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| 1 | GS6, A Member of the GRAS Gene Family,Negatively Regulates Grain Size in Rice显示文摘Grain size is an important yield related trait in rice.Intensive arti cial selection for grain size during domestication is evidenced by the larger grains of most of today’s cultivars compared with their wild relatives.However,the molecular genetic control of rice grain size is still not well characterized.Here,we report the identi cation and cloning of Grain Size 6(GS6),which plays an important role in reducing grain size in rice.A premature stop at the t348 position in the coding sequence(CDS)of GS6 increased grain width and weight signi cantly.Alignment of the CDS regions of GS6 in 90 rice materials revealed three GS6alleles.Most japonica varieties(95%)harbor the Type I haplotype,and 62.9%of indica varieties harbor the Type II haplotype.Association analysis revealed that the Type I haplotype tends to increase the width and weight of grains more than either of the Type II or Type III haplotypes.Further investigation of genetic diversity and the evolutionary mechanisms of GS6showed that the GS6gene was strongly selected in japonica cultivars.In addition,a'ggc'repeat region identi ed in the region that encodes the GRAS domain of GS6 played an important historic role in the domestication of grain size in rice.Knowledge of the function of GS6might aid efforts to elucidate the molecular mechanisms that control grain development and evolution in rice plants,and could facilitate the genetic improvement of rice yield. | Lianjun Sun Xiaojiao Li Yongcai Fu Zuofeng Zhu Lubin Tan Fengxia Liu Xianyou Sun Xuewen Sun Chuanqing Sun | 2013 | Journal of Integrative Plant Biology2013，55，10： | 44 |
| 2 | Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages显示文摘An advanced backcross population of rice was used to identify the quantitative trait locus (QTL) controlling the cold-tolerance at booting to flowering stages. The recipient, Guichao 2 (GC2), was a commercial Indica rice; the donor Dongxiang common wild rice, was an accession of common wild rice (DXCWR, Oryza rufipogon Griff.). Three QTLs for cold-tolerance were detected on chromosomes 1, 6 and 11. Two of them coming from DXCWR could enhance the cold-tolerance of the backcross progenies. Moreover, one sterility QTL that could reduce the seed set rate of the backcross progenies by 78% was mapped on chromosome 5. | LIU Fengxia, SUN Chuanqing, TAN Lubin, FU Yongcai, LI Dejun & WANG Xiangkun Department of Plant Genetics and Breeding, China Agricultural University, National Key Laboratory of Agrobiology, Key Laboratory of Crop Genetic Improvement and Genome of the Ministry of Agriculture, Beijing 100094, China | 2003 | Chinese Science Bulletin2003，48，19： | 29 |
| 3 | Development of Oryza rufipogon and O. sativa Introgression Lines and Assessment for Yield-related Quantitative Trait Loci显示文摘基因渗入线人口有效地在印射量的特点 loci (QTL ) 被使用,识别有利基因,发现隐藏的遗传变异,评估处于多重条件的 QTL 的行动或相互作用并且提供为植物繁殖和基因研究的有利试验性的材料。在这研究,先进回交和连续 selfing 策略被用来开发基因渗入线(IL ) ,它源于 Oryza rufipogon Griff 的就职。从 Yuanjiang 县镇定,中国的云南省,著名计算机生产厂商他施主,和精英 indica 栽培变种 Teqing (O。sativa L.) ,接受者。从 O 的 Introgressionsegments。rufipogon 用 179 次多态的简单顺序重复(SSR ) 被屏蔽在每 IL 的染色体的标记。基因渗入线人口带的 Introgressed 片断包含了盖住整个 O 的 120 IL。rufipogon 染色体。同型结合的 O 的吝啬的数字。rufipogonsegments 每基因渗入线是大约 3.88。introgressed 片断的平均长度是近似的 25.5 厘米,并且有的大约 20.8% 这些片断缩放不到 10 厘米。eachIL 的染色体怀有 O 的 chromosomal 碎片。rufipogon 从 0.54% ~ 23.7%,与 5.79% 的全面一般水准。在每个地点, O 的替换的比率。rufipogon 等位基因有范围 of1.67-9.33,与 5.50 的一般水准。大量改变在词法并且 yield-relatedtraits 也在基因渗入线人口被发现。用单个点的分析,为产量和产量构成因素的 37 通常认为的 QTL 的一个总数与解释 phenotypic 的 7%-20% 在二个地点被检测。十九 QTL (51.4%) 在两个地点被检测,并且从在十五 loci (40.5%) 的 O.rufipogon 的等位基因在 Teging 背景改进了收益和产量构成因素。这些 O。rufipogon-O。sativa 基因渗入线将为从普通野米饭识别并且使用有利基因用作遗传物质…… | Lubin Tan | 2007 | Journal of Integrative Plant Biology2007，49，6： | 28 |
