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| 1 | Identification and characterization of putative CIPK genes in maize显示文摘当在细胞内部的发信号的第二个送信人由发展、环境的暗示得到了,钙(Ca ) 起一个关键作用。Calcineurin 象 B 一样蛋白质(CBL ) 和他们的目标蛋白质,交往 CBL 蛋白质 kinases (CIPK ) 在植物响应压力作为一个关键调停 Ca2+ 的发信号网络出现了。生物信息的分析被用来识别 43 通常认为的 ZmCIPK (Zea mays CIPK ) 在玉米的染色体的基因生来的线 B73。基于基因结构,这些 ZmCIPKs 被划分成 intron 富有、 intron 差的组。种系发生的分析显示 ZmCIPK 家庭与 30 个成员的米饭 CIPK 家庭一起有一种高进化的关系。Microarray 数据和 RT-PCR 试金显示出基因 transcriptionally 回答了到不能生活的压力,和基因是的那 24, 31, 20 和 19 ZmCIPK 的那 ZmCIPK 由盐,干旱,热和冷压力起来调整分别地。在冷应力下面有在寒冷容忍的生来的线 B73 和寒冷敏感的生来的线 Mo17 之间的 ZmCIPKs 的不同表示模式。我们的调查结果将在玉米帮助 CIPK 的生物功能的进一步分子的解剖,并且提供新卓见进在植物表明网络的 CBLCIPK。 | Xifeng Chen Zhimin Gu Dedong Xin Liang Hao Chengjie Liu Ji Huang Bojun Ma Hongsheng Zhang | 2011 | Journal of Genetics and Genomics2011，38，2： | 40 |
| 2 | Characterization and cloning of a brittle culm mutant (bc88) in rice (Oryza sativa L.)显示文摘This study characterizes a brittle culm (bc88) mutant of rice (Oryza sativa L.) obtained by ethylene methylsulfonate (EMS)-induced mutagenesis of Wuyunjing 7. The bc88 mutant exhibits a diversity of pleiotropic phenotypes, including brittle culm at the whole-plant growth stages, withered leaf tips at the seedling stage, and 18-d delay in heading date at the mature stage. Genetic analysis indicates that the bc88 mutant is controlled by a single recessive nuclear gene. The mutated bc88 gene isolated by map-based cloning contains only one point mutation in the 5th exon relative to its wild-type BC88 (LOC_Os09g25490 and Os09g0422500), leading to an amino acid change from P to L in bc88 plants. Alignment of the putative protein sequence with its homologs indicates that the mutation is located in the conserved region of the sequence. Detection of the transcription level of BC88 in rice plants shows that the expression level of BC88 is higher in spikes and culms than in leaves, roots, and leaf sheaths. These contribute to understanding of the molecular mechanism of cellulose synthesis. The target gene BC88 can be a useful tool in molecular marker-assisted selection for rice culm trait breeding. | RAO YuChun YANG YaoLong XIN DeDong LI XiaoJing ZHAI KaiEn MA BoJun PAN JianWei QIAN Qian ZENG DaLi | 2013 | Chinese Science Bulletin2013，58，24： | 11 |
| 3 | Xa7, a new executor R gene that confers durable and broad-spectrum resistance to bacterial blight disease in rice显示文摘Bacterial blight(BB)is a globally devastating rice disease caused by Xanthomonas oryzae pv.oryzae(Xoo).The use of disease resistance(R)genes in rice breeding is an effective and economical strategy for the control of this disease.Nevertheless,a majority of R genes lack durable resistance for long-term use under global warming conditions.Here,we report the isolation of a novel executor R gene,Xa7,that confers extremely durable,broad-spectrum,and heat-tolerant resistance to Xoo.The expression of Xa7 was induced by incompatible Xoo strains that secreted the transcription activator-like effector(TALE)AvrXa7 or PthXo3,which recognized effector binding elements(EBEs)in the Xa7 promoter.Furthermore,Xa7 induction was faster and stronger under high temperatures.Overexpression of Xa7 or co-transformation of Xa7 with avrXa7 triggered a hypersensitive response in plants.Constitutive expression of Xa7 activated a defense response in the absence of Xoo but inhibited the growth of transgenic rice plants.In addition,analysis of over 3000 rice varieties showed that the Xa7 locuswas found primarily in the indica and aus subgroups.A variation consisting of an 11-bp insertion and a base substitution(G to T)was found in EBEAvrXa7 in the tested varieties,resulting in a loss of Xa7 BB resistance.Through a decade of effort,we have identified an important BB resistance gene and characterized its distinctive interaction with Xoo strains;these findings will greatly facilitate research on the molecular mechanism of Xa7-mediated resistance and promote the use of this valuable gene in breeding. | Xifeng Chen Pengcheng Liu Le Mei Xiaoling He Long Chen Hui Liu Shurong Shen Zhandong Ji Xixi Zheng Yuchen Zhang Zhenyu Gao Dali Zeng Qian Qian Bojun Ma | 2021 | Plant Communications2021，2，3： | 9 |
