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| 1 | Nomogram to predict overall survival after gallbladder cancer resection in China显示文摘AIM To integrate clinically significant variables related to prognosis after curative resection for gallbladder carcinoma(GBC) into a predictive nomogram.METHODS One hundred and forty-two GBC patients who underwent curative intent surgical resection at Peking Union Medical College Hospital(PUMCH) were included. This retrospective case study was conducted at PUMCH of the Chinese Academy of Medical Sciences and Peking Union Medical College(CAMS & PUMC) in China from January 1, 2003 to January 1, 2018. The continuous variable carbohydrate antigen 19-9(CA19-9) was converted into a categorical variable(cCA19-9) based on the normal reference range. Stages 0 to IIIA were merged into one category, while the remaining stages were grouped into another category. Pathological grade X(GX) was treated as a missing value. A multivariate Cox proportional hazards model was used to select variables to construct a nomogram. Discrimination and calibration of the nomogram were performed via the concordance index(C-index) and calibration plots. The performance of the nomogram was estimated using the calibration curve. Receiver operating characteristic(ROC) curve analysis and decision curve analysis(DCA) were performed to evaluate the predictive accuracy and net benefit of the nomogram, respectively.RESULTS Of these 142 GBC patients, 55(38.7%) were male, and the median and mean age were 64 and 63.9 years, respectively. Forty-eight(33.8%) patients in this cohort were censored in the survival analysis. The median survival time was 20 months. A series of methods, including the likelihood ratio test and Akaike information criterion(AIC) as well as stepwise, forward, and backward analyses, were used to select the model, and all yielded identical results. Jaundice [hazard ratio(HR) = 2.9; 95% confidence interval(CI): 1.60-5.27], cCA19-9(HR = 3.2; 95%CI: 1.91-5.39), stage(HR = 1.89; 95%CI: 1.16-3.09), and resection(R)(HR = 2.82; 95%CI: 1.54-5.16) were selected as significant predictors and combined into a survival time predictive nomogram(C-index = 0.803; 95%CI: 0.766-0.839). High prediction accuracy(adjusted C-index = 0.797) was further verified via bootstrap validation. The calibration plot demonstrated good performance of the nomogram. ROC curve analysis revealed a high sensitivity and specificity. A high net benefit was proven by DCA.CONCLUSION A nomogram has been constructed to predict the overall survival of GBC patients who underwent radical surgery from a clinical database of GBC at PUMCH. | Yi Bai Zhi-Song Liu Jian-Ping Xiong Wei-Yu Xu Jian-Zhen Lin Jun-Yu Long Fei Miao Han-Chun Huang Xue-Shuai Wan Hai-Tao Zhao | 2018 | World Journal of Gastroenterology2018，24，45： | 25 |
| 2 | Features of Sandy Debris Flows of the Yanchang Formation in the Ordos Basin and Its Oil and Gas Exploration Significance显示文摘Sandy debris flow is a new genetic type of sand bodies,which has gained much attention in recent years and its corresponding theory is proved to be a significant improvement and even partial denial to the 'Bouma Sequence' and 'turbidite fan' deep-water sedimentary theories to some point. Oil exploration researchers are highly concerned with sandy debris flows for its key role in controlling oil and gas accumulation processes.In this article,by applying sandy debris flows theory and combining a lot work o... | LI Xiangbo CHEN Qilin LIU Huaqing WAN Yanrong WEI Lihua LIAO Jianbo LONG Liwen | 2011 | Acta Geologica Sinica(English Edition)2011，85，5： | 24 |
| 3 | Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer显示文摘Omega-3(ω-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of ω-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene. | PAN DengKe1, ZHANG Li1, ZHOU YanRong2, FENG Chong1, LONG Chuan1, LIU Xiao1, WAN Rong3, ZHANG Jian3, LIN AiXing3, DONG EnQiu4, WANG ShuChen4, XU HouGang4 & CHEN HongXing2 1 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2 Beijing Institute of Biotechnology, Beijing 100071, China 3 China Agricultural University, Beijing 100193, China 4 Yutian Breeding Swine Farms, Yutian 064104, China | 2010 | Science China(Life Sciences)2010，53，4： | 21 |
