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| 1 | Upregulation of vimentin and aberrant expression of E-cadherin /beta-catenin complex in oral squamous cell carcinomas:correlation with the clinicopathological features and patient outcome显示文摘Oral squamous cell carcinoma is a challenging oncology problem.A reliable biomarker for metastasis or high-risk prognosis in oral cancer patients remains undefined.Using quantitative immunohistochemistry,we examined the expression of vimentin,E-cadherin,and beta-catenin in 83 oral squamous cell carcinoma patients,and the relationships between the expression of these markers and specific clinicopathological features were analysed.The high expression of vimentin was observed in 23 of 43(53%) tumours from patients who eventually developed a recurrent tumour and was associated with recurrence and death(P < 0.001 and < 0.001,respectively).The decreased expression of E-cadherin was observed in 36 of 43(84%) tumours from patients who eventually developed a recurrent tumour and was also associated with recurrence and death(P < 0.001 and < 0.001,respectively).Although no correlation between beta-catenin expression in whole-tumour sections and clinicopathological features was observed,decreased beta-catenin expression at the tumour invasive front was closely associated with recurrence and death(P=0.002 and 0.002,respectively).The expression of vimentin and that of E-cadherin were associated with survival and were independent prognostic factors in univariate and multivariate analyses.Our data show that the overexpression of vimentin was closely associated with recurrence and death in oral squamous cell carcinoma patients.The combination of the upregulation of vimentin and aberrant expression of E-cadherin/beta-catenin complexes at the tumour invasive front may provide a useful prognostic marker in oral squamous cell carcinoma. | Liu,LK Jiang,XY Zhou,XX Wang,DM Song,XL Jiang,HB Nanjing Med Univ,Coll Stomatol,Dept Oral Pathol,Nanjing 210029,Jiangsu,Peoples R China Nanjing Med Univ,Inst Stomatol,Dept Oral Pathol,Nanjing 210029,Jiangsu,Peoples R China Nanjing Med Univ,Coll Stomatol,Dept Oral & Maxillofacial Surg,Nanjing 210029,Jiangsu,Peoples R China | 2010 | 南京医科大学学报(自然科学版)2010，30，5： | 25 |
| 2 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 3 | Protective effects of prostaglandin E1 on hepatocytes显示文摘INTRODUCTION E Numerous studies have demonstrated the protective action of prostaglandin E1(PGE1) on experimental animal models of liver injury and on patients with | Liu XL Fan DM | 2000 | World Journal of Gastroenterology2000，6，3： | 18 |
| 4 | Cloning and identification of an angiostatic molecule IP-10/crg-2显示文摘AIM To obtain human and murine cDNAsencoding IFN--y inducible protein 10 (lP--10) andcytokine responsive gene--2 (Crg-2).METHODS The encoding genes of lP-10 and Crg2 were amplified by RT--PCR from Cultured humanfibroblast cells and Balb/ c mouse liver treatedby IFN-y and TNF-a, respectively, and clonedinto plasmids of PUC19 and PGEM3Zf(+ ).RESULTS The nucleotide sequences ot theamplified DNA were confirmed by endonucleasesdigestion and sequencing.CONCLUSION Recombinant lP-10/ crg--2 geneclones with 306 hp and 314 hp inserts wereestablished for further research on biologicalactivities and ligands of hiP-10/mCrg--2. | Liu ZG Yang JH An HZ Wang HY He FT Han ZY Han Y Wu HP Xiao B Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 2 |
| 5 | Expression of mouse liver receptor homologue 1 in embryonic stem cells is directed by a novel promoter显示文摘 | Gao DM Wang LF Liu J Kong YY Wang Y Xie YH | 2006 | 中国生物学文摘2006，20，7： | 2 |
| 6 | A polymorphism in the protease-like domain of apolipoprotein (a) is associated with severe coronary artery disease 显示文摘 | Luke MM Kane JP Liu DM | 2007 | Arterioscler Thromb Vasc Biol2007，27，9： | 1 |
| 7 | Evolutionary patterns of hep- atitis B virus quasispecies under different selective pressures: correlation with antiviral efficacy显示文摘 | Liu F Chen L Yu DM | 2011 | Gut2011，60，9： | 1 |
| 8 | In-situ preparation of poly (prepylene fumarate)-hydroxyapatite composite显示文摘 | Hakimimehr D Liu DM Troczynski T | 2005 | Biomaterials2005，，： | 1 |
| 9 | In-vitro antitumor activity evaluation of hyperforin derivatives显示文摘 | Sun F Liu JY He F Liu Z Wang R Wang DM | 2011 | J Asian Nat Prod Res2011，13，8： | 1 |
| 10 | Effect of trehalose on PC12cells overexpressing wild-type or A53T mutantα-synuclein显示文摘 | Lan DM Liu FT Zhao J | 2012 | Neurochem Res2012，37，9： | 1 |
| 11 | Activation of persulfate by quinones: free radical reactions and implication for the degradation of PCBs显示文摘 | Fang GD Gao J Dionsiou DD Liu C Zhou DM | 2013 | Environment Science and Technology2013，47，： | 1 |
| 12 | ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance 显示文摘 | Brint EK Xu DM Liu HY | 2004 | Nature Immunology2004，5，： | 1 |
| 13 | Hepatitis B in China显示文摘 | Liu J Fan DM | 2007 | Lancet2007，369，9573： | 1 |
| 14 | AFP-spedfie CD4+ helper T-cell responses in healthy donors and HCC patients显示文摘 | Evdokimova VN Liu Y Potter DM | 2007 | J Immuno Therapy2007，30，4： | 1 |
| 15 | The safety and efficacy of a percutaneous closure device in patients undergoing uterine artery embollzation显示文摘 | Chrisman HB Liu DM Bui JT | 2005 | J Vasc Interv Radio12005，16，3： | 1 |
| 16 | Regimen-Specific Effects of RNA- Modified Chimeric Antigen Receptor T Cells in Mice with Advanced Leukemia显示文摘 | Barrett DM Liu X Jiang S | 2013 | Hum Gene Ther2013，24，8： | 1 |
| 17 | A multicenter study on the effect of continuous hemodiafiltration intensity on antibiotic pharma- cokinetics显示文摘 | Roberts DM Liu X Roberts JA | 2015 | Crit Care2015，19，1： | 1 |
| 18 | Effect of atorvastatin on plasma NT-proBNP and inflammatory cytokine expression in patients with heart failure显示文摘 | Duan HY Liu DM Qian P | 2015 | Genet Mol Res2015，14，15： | 1 |
| 19 | TNF-alpha receptor knockout mice are protected from the fibroproliferative effects of inhaled asbestos fibers显示文摘 | Liu JY Brass DM Hoyle GW | 1998 | Am J Pathol1998，153，6： | 1 |
| 20 | The immunogenicity and immunomoduclatory fucnction of osteogenic cells differentiated from mesenchymal stem cells显示文摘 | Liu H Kemeny DM Heng BC | 2006 | J Immuol2006，176，5： | 1 |
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