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| 1 | Testosterone attenuates pulmonary epithelial inflammation in male rats of COPD model through preventing NRF1-derived NF-κB signaling显示文摘Testosterone deficiency is common in male patients with chronic obstructive pulmonary disease (COPD) and may correlate with the deterioration of COPD. Clinical research suggests that testosterone replacement therapy may slow the COPD progression, but the specific biological pathway remains unclear. In this study, we explored the effect of testosterone on pulmonary inflammation in male COPD rats. The animals were co-treated with lipopolysaccharide (LPS) and cigarette to induce COPD. In COPD rats, nuclear respiratory factor 1 (NRF1) and NF-κB p65 were upregulated. In cigarette smoke extract (CSE)-, LPS-, or the combination of CSE and LPS-treated L132 cells, NRF1 and p65 were also upregulated. Silencing NRF1 resulted in the downregulation of p65. ChIP‒seq, ChIP‒qPCR, and luciferase results showed that NRF1 transcriptionally regulated p65. Both male and female COPD rats showed an upregulated NRF1 level and similar pulmonary morphology. But NRF1 was further upregulated in male castrated rats. Further supplementing testosterone in castrated male rats significantly reduced NRF1, pulmonary lesions, and inflammation. Supplementation of testosterone also reduced the phosphorylation of p65 and IKKβ induced by LPS or CSE in L132 cells. Our results suggest that testosterone plays a protective role in pulmonary epithelial inflammation of COPD through inhibition of NRF1-derived NF-κB signaling and the phosphorylation of p65. | Xueting Wang Linlin Huang Shan Jiang Kang Cheng Dan Wang Qianqian Luo Xiaomei Wu Li Zhu | 2021 | Journal of Molecular Cell Biology2021，13，2： | 4 |
| 2 | Inhibition of different types of inert dust on aluminum powder explosion显示文摘Aluminum powder explosion accidents occurred frequently,but the mechanism of aluminum powder explosion is unclear.Therefore,the inhibitive effect of aluminum powder explosion plays a key role.To evaluate the inhibition capacity of different kinds of carbonates and phosphates:Na H2PO4,(NH4)2HPO4,NH4H2PO4,KHCO3 and Na HCO3 on aluminum deflagrations,a standard 20-L spherical chamber was used to determine the explosion severity,characterized by the maximum explosion pressure(Pmax).New parameters have been proposed:the minimum significant inert concentration(MSIC)and the minimum complete inert concentration(MCIC),which characterized the effect of inert.Experimental results showed that from the minimum significant inert concentration(MSIC)and the minimum complete inert concentration(MCIC),phosphate can have a significant inhibiting effect.40%Na H2PO4 can totally inert the aluminum explosion,and 50%(NH4)2HPO4or 50%NH4H2PO4 can also suppress the explosion.Through simulation,phosphate mainly acts via a chemical inhibition pathway,which inhibits the reaction of aluminum powder and oxygen by catalyzing the recombination of H atoms and O atoms.Carbonate performs inhibition in chemically,producing CO2,diluting the oxygen around the aluminum powder.Studies indicated that the explosion pressure of the mixture decreases as the concentration of inert dust increases.However,when the concentration of carbonates was low,SEEP(suppressant enhanced explosion parameter)phenomenon was found.This research work has a potential industrial application in high hazard aluminum working condition,which can help decrease the explosion pressure and reduce the accident loss. | Linlin Dai Lin Hao Wei Kang Wei Xu Ning Shi HongyuanWei | 2020 | Chinese Journal of Chemical Engineering2020，28，7： | 1 |
| 3 | Over-expression of CD163, CD169, and CD151 is not sufficient to improve the susceptibility to porcine reproductive and respiratory syndrome virus infection in transgenic mice显示文摘Porcine reproductive and respiratory syndrome virus(PRRSV)is a major pathogen that causes reproductive failure and respiratory disease in pigs,resulting in devastating economic losses worldwide[1].Porcine alveolar macrophages(PAMs)are the primary target cells of PRRSV[2],and the putative receptors,including CD163,CD169,and CD151,play key roles during infection[3–6].However,the understanding of PRRSV infection and pathogenesis is | Zhengzhi Cui Shuaishuai Niu Jingjing Liu Lei Xu Yunping Dai Ning Li Youmin Kang Linlin Zhang Lei Zhou Shuyang Yu | 2017 | Science Bulletin2017，62，24： | 1 |