| 4 | Potential Toxicological and Cardiopulmonary Effects of PM2,5 Exposure and Related Mortality:Findings of Recent Studies Published during 2003-2013显示文摘INTRODUCTION Air pollution has become a serious environmental issue owing to its diverse harmful effects on the physical and biological environment.According to the Environmental Protection Agency(EPA)and the World Health Organization(WHO)[1-2],air pollution affects millions of people worldwide.Hundreds of thousands of deaths each year and a range of diseases, | Mohammed O.A.Mohammed SONG Wei Wei MA Wan Li LI Wen Long LI Yi Fan Afed Ullah Khan Mohammed A.E.M.Ibrahim Osman Adam Maarouf Alshebli A Ahmed John J.Ambuchi | 2016 | Biomedical and Environmental Sciences2016，29，1： | 16 |
| 5 | Multiple primary malignant tumors of upper gastrointestinal tract:A novel role of ^(18)F-FDG PET/CT显示文摘AIM: To evaluate the capacity of 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) for detecting multiple primary cancer of upper gastrointestinal (UGI) tract. METHODS: Fifteen patients (12 without cancer histories and 3 with histories of upper GI tract cancer) were investigated due to the suspicion of primary cancer of UGI tract on X-ray barium meal and CT scan. Subsequent whole body 18F-FDG PET/CT scan was carried out for initial staging or restaging. All the patients were fi nally confi rmed by endoscopic biopsy or surgery. The detection rate of multiple primary malignant cancers was calculated based on 18F-FDG PET/CT and endoscopic examinations.RESULTS: 18F-FDG PET/CT scan was positive in 32 suspicious lesions, 30/32 were true positive primary lesions, and 2/32 were false positive. In 15 suspicious lesions with negative 18F-FDG PET/CT scan, 12/15 were true negative and 3/15 were false negative. Among the 15 patients, 12 patients had 29 primary synchronous tumors confirmed by pathology, including 8 cases of esophageal cancers accompanied with gastric cancer and 4 of hypopharynx cancers with esophageal cancer. The other 3 patients had 4 new primary metachronous tumors, which were multiple primary esophageal cancers. PET/CT imaging detected local lymph node metastases in 11 patients. Both local lymph node metastases and distant metastases were detected in 4 patients. On a per-primary lesion basis, the sensitivity, specificity, accuracy, negative predictive value and positive predictive value of 18F-FDG PET/CT for detecting multiple primary cancer of UGI tract were 90.9%, 85.7%, 89.4%, 80% and 93.7%, respectively. CONCLUSION: The whole body 18F-FDG PET/CT may play an important role in evaluating the multiple primary malignant tumors of UGI tract cancer. | Long Sun Yong-Hong Sun Long Zhao Zuo-Ming Luo Hua Wu Ying Wan Qin Lin | 2010 | World Journal of Gastroenterology2010，16，31： | 14 |
| 6 | Effects of Nitrogen Fertilizer Level on Chlorophyll Fluorescence Characteristics in Flag Leaf of Super Hybrid Rice at Late Growth Stage显示文摘To compare the effects of slow-release nitrogen fertilizer at six different levels on the flag leaf chlorophyll fluorescence characteristics of super hybrid rice,a field fertilization experiment was conducted with super hybrid rice Y Liangyou 1 as a test material.The photosynthetic electron transport rate (ETR),effective quantum yield (EQY),photochemical quenching coefficient (q P),and non-photochemical quenching coefficient (NPQ) of flag leaves were measured at the initial heading,full heading,10 d after full heading and 20 d after full heading stages.Results showed that the values of ETR,EQY and q P increased with rice development from initial heading to 20 d after full heading,whereas the NPQ decreased.During the measured stages,ETR,EQY and q P increased initially and then decreased as nitrogen application amount increased,but they peaked at different nitrogen fertilizer levels.The maximum ETR and EQY values appeared at the treatment of 135 kg/hm 2 N.In conclusion,the optimum nitrogen amount for chlorophyll fluorescence characteristics of super hybrid rice was 135 180 kg/hm 2. | LONG Ji-rui MA Guo-hui WAN Yi-zheng SONG Chun-fang SUN Jian QIN Rui-jun | 2013 | Rice science2013，20，3： | 11 |
