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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Relationship between HER-2 overexpression and brain metastasis in esophageal cancer patients显示文摘AIM:To study if HER-2 overexpression by locally advanced esophageal cancers increase the chance of brain metastasis following esophagectomy.METHODS:We retrospectively reviewed the medical records of esophageal cancer patients who underwent esophagectomy at University of Iowa Hospitals and Clinics between 2000 and 2010.Data analyzed consisted of demographic and clinical variables.The brain metastasis tissue was assayed for HER-2 overexpression utilizing the FDA approved DAKO Hercept Test.RESULTS:One hundred and forty two patients were reviewed.Median age was 64 years(36-86 years).Eighty eight patients(62%) received neoadjuvant chemoradiotherapy.Pathological complete and partial responses were achieved in 17(19%) and 71(81%) patients.Cancer relapsed in 43/142(30%) patients.The brain was the first site of relapse in 9/43 patients(21%,95% CI:10%-36%).HER-2 immunohistochemistry testing of the brain metastasis tissue showed that 5/9(56%) cases overexpressed HER-2(3+ staining).CONCLUSION:HER-2 overexpression might be associated with increased risk of brain metastasis in esophageal cancer patients following esophagectomy.Further studies will be required to validate this observation. | Taher Abu Hejleh Barry R DeYoung Eric Engelman Jeremy M Deutsch Bridget Zimmerman Thorvardur R Halfdanarson Daniel J Berg Kalpaj R Parekh William R Lynch Mark D Iannettoni Sudershan Bhatia Gerald Clamon | 2012 | World Journal of Gastrointestinal Oncology2012，4，5： | 5 |
| 3 | Risk of colon cancer in hereditary non-polyposis colorectal cancer patients as predicted by fuzzy modeling:Influence of smoking显示文摘瞄准:为了调查一个模糊逻辑模型是否能预言肤色,表面的癌症(CRC ) 风险由在世袭 non-polyposis 肤色吸表面的癌症(HNPCC ) 病人产生了。方法:从 Creighton 大学世袭癌症研究所登记的 340 个 HNPCC 失配修理(MMR ) 变化搬运人为当模特儿被选择。年龄依赖者曲线被产生阐明开发 CRC 的概率上的在基因变化(hMLH1 或 hMSH2 ) 之间的联合效果,性,和吸烟地位。结果:在男 hMSH2 变化搬运人的吸烟显著地增加的 CRC 风险(P <
0.05 ) 。hMLH1 变化为男性相对 hMSH2 变化搬运人扩充了 CRC 风险(P <
0.05 ) 。男性们非为 hMLH1 比女性有 CRC 的显著地更高的风险吸烟者(P <
0.05 ) , hMLH1 吸烟者(P <
0.1 ) 并且 hMSH2 吸烟者(P <
0.1 ) 。以在在男性的 hMSH2 的一种剂量依赖者方式的吸烟支持的 CRC (P <
0.05 ) 。有 hMSH2 变化的女性和与 hMLH1 组一起的两性仅仅在广泛的吸烟历史以后表明了吸烟效果(P <
0.05 ) 。结论:由在 HNPCC 病人吸烟的 CRC 提升依赖于基因变化,性和年龄。这些数据证明模糊建模可以启用临床的风险分数的明确的表达,从而允许 CRC 预防策略的 individualization。 | Rhonda M Brand David D Jones Henry T Lynch Randall E Brand Patrice Watson Ramesh Ashwathnayaran Hemant K Roy | 2006 | World Journal of Gastroenterology2006，12，28： | 5 |
| 4 | Interactions between biological processes, cultivation and soil structure显示文摘 | Lynch J M | 1984 | Plant Soil1984，76，： | 2 |
| 5 | Change of genetic architecture in response to sex显示文摘 | DENG H W LYNCH M | 1996 | Genetics1996，143，： | 1 |
| 6 | Heteronuclear 2D NMR studies of an engineered insulin monomer:assignment and characterization of the receptor-bingding surface by selective 2H and 13C labeling with application to protein design显示文摘 | Hua Q X Lynch C S | 1991 | Biochemistry1991，30，： | 1 |
| 7 | The origins of genome complexity显示文摘 | Lynch M Conery JS | 2003 | Science2003，302，5649： | 1 |
| 8 | Consequences of epilepsy in the developing brain:implications for surgical management显示文摘 | Stafstrom CE Lynch M Sutula TP | 2000 | Semin Pediatr Neurol2000，7，3： | 1 |
| 9 | A novel endothelial-derived lipase that modulatesHDL metabolism显示文摘 | Jaye M Lynch Kj Krawiec J | 1999 | Nat Genet1999，21，4： | 1 |
| 10 | Evaluation of periodontal regeneration following grafting intrabony defects with bio2oss collagen:a human histologic report显示文摘 | NEVINS ML CAMELO M LYNCH SE | 2003 | Int J Periodontics Restorative Dent2003，23，1： | 1 |
| 11 | Rate,molecular spectrum,and consequences of human mutation显示文摘 | Lynch M | 2010 | Proc Natl Acad Sci USA2010，1070，： | 1 |
| 12 | The similarity index and DNA fingerprinting显示文摘 | Lynch M | 1990 | Mol Biol Evol1990，7，： | 1 |
| 13 | The effect of celecoxib,a cyclooxygenase-2 inhibitor,in familial adenomatous polyposis显示文摘 | Steinbach G Lynch P M Philips R | 2000 | N Engl J Med2000，342，26： | 1 |
| 14 | Expression of estrogen receptor subtypes and ERβ isoforms in colon cancer显示文摘 | Campbell-Thompson M Lynch IJ Bhardwaj B | 2001 | Cancer Res2001，61，63： | 1 |
| 15 | Expression of a Bacillus thuringiensis cryIA (c)gene in transgenic peanut plants and its efficacy against lesser cornstalk borer显示文摘 | Singsit C Adang M J Lynch R E | 1997 | Transgenic Research1997，6，： | 1 |
| 16 | Human capital investments and productivity显示文摘 | Black S Lynch L M | 1996 | American Economic Review1996，86，2： | 1 |
| 17 | Caregiving Networks of Elderly Per?sons:Variation by Martital Status显示文摘 | Barrtee A E Lynch S M | 1999 | The Gerontologist1999，39，6： | 1 |
| 18 | Adenovirus 显示文摘 | Lynch JP 3rd Fishbein M Echavarria M | 2011 | Semin Respir Crit Care Med2011，32，4： | 1 |
| 19 | 查看详情显示文摘 | D W Yoon D E Gross V M Lynch J M Sessler B P Hay C H Lee | | 0，，： | 1 |
| 20 | The effect of the nitrification inhibitor dicyandiamide (DCD) on herbage production when applied at different times and rates in the autumn and winter显示文摘 | O'Connor PJ Hennessy D Brophy C O'Donovan M Lynch MB | 2012 | Agricultural Ecosystems & Environment2012，152，： | 1 |
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