| 4 | Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon显示文摘土壤咸度是影响工厂生长和庄稼生产的主要不能生活的压力之一。在现在的学习,在米饭幼苗舞台的盐忍耐用 87 根基因渗入线(IL ) 被评估,它从在精英 indica 栽培变种 Teqing 和普通野米饭(Oryza rufipogon Griff ) 的就职之间的一个十字被导出。实质的变化为包括盐忍耐 20 的四个特点(圣) 被观察,相对的根干燥重量(RRW ) ,干燥重量(RSW ) 和相对总数弄干的相对射击重量(RTW ) 。圣断然显著地所有另外的三个特点地被相关。与这四个特点联系的 15 通常认为的量的特点 loci (QTL ) 的一个总数用单个点的分析被检测,它位于染色体 1, 2, 3, 6, 7, 9 和 10 与解释 phenotypic 变化的 8%26% 。O。在 13 QTL (86.7%) 的导出 rufipogon 的等位基因能在 Teqing 背景改进盐忍耐。影响 RRW, RSW 和 RTW 的四 QTL 簇在染色体上被发现 6, 7, 9 和 10 分别地。在这四 QTL 簇之中,包括三 QTL (qRRW10, qRSW10 和 qRTW10 ) 的主要的簇在染色体 10 的长手臂上在制造者 RM271 附近被发现,并且 O。分别地,当 phenotypic 变化由这解释为三个特点的三单个 QTL 从 19% ~ 26% 变化了时,在这三 loci 的导出 rufipogon 的等位基因与 22.7% , 17.3% 和 18.5% 的添加剂效果增加了 RRW, RSW 和 RTW。另外,几腌容忍的 IL 被选择并且能被用于识别并且利用有利的盐从普通野米饭的容忍的基因并且在盐使用容忍的米饭繁殖编程序。 | Lei Tian,Lubin Tan,Fengxia Liu,Hongwei Cai,Chuanqing Sun State Key Laboratory of Plant Physiology and Biochemistry,National Centre for Evaluation of Agricultural Wild Plant(Rice), Laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Department of Plant Genetics and Breeding,China Agricultural University,Beijing 100193,China | 2011 | Journal of Genetics and Genomics2011，38，12： | 26 |
| 5 | LHD1,an Allele of DTH8/Ghd8,Controls Late Heading Date in Common Wild Rice (Oryza rufipogon)显示文摘Flowering at suitable time is very important for plants to adapt to complicated environments and produce their seeds successfully for reproduction.In rice (Oryza rufipogon Griff.) photoperiod regulation is one of the important factors for controlling heading date.Common wild rice,the ancestor of cultivated rice,exhibits a late heading date and a more sensitive photoperiodic response than cultivated rice.Here,through map-based cloning,we identified a major quantitative trait loci (QTL) LHD1 (Late Heading Date 1),an allele of DTH8/Ghd8,which controls the late heading date of wild rice and encodes a putative HAP3/NF-YB/CBF-A subunit of the CCAAT-box-binding transcription factor.Sequence analysis revealed that several variants in the coding region of LHD1 were correlated with a late heading date,and a further complementary study successfully rescued the phenotype.These results suggest that a functional site for LHD1 could be among those variants present in the coding region.We also found that LHD1 could down-regulate the expression of several floral transition activators such as Ehd1,Hd3a and RFT1 under long-day conditions,but not under short-day conditions.This indicates that LHD1 may delay flowering by repressing the expression of Ehd1,Hd3a and RFT1 under long-day conditions. | Xiaodong Dai Younian Ding Lubin Tan Yongcai Fu Fengxia Liu Zuofeng Zhu Xianyou Sun Xuewen Sun Ping Gu Hongwei Cai Chuanqing Sun | 2012 | Journal of Integrative Plant Biology2012，54，10： | 23 |
| 6 | NARROW AND ROLLED LEAF 2 regulates leaf shape,male fertility, and seed size in rice显示文摘Grain yield in rice(Oryza sativa L.) is closely related to leaf and flower development. Coordinative regulation of leaf, pollen, and seed development in rice as a critical biological and agricultural question should be addressed. Here we identified two allelic rice mutants with narrow and semirolled leaves, named narrow and rolled leaf 2-1(nrl2-1) and nrl2-2. Map-based molecular cloning revealed that NRL2 encodes a novel protein with unknown biochemical function. The mutation of NRL2 caused pleiotropic effects, including a reduction in the number of longitudinal veins, defective abaxial sclerenchymatous cell differentiation, abnormal tapetum degeneration and microspore development, and the formation of more slender seeds compared with the wild type(WT). The NRL2 protein