| 7 | Selenium Supplementation Alleviates Autoimmune Thyroiditis by Regulating Expression of Th1/Th2 Cytokines显示文摘Selenium (Se) is an essential trace element.Autoimmune thyroid diseases (AITD) are destructive inflammatory or anti-receptor autoimmune diseases characterized by reactivity to self-thyroid antigens.However,the effects of Se on the cytokines in AITD are still unclear.So we researched the role of Selenium (Se) and Th1/Th2 cytokine productions in the pathogenesis of autoimmune thyroid diseases (AITD).The data shown that high Se intake reduced the autoantibody titers and alleviated the | TAN Long SANG Zhong Na SHEN Jun WU Yun Tang YAO Zhai Xiao ZHANG Jin Xiang ZHAO Na ZHANG Wan Qi | 2013 | Biomedical and Environmental Sciences2013，26，11： | 10 |
| 8 | Mechanical Properties and Microstructure of 6082-T6 Aluminum Alloy Joints by Self-support Friction Stir Welding显示文摘The majority of this research has concentrated on developing the self-support friction stir welding(SSFSW) tool which consists of a big concave upper shoulder and a small convex lower shoulder, and procedures for making reliable welds in aluminum hollow extrusion. The 5-mm-thick 6082-T6 aluminum alloy was self-support friction stir welded at a constant tool rotation speed of 800 r/min. The effect of welding speed on microstructure and mechanical properties was investigated. The results of transverse tensile test indicated that the tensile strength of joints increased and the elongation decreased with increasing welding speed. The whole values of microhardness of SSFSW joints increased with increasing welding speed from 10 to 200 mm/min. The defectfree joints were obtained at lower welding speeds and the tensile fracture was located at the heat-affected zone(HAZ) adjacent to the thermo-mechanically affected zone(TMAZ) on the advancing side. The investigation of the flow pattern of the softened metal around the SSFSW tool revealed that the flow pattern of the softened metal was driven by two shoulders and the stir pin. The failure of specimens in tension presented the ductile fracture mode. | Long Wan Yongxian Huang Weiqiang Guo Shixiong Lv Jicai Feng | 2014 | Journal of Materials Science & Technology2014，30，12： | 10 |
| 9 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 10 | Effect of ZrO_2 Content on the Properties of Diamond Grinding Wheel Vitrified Bond显示文摘The effect of ZrO2 content on the performance of vitrified bond was discussed. The results showed that when the ZrO2 content was less than 2 mol%, Zr4+ could enter into the glassy network as mending nets and agglomeration composition. In this case, with the increasing of ZrO2, the vitrified bond's bending strength increased and the expansion coefficient decreased. It was also found that the wetting angle between the vitrified bond and diamond got small because Zr4+ was enriched on diamond surface. When ZrO2 content was more than 2 mol%, a part of ZrO2 existing in vitrified bond as ZrO2 particles played the role in nucleating agents and promoting the separation of spodumene. When ZrO2 content was 3 mol%, the bending strength of the vitrified bond reached its maximum of 128 MPa and the wetting angle with the diamond reached its minimum of 37.6°. | 刘小磐 QIAO Ang WAN Long HU Weida SONG Dongdong LI Zheng YIN Zhongjie 潘瑞琨 | 2014 | Journal of Wuhan University of Technology(Materials Science)2014，29，1： | 7 |
