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| 1 | Identification of maize seed varieties based on near infrared reflectance spectroscopy and chemometrics显示文摘False seeds can often be seen in the maize seed market,leading to a serious decline in maize yield.Those existing variety identification methods are expensive,time consuming,and destructive to seeds.The aim of this study is to develop a cheap,fast and non-destructive method which can robustly identify large amounts of maize seed varieties based on near-infrared reflectance spectroscopy(NIRS)and chemometrics.Because it is difficult to establish models for every variety in the market,this study mainly investigated the performance of models based on a large number of samples(more than 40 major varieties in the market).The reflectance spectra of maize seeds were collected by two modes(bulk kernels mode and single kernel mode).Both collection modes can be applied to identification,but only the single kernel mode can be applied to purity sorting.The spectra were pretreated with smoothing,the first derivative and vector normalization;and then principal component analysis(PCA),linear discriminant analysis(LDA)and biomimetic pattern recognition(BPR)were applied to establish identification models.The environmental factors such as producing areas and years have a significant influence on the performance of the models.Therefore,the method to improve the robustness of the models was investigated in this study.New indexes(correct acceptance degree(CAD),correct rejection degree(CRD)and correct degree(CD))were defined to analyze the performance of the models more accurately.Finally,the models obtained a mean correct discrimination rate of over 90%,and exhibited robust properties for samples harvested from different areas and years.The results showed that NIR technology combined with chemometrics methods such as PCA,LDA,and BPR could be a suitable and alternative technique to identify the authenticity of maize seed varieties. | Yongjin Cui Lanjun Xu Dong An Zhe Liu Jiancheng Gu Shaoming Li Xiaodong Zhang Dehai Zhu | 2018 | International Journal of Agricultural and Biological Engineering2018，11，2： | 3 |
| 2 | 0.35%THz pulse conversion efficiency achieved by Ti:sapphire femtosecond laser filamentation in argon at 1 kHz repetition rate显示文摘In this study,an optical setup for generating terahertz(THz)pulses through a two-color femtosecond laser filament was carefully designed to achieve a precise overlap of two-color laser pulses in space and time.β-barium borate(BBO),α-BBO,and a dual-wavelength plate were used to compensate the phase delay of the two-color lasers.Tilting ofα-BBO could further realize the precise spatial overlap of the two beams by counteracting the walk-off effect.The maximum out-put THz pulse energy reached 21μJ in argon gas when using a commercial Ti:sapphire laser with a pulse energy of 6 mJ at a 1 kHz repetition rate.The corresponding conversion efficiency exceeded 0.35%. | Zhiqiang Yu Nan Zhang Jianxin Wang Zijie Dai Cheng Gong Lie Lin Lanjun Guo Weiwei Liu | 2022 | Opto-Electronic Advances2022，5，9： | 2 |
| 3 | Anti-Correlated Plasma and THz Pulse Generation during Two-Color Laser Filamentation in Air显示文摘The THz generation efficiency and the plasma density generated by a filament in air have been found anti-correlated when pumped by 800 nm+1600 nm two-color laser field.The plasma density near zero delay of two laser pulses has a minimum value,which is opposite to the trend of THz generation eficiency and contradicts common sense.The lower plasma density cannot be explained by the static tunneling model according to the conventional photocurrent model,but it might be attributed to the electron trapping by the excited states of nitrogen molecule.The present work also clarifies the dominant role of the drifting velocity accelerated by the two-color laser field during the THz pulse generation process.The results promote our understanding on the optimization of the THz generation efficiency by the two-color laser filamentation. | Zhiqiang Yu Lu Sun Nan Zhang Jianxin Wang Pengfei Qi Lanjun Guo Quan Sun Weiwei Liu Hiroaki Misawa | 2022 | Ultrafast Science2022，，6： | 2 |