interacted with Rolling-leaf(RL14),causing the leaves of the nrl2 mutants to have a highercellulose content and lower lignin content than the WT, which may have been related to sclerenchymatous cell differentiation and tapetum degeneration. Thus, this gene is an essential developmental regulator controlling fundamental cellular and developmental processes, serving as a potential breeding target for high-yielding rice cultivars. | Shuangshuang Zhao Lei Zhao Fengxia Liu Yongzhen Wu Zuofeng Zhu Chuanqing Sun Lubin Tan | 2016 | Journal of Integrative Plant Biology2016，58，12： | 19 |
| 7 | Microarray-Assisted Fine-Mapping of Quantitative Trait Loci for Cold Tolerance in Rice显示文摘包括冷压力抵抗,许多重要农学的特点由量的特点 loci (QTL ) 复杂、控制。这些 QTL 的隔离将极大地有益于农业工业,但是它是一项挑战性的任务。这研究由把 microarray 与印射 QTL 相结合以便在早幼苗的舞台从寒冷容忍的变化 IL112 识别寒冷容忍的 QTL 探索了综合策略。IL112 的所有早幼苗在 45 点为 9 d 通常幸存吗?? | Fengxia Liu Wenying Xu Qian Song Lubin Tan Jiayong Liu Zuofeng zhu Yongcai Fu Zhen Su Chuanqing Sun | 2013 | Molecular Plant2013，6，3： | 17 |
| 8 | Natural Variations at TIG1 Encoding a TCP Tran scription Factor Contribute to Plant Architecture Domestication in Rice显示文摘The modification of plant architecture is a crucial target in rice domestication and modern genetic improvement.Although several genes regulating rice plant architecture have been characterized,the molecular mechanisms underlying rice plant architecture domestication remain largely unclear.Here we show that the inclined tiller growth in wild rice is controlled by a single dominant gene,TILLER INCLINED GROWTH 1 (T/Gf),which is located on chromosome 8 and encodes a TCP transcriptional activator.TIG1 is primarily expressed in the adaxial side of the tiller base,promotes cell elongation,and enlarges the tiller angle in wild rice.Variations in the TIG1 promoter of indica cultivars {tig1 allele) resulted in decreased expression of TIG1 in the adaxial side of tiller base and reduced cell length and tiller angle,leading to the transition from inclined tiller growth in wild rice to erect tiller growth during rice domestication.TIG1 positively regulates the expression of EXP A3,EXPB5,and SAUR39 to promote cell elongation and increase the tiller angle.Selective sweep analysis revealed that the tig1 allele was selected in indica cultivars by human beings.The cloning and characterization of TIG1 supports a new scenario of plant architecture evolution in rice. | Weifeng Zhang Lubin Tan Hongying Sun Xinhui Zhao Fengxia Liu Hongwei Cai Yongcai Fu Xianyou Sun Ping Gu Zuofeng Zhu Chuanqing Sun | 2019 | Molecular Plant2019，12，8： | 13 |
| 9 | Molecular Evolution of the TACl Gene from Rice(Oryza sativa L.)显示文摘Tiller 角度是栽培米饭(Oryza sativa ) 的建筑学的特色,自从它决定种密度和影响米饭产量。我们的以前的工作识别了 Tiller 角度控制 1 (TAC1 ) 作为一个主要量的特点地点,那控制米饭 tiller 角度。为了推进,澄清 TAC1 基因的进化描述,我们在 113 个栽培变化和野米饭的 48 就职之中比较了一个包含 TAC1 3164-bp genomic 区域,包括 O 的 43 就职。rufipogon 和 O 的五就职。nivara。一仅仅单个核苷酸多型性(SNP ) ,同义的替换,在编码栽培米饭变化的区域的 TAC1 被检测,而同义的和一 nonsynonymous SNP 在编码野米饭就职的区域的 TAC1 之中被检测。这些数据显示在编码区域的 TAC1 的很少自然变化和修正在栽培米饭和它的祖先以内发生在进化期间。在 O 的 TAC1 基因区域的核苷酸差异。sativa 和 O。0.00116 和 0.00112 的 rufipogon 分别地,进一步显示 TAC1 高度在米饭驯服的功课期间被保存了。TAC1 的功能的核苷酸多型性(FNP ) 仅仅在装饰用的梨树米饭组被发现。中性测试揭示了强壮的选择,特别在编码包含在装饰用的梨树米饭组的 FNP 的区域的 TAC1 的 3 胁腹区域。然而,没有选择发生在 indica 和野大米的组。从 TAC1 顺序分析导出的一棵种系发生的树建议 indica 和装饰用的梨树亚种在野米饭的驯服期间独立地产生了。 | Jiahuan Jiang Lubin Tan Zuofeng Zhu Yongcai Fu Fengxia Liu Hongwei Cai Chuanqing Sun | 2012 | Journal of Genetics and Genomics2012，39，10： | 12 |