| 11 | Genetic Analysis of Two Weak Dormancy Mutants Derived from Strong Seed Dormancy Wild Type Rice N22 (Oryza sativa)显示文摘Two weak dormancy mutants,designated Q4359 and Q4646,were obtained from the rice cultivar N22 after treatment with 400 Gy 60Co gamma-radiation.Compared to the N22 cultivar,the dormancy of the mutant seeds was more readily broken when exposed to a period of room temperature storage.The mutants also showed a reduced level of sensitivity to abscisic acid compared to the N22 cultivar,although Q4359 was more insensitive than Q4646.A genetic analysis indicated that in both mutants,the reduced dormancy trait was caused by a single recessive allele of a nuclear gene,but that the mutated locus was different in each case.The results of quantitative trait locus(QTL)mapping,based on the F2 population from Q4359 x Nanjing35,suggested that Q4359 lacks the QTL qSdn-1 and carries a novel allele at QTL qSdn-9, while a similar analysis of the Q4646 x Nanjing35 F2 population suggested that Q4646 lacks QTL qSdn-5, both qSdn-1 and qSdn-5 are major effect seed dormancy QTL in N22.Therefore,these two mutants were helpful to understand the mechanism of seed dormancy in N22. | Bingyue Lu KunXie Chunyan Yang Long Zhang Tao Wu Xi Liu Ling Jiang Jianmin Wan | 2011 | Journal of Integrative Plant Biology2011，53，5： | 7 |
| 12 | MmNet:Identifying Mikania micrantha Kunth in the wild via a deep Convolutional Neural Network显示文摘Mikania micrantha Kunth is an invasive alien weed and known as a plant killer around the world.Accurately and rapidly identifying M.micrantha in the wild is important for monitoring its growth status,as this helps management officials to take the necessary steps to devise a comprehensive strategy to control the invasive weed in the identified area.However,this approach still mainly depends on satellite remote sensing and manual inspection.The cost is high and the accuracy rate and efficiency are low.We acquired color images of the monitoring area in the wild environment using an Unmanned Aerial Vehicle(UAV)and proposed a novel network-MmNet-based on a deep Convolutional Neural Network(CNN)to identify M.micrantha in the images.The network consists of AlexNet Local Response Normalization(LRN),along with the GoogLeNet and continuous convolution of VGG inception models.After training and testing,the identification of 400 testing samples by MmNet is very good,with accuracy of 94.50%and time cost of 10.369 s.Moreover,in quantitative comparative analysis,the proposed MmNet not only has high accuracy and efficiency but also simple construction and outstanding repeatability.Compared with recently popular CNNs,MmNet is more suitable for the identification of M.micrantha in the wild.However,to meet the challenge of wild environments,more M.micrantha images need to be acquired for MmNet training.In addition,the classification labels need to be sorted in more detail.Altogether,this research provides some theoretical and scientific basis for the development of intelligent monitoring and early warning systems for M.micrantha and other invasive species. | QIAO Xi LI Yan-zhou SU Guang-yuan TIAN Hong-kun ZHANG Shuo SUN Zhong-yu YANG Long WAN Fang-hao QIAN Wan-qiang | 2020 | Journal of Integrative Agriculture2020，19，5： | 7 |
| 13 | Certificateless key-insulated signature without random oracles显示文摘Leakage of the private key has become a serious problem of menacing the cryptosystem security. To reduce the underlying danger induced by private key leakage, Dodis et al.(2003) proposed the first key-insulated signature scheme. To handle issues concerning the private key leakage in certificateless signature schemes, we devise the first certificateless key-insulated signature scheme. Our scheme applies the key-insulated mechanism to certificateless cryptography, one with neither certificate nor key escrow. We incorporate Waters (2005)’s signature scheme, Paterson and Schuldt (2006)’s identity-based signature scheme, and Liu et al.(2007)’s certificateless signature scheme to obtain a certificateless key-insulated signature scheme. Our scheme has two desirable properties. First, its security can be proved under the non-pairing-based generalized bilinear Diffie-Hellman (NGBDH) conjecture, without utilizing the random oracle model; second, it solves the key escrow problem in identity-based key-insulated signatures. | Zhong-mei WAN Xue-jia LAI Jian WENG Sheng-li LIU Yu LONG Xuan HONG | 2009 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2009，10，12： | 6 |
