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| 1 | First-generation EGFR tyrosine kinase inhibitor therapy in 106 patients with compound EGFR-mutated lung cancer: a single institution’s clinical practice experience显示文摘Background:The antitumour efficacy of tyrosine kinase inhibitors(TKIs)in lung cancer patients with compound epidermal growth factor receptor(EGFR)mutations has not been resolved.Our study summarizes a single institutional experience of first-generation TKI therapy for lung cancers with compound EGFR mutations.Methods:A total of 106 consecutive patients with tumours bearing compound EGFR mutations were identified between January 2012 and May 2016;all patients received first-generation TKI therapy.Deletions in exon 19 and the L858R point mutation in exon 21 were considered common mutations;T790M was considered separately because of its association with TKIs resistances.Any other mutation was defined as a rare mutation.Patients were divided as follows:double common mutations(group A);common plus T790M mutations(group B);common plus rare muta-tions(group C);double rare mutations(group D);and rare plus T790M mutations(group E).A separate group of 115 consecutive patients with a single common mutation was created for comparative analysis(group F).Results:The frequency of patients with compound EGFR was 2.9%(114/3925)and their response rate to first-genera-tion TKIs was 50.9%,which was not significantly different from group F(67.0%,P=0.088).The progression-free survival(PFS)of the 106 patients receiving TKI therapy was worse than that of group F(median,9.1 vs.13.0 months,respec-tively;P<0.001).The PFS of the compound mutation group was shorter than that of the single common mutation group(median,10.1 months in group A,P=0.240;9.1 months in group B,P<0.001;9.6 months in group C,P=0.010;6.5 months in group D,P=0.048;5.4 months in group E,P=0.017).Patients with a co-occurring mutation in exon 20(excluding T790M)exhibited significantly worse PFS than the patients with other compound mutations or with a single common mutation(median,6.5 vs.9.1 vs.13.0 months,respectively,P=0.002).Conclusions:There was significant heterogeneity among the compound EGFR mutations and their response to first-generation TKIs.Individualized treatment in clinical practice should be considered for each case. | Xiangyang Yu Xuewen Zhang Zichen Zhang Yongbin Lin Yingsheng Wen Yongqiang Chen Weidong Wang Lanjun Zhang | 2018 | Cancer Communications2018，38，1： | 5 |
| 2 | 0.35%THz pulse conversion efficiency achieved by Ti:sapphire femtosecond laser filamentation in argon at 1 kHz repetition rate显示文摘In this study,an optical setup for generating terahertz(THz)pulses through a two-color femtosecond laser filament was carefully designed to achieve a precise overlap of two-color laser pulses in space and time.β-barium borate(BBO),α-BBO,and a dual-wavelength plate were used to compensate the phase delay of the two-color lasers.Tilting ofα-BBO could further realize the precise spatial overlap of the two beams by counteracting the walk-off effect.The maximum out-put THz pulse energy reached 21μJ in argon gas when using a commercial Ti:sapphire laser with a pulse energy of 6 mJ at a 1 kHz repetition rate.The corresponding conversion efficiency exceeded 0.35%. | Zhiqiang Yu Nan Zhang Jianxin Wang Zijie Dai Cheng Gong Lie Lin Lanjun Guo Weiwei Liu | 2022 | Opto-Electronic Advances2022，5，9： | 2 |
| 3 | Anti-Correlated Plasma and THz Pulse Generation during Two-Color Laser Filamentation in Air显示文摘The THz generation efficiency and the plasma density generated by a filament in air have been found anti-correlated when pumped by 800 nm+1600 nm two-color laser field.The plasma density near zero delay of two laser pulses has a minimum value,which is opposite to the trend of THz generation eficiency and contradicts common sense.The lower plasma density cannot be explained by the static tunneling model according to the conventional photocurrent model,but it might be attributed to the electron trapping by the excited states of nitrogen molecule.The present work also clarifies the dominant role of the drifting velocity accelerated by the two-color laser field during the THz pulse generation process.The results promote our understanding on the optimization of the THz generation efficiency by the two-color laser filamentation. | Zhiqiang Yu Lu Sun Nan Zhang Jianxin Wang Pengfei Qi Lanjun Guo Quan Sun Weiwei Liu Hiroaki Misawa | 2022 | Ultrafast Science2022，，6： | 2 |