| 10 | Clinical characteristics and effectiveness of antiviral agents in hospitalized children with infectious mononucleosis in China:A multicenter retrospective study显示文摘Importance:The clinical characteristics of infectious mononucleosis(IM)in Chinese children have not been evaluated in multicenter studies,and the effectiveness of antiviral treatment are controversial.Objective:To investigate the clinical characteristics of Chinese children with IM and current status of antiviral therapy for affected patients.Methods:Hospitalized patients with IM were enrolled between 2018 and 2020 in five children’s hospitals in China.The clinical characteristics were compared among four age groups:<3 years,3-<6 years,6-<10 years,and≥10 years.The clinical characteristics of IM and effectiveness of antiviral therapy were compared among patients receiving acyclovir(ACV),ganciclovir(GCV),and no antiviral therapy(i.e.,non-antiviral group).Results:In total,499 patients were analyzed;most patients were 3-<6 years of age.The most common symptoms and signs included fever(100%),lymphadenopathy(98.6%),pharyngitis(86.4%),eyelid edema(76.8%),and snoring(72.9%).There were significant differences in rash,hepatomegaly,and liver dysfunction among the four age groups.Patients aged<3 years had a lower incidence of liver dysfunction and a higher incidence of rash.Among the 499 patients,50.1%were treated with GCV,26.3%were treated with ACV,and 23.6%received no antiviral therapy.Compared with the non-antiviral group,patients in the ACV and GCV groups had longer durations of fever(P<0.001).There were no significant differences in the incidences of complications among the three treatment groups.Interpretation:The incidence of IM in Chinese children peaked at 3-<6 years of age.Clinical features of IM varied according to age.Patients receiving antiviral therapy exhibited more serious clinical manifestations than did patients without antiviral therapy.The effectiveness of antiviral therapy for IM requires further analysis. | Huili Hu Huiling Deng Jing Bi Yi Xu Shuangjie Li Yue Xie Xinrong Sun Dongmeng Wang Xufang Li Wenxian Ouyang Bing Hu Yufeng Zhang He Tang Chunxiao Fang Hui Zhang Lingyun Guo Chen Wang Tianyi Wang Fengxia Yang Tao Jiang Zhengde Xie Gang Liu | 2021 | Pediatric Investigation2021，5，3： | 12 |
| 11 | Recently duplicated sesterterpene(C25) gene clusters in Arabidopsis thaliana modulate root microbiota显示文摘Land plants co-speciate with a diversity of continually expanding plant specialized metabolites(PSMs) and root microbial communities(microbiota).Homeostatic interactions between plants and root microbiota are essential for plant survival in natural environments.A growing appreciation of microbiota for plant health is fuelling rapid advances in genetic mechanisms of controlling microbiota by host plants.PSMs have long been proposed to mediate plant and single microbe interactions.However,the effects of PSMs,especially those evolutionarily new PSMs,on root microbiota at community level remain to be elucidated.Here,we discovered sesterterpenes in Arabidopsis thaliana,produced by recently duplicated prenyltransferase-terpene synthase(PT-TPS) gene clusters,with neo-functionalization.A single-residue substitution played a critical role in the acquisition of sesterterpene synthase(sesterTPS) activity in Brassicaceae plants.Moreover,we found that the absence of two root-specific sesterterpenoids,with similar chemical structure,significantly affected root microbiota assembly in similar patterns.Our results not only demonstrate the sensitivity of plant microbiota to PSMs but also establish a complete framework of host plants to control root microbiota composition through evolutionarily dynamic PSMs. | Qingwen Chen Ting Jiang Yong-Xin Liu Haili Liu Tao Zhao Zhixi Liu Xiangchao Gan Asis Hallab Xuemei Wang Juan He Yihua Ma Fengxia Zhang Tao Jin M. Eric Schranz Yong Wang Yang Bai Guodong Wang | 2019 | Science China(Life Sciences)2019，62，7： | 11 |
