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| 1 | Forced extension of P-selectin construct using steered molecular dynamics显示文摘P-selectin, a 70-nm-long cellular adhesive molecule, possesses elastic and extensible properties when neutrophils roll over the activated endotheliam of blood vessel in inflammatory reaction. Transient formation and dissociation of P-selectin/ligand bond on applied force of blood flow induces the extension of P-selectin and relevant ligands.Steered molecular dynamics simulations were performed to stretch a single P-selectin construct consisting of a lectin(Lec) domain and an epithelial growth factor (EGF)-Iike domain, where P-selectin construct was forced to extend in water with pulling velocities of 0.005-0.05 nm/ps and with constant forces of 1000--2500 pN respectively. Resulting force-extension profiles exhibited a dual-peak pattern on various velocities, while both plateaus and shoulders appeared in the extension-time profiles on various forces. The force or extension profiles along stretching pathways were correlated to the conformational changes, suggesting that the structural collapses of P-selectin Lec/EGF domains were mainly attributed to the burst of hydrogen bonds within the major β sheet of EGF domain and the disruptions of two hydrophobic cores of Lec domain. This work furthers the understanding of forced dissociation of P-selectin/ligand bond. | LüShouqin&LONGMian | 2004 | Chinese Science Bulletin2004，49，1： | 2 |
| 2 | Enhancement of rural domestic sewage treatment performance, and assessment of microbial community diversity and structure using tower vermifiltration显示文摘 | Longmian Wang Feihong Guo Zheng Zheng Xingzhang Luo Jibiao Zhang | 2011 | Bioresource Technology2011，，20： | 1 |
| 3 | Performance and mechanisms of a microbial-earthworm ecofilter for removing organic matter and nitrogen from synthetic domestic wastewater显示文摘 | Longmian Wang Zheng Zheng Xingzhang Luo Jibiao Zhang | 2011 | Journal of Hazardous Materials2011，，： | 1 |
| 4 | The current pollution status and control technology of the Taihu Lake Basin, Jiangsu Province, China显示文摘 | WANG Longmian ZHENG Zheng LUO Xingzhang | 2010 | International Journal of Environmental Studies2010，67，2： | 1 |
| 5 | Biostabilization enhancement of heavy metals during the vermiremedation of sewage sludge with passivant显示文摘 | Wang Longmian Zheng Zheng Zhang Yimin | 2013 | Journal of Hazardous Materials2013，，244245： | 1 |
| 6 | Overlying water fluoride concentrations influence dissolved organic matter composition and migration from pore water in sediment via bacterial mechanisms显示文摘Fluoride(F^(-))is widespread in aquatic environments;however,it is not clear whether the fluctuation of F^(-)concentrations in overlying lake water affects the composition and migration of dissolved organic matter(DOM)from sediment.A case study was presented in Sand Lake,China,and an experiment was conducted to analyze the influence of different F^(-)concentrations in overlying water on DOM characteristics.Diffusion resulted in similarities in DOM components between overlying and pore waters,and bacterial activities and enzyme variation resulted in differences between them.Higher F^(-)concentrations in overlying water resulted in a higher pH of pore water,which favored the enrichment of protein-like substances.Higher F^(-)concentrations caused lower DOM concentrations and lower maximum fluorescence intensities(Fmax)of protein-like components in pore water.The F^(-)concentrations had significantly negative correlations with Shannon indexes(P<0.05).Thiobacillus influenced the migration of tyrosinelike substances by decreasing the pH of pore water.Trichococcus and Fusibacter altered the Fmax of protein-like,humic-like,and fulvic-like substances.The F^(-)concentrations affected the DOM composition and migration due to the response of functional bacterial communities,which were positively correlated with the relative abundance of Thiobacillus and negatively correlated with the relative abundances of Trichococcus and Fusibacter.The high F^(-)concentrations influenced the biosynthesis and degradation of protein-like substances by shifting the abundances of the relevant enzymes.The results of this study may provide ideas for investigating DOM cycling under the influence of F^(-),especially in lakes with fluctuations in F^(-)concentrations. | Xiang Zhu Zibo Wang Yidan Luo Yushen Ma Zhipeng Xu Longmian Wang Fuquan Peng Qingqing Pang Yiping Li Fei He Bin Xu | 2022 | Environmental Science and Ecotechnology2022，，2： | 0 |
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