| 4 | SYSTEMATIC METHOD TO GENERATE NEW IDEAS IN FUZZY FRONT END USING TRIZ显示文摘The obstacle for idea generation in fuzzy fi'ont end (FFE) is difficult to apply knowledge in different fields for designers.Theory of inventive problem solving TRIZ and computer-aided innovation systems (CAIs) which are TRIZ-base software systems with a knowledge base provide a framework for knowledge application in different fields.The major methods in TRIZ are selected, which have four types.The problems to be solved for each method are summarized and mapping from the problems to the methods is given.Systematic method with eight paths to integrate the methods and problems is formed.A case study shows the idea generation in FFE using the integrated method step by step. | TAN Runhua MA Lihui YANG Bojun SUN Jianguang | 2008 | Chinese Journal of Mechanical Engineering2008，21，2： | 6 |
| 5 | Fine mapping of the rice bacterial blight resistance gene Xa-4 and its co-segregation marker显示文摘An F2 population developed from the Xa-4 near isogenic lines, IR24 and IRBB4, was used for fine mapping of the rice bacterial blight resistance gene, Xa-4. Some restriction fragment length polymorphism (RFLP) markers on the high-density map constructed by Harushima et al. and the amplified DMA fragments homologous to the conserved domains of plant disease resistance (R) genes were used to construct the genetic linkage map around the gene Xa-4 by scoring susceptible individuals in the population. Xa-4 was mapped between the RFLP marker G181 and the polymerase chain reaction (PCR) marker M55. The R gene homologous fragment marker RS13 was found co-segregating with Xa-4 by analyzing all the plants in the population. This result opened an approach to map-based cloning of this gene, and marker RS13 can be applied to molecular marker-assisted selection of Xa-4 in rice breeding programs. | WANG Wenming ZHOU Yongli JIANG Guanhuai MA Bojun CHEN Xuewei ZHANG Qi ZHU Lihuang ZHAI Wenxue | 2000 | Chinese Science Bulletin2000，45，19： | 4 |
| 6 | MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice显示文摘Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1(mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms and delayed heading date.By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOC_Os09g02650(BC12) in mtd1 mutant had a single nucleotide substitution(G to A), which generated a premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all the phenotypes of mtd1 mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtd1 mutant.Quantitative reverse transcription-PCR analysis showed that MTD1/BC12 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remarkably, a-amylase activity analysis and gibberellic acid(GA)treatment showed that the dwarf phenotype of mtd1 mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice. | Haiping Yu Deyong Ren Yangzhou Zhu Jiangmin Xu Yuexing Wang Ruifang Liu Yunxia Fang Zhenyuan Shi Jiangjie Pan Mei Lu Bojun Ma Jiang Hu Yuchun Rao | 2016 | Science Bulletin2016，61，23： | 3 |
| 7 | Expression analysis of the calcineurin B-like gene family in rice ( Oryza sativa L.) under environmental stresses显示文摘 | Zhimin Gu Bojun Ma Yan Jiang Zhiwei Chen Xuan Su Hongsheng Zhang | 2008 | Gene2008，，1： | 3 |
| 8 | SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice显示文摘 | Xifeng Chen Liang Hao Jianwei Pan Xixi Zheng Guanghuai Jiang Yang Jin Zhimin Gu Qian Qian Wenxue Zhai Bojun Ma | 2012 | Molecular Breeding2012，，2： | 2 |
| 9 | A motion planning based adaptive control method for an underactuated crane system 显示文摘 | FANG Yongchun MA Bojun WANG Pengcheng | | IEEE Transactions on0，，： | 1 |
| 10 | Locally adaptive metrics for clustering high dimensional data显示文摘 | Carlotta Domeniconi Dimitrios Gunopulos Sheng Ma Bojun Yan Muna Al-Razgan Dimitris Papadopoulos | 2007 | Data Mining and Knowledge Discovery2007，，1： | 1 |