| 4 | Dentification of differentially expressed genes between stomach tumor tissues and normal tissues using combination of SSH and Microarray hybridization显示文摘 | Wu Lanjun Mao Bingzhi Wang Shengqi | 2000 | International Conference on Engineering and Technological Sciences2000，，： | 1 |
| 5 | 第一代EGFR酪氨酸激酶抑制剂治疗106例复合EGFR突变肺癌患者:一项单中心临床研究显示文摘背景与目的酪氨酸激酶抑制剂(tyrosine kinase inhibitors,TKIs)对具有复合表皮生长因子受体(epidermal growth factor receptor,EGFR)突变的肺癌患者的疗效尚不确切。我们对第一代TKI治疗存在复合EGFR突变肺癌患者的疗效进行了单中心研究。方法2012年1月至2016年5月共确诊106例存在复合EGFR突变的连续肿瘤患者,所有患者均接受第一代TKI治疗。外显子19缺失和外显子21的L858R点突变为常见突变;由于T790M与TKIs耐药性相关,因此单独考虑T790M;其他突变均被定义为罕见突变。患者分组如下:双重常见突变(A组),常见加T790M突变(B组),常见加罕见突变(C组),双重罕见突变(D组)和罕见加T790M突变(E组)。我们建立了一个单独的组(F组),由115例存在单一常见突变的连续患者组成,以进行比较分析。结果EGFR复合突变患者的发生率为2.9%(114/3925),对第一代TKI的反应率为50.9%,与F组相比无显著性差异(67.0%,P=0.088)。接受TKI治疗的106例患者的无进展生存期(progression-free survival,PFS)比F组短(中位数,9.1个月vs.13.0个月,P<0.001)。复合突变组的PFS短于单一常见突变组(中位数,A组10.1个月,P=0.240;B组9.1个月,P<0.001;C组9.6个月,P=0.010;D组6.5个月,P=0.048;E组5.4个月,P=0.017)。外显子20中(不包括T790M)发生共同突变的患者显示出比其他复合突变或单一常见突变患者更短的PFS,差异具有统计学意义(中位数,6.5个月vs.9.1个月vs.13.0个月,P=0.002)。结论复合EGFR突变及其对第一代TKIs的反应存在显著的异质性。在临床中对每位患者都应考虑进行个体化治疗。 | Xiangyang Yu Xuewen Zhang Zichen Zhang Yongbin Lin Yingsheng Wen Yongqiang Chen Weidong Wang Lanjun Zhang | 2019 | 癌症2019，38，10： | 1 |
| 6 | Effect of sulfamethazine on the horizontal transfer of plasmid-mediated antibiotic resistance genes and its mechanism of action显示文摘As a new type of environmental pollutant,antibiotic resistance genes(ARGs)pose a huge challenge to global health.Horizontal gene transfer(HGT)represents an important route for the spread of ARGs.The widespread use of sulfamethazine(SM2)as a broad-spectrum bacteriostatic agent leads to high residual levels in the environment,thereby increasing the spread of ARGs.Therefore,we chose to study the effect of SM2 on the HGT of ARGs mediated by plasmid RP4 from Escherichia coli(E.coli)HB101 to E.coli NK5449 as well as its mechanism of action.The results showed that compared with the control group,SM2 at concentrations of 10 mg/L and 200 mg/L promoted the HGT of ARGs,but transfer frequency decreased at concentrations of 100 mg/L and 500 mg/L.The transfer frequency at 200 mg/L was 3.04×10^(−5),which was 1.34-fold of the control group.The mechanism of SM2 improving conjugation transfer is via enhancement of the mRNA expression of conjugation genes(trbBP,trfAP)and oxidative stress genes,inhibition of the mRNA expression of vertical transfer genes,up regulation of the outer membrane protein genes(ompC,ompA),promotion of the formation of cell pores,and improvement of the permeability of cell membrane to promote the conjugation transfer of plasmid RP4.The results of this study provide theoretical support for studying the spread of ARGs in the environment. | Xiaojing Yan Wenwen Liu Shengfang Wen Lanjun Wang Lusheng Zhu Jun Wang Young Mo Kim Jinhua Wang | 2023 | Journal of Environmental Sciences2023，，5： | 1 |
| 7 | 中国人非小细胞肺癌表皮生长因子受体EGFR基因突变与血浆纤维蛋白原水平之间的相关性(英文)显示文摘Objective:The plasma fibrinogen levels had not only been used as an independent prognostic parameter for the patients with non-small cell lung cancer (NSCLC), but also as a promising biomarker for evaluating the efficacy of chemotherapy. This study aimed to investigate the correlation between the plasma fibrinogen levels and epidermal growth factor receptor (EGFR) gene mutation and clinical-pathological characteristics of Chinese patients with NSCLC. Methods:In this retrospective study, NSCLC specimens collected from 352 patients between November 2009 and November 2011 were selected to detect EGFR gene mutation with real-time polymerase chain reaction (RT-PCR). In these specimens, 308 ones were also detected EGFR gene copy number with fluorescence in situ hybridization (FISH). Coagulation makers were examined prior to the operations. The association between the plasma fibrinogen levels and EGFR gene mutation and clinical-pathological characteristics were analyzed using SPSS 16.0 software. Results:The median pre-operation plasma fibrinogen level was 3.55 g/L (109/352) patients with higher plasma fibrinogen level (> 4.0 g/L). The lower plasma fibrinogen levels correlated significantly with EGFR gene mutations (P < 0.001), the similar result was seen in platelet counts (P = 0.026). A linear correlation was found between the plasma fibrinogen levels and the platelet counts in NSCLC patients (R 2 = 0.209, P < 0.001). Pre-operation plasma fibrinogen levels correlated with gender (P < 0.001), smoking status (P < 0.001), and histology (P < 0.001). There were significant link between