|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Analysis of genetic architecture and favorable allele usage of agronomic traits in a large collection of Chinese rice accessions显示文摘Genotyping and phenotyping large natural populations provide opportunities for population genomic analysis and genome-wide association studies(GWAS). Several rice populations have been re-sequenced in the past decade;however, many major Chinese rice cultivars were not included in these studies. Here, we report large-scale genomic and phenotypic datasets for a collection mainly comprised of 1,275 rice accessions of widely planted cultivars and parental hybrid rice lines from China. The population was divided into three indica/Xian and three japonica/Geng phylogenetic subgroups that correlate strongly with their geographic or breeding origins. We acquired a total of 146 phenotypic datasets for 29 agronomic traits under multi-environments for different subpopulations. With GWAS, we identified a total of 143 significant association loci, including three newly identified candidate genes or alleles that control heading date or amylose content. Our genotypic analysis of agronomically important genes in the population revealed that many favorable alleles are underused in elite accessions, suggesting they may be used to provide improvements in future breeding efforts. Our study provides useful resources for rice genetics research and breeding. | Xiuxiu Li Zhuo Chen Guomin Zhang Hongwei Lu Peng Qin Ming Qi Ying Yu Bingke Jiao Xianfeng Zhao Qiang Gao Hao Wang Yunyu Wu Juntao Ma Liyan Zhang Yongli Wang Lingwei Deng Shanguo Yao Zhukuang Cheng Diqiu Yu Lihuang Zhu Yongbiao Xue Chengcai Chu Aihong Li Shigui Li Chengzhi Liang | 2020 | Science China(Life Sciences)2020，63，11： | 8 |
| 2 | Structural characteristic and formation mechanism of hemihydrate calcium sulfate whiskers prepared using FGD gypsum显示文摘Hemihydrate calcium sulfate whiskers(HH-CSWs)were hydrothermally synthesized in a sulfuric acid solution at 120℃ for different holding times(20,40,and 60 min).The phase structures and morphologies were characterized by XRD and SEM,respectively.The XRD pattern of the sample under 60 min was refined via the Rietveld fitting method.The structure models of the HH-CSW sample under a 60-min holding time was established based on Rietveld fitting results.No difference in the positions of diffraction peaks was determined.The as-prepared holding time increased the intensity and aspect ratio of the diffraction peaks of both samples.In the prepared HH-CSW structure,Ca-O polyhedron is a 12-sided polyhedron similar to that in the gypsum structure;the Ca atom is located in two positions in the one-unit cell;the H_(2)O channel along the c-axis similar is O-shaped and bigger than that in hexagonal and monoclinic CaSO_(4)·0.5H_(2)O structures.Therefore,although the prepared HH-CSW crystals’structure are similar to that of the reported hexagonal and monoclinic CaSO_(4)·0.5H_(2)O structures,they are not the same.The formation mechanism of HH-CSW from flue gas desulfurization(FGD)gypsum is discussed based on the analysis of gypsum structure. | Xiaoting Zhang Lingwei Ran Xiao Wang Biao Jin Jianwu Zhang Shanying Li Liushuan Yang | 2022 | Particuology2022，20，3： | 7 |
| 3 | Effects of dry-wet cycles on three-dimensional pore structure and permeability characteristics of granite residual soil using X-ray micro computed tomography显示文摘Due to seasonal climate alterations,the microstructure and permeability of granite residual soil are easily affected by multiple dry-wet cycles.The X-ray micro computed tomography(micro-CT)acted as a nondestructive tool for characterizing the microstructure of soil samples exposed to a range of damage levels induced by dry-wet cycles.Subsequently,the variations of pore distribution and permeability due to drywet cycling effects were revealed based on three-dimensional(3D)pore distribution analysis and seepage simulations.According to the results,granite residual soils could be separated into four different components,namely,pores,clay,quartz,and hematite,from micro-CT images.The reconstructed 3D pore models dynamically demonstrated the expanding and connecting patterns of pore structures during drywet cycles.The values of porosity and connectivity are positively correlated with the number of dry-wet cycles,which were expressed by exponential and linear functions,respectively.The pore volume probability distribution curves of granite residual soil coincide with the χ^(2)distribution curve,which verifies the effectiveness of the assumption of χ^(2)distribution probability.The pore volume distribution curves suggest that the