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| 1 | 利用微卫星进行奶牛数量性状基因位点定位的研究显示文摘采用孙女设计法对德国奶牛的3个数量性状——产奶量、乳脂产量及乳蛋白产量进行了数量性状基因位点的定位研究。实验共有20个父系半同胞家系的1 130头种公牛,共测定了30对染色体上的229个微卫星,表型性状的育种值由孙代母牛估计得到。连锁分析采用多重遗传标记回归法,临界F值由置换实验法计算得到。统计检验发现在某些染色体上存在着一些显著的QTL’s区域,特别是14号染色体上某一区域对所有3个产奶性状都有极显著的影响。进一步证实了Coppieters和Ron等人的实验结果。 | 徐宁迎 H Thomsen N Reinsch C Looft E Kalm | 2000 | Acta Genetica Sinica2000，27，9： | 20 |
| 2 | 猪微卫星标记多重PCR扩增组合显示文摘采用多重PCR的方法,以快速扩增微卫星标记和节约试剂为目标,对其反应条件进行优化后,获得了46个扩增效果理想的微卫星标记多重PCR组合,其中30个为二重PCR,16个为三重PCR。实验结果表明,这些多重PCP反应的引物浓度为0.06~0.3μmol/L,Mg2+的浓度变化范围为1.5~3.0mmol/L,采用的PCR缓冲液的倍数为1.0、1.2、1.4或1.6,每个PCR反应聚合酶的用量在0.2和0.4U之间,退火温度及反应循环数分别为52~60℃和32~50℃。所有多重PCR进一步合并为17个可在ABI337测序仪上进行电泳的组合。 | 郭晓令 徐宁迎 LOOFT Christian REINSCH Norbert KAL M Ernst | 2004 | 遗传2004，26，1： | 6 |
| 3 | 用商品群作为参考系构建猪的微卫星连锁图谱显示文摘由 19头杂种公猪 [皮特兰× (皮特兰×汉普夏 ) ]、5 2头杂种母猪 [Leicoma× (大约克×长白 ) ]及其 332头后代组成的商品群作为参考系 ,选择 172个微卫星标记和 3个Ⅰ类标记 (RYR1、PIT1、PRKAG3)对参考系的个体进行遗传标记分型 ,应用CRIMAP(2 4 )构建猪的整个基因组微卫星连锁图谱。采用多重PCR方法对微卫星标记进行扩增 ,用ABI 377测序仪进行电泳分离 ,应用Genescan 3 0和Genotyper 2 0软件进行基因型检测。 3个Ⅰ类标记用PCR RFLP技术进行分型。CRIMAP程序分析表明 :所构建的猪常染色体性平均连锁图谱的总长度为 2 36 8 7cM ,X染色体的长度为 14 3 10cM ,遗传标记的平均间距为 16 3cM ,亲本的微卫星标记座位的杂合度平均为 0 70。此连锁图谱的构建将为商品猪群的生长、胴体、肉质以及繁殖性状的QTL扫描打下基础。 | 郭晓令 Looft Christian Reinsch Norbert Kalm Ernst | 2003 | Acta Genetica Sinica2003，30，7： | 5 |
| 4 | 利用商品猪群定位猪耳型QTL显示文摘由 19头杂种公猪 [皮特兰× (皮特兰×汉普夏 ) ]、5 2头杂种母猪 [Leicoma× (大约克×长白 ) ]及其 332头后代组成的商品群作为参考家系 ,选择 172个微卫星标记和 3个 1类标记 (RYR1、PRKAG3、PIT1)对参考家系的个体进行遗传标记分型 ,构建了猪的整个基因组微卫星连锁图谱。按照线性评分的方法 (竖耳 :1分 ;垂耳 :- 1分 ;半垂耳 :0分 )测定猪的耳型表型值。耳型测定值的平均值为 0 .2 3,标准差为 0 .82。应用最小二乘回归方法对耳型的QTL进行定位 ,结果仅在 6号染色体的末端 (Sw1881和Sw32 2 )之间以 1%基因组显著性水平检测出 1个耳型基因位点 ,而在其他染色体上即使 10 %染色体显著性水平上也没有发现QTL。 | 郭晓令 Looft Christian Reinsch Norbert Kalm Ernst | 2004 | Acta Genetica Sinica2004，31，8： | 2 |
| 5 | Eggshell and environmental bacteria contribute to the intestinal microbiota of growing chickens显示文摘Background: The initial intestinal microbiota acquired from different sources has profound impacts on animal health and productivity. In modern poultry production practices, the source(s) of the establishing microbes and their overall contribution during development of gastrointestinal tract communities are still unclear. Using fertilized eggs from two independent sources, we assessed the impact of eggshell-and environmental-associated microbial communities on the successional processes and bacterial community structure throughout the intestinal tract of chickens for up to 6 weeks post-hatch.Results: Culturing and sequencing techniques identified a viable, highly diverse population of anaerobic bacteria on the eggshell. The jejunal, ileal, and cecal microbial communities for the egg-only, environment-only, and conventionally raised birds generally displayed similar successional patterns characterized by increasing community richness and evenness over time, with strains of Enterococcus, Romboutsia, and unclassified Lachnospiraceae abundant for all three input groups in both trials. Bacterial community structures differed significantly based on trial and microbiota input with the exception of the egg-exposed and conventional birds in the jejunum at week 1 and the ileum at week 6. Cecal community structures were different based on trial and microbiota input source, and cecal short-chain fatty acid profiles at week 6 highlighted functional differences as well.Conclusion: We identified distinct intestinal microbial communities and differing cecal short-chain fatty acid profiles between birds exposed to the microbiota associated with either the eggshell or environment, and those of conventionally hatched birds. Our data suggest the eggshell plays an appreciable