| 12 | Successive inverse and normal magnetocaloric effects in the Mn-vacancy compound Mn0.95Co0.75Cu0.25Ge显示文摘Ferromagnetic-structural transformation has been studied widely in MnCoGe-based materials. However, the magnetostructural transition(MST) from antiferromagnetic(AFM) orthorhombic phase to ferromagnetic(FM) hexagonal phase, which may lead to a large inverse magnetocaloric effect(MCE), has rarely been reported. Here, the introduction of Mn vacancy lowers the structural transition temperature while retains the AFM state in the orthorhombic phase, thus successfully realizing the AFM-FM MST in Mn0.95Co0.75Cu0.25Ge. Moreover, successive inverse and normal MCEs are observed around the first-order AFM-FM MST and the second-order FM-paramagnetic(PM) transition, respectively. A thermostat is proposed based on this special feature, which could release heat above the critical temperature while absorb heat below the critical temperature by simply applying the same magnetization/demagnetization cycles. This thermostat can be very useful in many applications where a constant temperature is required, such as cryostats and incubators. | FengXia Liu Hu Zhang He Zhou DaoYong Cong RongJin Huang LiChen Wang Yi Long | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，7： | 9 |
| 13 | Characterization of the Formation of Branched Short-Chain Fatty Acid:CoAs for Bitter Acid Biosynthesis in Hop Glandular Trichomes显示文摘痛苦的酸,作为啤酒香料并且为他们的多样的生物活动为他们的使用知道,主要 formedin 跳跃(Humulus lupulus ) 腺的毛状体。分叉的短链的 acyl-CoAs (例如 isobutyryl-CoA, isovaleryl-CoA 和 2-methylbutyryl-CoA ) , derivedfrom 分支链氨基酸(BCAA ) 的降级,为痛苦的 acidsin 的生合成的必要积木跳跃。然而,很少关于部件被需要生产并且维持分叉的 short-chainacyl-CoAs 的水池在的被知道跳跃毛状体。这里,我们在场 CoA ligases 和 thioesterases 是在痛苦的酸生合成的 likelyinvolved 的证据的几根线。蛋白质有的 Recombinant HlCCL2 ( carboxyl CoA ligase )为象底层酸的 isovaleric 的高特定的活动( K 猫 /K m = 4100 s 1 M 1),而 recombinant HlCCL4 明确地利用了 isobutyric 酸( K 猫 /K m = 1800 s 1 M 1)并且 2-methylbutyric 酸( K 猫 /K m = 6900 s 1 M 1)作为底层。两 HlCCLs 跳跃 valerophenone synthase (HlVPS ) ,在腺的毛状体强烈被表示并且对细胞质局部性。有在酵母的 HlVPS 的 HlCCL2 和 HlCCL4 的合作表示导致了 acylphloroglucinols (为痛苦的酸生合成的直接先锋) 的重要生产, whichfurther 在 vivo 证实了这二 HlCCLs 的生物化学的功能。在线粒体催化 CCL 的反向的反应的 thioesterase 的功能的鉴定,和 thecomprehensive,基因的分析包含了 BCAA 分解代谢,支持了 cytosolic CoA ligases 在腺的毛状体为 linkingBCAA 降级和痛苦的酸生合成被要求的想法。进化和另外的可能的生理的 rolesof 分叉短链的丰满的酸: 在 planta 的 CoA ligases 也是 discussed.Key 词: | Haiyang Xu Fengxia Zhang Baoxiu Liu David V. Huhman Lloyd W. Sumner Richard A. Dixon Guodong Wang | 2013 | Molecular Plant2013，6，4： | 9 |
| 14 | Histological, hormonal and transcriptomic reveal the changes upon gibberellin-induced parthenocarpy in pear fruit显示文摘Phytohormones play crucial roles in fruit set regulation and development.Here,gibberellins(GA4+7),but not GA3,induced pear parthenocarpy.To systematically investigate the changes upon GA4+7 induced pear parthenocarpy,dynamic changes in histology,hormone and transcript levels were observed and identified in unpollinated,pollinated and GA4+7-treated ovaries.Mesocarp cells continued developing in both GA4+7-treated and pollinated ovaries.In unpollinated ovaries,mesocarp cells stopped developing 14 days after anthesis.During fruit set process,GA4+7,but not GA1+3,increased after pollination.Abscisic acid(ABA)accumulation was significantly repressed by GA4+7 or pollination,but under unpollinated conditions,ABA was produced in large quantities.Moreover,indole-3-acetic acid biosynthesis was not induced by GA4+7 or pollination treatments.Details of this GA–auxin–ABA cross-linked gene network were determined by a comparative transcriptome analysis.The indole-3-acetic acid transport-related genes,mainly auxin efflux carrier component genes,were induced in both GA4+7-treated and pollinated ovaries.ABA biosynthetic genes of the 9-cis-epoxycarotenoid dioxygenase family were repressed by GA4+7 and pollination.Moreover,directly related genes in the downstream parthenocarpy network involved in cell division and expansion(upregulated),and MADS-box family genes(downregulated),were also identified.Thus,a model of GA-induced hormonal balance and its effects on parthenocarpy were established. | Lulu Liu Zhigang Wang Jianlong Liu Fengxia Liu Rui Zhai Chunqin Zhu Huibin Wang Fengwang Ma Lingfei Xu | 2018 | Horticulture Research2018，5，1： | 7 |