| 11 | Locally Adaptive Metrics for Clustering High Dimensional Data 显示文摘 | Carlotta Domeniconi Dimitrios Gunopulos Sheng Ma Bojun Yan Muna Al-Razgan Dimitris Papado- poulos | 2007 | Data Mining and Knowledge Discovery2007，14，： | 1 |
| 12 | Locally adaptive metrics for clustering high dimensional data显示文摘 | Carlotta Domeniconi Dimitrios Gunopulos Sheng Ma Bojun Yan Muna Al-Razgan Dimitris Papadopoulos | 2007 | Data Mining and Knowledge Discovery2007，，1： | 1 |
| 13 | Expression analysis of the calcineurin B-like gene family in rice ( Oryza sativa L.) under environmental stresses显示文摘 | Zhimin Gu Bojun Ma Yan Jiang Zhiwei Chen Xuan Su Hongsheng Zhang | 2008 | Gene2008，，1： | 1 |
| 14 | Efficient selection of homozy-gous lines of hybrid rice restorer with the transgene Xa21 using test cross and PCR显示文摘The homozygous restorer lines with a single copy of the transgene Xa21 have been obtained from the progenies of transgenic Minghui63 and Yanhui559 plants through PCR marker-assisted selection and test cross. These homozygoustransgenic restorer lines can be used to breed hybrid rice with high resistance to bacterial leaf blight. | MA Bojun WANG Wenming ZOU Junhuang LIU Guoqing FANG Jin ZHU Lihuang ZHAI Wenxue | 2001 | Chinese Science Bulletin2001，46，2： | 0 |
| 15 | Residue and Safety Use of Emamectin Benzoate in Ginger显示文摘[Objective]The paper was to guide scientific and rational use of emamectin benzoate in ginger.[Method]A field experiment was conducted to determine residue and degradation dynamics and final residue of emamectin benzoate in ginger.The residue of emamectin benzoate in ginger was determined by high performance liquid chromatography-mass spectrometry(HPLC-MS/MS).[Result]The half-life of emamectin benzoate in ginger was 2.2-3.4 d.As 5%emamectin benzoate WDG was sprayed on ginger at 7.5 and 11.25 g·a.i/hm^2 for consecutive 1-2 times,the residues of emamectin benzoate in ginger harvested at 7,14 and 21 d post spraying were lower than the quantitative limit of the method(0.01 mg/kg),and were lower than the stipulation in Japan as well(0.1 mg/kg).[Conclusion]It is safe to use emamectin benzoate in ginger with the harvest interval of 7 d according to the recommended dose. | Feng Yizhi Zhang Aijuan Liang Lin Lin Dongsen Ren Yupeng Ma Xingang Li Wenping Zuo Bojun | 2019 | Plant Diseases and Pests2019，10，4： | 0 |
| 16 | A Large Acute Gastroenteritis Outbreak Associated with Both Campylobacter coli and Human Sapovirus—Beijing Municipality,China,2021显示文摘Summary What is already known about this topic?Campylobacter is a significant foodborne pathogen that leads to global outbreaks of acute gastroenteritis(AGE)usually affecting less than 30 individuals.Human sapovirus(HuSaV)is an enteric virus responsible for sporadic cases and outbreaks of AGE worldwide.In a study conducted in Beijing,HuSaV detection ranked second after norovirus.What is added by this report?We present a discussion of the first large-scale outbreak of AGE caused by both Campylobacter coli(C.coli)and HuSaV.The outbreak involved a total of 996 patients and exhibited two distinct peaks over a period of 17 days.Through case-control studies,we identified exposure to raw water from a secondary water supply system as a significant risk factor.Among 83 patients,49 samples tested positive for C.coli,39 samples tested positive for HuSaV,and 27 samples tested positive for both pathogens using real-time polymerase chain reaction detection.Furthermore,whole-genome sequencing of 17 C.coli isolates obtained from 17 patients revealed that all isolates belonged to a highly clonal strain of C.coli.What are the implications for public health practice?Outbreaks of AGE resulting from multiple pathogen infections warrant increased attention.This report emphasizes the significance of ensuring the safety of drinking water,particularly in secondary supply systems. | Lin Zou Ying Li Guilan Zhou Zhenzhou Huang Changyan Ju Chunyan Zhao Xiang Gao Bojun Zhen Ping Zhang Xiaochen Guo Jing Zhang Yang Zhang Bo Liu Shaolei Zhou Aixia Yan Ying Kang Yanchun Wang Hongmei Ma Xiaohui Li Maojun Zhang | 2023 | China CDC weekly2023，5，52： | 0 |
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