| 4 | Microwave-assisted acid-induced formation of linker vacancies within Zr-based metal organic frameworks with enhanced heterogeneous catalysis显示文摘Herein,we report a microwave-assisted acid-induced post-treatment method for the formation of linker vacancies within Zr-based metal organic frameworks(Zr-MOFs).The number of linker vacancies can be easily regulated with this method by changing the concentration of the HCl solution and the duration of microwave irradiation.The optimized defective UiO-66 showed higher linker defects with a higher specific surface area and thermal stability.The results of the catalytic cyclization of citronella showed that the Zr-MOFs with more defects exhibited enhanced catalytic performance.This work may provide a new method for the creation of defective MOFs with high activity and stability. | Yu Liang Chenhui Li Lanjun Chen Jia Huo Mohammed Loubidi Yangyang Zhou Yanbo Liu | 2021 | Chinese Chemical Letters2021，32，2： | 1 |
| 5 | The role of human ADA2a in the regulation of p53 acetylation and stability显示文摘The tumor suppressor protein p53 is a well-known transcription factor that functions as a critical component of the genotoxic stress response via regulating the expression of effector proteins that control cellular fate following DNA damage. The human p300/CBP-associated factor(PCAF)-containing histone acetyltransferase(HAT) complex is important for the stability and activity of p53 via its acetylation. The human homolog of yeast alteration/deficiency in activation 2a(ADA2a) is a stable component of the human PCAF-containing HAT complex. In this study,we demonstrated that p53 and hADA2a physically interact with each other in human HEK 293T cells. Using overexpression and small interfering RNA-mediated knockdown,we demonstrated that hADA2a stabilizes p53 via promoting its acetylation at lysine 320 - a PCAF-dependent acetylation site. Furthermore,hADA2a can potentiate the transcriptional activity of p53 at the BAX and p21 promoters to induce cell apoptosis and cell cycle arrest. Overall,our results establish that hADA2a,a component of the PCAF-containing histone acetyltransferase co-activator complex,is a mediator of acetylation-dependent stabilization and activation of p53 in mammalian cells. | HUANG Jing ZHANG Li XIAO Lin XU LanJun HU FanLei SHAO WenWei LIU Wei MO XiaoNing SHI TaiPing QIU XiaoYan | 2011 | Chinese Science Bulletin2011，56，4： | 1 |
| 6 | Effect of sulfamethazine on the horizontal transfer of plasmid-mediated antibiotic resistance genes and its mechanism of action显示文摘As a new type of environmental pollutant,antibiotic resistance genes(ARGs)pose a huge challenge to global health.Horizontal gene transfer(HGT)represents an important route for the spread of ARGs.The widespread use of sulfamethazine(SM2)as a broad-spectrum bacteriostatic agent leads to high residual levels in the environment,thereby increasing the spread of ARGs.Therefore,we chose to study the effect of SM2 on the HGT of ARGs mediated by plasmid RP4 from Escherichia coli(E.coli)HB101 to E.coli NK5449 as well as its mechanism of action.The results showed that compared with the control group,SM2 at concentrations of 10 mg/L and 200 mg/L promoted the HGT of ARGs,but transfer frequency decreased at concentrations of 100 mg/L and 500 mg/L.The transfer frequency at 200 mg/L was 3.04×10^(−5),which was 1.34-fold of the control group.The mechanism of SM2 improving conjugation transfer is via enhancement of the mRNA expression of conjugation genes(trbBP,trfAP)and oxidative stress genes,inhibition of the mRNA expression of vertical transfer genes,up regulation of the outer membrane protein genes(ompC,ompA),promotion of the formation of cell pores,and improvement of the permeability of cell membrane to promote the conjugation transfer of plasmid RP4.The results of this study provide theoretical support for studying the spread of ARGs in the environment. | Xiaojing Yan Wenwen Liu Shengfang Wen Lanjun Wang Lusheng Zhu Jun Wang Young Mo Kim Jinhua Wang | 2023 | Journal of Environmental Sciences2023，，5： | 1 |