| 4 | Genome degradation promotes Salmonella pathoadaptation by remodeling fimbriae-mediated proinflammatory response显示文摘Understanding changes in pathogen behavior(e.g.increased virulence,a shift in transmission channel)is critical for the public health management of emerging infectious diseases.Genome degradation via gene depletion or inactivation is recognized as a pathoadaptive feature of the pathogen evolving with the host.However,little is known about the exact role of genome degradation in affecting pathogenic behavior,and the underlying molecular detail has yet to be examined.Using large-scale global avian-restricted Salmonella genomes spanning more than a century,we projected the genetic diversity of Salmonella Pullorum(bvSP)by showing increasingly antimicrobial-resistant ST92 prevalent in Chinese flocks.The phylogenomic analysis identified three lineages in bvSP,with an enhancement of virulence in the two recently emerged lineages(L2/L3),as evidenced in chicken and embryo infection assays.Notably,the ancestor L1 lineage resembles the Salmonella serovars with higher metabolic flexibilities and more robust environmental tolerance,indicating stepwise evolutionary trajectories towards avian-restricted lineages.Pan-genome analysis pinpointed fimbrial degradation from a virulent lineage.The later engineeredfim-deletion mutant,and all other five fimbrial systems,revealed behavior switching that restricted horizontal fecal-oral transmission but boosted virulence in chicks.By depleting fimbrial appendages,bvSP established persistent replication with less proinflammation in chick macrophages and adopted vertical transovarial transmission,accompanied by ever-increasing intensification in the poultry industry.Together,we uncovered a previously unseen paradigm for remodeling bacterial surface appendages that supplements virulence-enhanced evolution with increased vertical transmission. | Xiao Zhou Xiamei Kang Jiaqi Chen Yan Song Chenghao Jia Lin Teng Yanting Tang Zhijie Jiang Xianqi Peng Xiaoxi Tao Yiwei Xu Linlin Huang Xuebin Xu Yaohui Xu Tengfei Zhang Shenye Yu Jiansen Gong Shaohui Wang Yuqing Liu Guoqiang Zhu Corinna Kehrenberg Franois-Xavier Weill Paul Barrow Yan Li Guoping Zhao Min Yue | 2023 | National Science Review2023，10，10： | 0 |
| 5 | Self-shrinking supramolecular nanoparticles syndicate energy suppression and NIR-II mild photothermal amplification of mitochondrial oxidative stress for breast cancer therapy显示文摘Photothermal therapy(PTT)may lead to healthy tissue damage,tumor metastasis,and recurrence,which makes mild photothermal therapy(mild PTT)stand out.However,overcoming heat resistance,insufficient therapeutic effect,and poor photothermal conversion efficiency has become new challenge.Herein,we report a dynamic supramolecular nanocarrier formed from amide-sericin and aldehyde-polyhydroxy glucan(denoted as SDA),the loose cavity of which can be filled by using the pharmaceutical combination of lonidamine(LND)and NIR-II photothermal agent of IR-1061,producing SDLI with a tighter inner hole,smaller and uniform particle size and excellent stability due to multiple pulling forces.Moreover,the intricate internal network structure prevents the hydrophobic IR-1061 from forming aggregates in the small cavity,and the photothermal conversion efficiency(PCE)can reach 48.9%.At the acidic tumor microenvironment of pH 6.5,the controlled release of LND can solve the problem of heat resistance of NIR-II mild PTT and significantly improve the therapeutic effect of NIR-II mild PTT.Meanwhile,SDLI also shows a reasonable tumor inhibition rate,so the synergistic strategy of inhibiting tumor energy metabolism and NIR-II mild PTT to magnify mitochondrial oxidative stress,continuous cell stress state-induced immunogenic cell death to promote the induction of tumor apoptosis is proposed to achieve more effective cancer treatment. | Hongmei Liu Jiming Xu Mengjie Ye Hengbo Zhang Linlin Han Jingting Wang Peng Xue Yuejun Kang Zhigang Xu | 2024 | Nano Research2024，17，5： | 0 |