pores in soils were divided into four types based on their volumes,i.e.micropores,mesopores,macropores,and cracks.From a quantitative and visual perspective,considerable small pores are gradually transformed into cracks with a large volume and a high connectivity.Under the action of dry-wet cycles,the number of seepage flow streamlines which contribute to water permeation in seepage simulation increases distinctly,as well as the permeability and hydraulic conductivity.The calculated hydraulic conductivity is comparable with measured ones with an acceptable error margin in general,verifying the accuracy of seepage simulations based on micro-CT results. | Ran An Lingwei Kong Xianwei Zhang Chengsheng Li | 2022 | Journal of Rock Mechanics and Geotechnical Engineering2022，14，3： | 7 |
| 4 | Microstructure and Properties of ZL101 Alloy Affected by Substrate Movement Speed of a Novel Semisolid Continuous Micro Fused-Casting for Metal Process显示文摘A novel semisolid continuous Micro Fused-Casting additive manufacturing technology for producing a ZL101 alloy strip was developed, Micro Fused-Casting means that the semisolid metal slurry was pressed out from the outlet of bottom of crucible to the movable plate. The degree of sub-cooling was easily provided by movement of substrate in the micro fused-casting area. Under the aid of 3 D manufacturing software, the ZL101 alloy strip was solidified and formed layer by layer. The microstructure and properties of ZL101 semisolid slurry were improved by the cooling conditions. The results showed that the ZL101 alloy strip samples fabricated by Micro Fused-Casting had uniform structures and good performances with the substrate movement speed at 20 mm/s and the temperature at 590 ℃, the ultimate tensile strength and elongation of the ZL101 alloy strip reached 242.59 MPa and 7.71%, while the average Vickers hardness was 82.55 HV. | 罗晓强 韩永军 LI Qingbin HU Xiaoming XUE Lingwei | 2018 | Journal of Wuhan University of Technology(Materials Science)2018，33，3： | 6 |
| 5 | Enhanced magnetic properties in chemically inhomogeneous Nd-Dy-Fe-B sintered magnets by multi-main-phase process显示文摘Homogeneous substitution of Dy for Nd in the hard magnetic 2:14:1 phase can effectively enhance coercivity to ensure the high temperature operation,however,inevitably deteriorate remanence at expense.In this work,we performed a comparative investigation of the two magnets prepared by multimain-phase(co-sintering Nd_(2)Fe_(14)B and(Nd,Dy)_(2)Fe_(14)B powders)and single-main-phase(sintering(Nd,Dy)_(2)Fe_(14)B powders)approaches.The comparative investigation reveals that at the same Dy substitution level(2.16 wt%),such chemically inhomogeneous multi-main-phase magnet possesses better roomtemperature magnetic properties as well as thermal stability than those of the single-main-phase one with homogenous Dy distribution in the matrix grains.Room-temperature magnetic properties H_(Cj)=1664 kA/m,B_(r)=1.33 T and(BH)_(max)=350.4 kJ/m^(3)for the multi-main-phase magnet are all better than those for the single-main-phase magnet with H_(Cj)=1536 kA/m,B_(r)=1.29 T and(BH)_(max)=318.4 kj/m^(3).In addition,over the temperature range from 295 to 423 K,both the temperature coefficients of coercivity and remanence for the multi-main-phase magnet are also lower than that for the single-main-phase magnet.Such superior magnetic performance is attributed to the short-range magnetic interactions inside individual 2:14:1 phase grains and the long-range magnetostatic interactions between adjacent grains with inhomogeneous Dy distribution.Our work provides a feasible approach of enhancing coercivity and retaining energy product simultaneously in the Nd-Dy-Fe-B permanent magnets. | Xiaolian Liu Mengjie Pan Pei Zhang Tianyu Ma Lizhong Zhao Lingwei Li | 2021 | Journal of Rare Earths2021，39，5： | 5 |
| 6 | Numerical Simulation of Characteristics of CEX Ions in Ion Thruster Optical System显示文摘Charge exchange(CEX) ions could inflict severe damages on the ion thruster optical system.This article is aimed at investigating the characteristics of the CEX ions and their influences upon the optical system by means of particle-incell(PIC) ion simulation and Monte Carlo collision(MCC) methods.The results from numerical simulation indicate that despite the fact that CEX ions appear in the entire beamlet region near the ion optical system,the ones that present themselves downstream of the accelerator grid have good reason for attracting more attention.As their trajectories are significantly affected by the local electric field,a great number of CEX ions are accelerated toward grids resulting in sputtering erosion.When the influences of the CEX ions are considered in the numerical simulation,there could hardly be observed augments in the screen grid current,but the accelerator grid current increases from zero to 1.4% of the beamlet current.It can be understood from the numerical simulation that the CEX ions formed in the region far downstream of the accelerator grid should be blamed for the erosion on the downstream surface of the accelerator grid. | Zhong Lingwei Liu Yu Li Juan Gu Zuo Jiang Haocheng Wang Haixing Tang Haibin | 2010 | Chinese Journal of Aeronautics2010，23，1： | 5 |
