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| 1 | Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction显示文摘 | Xiao-E Jia Ke Ma Tao Xu Lei Gao Shuang Wu Cong Fu Wenjuan Zhang Zhizhang Wang Kaiyu Liu Mei Dong Changbin Jing Chunguang Ren Zhiwei Dong Yi Chen Yi Jin Qiuhua Huang Xing Chang Min Deng Li Li Lingfei Luo Jun Zhu Yongjun Dang Hung-Chun Chang Leonard I Zon Yi Zhou Saijuan Chen Weijun Pan | 2015 | Cell Research2015，25，8： | 8 |
| 2 | Research and application of DY efficient flushing and spacer fluids in gas storage well cementing of changqing oilfield显示文摘 | Li Zhi Luo Changbin Hu Fuyuan | 2015 | Journal of Yangte University(Nat Sci Edit)2015，12，23： | 1 |
| 3 | N-methyl-D-aspartate receptor effects on p38 mitogen activated protein kinase and its role in a rat model of diabetic neuropathic pain显示文摘BACKGROUND:Activated N-methyl-D-aspartate(NMDA) receptor is involved in the formation of chronic neuropathic pain,and its antagonist,ketamine,exhibits effective amelioration of diabetic neuropathic pain(DNP).However,the mechanisms of NMDA receptor participation in the formation and maintenance of DNP remain poorly understood. OBJECTIVE:To evaluate the role NMDA receptor plays in DNP and effects on p38 mitogen activated protein kinase(p38 MAPK) in a rat model of DNP. DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Human Embryonic Stem Cell Research Institute of Yunyang Medical College Affiliated Taihe Hospital between July 2005 and September 2007. MATERIALS:Streptozotocin was provided by Sigma,USA;p38 MAPK inhibitor(SB203580) was provided by Shanghai KangChen Biotech,China;NMDA receptor antagonist(MK-801) was purchased from Shanghai Yope Biotech,China. METHODS:A total of 128 healthy,Wistar rats of clean grade,aged 3 months and weighing 180- 220 g,were randomly assigned to 4 groups:control,DNP model,p38 MAPK,and NMDA receptor. Each group contained 32 rats.DNP was established in all groups except for the control group by intraperitoneal injection of streptozocin(65 mg/kg).Subsequently,1 mg/kg SB203580 and 1 mg/kg MK-801 were injected once each week via intraperitoneal injection in the p38 MAPK and NMDA receptor groups,respectively. MAIN OUTCOME MEASURES:At the end of 2,4,6,and 8 weeks following streptozotocin injection, mechanical withdrawal threshold was measured in 8 animals from each group following von Frey filament stimulation.The rats were anesthetized and nerve conduction velocity of the left sciatic nerve was measured.Subsequently,the right sciatic nerve,the lumbar segment of the spinal cord, and dorsal root ganglia were removed from the L_(3-6) segment for microscopic examination.p38 MAPK expression was determined using immunohistochemistry and Western blot analysis. Expression of NMDA receptor 1 mRNA in dorsal root ganglion and spinal cord neurons was detected using RT-PCR. RESULTS:Mechanical withdrawal threshold and nerve conduction velocity were significantly reduced,and p38 MAPK and NMDA receptor 1 mRNA expression in the spinal cord and dorsal root ganglia were significantly increased,in the model,p38 MAPK,and NMDA receptor groups compared with the control group at all time points(P<0.05).At 4-8 weeks following successful DNP model establishment,SB203580 and MK-801 increased mechanical withdrawal threshold,accelerated nerve conduction velocity,and attenuated p38 MAPK expression,compared with the model group. The NMDA receptor group exhibited downregulated mRNA expression of NMDA receptor 1 compared with the model and p38 MAPK groups(P<0.05). CONCLUSION:NMDA receptor was highly expressed in the brains of DNP rats and was involved in DNP development via activation of the p38 MAPK signal pathway. | Changbin Ke Xiaoxia Huang Xianghong Luo Xiancheng Xu Chengming Qin Xianyu Wang Juying Liu | 2009 | Neural Regeneration Research2009，4，11： | 0 |
| 4 | High expression of BCL6 inhibits the differentiation and development of hematopoietic stem cells and affects the growth and development of chickens显示文摘Background:B-cell CLL/lymphoma 6(BCL6)is a transcriptional master regulator that represses more than 1200 potential target genes.Our previous study showed that a decline in blood production in runting and stunting syndrome(RSS)affected sex-linked dwarf(SLD)chickens compared to SLD chickens.However,the association between BCL6 gene and hematopoietic function remains unknown in chickens.Methods:In this study,we used RSS affected SLD(RSS-SLD)chickens,SLD chickens and normal chickens as research object and overexpression of BCL6 in hematopoietic stem cells(HSCs),to investigate the effect of the BCL6 on differentiation and development of HSCs.Results:The results showed that comparison of RSS-SLD chickens with SLD chickens,the BCL6 was highly expressed in RSS-SLD chickens bone marrow.The bone marrow of RSS-SLD chickens was exhausted and red bone marrow was largely replaced by yellow bone marrow,bone density was reduced,and the levels of immature erythrocytes in peripheral blood were increased.At the same time,the hematopoietic function of HSCs decreased in RSS-SLD chickens,which was manifested by a decrease in the hematopoietic growth factors(HGFs)EPO,SCF,TPO,and IL-3,as well as hemoglobinα1 and hemoglobinβexpression.Moreover,mitochondrial function in the HSCs of RSS-SLD chickens was damaged,including an increase in ROS production,decrease in ATP concentration,and decrease in mitochondrial membrane potential(ΔΨm).The same results were also observed in SLD chickens compared with normal chickens;however,the symptoms were more serious in RSS-SLD chickens.Additionally,after overexpression of the BCL6 in primary HSCs,the secretion of HGFs(EPO,SCF,TPO and IL-3)was inhibited and the expression of hemoglobinα1 and hemoglobinβwas decreased.However,cell proliferation was accelerated,apoptosis was inhibited,and the HSCs entered a cancerous state.The function of mitochondria was also abnormal,ROS production was decreased,and ATP concentration andΔΨm were increased,which was related to the inhibition of apoptosis of stem cells.Conclusions:Taken together,we conclude that the high expression of BCL6 inhibits the differentiation and development of HSCs by affecting mitochondrial function,resulting in impaired growth and development of chickens.Moreover,the abnormal expression of BCL6 might be a cause of the clinical manifestations of chicken comb,pale skin,stunted growth and development,and the tendency to appear RSS in SLD chickens. | Hongmei Li Bowen Hu Shang Hu Wen Luo Donglei Sun Minmin Yang Zhiying Liao Haohui Wei Changbin Zhao Dajian Li Meiqing Shi Qingbin Luo Dexiang Zhang Qinghua Nie Xiquan Zhang | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 0 |
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