| 14 | Research on testing method of resin sand high temperature compressive strength显示文摘High temperature compressive strength is one of the most important performances of resin sand; its value directly concerns the quality of castings. In order to seek the best testing method of resin sand high temperature compressive strength, a self-developed instrument was used to carry out experiments, and the sample shape and size were designed and studied. The results show that a hollow cylinder sample can reflect the strength difference of different resin sands better than a solid cylinder sample, and its data is stable. The experiments selected Φ20/5×30 mm as the size of the hollow cylinder samples. The high temperature compressive strengths of phenol-formaldehyde resin coated sand, furan resin self-setting sand, and TEA resin sand were each tested. For the resin sand used for cast steel and cast iron, 1,000 oC was selected as the test temperature; for the resin sand used for cast non-ferrous al oy, 800 oC was selected as the test temperature; and for all the resin sand samples, 1 min was selected as the holding time. This testing method can truthfully reflect the high temperature performance of three kinds of resin sand; it is reproducible, and the variation coefficients of test values are under 10%. | Peng Wan Luan-cai Li Long Zhang Wen-qing Wang | 2016 | China Foundry2016，13，5： | 6 |
| 15 | Cyanidin-3-O-galactoside and Blueberry Extracts Supplementation Improves Spatial Memory and Regulates Hippocampal ERK Expression in Senescence-accelerated Mice显示文摘Objective To investigate whether the antioxidation and the regulation on the Extracellular Regulated Protein Kinases(ERK) signaling pathway are involved in the protective effects of blueberry on central nervous system. Methods 30 Senescence-accelerated mice prone 8(SAMP8) mice were divided into three groups and treated with normal diet, blueberry extracts(200 mg/kg·bw/day) and cyaniding-3-O-galactoside(Cy-3-GAL)(50 mg/kg·bw/day) from blueberry for 8 weeks. 10 SAMR1 mice were set as control group. The capacity of spatial memory was assessed by Passive avoidance task and Morris water maze. Histological analyses on hippocampus were completed. Malondialdehyde(MDA) levels, Superoxide Dismutase(SOD) activity and the expression of ERK were detected. Results Both Cy-3-GAL and blueberry extracts were shown effective functions to relieve cellular injury, improve hippocampal neurons survival and inhibit the pyramidal cell layer damage. Cy-3-GAL and blueberry extracts also increased SOD activity and reduced MDA content in brain tissues and plasma, and increased hippocampal phosphorylated ERK(p-ERK) expression in SAMP8 mice. Further more, the passive avoidance task test showed that both the latency time and the number of errors were improved by Cy-3-GAL treatment, and the Morris Water Maze test showed significant decreases of latency were detected by Cy-3-GAL and blueberry extracts treatment on day 4. Conclusion Blueberry extracts may reverse the declines of cognitive and behavioral function in the ageing process through several pathways, including enhancing the capacity of antioxidation, altering stress signaling. Cy-3-GAL may be an important active ingredient for these biological effects. | TAN Long YANG Hong Peng PANG Wei LU Hao HU Yan Dan LI Jing LU Shi Jun ZHANG Wan Qi JIANG Yu Gang | 2014 | Biomedical and Environmental Sciences2014，27，3： | 6 |
| 16 | HIGH-TEMPERATURE AC MAGNETIC PROPERTIES OF NANOCRYSTALLINE FINEMET ALLOYS WITH Co ADDITION显示文摘高温度的交流磁性为(Fe0.5Co0.5 ) 73.5Cu1Nb3 Si13.5B9 合金在各种各样的温度退火了用一个阻抗分析器被调查了。退火的温度 Ta 在合金的高温度的交流磁性上有明显的效果。Hopkinson 山峰仅仅在非结晶的合金出现什么时候 Ta ≤ 703K。作为在 733K 上面的 Ta 增加,复杂起始的渗透β i 的真实部分“逐渐地下降,β i ”在高温度范围与温度更慢慢地减少。高温度(大约 750K ) 为合金的交流起始的渗透在 Ta=763K 退火了被发现了维持 950 的稳定的高价值直到 1 × 105Hz 的频率。为在 Fe73.5Cu1Nb3Si13.5B9 合金的 Fe 的公司的部分替换导致增加在无定形相的居里温度的 110K 附近,比包含 Ge Finemet 高得多合金。 | Y.H. Ding X. Liu S.S. Zhao Y. Long R.C. Ye Y.Q. Chang F.R. Wan | 2007 | Acta Metallurgica Sinica(English Letters)2007，20，5： | 6 |