role in the development of the chicken intestinal microbiota, especially in the jejunum and ileum where the community structure of the eggshellonly birds was similar to the structure of conventionally hatched birds. Our data identify a complex interplay between the eggshell and environmental microbiota during establishment and succession within the chicken gut.Further studies should explore the ability of eggshell-and environment-derived microbes to shape the dynamics of succession and how these communities can be targeted through interventions to promote gut health and mitigate food-borne pathogen colonization in poultry. | Joel J.Maki Elizabeth A.Bobeck Matthew J.Sylte Torey Looft | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 2 |
| 6 | In-feed antibiotic effects on the swine intestinal microbiome显示文摘 | Looft T Johnson T A Allen H K et ol | 2012 | Proceedings of the National Academy of Sciences2012，109，5： | 1 |
| 7 | Bacteria, pha ges and pigs: the effects of in-feed antibiotics on the microbiome at different gut locations 显示文摘 | Looft T Allen H K Cantarel B L | 2014 | The ISME Journal2014，8，8： | 1 |
| 8 | Drip loss of case-ready meat and of premium cuts and their associations with earlier measured sample drip loss, meat quality and carcass traits in pigs显示文摘 | Otto G Roehe R Looft H | 2006 | Meat Science2006，72，4： | 1 |
| 9 | A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle 显示文摘 | MILAN D JEON J T LOOFT C | 2000 | Science2000，288，5469： | 1 |
| 10 | In-feed antibiotic effectson the swine intestinal microbiome显示文摘 | Looft TJohnson T A Allen H Kf | 2012 | Proc Natl Acad Sci USA2012，109，5： | 1 |
| 11 | Bovine β-defensins: Identification and characterization of novel bovine β-defensin genes and their expression in mammarygland tissue显示文摘 | Susanne Roosen Kay Exner Sven Paul Jens-Michael Schr?der Ernst Kalm Christian Looft | 2004 | Mammalian Genome2004，，10： | 1 |
| 12 | Carbadox has both temporary and lasting effects on the swine gut microbiota显示文摘 | Looft T Allen H K Casey T A | 2014 | Front Microbiol2014，，5： | 1 |
| 13 | Antibiotics in feed induceprophages in swine fecal microbiomes显示文摘 | Allen H K Looft T Bayles D 0 | 2011 | MBio2011，2，6： | 1 |
| 14 | Comparison of different methods for determination of drip loss and their relationships to meat quality and carcass characteristics in pigs显示文摘 | OTTO G ROEHE R LOOFT H | 2004 | Meat Sci- ence2004，68，3： | 1 |
| 15 | Rapid communication : detection and mapping of polymorphisms in the bovine lactoperoxidase (LPO) gene and in the glycosylation - dependent cell adhesion molecule 1 ( GlyCAM1 ) gene using fluorescent single - strand conformation polymorphism analysisl 显示文摘 | KARALL C LOOFT C KALM E | 1997 | J Anim Sci1997，75，： | 1 |
| 16 | In-feed antibiotic effects on the swine intestinal microbiome显示文摘 | Looft T Johnson TA Allen HK | 2012 | Proceedings of the National Academy of Sciences of the United States of America2012，109，5： | 1 |
| 17 | Collateral effects of antibiotics on mam-malian gut microbiomes显示文摘 | Looft T Allen H K | 2012 | Gut Microbes2012，3，5： | 1 |
| 18 | A mutation in PRKAG3 ac- coeiated with excess glycogen content in pig skeletal muscle显示文摘 | Milan D Jeon J T Looft C et o1 | 2000 | Science2000，288，5469： | 1 |
| 19 | Isolation and assign- ment of the UDP glucose pyrophosphorylase gene (UGP2) to porcine chromosome 3q21-->q22 by FISH and by analysis of somatic cell and radiation hybrid panels 显示文摘 | Looft C Paul S Thomsen P D | 2000 | Cytogenet Cell Genet2000，89，34： | 1 |
| 20 | A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle显示文摘 | Milan D Jeon J T Looft C | 2000 | Science2000，288，5469： | 1 |
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