the above clinical-pathological characteristics and EGFR gene mutations. In addition, EGFR gene mutation was correlated with FISH-positive status (P < 0.001). Moreover, both plasma fibrinogen level (P = 0.024) and the EGFR gene copy number (P = 0.040) had significant relationships with the pathological TNM stage. Conclusion:This study showed that a significant relevance between plasma fibrinogen levels and EGFR gene mutations. The plasma fibrinogen level might be as a clinical decision parameter for evaluating the efficacy of anti-EGFR tyrosine kinase inhibitors (TKIs) in NSCLC. The patients of NSCLC had higher indicate have poor benefits from anti-EFGR TKIs. In addition, pre-operation plasma fibrinogen level could be used as an indepedent prognostic biomarker for the patients with NSCLC. | Jianfei Zhu Ling Cai Haoxian Yang Yinsheng Wen Junye Wang Tiehua Rong Lanjun Zhang | 2013 | The Chinese-German Journal of Clinical Oncology2013，12，5： | 0 |
| 8 | Digital twins in smart farming:An autoware-based simulator for autonomous agricultural vehicles显示文摘Digital twins can improve the level of control over physical entities and help manage complex systems by integrating a range of technologies.The autonomous agricultural machine has shown revolutionary effects on labor reduction and utilization rate in field works.Autonomous vehicles in precision agriculture have the potential to improve competitiveness compared to current crop production methods and have become a research hotspot.However,the development time and resources required in experiments have limited the research in this area.Simulation tools in unmanned farming that are required to enable more efficient,reliable,and safe autonomy are increasingly demanding.Inspired by the recent development of an open-source virtual simulation platform,this study proposed an autoware-based simulator to evaluate the performance of agricultural machine guidance based on digital twins.Oblique photogrammetry using drones is used to construct threedimensional maps of fields at the same scale as reality.A communication format suitable for agricultural machines was developed for data input and output,along with an inter-node communication methodology.The conversion,publishing,and maintenance of multiple coordinate systems were completed based on ROS(Robot Operating System).Coverage path planning was performed using hybrid curves based on Bézier curves,and it was tested in both a simulation environment and actual fields with the aid of Pure Pursuit algorithms and PID controllers. | Xin Zhao Wanli Wang Long Wen Zhibo Chen Sixian Wu Kun Zhou Mengyao Sun Lanjun Xu Bingbing Hu Caicong Wu | 2023 | International Journal of Agricultural and Biological Engineering2023，16，4： | 0 |
| 9 | SUMOylation-triggered ALIX activation modulates extracellular vesicles circTLCD4-RWDD3 to promote lymphatic metastasis of non-small cell lung cancer显示文摘Lymph node(LN)metastasis is one of the predominant metastatic routes of non-small cell lung cancer(NSCLC)and is considered as a leading cause for the unsatisfactory prognosis of patients.Although lymphangiogenesis is well-recognized as a crucial process in mediating LN metastasis,the regulatory mechanism involving lymphangiogenesis and LN metastasis in NSCLC remains unclear.In this study,we employed high-throughput sequencing to identify a novel circular RNA(circRNA),circTLCD4-RWDD3,which was significantly upregulated in extracellular vesicles(EVs)from LN metastatic NSCLC and was positively associated with deteriorated OS and DFS of patients with NSCLC from multicenter clinical cohort.Downregulating the expression of EV-packaged circTLCD4-RWDD3 inhibited lymphangiogenesis and LN metastasis of NSCLC both in vitro and in vivo.Mechanically,circTLCD4-RWDD3 physically interacted with hnRNPA2B1 and mediated the SUMO2 modification at K108 residue of hnRNPA2B1 by upregulating UBC9.Subsequently,circTLCD4-RWDD3-induced SUMOylated hnRNPA2B1 was recognized by the SUMO interaction motif(SIM)of ALIX and activated ALIX to recruit ESCRT-III,thereby facilitating the sorting of circTLCD4-RWDD3 into NSCLC cell-derived EVs.Moreover,EV-packaged circTLCD4-RWDD3 was internalized by lymphatic endothelial cells to activate the transcription of PROX1,resulting in the lymphangiogenesis and LN metastasis of NSCLC.Importantly,blocking EV-mediated transmission of circTLCD4-RWDD3 via mutating SIM in ALIX or K108 residue of hnRNPA2B1 inhibited the lymphangiogenesis and LN metastasis of NSCLC in vivo.Our findings reveal a precise mechanism underlying SUMOylated hnRNPA2B1-induced EV packaging of circTLCD4-RWDD3 in facilitating LN metastasis of NSCLC,suggesting that EV-packaged circTLCD4-RWDD3 could be a potential therapeutic target against LN metastatic NSCLC. | Xiayao Diao Chao Guo Hanhao Zheng Ke Zhao Yuming Luo Mingjie An Yan Lin Jiancheng Chen Yuanlong Li Yuting Li Xuehan Gao Jiaqi Zhang Mengxin Zhou Wenliang Bai Lei Liu Guige Wang Lanjun Zhang Xiaotian He Rusi Zhang Zhihua Li Changhao Chen Shanqing Li | 2023 | Signal Transduction and Targeted Therapy2023，8，12： | 0 |
