| 1 | Direct and single-step sensing of primary ovarian cancers related glycosidases显示文摘High sensitive,accurate detection for tumor-associated overexpressed enzyme activity is highly significant for further understanding enzyme function,discovering potential drugs,and early diagnosis and prevention of diseases.In this work,we developed a facile,direct and single-step detection platform for primary ovarian cancers related glycosidase activity based on the inner filter effect(IFE)between glycosidase catalytic product and black phosphorus quantum dots(BPQDs).Highly fluorescent BPQDs were successfully synthesized from bulk black phosphorus by a simple liquid exfoliation method.Under the catalysis ofβ-galactosidase,p-nitrophenyl-β-D-galactopyranoside(PNPG)was transformed into pnitrophenol(PNP)andβ-D-galactopyranoside.Meanwhile,the absorption of catalytic product PNP greatly overlapped with the excitation and emission spectra of fluorescent BPQDs,leading to the fluorescence quenching of BPQDs with a high quenching efficiency.The proposed sensing strategy provided a low detection limit of 0.76 U/L,which was 1-2 orders of magnitude lower than most unmodified sensing platforms.D-Galactal was selected as the inhibitor forβ-galactosidase to further assess the feasibility of screening potential inhibitors.The fluorescence recovery of BPQDs suggests that the unmodified sensing platform is feasible to discover potential drugs ofβ-galactosidase.Our work paves a general way in the detection of glycosidase activity with fluorescent BPQDs,which can be promising for glycosidase-related disease diagnosis and pathophysiology elucidation. | Dan Li Ling Liang Yawei Tang Linna Fu Shehua Xiao Quan Yuan | 2019 | Chinese Chemical Letters2019，30，5： | 2 |
| 3 | An asymmetric catalytic multi-component reaction enabled the green synthesis of isoserine derivatives and semi-synthesis of paclitaxel显示文摘Paclitaxel(Taxol^(@))as a cornerstone of chemotherapy remains in high demand.The semi-synthesis of paclitaxel via coupling of Taxus leaf-produced baccatin III and manually synthesized isoserine(C-13 side chain)is one of the solutions to avoid environmental damage caused by the sacrifice of the whole Taxus brevifolia.A green synthesis of the C-13 side chain is pivotal to the green production of paclitaxel.We herein report a green synthesis of isoserine derivatives and the semi-synthesis of paclitaxel via a straightforward assembly of isoserines based on an Rh2(TPA)3(OAc)/CPA1 co-catalyzed asymmetric multi-component reaction of ethyl diazoacetate,triethyl silanol and N-(anthrancen-9-ylmethyl)benzaldimine.The method is featured by improved atom economy,effective mass yield,and environmental factors compared to our previous racemic method and BMS company's semi-synthesis method.And the method allows for rapid access to paclitaxel derivatives with varied C-13 side chains. | Taoda Shi Xinglin Li Yukai Li Xiang Fu Linna Wu Dengqing Wu Wenhao Hu | 2023 | Green Synthesis and Catalysis2023，4，1： | 0 |
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