| 15 | Ferroptosis as a novel form of regulated cell death:Implications in the pathogenesis,oncometabolism and treatment of human cancer显示文摘The treatment of cancer mainly involves surgical excision supplemented by radiotherapy and chemotherapy.Chemotherapy drugs act by interfering with tumor growth and inducing the death of cancer cells.Anti-tumor drugs were developed to induce apoptosis,but some patient’s show apoptosis escape and chemotherapy resistance.Therefore,other forms of cell death that can overcome the resistance of tumor cells are important in the context of cancer treatment.Ferroptosis is a newly discovered iron-dependent,non-apoptotic type of cell death that is highly negatively correlated with cancer development.Ferroptosis is mainly caused by the abnormal increase in iron-dependent lipid reactive oxygen species and the imbalance of redox homeostasis.This review summarizes the progression and regulatory mechanism of ferroptosis in cancer and discusses its possible clinical applications in cancer diagnosis and treatment. | Feifei Pu Fengxia Chen Zhicai Zhang Deyao Shi Binlong Zhong Xiao Lv Andrew Blake Tucker Jiaming Fan Alexander J.Li Kevin Qin Daniel Hu Connie Chen Hao Wang Fang He Na Ni Linjuan Huang Qing Liu William Wagstaff Hue H.Luu Rex C.Haydon Le Shen Tong-Chuan He Jianxiang Liu Zengwu Shao | 2022 | Genes & Diseases2022，9，2： | 6 |
| 16 | An analytical biomarker for treatment of patients with recurrent B-ALL after remission induced by infusion of anti-CD19 chimeric antigen receptor T(CAR-T) cells显示文摘Anti-CD19 chimeric antigen receptor-modified T(CAR-T-19) cells have emerged as a powerful targeted immunotherapy for B-cell lineage acute lymphoblastic leukemia with a remarkable clinical response in recent trials. Nonetheless, few data are available on the subsequent clinical monitoring and treatment of the patients, especially those with disease recurrence after CAR-T-19 cell infusion. Here, we analyzed three patients who survived after our phase I clinical trial and who were studied by means of biomarkers reflecting persistence of CAR-T-19 cells in vivo and predictive factors directing further treatment. One patient achieved 9-week sustained complete remission and subsequently received an allogeneic hematopoietic stem cell transplant. Another patient who showed relapse after 20 weeks without detectable leukemia in the cerebrospinal fluid after CAR-T-19 cell treatment was able to achieve a morphological remission under the influence of stand-alone low-dose chemotherapeutic agents. The third patient gradually developed extensive extramedullary involvement in tissues with scarce immune-cell infiltration during a long period of hematopoietic remission after CAR-T-19 cell therapy. Long-term and discontinuous increases in serum cytokines(mainly interleukin 6 and C-reactive protein) were identified in two patients(Nos. 1 and 6) even though only a low copy number of CAR molecules could be detected in their peripheral blood. This finding was suggestive of persistent functional activity of CAR-T-19 cells. Combined analyses of laboratory biomarkers with their clinical manifestations before and after salvage treatment showed that the persistent immunosurveillance mediated by CAR-T-19 cells would inevitably potentiate the leukemia-killing effectiveness of subsequent chemotherapy in patients who showed relapse after CAR-T-19-induced remission. | Yajing Zhang Wenying Zhang Hanren Dai Yao Wang Fengxia Shi Chunmeng Wang Yelei Guo Yang Liu Meixia Chen Kaichao Feng Yan Zhang Chuanjie Liu Qingming Yang Suxia Li Weidong Han | 2016 | Science China(Life Sciences)2016，59，4： | 6 |