| 17 | Multi-object approach and its appfication to adaptive water management under climate change显示文摘This paper addresses issues on adaptive water management under the impact of climate change.Based on a set of comprehensive indicators of water system,a decision making approach of multi-objects is developed and applied to quantify water adaptive management for the demands of water sustainable use,water environmental protection and eco-water requirement under the climate change.For this study in China,two key indicators are proposed,namely(1) the water resources vulnerability(V) that was represented by integrated sensitivity(S) and resilience(C) of climate change impact on water resources,and(2) the sustainability of socio-economy and water environment,marked by DD,that is integrated scaler of socio-economic development(EG) based on the amount of GDP and the water environment and relative eco-system quality(LI).To find a reasonable solution for adaptive water management,a multi-objective decision making model of adaptive water management is further developed and the multi-objective model was transformed into an integrated single optimization model through developing an integrated measure function,called as VDD=DD/V.This approach has been applied to adaptive water resources planning and management for case study of China with new policy,called as the strict management of water resources based on three red line controls,i.e.,the control of total water use by the total water resources allocation,the control of lower water use efficiency by the water demand management and the control of the total waste water load by water quality management in the Eastern China Monsoon Region that covers major eight big river basins including Yangtze River,Yellow River,Haihe River and Huaihe River.It is shown that the synthetic representation of water resource vulnerability and socio-economic sustainability by the integrated objective function(VDD) and integrated decision making model are workable and practicable.Adaptive management effect of the criterion compliance rate and water use efficiency are more appreciable through new water policy of the three red line controls,which can reduce 21.3% of the water resources vulnerability(V) and increase 18.4% of the sustainability of socioeconomy and water environment(DD) for the unfavorable scenario of climate change in 2030. | HONG Si XIA Jun CHEN Junxu WAN Long NING Like SHI Wei | 2017 | Journal of Geographical Sciences2017，27，3： | 5 |
| 18 | Functional distinction of rat liver natural killer cells from spleen natural killer cells under normal and acidic conditions in vitro显示文摘BACKGROUND: The microenvironment within solid tumors has often been shown to exhibit an acidic extracellular pH. Although the morphologic and functional differences in natural killer (NK) cells of the liver and spleen have been reported previously under physiological conditions, the difference under acidic conditions is still unclear. This study was to investigate the differences in the morphological and functional characteristics between rat liver and spleen NK cells under normal and acidic conditions in vitro. METHODS: Liver and spleen NK cells were isolated and purified from Sprague-Dawley rats by density gradient centrifugation and the Dynabeads FlowComp TM Flexi system, and stimulated for 4 days with or without IL-2 or treated with low pH or control for different times. Morphology was examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cell death and proliferation assays were performed by flow cytometry, IFN-γ production was tested by ELISA, and cytotoxic activity was evaluated by lactate dehydrogenase (LDH) release assay. RESULTS: Liver NK cells had significantly higher levels of cytotoxic activity than spleen NK cells under normal and acidic conditions, and the maximum difference was observed at pH 5.6. Further analysis revealed that the cytotoxic activity of NK cells was correlated with morphology, cell death, proliferative activity and IFN-γ production. By TEM, liver NK cells contained a greater number of electron-dense granules per cell at pH 5.6.Moreover, a modest