| 6 | A comparative study on the genomes,transcriptomes,and metabolic properties of Escherichia coli strains Nissle 1917,BL21(DE3),and MG1655显示文摘Escherichia coli is the most well-studied model prokaryote and has become an indispensable host for the biotech-nological production of proteins and biochemicals.In particular,the probiotic status of one E.coli strain,E.coli Nissle 1917(EcN)has helped it become a new favorite amongst synthetic biologists.To broaden its potential applications,here we assemble a comparative study on the genomes,transcriptomes,and metabolic properties of E.coli strains EcN,BL21(DE3),and MG1655.Comparative genomics data suggests that EcN possesses 1404 unique CDSs.In particular,EcN has additional iron transport systems which endow EcN with a higher tolerance to iron scarcity when compared to two other E.coli strains.EcN transcriptome data demonstrates that E.coli strains EcN,BL21(DE3),and MG1655 all have comparable activities of the central metabolic pathway,however only EcN inherits the arginine deiminase pathway.Additionally,we found that EcN displayed a lower expres-sion of ribosomal proteins compared to BL21(DE3)and MG1655.This comparative study on E.coli strains EcN,BL21(DE3),and MG1655 aims to provide a reference for further engineering EcN as a biotechnological tool. | Linlin Zhao Guobin Yin Yonglin Zhang Chaofan Duan Yang Wang Zhen Kang | 2022 | Engineering Microbiology2022，2，1： | 0 |
| 7 | Identification and Expression Analysis of Abscisic Acid Signal Transduction Genes in Hemp Seeds显示文摘Abscisic acid(ABA)is involved in regulating diverse biological processes,but its signal transduction genes and roles in hemp seed germination are not well known.Here,the ABA signaling pathway members,PYL,PP2C and SnRK2 gene families,were identified from the hemp reference genome,including 7 CsPYL(pyrab-actin resistance1-like,ABA receptor),8 CsPP2CA(group A protein phosphatase 2c),and 7 CsSnRK2(sucrose nonfermenting1-related protein kinase 2).The content of ABA in hemp seeds in germination stage is lower than that in non-germination stage.Exogenous ABA(1 or 10μM)treatment had a significant regulatory effect on the selected PYL,PP2C,SnRK2 gene families.CsAHG3 and CsHAI1 were most significantly affected by exogenous ABA treatment.Yeast two-hybrid experiments were performed to reveal that CsPYL5,CsSnRK2.2,and CsSnRK2.3 could interact with CsPP2CA7 and demonstrate that this interaction was ABA-independent.Our results indicated that CsPYL5,CsSnRK2.2,CsSnRK2.3 and CsPP2CA7 might involve in the ABA signaling transduction pathway of hemp seeds during the hemp seed germination stages.This study suggested that novel genetic views can be brought into investigation of ABA signaling pathway in hemp seeds and lay the foundation for further exploration of the mechanism of hemp seed germination. | Cong Hou Kang Ning Xiuye Wei Yufei Cheng Huatao Yu Haibin Yu Xia Liu Linlin Dong | 2023 | Phyton-International Journal of Experimental Botany2023，92，7： | 0 |
| 8 | Inactivated SARS-CoV-2 booster vaccine enhanced immune responses in patients with chronic liver diseases显示文摘Chronic liver disease(CLD)entails elevated risk of COVID-19 severity and mortality.The effectiveness of the booster dose of inactivated SARS-CoV-2 vaccine in stimulating antibody response in CLD patients is unclear.Therefore,we conducted a cross-sectional study involving 237 adult CLD patients and 170 healthy controls(HC)to analyze neutralizing antibodies(NAbs)against SARS-CoV-2 prototype and BA.4/5 variant,anti-receptor binding domain(RBD)IgG,and total anti-SARS-CoV-2 antibodies.Serum levels of the total anti-SARS-CoV-2 antibodies,anti-RBD IgG and inhibition efficacy of NAbs were significantly elevated in CLD patients after the booster dose compared with the pre-booster dose,but were relatively lower than those of HCs.Induced humoral responses