| 10 | A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides显示文摘Polysaccharides are important biomacromolecules existing in all plants,most of which are integrated into a fibrillar structure called the cell wall.In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility,our knowledge of cell wall architecture and function is greatly constrained.Here,we develop a single-molecule approach for identifying plant polysaccharides with acetylated modification levels.We designed a solid-state nanopore sensor supported by a free-standing SiNx membrane in fluidic cells.This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/mL and discriminate xylans with hyperacetylated and unacetylated modifications.We further demonstrated the capability of this method in distinguishing arabinoxylan and glucuronoxylan in monocot and dicot plants.Combining the data for categorizing polysaccharide mixtures,our study establishes a single-molecule platformfor polysaccharide analysis,opening a new avenue for understanding cell wall structures,and expanding polysaccharide applications. | Yao Cai Baocai Zhang Liyuan Liang Sen Wang Lanjun Zhang Liang Wang Hong-Liang Cui Yihua Zhou Deqiang Wang | 2021 | Plant Communications2021，2，2： | 0 |
| 11 | Microstructure and Mechanical Properties of Al-5Cu-4.5Mg-2.5Zn Squeeze Cast Alloy显示文摘Al-5Cu-4.5Mg-2.5Zn alloy was prepared and the alloy ingots were fabricated by squeeze casting in this work.Considering these negative effects of composition segregation and coarse second phases,some heat treatments were adopted in this research.Microstructures,element distribution,phase constitutions and mechanical properties of Al-5Cu-4.5Mg-2.5Zn alloy ingots before and after heat treatments were investigated.It was discovered that these heat treatments would influence and extremely optimize the microstructures and properties of Al-5Cu-4.5Mg-2.5Zn alloy.Except some residual S (Al_(2)CuMg) phase and a few of η phase,the precipitate free zone (PFZ) and the Guinier Preston zone (GPZ) formed in the alloy.It was also found that θ′′ (Al_(2)Cu) and η′′ (MgZn_(2)) phases formed and kept a consistent relationship with the aluminum matrix.As the result,these properties of ultimate tensile strength (UTS),percentage of elongation and Brinell hardness (HB) were greatly elevated.The UTS,percent of elongation and HB were 469 MPa,8.1% and 208 N/mm^(2),respectively. | 孙永根 WANG Yanchun SU Yanni SONG Xujie DU Lanjun CHENG Yuansheng 杜之明 | 2022 | Journal of Wuhan University of Technology(Materials Science)2022，37，5： | 0 |
| 12 | Diagnosing ΛHDE model with statefinder hierarchy and fractional growth parameter显示文摘Recently, a new dark energy model called ΛHDE was proposed. In this model, dark energy consists of two parts: cosmological constant Λ and holographic dark energy(HDE). Two key parameters of this model are the fractional density of cosmological constant ?_(Λ0), and the dimensionless HDE parameter c. Since these two parameters determine the dynamical properties of DE and the destiny of universe, it is important to study the impacts of different values of ?_(Λ0) and c on the ΛHDE model. In this paper,we apply various DE diagnostic tools to diagnose ΛHDE models with different values of ?_(Λ0) and c; these tools include statefinder hierarchy{S_3^(1), S_4^(1)}, fractional growth parameter ?, and composite null diagnostic(CND), which is a combination of{S_3^(1), S_4^(1)}and ?. We find that:(1) adopting different values of ?_(Λ0) only has quantitative impacts on the evolution of the ΛHDE model, while adopting different c has qualitative impacts;(2) compared with S_3^(1), S_4^(1) can give larger differences among the cosmic evolutions of the ΛHDE model associated with different ?_(Λ0) or different c;(3) compared with the case of using a single diagnostic, adopting a CND pair has much stronger ability to diagnose the ΛHDE model. | LanJun Zhou Shuang Wang | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，7： | 0 |
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