elevation of cell death and reduction of proliferation of liver NK cells occurred within a range of 5.6-7.2. Interestingly, an acidic extracellular pH only marginally, and not significantly, suppressed IFN-γ production by liver NK cells. CONCLUSION: The sharp morphological and functional differences shown by the two types of NK cells in vitro indicate that liver NK cells are unexpectedly resistant to pH shock. | Li-Hong Lv, Jian-Dong Yu, Guo-Lin Li, Tian-Zhu Long, Wei Zhang, Ya-Jin Chen, Jun Min and Yun-Le Wan Department of Hepatobiliary Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Department of Breast Surgery, Guangzhou Women and Children’s Medical Center Guangzhou 510120, China Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 5 |
| 19 | Consensus on the digestive endoscopic tunnel technique显示文摘With the digestive endoscopic tunnel technique(DETT), many diseases that previously would have been treated by surgery are now endoscopically curable by establishing a submucosal tunnel between the mucosa and muscularis propria(MP). Through the tunnel, endoscopic diagnosis or treatment is performed for lesions in the mucosa, in the MP, and even outside the gastrointestinal(GI) tract.At present, the tunnel technique application range covers the following:(1)Treatment of lesions originating from the mucosal layer, e.g., endoscopic submucosal tunnel dissection for oesophageal large or circular early-stage cancer or precancerosis;(2) treatment of lesions from the MP layer, per-oral endoscopic myotomy, submucosal tunnelling endoscopic resection, etc.; and(3) diagnosis and treatment of lesions outside the GI tract, such as resection of lymph nodes and benign tumour excision in the mediastinum or abdominal cavity. With the increasing number of DETTs performed worldwide, endoscopic tunnel therapeutics, which is based on DETT, has been gradually developed and optimized. However, there is not yet an expert consensus on DETT to regulate its indications, contraindications, surgical procedure, and postoperative treatment.The International DETT Alliance signed up this consensus to standardize the procedures of DETT. In this consensus, we describe the definition, mechanism,and significance of DETT, prevention of infection and concepts of DETTassociated complications, methods to establish a submucosal tunnel, and application of DETT for lesions in the mucosa, in the MP and outside the GI tract(indications and contraindications, procedures, pre-and postoperative treatments, effectiveness, complications and treatments, and a comparison between DETT and other operations). | Ning-Li Chai Hui-Kai Li En-Qiang Linghu Zhao-Shen Li Shu-Tian Zhang Yu Bao Wei-Gang Chen Philip WY Chiu Tong Dang Wei Gong Shu-Tang Han Jian-Yu Hao Shui-Xiang He Bing Hu1 Bing Hu2 Xiao-Jun Huang Yong-Hui Huang Zhen-Dong Jin Mouen A Khashab James Lau Peng Li Rui Li De-Liang Liu Hai-Feng Liu Jun Liu Xiao-Gang Liu Zhi-Guo Liu Ying-Cai Ma Gui-Yong Peng Long Rong Wei-Hong Sha Pateek Sharma Jian-Qiu Sheng Shui-Sheng Shi Dong Wan Seo Si-Yu Sun Gui-Qi Wang Wen Wang Qi Wu Hong Xu Mei-Dong Xu Ai-Ming Yang Fang Yao Hong-Gang Yu Ping-Hong Zhou Bin Zhang Xiao-Feng Zhang Ya-Qi Zhai | 2019 | World Journal of Gastroenterology2019，25，7： | 5 |
| 20 | SARS-CoV-2 Omicron variant:recent progress and future perspectives显示文摘Since the outbreak of the coronavirus disease 2019(COVID-19)pandemic,there have been a few variants of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),one of which is the Omicron variant(B.1.1.529).The Omicron variant is the most mutated SARS-CoV-2 variant,and its high transmissibility and immune evasion ability have raised global concerns.Owing to its enhanced transmissibility,Omicron has rapidly replaced Delta as the dominant variant in several regions.However,recent studies have shown that the Omicron variant exhibits reduced pathogenicity due to altered cell tropism.In addition. | Yao Fan Xiang Li Lei Zhang Shu Wan Long Zhang Fangfang Zhou | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 4 |
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