decreased over time after booster vaccination.The neutralization efficiency of the serum against BA.4/5 increased but remained below the inhibition threshold.All four SARS-CoV-2 antibodies,including total anti-SARS-CoV-2 antibodies,anti-RBD IgG and NAbs against prototype and BA.4/5,were lower in patients with severe CLD than those with non-severe CLD.After booster shot,age and time after the last vaccine were the risk factors for seropositivity of NAb against BA.4/5 in CLD patients.Additionally,white blood cell counts and hepatitis B core antibodies were the protective factors,and severe liver disease was the risk factor associated with seropositivity of total anti-SARS-CoV-2 antibodies.Overall,our data uncovered that antibody responses were improved in CLD patients and peaked at 120 days after the booster vaccines.All antibodies excepting total anti-SARS-CoV-2 antibodies declined after peak.CLD patients exhibited impaired immunologic responses to vaccination and weakened NAbs against BA.4/5,which hindered the protective effect of the booster shot against Omicron prevalence.Cellular immune responses should be further evaluated to determine the optimal vaccine regimen for CLD patients. | Yongmei Liu Jianhua Lu Haoting Zhan Wenfang Yuan Xiaomeng Li Haiyan Kang Haolong Li Yongliang Chen Linlin Cheng Xingli Sun Haojie Zheng Wei Wang Erhei Dai Yongzhe Li | 2023 | Virologica Sinica2023，38，5： | 0 |
| 9 | A physics-based electromigration reliability model for interconnects lifetime prediction显示文摘Dear editor,When the technology node scales down to 14 nm, the backend-of-line(BEOL) in IC design faces the more serious challenges [1, 2]. The high-density integration induced by the technology innovation increases the number of interconnecting layers and reduces the metal pitch, making the placement and routing of BEOL more difficult. Meanwhile, due to the size effects, the maximum tolerable current density in narrow dimensions of metal lines can no longer meet the requirements of driving current density in interconnects. | Linlin CAI Wangyong CHEN Jinfeng KANG Gang DU Xiaoyan LIU Xing ZHANG | 2021 | Science China(Information Sciences)2021，64，11： | 0 |
| 10 | A minimum-domain impulse theory for unsteady aerodynamic force with discrete wake显示文摘We extend the impulse theory for unsteady aerodynamics, from its classic global form to finite-domain formulation, then to a minimum-domain version for discrete wake. Each extension has been confirmed numerically. The minimum-domain theory indicates that the numerical finding of Li and Lu(2012) is of general significance: The entire force is completely determined by only the time rate of impulse of those vortical structures still connecting to the body, along with the Lamb-vector integral thereof that captures the contribution of all the rest disconnected vortical structures. | Linlin Kang Luoqin Liu Weidong Su Jiezhi Wu | 2017 | Theoretical & Applied Mechanics Letters2017，7，5： | 0 |
| 11 | Selection and structural bases of potent broadly neutralizing antibodies from 3-dose vaccinees that are highly effective against diverse SARS-CoV-2 variants,including Omicron sublineages显示文摘Dear Editor,The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),belonging to the lineage B of the genus Betacoronavirus in the Coronaviridae family,is the causative agent of the C0VID19 pandemic,1 which has lasted for over two years,resulting in unprecedented public health and socioeconomic crisis.Several SARS-CoV-2 variants of interest(VOIs)as well as variants of concern(VOCs),including the Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.l),Delta(B.1.617.2)and the most recently identified Omicron sublineages(BA.1,BA.1.1,BA.2 and BA.3),have emerged at different time points over the course of the pandemic,leading to the resurge nee of outbreaks across the world.Among these variants. | Lei Wang Wangjun Fu Linlin Bao Zijing Jia Yuxia Zhang Yunjiao Zhou Wei Wu Jianbo wu Qianqian Zhang Yidan Gao Kang Wang Qiao Wang Chuan Qin Xiangxi Wang | 2022 | Cell Research2022，32，7： | 0 |
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