| 17 | Estrogen and insulin synergistically promote endometrial cancer progression via crosstalk between their receptor signaling pathways显示文摘Objective: Despite evidence that estrogens and insulin are involved in the development and progression of many cancers, their synergistic role in endometrial carcinoma(EC) has not been analyzed yet.Methods: Here, we investigated how estrogens act synergistically with insulin to promote EC progression. Cell growth in vitro and in vivo, effects of estradiol and insulin on apoptosis and cell cycle distribution, and expression and activation of estrogen receptor(ER), insulin receptor(InsR), and key proteins in the PI3K and MAPK pathways were examined after combined stimulation with estradiol and insulin.Results: Compared to EC cells treated with estradiol or insulin alone, those treated with both estradiol and insulin exhibited stronger stimulation. Estradiol significantly induced phosphorylation of InsR-β and IRS-1, whereas insulin significantly induced phosphorylation of ER-α. In addition, treatment with both insulin and estradiol together significantly increased the expression and phosphorylation of Akt, MAPK, and ERK. Notably, InsR-β inhibition had a limited effect on estradiol-dependent proliferation,cell cycle, and apoptosis, whereas ER-α inhibition had a limited insulin-dependent effect, in EC cell lines. Insulin and estradiol individually and synergistically promoted EC xenograft growth in mice.Conclusions: Estrogen and insulin play synergistic roles in EC carcinogenesis and progression by activating InsR-β and ER-α,promoting a crosstalk between them, and thereby resulting in the activation of downstream PI3K/Akt and MAPK/ERK signaling pathways. | Wenyan Tian Fei Teng Jinping Gao Chao Gao Guoyan Liu Yanfang Zhang Shizhu Yu Wei Zhang Yingmei Wang Fengxia Xue | 2019 | Cancer Biology & Medicine2019，16，1： | 6 |
| 18 | miR-506: a regulator of chemo-sensitivity through suppression of the RAD51-homologous recombination axis显示文摘Ovarian carcinoma is the most lethal gynecologic malignancy. Resistance to platinum is considered the major problem afecting prognosis. Our recent study established that micro RNA-506(mi R-506) expression was closely associated with progression-free survival and overall survival in two independent patient cohorts totaling 598 epithelial ovarian cancer cases. Further functional study demonstrated that mi R-506 could augment the response to cisplatin and olaparib through targeting RAD51 and suppressing homologous recombination in a panel of ovarian cancer cell lines. Systemic delivery of mi R-506 in an orthotopic ovarian cancer mouse model signiicantly augmented the cisplatin response, thus recapitulating the clinical observation. Therefore, mi R-506 plays a functionally important role in homologous recombination and has important therapeutic value for sensitizing cancer cells to chemotherapy, especially in chemo-resistant patients with attenuated expression of mi R-506. | Guoyan Liu Fengxia Xue Wei Zhang | 2015 | Chinese Journal of Cancer2015，34，11： | 4 |
