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| 1 | Targeted Mutagenesis of NAC Transcription Factor Gene,OsN AC041,Leading to Salt Sensitivity in Rice显示文摘Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC(NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes(DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotictype ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding. | WANG Bo ZHONG Zhaohui ZHANG Huanhuan WANG Xia LIU Binglin YANG Lijia HAN Xiangyan YU Deshui ZHENG Xuelian WANG Chunguo SONG Wenqin CHEN Chengbin ZHANG Yong | 2019 | Rice science2019，26，2： | 8 |
| 2 | Multi-threshold second-order phase transition in laser显示文摘We present a theory of the multi-threshold second-order phase transition,and experimentally demonstrate the multi-threshold secondorder phase transition phenomenon.With carefully selected parameters,in an external cavity diode laser system,we observe secondorder phase transition with multiple(three or four) thresholds in the measured power-current-temperature three dimensional phase diagram.Such controlled death and revival of second-order phase transition sheds new insight into the nature of ubiquitous secondorder phase transition.Our theory and experiment show that the single threshold second-order phase transition is only a special case of the more general multi-threshold second-order phase transition,which is an even richer phenomenon. | ZHUANG Wei YU DeShui LIU ZhiWen CHEN JingBiao | 2011 | Chinese Science Bulletin2011，56，35： | 4 |
| 3 | Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma显示文摘我们以前在 hepatocellular 癌(HCC ) 识别了体的拷贝数字改变(CNA ) 的 1 241 个区域。在现在的学习,我们发现那新奇周期性的焦点的 amplicon, 1q24.1-24.2,在 HCC 指向 MPZL1 基因。尤其是,在 MPZL1 和 HCC 标本的 intrahepatic 转移的表示层次之间有积极关联。MPZL1 能显著地提高 HCC 房间的迁移、变形的潜力。而且,我们发现 MPZL1 由支持 HCC 房间移植的机制之一由导致支持 metastatic 蛋白质的 phosphorylation 和激活, cortactin。另外,我们发现 Src kinase 调停 cortactin 的 phosphorylation 和激活由 MPZL1 overexpression 导致了。一起拿,这些调查结果建议 MPZL1 是拷贝数字扩大的一个周期性的区域在 HCC 目标为 1q24.1-24.2 的新奇支持 metastatic 基因。 | Deshui Jia Ying Jing Zhenfeng Zhang Li Liu Jie Ding Fangyu Zhao Chao Ge Qifeng Wang Taoyang Chen Ming Yao Jinjun Li Jianren Gu Xianghuo He | 2014 | Cell Research2014，24，2： | 2 |
| 4 | An in situ study of inorganic nitrogen flow under different fertilization treatments on a wheat–maize rotation system surrounding Nansi Lake, China显示文摘 | Deshui Tan Lihua Jiang Shuying Tan Fuli Zheng Yu Xu Rongzong Cui Mei Wang Jing Shi Guosheng Li Zhaohui Liu | 2013 | Agricultural Water Management2013，，： | 1 |
| 5 | Application of Matlab to write a script for HFSS’s eigenmode solution of circular cavity显示文摘 | Ji Aiguo Liu Deshui | 2013 | Applied Mechanics and Materials2013，，： | 1 |
| 6 | Effects of Soil Improver on Wheat in Saline-Alkali Lands in the Yellow River Delta显示文摘Field experiment carried out to test the effects of soil improver on wheat yield and soil physical-chemical properties. The results indicated that soil improver could optimize soil aggregates structure, decrease soil bulk density, soil pH and soil salt content, increase soil organic matter and 1 000-grain weight, thereby enhancing wheat yield. With the increase of soil improver application amount, soil physical-chemical properties became better and wheat yield increased. However, there was no significant difference in the treatments with the application amounts of 3%, 4% and 5%. In addition, the treatment of reducing nitrogen showed no superiority in soil physical-chemical properties and wheat yield, indicating that sufficient nitrogen was essential for the growth of wheat. | Liang DONG Shenzhong TIAN Zeqiang SUN Xuejun WANG Zhaohui LIU Ruiqin LI Ye TIAN Deshui TAN Jiafa LUO | 2019 | Agricultural Biotechnology2019，8，2： | 0 |
| 7 | Optimization of Protective Agent Formulation for Soybean Rhizobium Strain HW-05 by Response Surface Methodology显示文摘[Objective] T his study aimed to investigate the protective effects of proteins,salts,sugars and trace elements on soybean rhizobium strain HW-05 by response surface methodology. [Method]Different types,combinations,ratios and concentrations of protective agents were designed. Under simulated conditions,the optimal protective agent formulation was screened for improving the survival rate and survival time of soybean rhizobium strain HW-05. The optimal combination of significant factors was determined by Box-Behnken central composite design. [Result] T hree significant factors affecting effective number of viable cells were screened,including peptone,xanthan gum and Na Cl. The final concentration of each compound was optimized: 0. 13% peptone,0. 011% xanthan gum,0. 30%Na Cl. [Conclusion] After addition of protective agent and preservation at room temperature for six months,effective number of viable cells of soybean rhizobium strain HW-05 reached 3.185 × 10~8 CFU / ml. The survival rate of HW-05 cells was improved by more than 25% compared with the control group(2.458×10~8 CFU / ml). | Tao ZHEN Wei CHEN Changqing SHA Xiancheng ZHANG Xinjie LIU Deshui YU | 2016 | Agricultural Biotechnology2016，5，4： | 0 |
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