzymes that control histone methylation and acetylation during SE. Moreover, key SE regulators, including WOXs and SERKs were highly expressed along with SE. Overall, the present study provides insights into the SE regulatory mechanisms of the Hippeastrum. | Jingjue Zeng Yi Deng Shahid Iqbal Jiarui Zhang Kunlin Wu Guohua Ma Lin Li Guangyi Dai Rufang Deng Lin Fang Songjun Zeng | 2024 | Horticultural Plant Journal2024，10，2： | 0 |
| 6 | Effects of Limestone Powder on Rheological Properties of Cement - Fly Ash Mortar显示文摘 | XIE Youjun CHEN Xiaobo MA Kunlin FENG Jin ZHAN Fang | 2015 | International English Education Research2015，，12： | 0 |
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