| 19 | Relationship between Lightning Activity and Tropospheric Nitrogen Dioxide and the Estimation of Lightning-produced Nitrogen Oxides over China显示文摘To better understand the relationship between lightning activity and nitrogen oxides(NOX) in the troposphere and to estimate lightning-produced NOX(LNOX) production in China more precisely, spatial and temporal distributions of vertical column densities of tropospheric nitrogen dioxide(NO_2VCDs) and lightning activity were analyzed using satellite measurements. The results showed that the spatial distribution of lightning activity is greater in the east than in the west of China, as with NO_2 VCDs. However, the seasonal and annual variation between lightning and NO_2 density show different trends in the east and west. The central Tibetan Plateau is sparsely populated without modern industry, and NO_2 VCDs across the plateau are barely affected by anthropogenic sources. The plateau is an ideal area to study LNOX. By analyzing 15 years of satellite data from that region, it was found that lightning density is in strong agreement with annual, spatial and seasonal variations of NO_2 VCDs, with a correlation coefficient of 0.79 from the linear fit. Combining Beirle's method and the linear fit equation,LNOXproduction in the Chinese interior was determined to be 0.07(0.02–0.27) Tg N yr^(-1) for 1997–2012, within the range of 0.016–0.384 Tg N yr^(-1) from previous estimates. | Fengxia GUO Xiaoyu JU Min BAO Ganyi LU Zupei LIU Yawen LI Yijun MU | 2017 | Advances in Atmospheric Sciences2017，34，2： | 4 |
| 20 | Effects of IBA on the rooting of branch cuttings of Chinese jujube (Zizyphus jujuba Mill.) and changes to nutrients and endogenous hormones显示文摘‘Zhongqiusucui’ jujube secondary shoots were treated with 3-indolebutyric acid(IBA) at three concentrations, 500, 1000 and 1500 mg/L. Results show that IBA could significantly enhance rooting and root characteristics of cuttings and were best with IBA at 1500 mg/L. In the rooting process, the formation of adventitious roots was related to the consumption and accumulation of nutrients(soluble sugars and proteins) and the changes in endogenous hormones in phloem, leaf tips and leaf bases. The rooting of cuttings had a positive correlation with the consumption of soluble sugars during the period of callus formation and with the accumulation of soluble sugars during adventitious root formation and growth. Rooting was positively related to the breakdown of soluble proteins in the phloem when the callus formed, and had a positive correlation with its accumulation during adventitious root formation and growth. Leaf tips and leaf bases showed a reverse trend in changes of soluble protein. However, together with the phloem, leaf tips and leaf bases regulated and controlled the formation and development of adventitious roots. The main activities of soluble proteins exist in the leaf tips as this was the main source of soluble proteins. The relation between rooting and IAA(indole-3-acetic acid) content in phloem was positive and thus a high concentration of IAA could benefit the induction and formation of adventitious roots. However, rooting was negatively related with ABA(abscisic acid) and GA(gibberellic acid) and a high concentration of both could inhibit the induction and formation of adventitious roots. Rooting had a positive correlation with phloem IAA/ABA ratios, and higher ratios could improve rooting. Low concentrations of ZR(zeatin riboside) triggered the induction of adventitious roots, while higher concentrations promoted root growth.Endogenous hormones in leaf tips and bases had an impact on rooting. The activities of endogenous hormones mainly existed in leaf tips because they play a major role in the production and consumption of IAA and its ABA content increased during rooting. The ZR in leaf tips influenced the rooting of cuttings, especially in the callus formation and rooting stage. Leaf tips were the main source of GA. | Fengxia Shao Sen Wang Wen Huang Zhiming Liu | 2018 | Journal of Forestry Research2018，29，6： | 4 |
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