| 7 | SUMOylation-triggered ALIX activation modulates extracellular vesicles circTLCD4-RWDD3 to promote lymphatic metastasis of non-small cell lung cancer显示文摘Lymph node(LN)metastasis is one of the predominant metastatic routes of non-small cell lung cancer(NSCLC)and is considered as a leading cause for the unsatisfactory prognosis of patients.Although lymphangiogenesis is well-recognized as a crucial process in mediating LN metastasis,the regulatory mechanism involving lymphangiogenesis and LN metastasis in NSCLC remains unclear.In this study,we employed high-throughput sequencing to identify a novel circular RNA(circRNA),circTLCD4-RWDD3,which was significantly upregulated in extracellular vesicles(EVs)from LN metastatic NSCLC and was positively associated with deteriorated OS and DFS of patients with NSCLC from multicenter clinical cohort.Downregulating the expression of EV-packaged circTLCD4-RWDD3 inhibited lymphangiogenesis and LN metastasis of NSCLC both in vitro and in vivo.Mechanically,circTLCD4-RWDD3 physically interacted with hnRNPA2B1 and mediated the SUMO2 modification at K108 residue of hnRNPA2B1 by upregulating UBC9.Subsequently,circTLCD4-RWDD3-induced SUMOylated hnRNPA2B1 was recognized by the SUMO interaction motif(SIM)of ALIX and activated ALIX to recruit ESCRT-III,thereby facilitating the sorting of circTLCD4-RWDD3 into NSCLC cell-derived EVs.Moreover,EV-packaged circTLCD4-RWDD3 was internalized by lymphatic endothelial cells to activate the transcription of PROX1,resulting in the lymphangiogenesis and LN metastasis of NSCLC.Importantly,blocking EV-mediated transmission of circTLCD4-RWDD3 via mutating SIM in ALIX or K108 residue of hnRNPA2B1 inhibited the lymphangiogenesis and LN metastasis of NSCLC in vivo.Our findings reveal a precise mechanism underlying SUMOylated hnRNPA2B1-induced EV packaging of circTLCD4-RWDD3 in facilitating LN metastasis of NSCLC,suggesting that EV-packaged circTLCD4-RWDD3 could be a potential therapeutic target against LN metastatic NSCLC. | Xiayao Diao Chao Guo Hanhao Zheng Ke Zhao Yuming Luo Mingjie An Yan Lin Jiancheng Chen Yuanlong Li Yuting Li Xuehan Gao Jiaqi Zhang Mengxin Zhou Wenliang Bai Lei Liu Guige Wang Lanjun Zhang Xiaotian He Rusi Zhang Zhihua Li Changhao Chen Shanqing Li | 2023 | Signal Transduction and Targeted Therapy2023，8，12： | 0 |
| 8 | Edible insects as a food source:a review显示文摘The practice of eating insects has been documented for a long time.With obvious economic and environmental advantages,edible insects are in the spotlight recently due to their enormous potential in multiple industries.A number of research has been conducted to maximize their value,but concerns have been raised for the food security.In this review,we compared the characteristics of edible insects with other traditional protein sources.The progress of modern entomophagy was introduced.The benefits and risks of eating insects were summarized.Additionally,the shortcoming of current production were discussed.Feasible ways and strategies were proposed to promote the consumption of edible insects. | Chufei Tang Ding Yang Huaijian Liao Hongwu Sun Chuanjing Liu Lanjun Wei Fanfan Li | 2019 | Food Production, Processing and Nutrition2019，1，1： | 0 |
| 9 | Influence of impact energy on work hardening ability of austenitic manganese steel and its mechanism显示文摘To further understand the hardening mechanism of austenitic manganese steel under actual working conditions, the work hardening ability was studied and the microstructures of austenitic manganese steel were observed under different impact energies. The work hardening mechanism was also analyzed. The results show that the best strain hardening effect could be received only when the impact energy reaches or exceeds the critical impact energy. The microstructural observations reveal that dislocations, stacking faults and twins increase with raising impact energy of the tested specimens. The hardening mechanism changes at different hardening degrees. It is mainly dislocation and slip hardening below the critical impact energy, but it changes to the twinning hardening mechanism when the impact energy is above the critical impact energy. | Li Xiaoyun Wu Wei Zu Fangqiu Liu Lanjun Zhang Xianfeng | 2012 | China Foundry2012，9，3： | 0 |
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