| 7 | Amoxicillin effects on functional microbial community and spread of antibiotic resistance genes in amoxicillin manufacture wastewater treatment system显示文摘This study aimed to reveal how amoxicillin(AMX) affected the microbial community and the spread mechanism of antibiotic resistance genes(ARGs) in the AMX manufacture wastewater treatment system. For this purpose, a 1.47 L expanded granular sludge bed(EGSB) reactor was designed and run for 241 days treating artificial AMX manufacture wastewater. 454 pyrosequencing was applied to analyze functional microorganisms in the system. The antibiotic genes OXA_(-1), OXA_(-2), OXA_(-10), TEM_(-1), CTX-M_(-1), class I integrons(intI1) and 16 SrRNA genes were also examined in sludge samples. The results showed that the genera Ignavibacterium, Phocoenobacter,Spirochaeta, Aminobacterium and Cloacibacillus contributed to the degradation of different organic compounds(such as various sugars and amines). And the relative quantification of eachβ-lactam resistance gene in the study was changed with the increasing of AMX concentration.Furthermore the vertical gene transfer was the main driver for the spread of ARGs rather than horizontal transfer pathways in the system. | Lingwei Meng Xiangkun Li Xinran Wang Kaili Ma Gaige Liu Jie Zhang | 2017 | Journal of Environmental Sciences2017，29，11： | 5 |
| 8 | Characterization of four hemocyanin isoforms in Litopenaeus vannamei显示文摘In this study, the gene encoding hemocyanin subunit L, Lv Hc L, was cloned from Litopenaeus vannamei and the genomic organization was characterized. This gene was diverse with many SNPs and also had at least four isoforms, while one of them(Lv Hc L4) only had two exons and the exon2 was missed. Transcription analysis showed that these isoforms of Lv Hc L were up-regulated after WSSV challenge in WSSV-resistant shrimp, while the transcriptions were decreased constantly in WSSV-susceptible shrimp. It is suggested that the hemocyanin had rich polymorphism and was involved in the antiviral response. These results could extend our previous findings and provide insights into the immune feature of hemocyanin, which would be helpful for further studies aimed at antiviral mechanism in invertebrate. | XU Jingxiang RUAN Lingwei LI Zhen YU Xiaoman LI Sedong SHI Hong XU Xun | 2015 | Acta Oceanologica Sinica2015，34，2： | 4 |
| 9 | Achievement of promising cryogenic magnetocaloric performances in La_(1-x)Pr_(x)Fe_(12)B_(6)compounds显示文摘The magnetic refrigeration(MR)utilizing magnetocaloric effect(MCE)has been recognized as an environmentally friendly and energy efficiency technology.Here we presented the magnetic properties and MCE in Pr-doped La_(1-x)Pr_(x)Fe_(12)B_(6)(x=0.05-0.2)itinerant-electron metamagnetic(IEM)compounds.A small amount of Pr doping La site can greatly improve the peak values in the magnetic entropy change S_(M)(T)curves,especially under relatively low magnetic field changes(ΔH).Additionally,the peak temperature increases gradually and the magnetic hysteresis reduces gradually with increasing x.The observed MCE in present La_(1-x)Pr_(x)Fe_(12)B_(6)compounds is related to its field-induced first-ordered IEM transition.The peak values ofΔS_(M)for La_(1-x)Pr_(x)Fe_(12)B_(6)compounds reach 13.4,15.4,12.5 and 13.0 J/(kg K)at T_(C)~58,68,72and 89 K for x=0.05,0.10,0.15 and 0.2 under H of 0-7 T,respectively.The corresponding relative cooling power values are 462.3,480.7,372.4 and 375.7 J/kg.The present La_(1-x)Pr_(x)Fe_(12)B_(6)compounds could be good candidates for active MR application if the magnetic and thermal hysteresis can be further reduced.The present work indicates that the La Fe_(12)B_(6)-based material system could also exhibit promising magnetocaloric performances. | Zhipan Ma Xiaoshi Dong Zhenqian Zhang Lingwei Li | 2021 | Journal of Materials Science & Technology2021，，33： | 4 |
| 10 | Microbiologically influenced corrosion of 304 stainless steel by halophilic archaea Natronorubrum tibetense显示文摘The corrosion behavior of 304 stainless steel(SS)in the presence of aerobic halophilic archaea Natronorubrum tibetense was investigated.After 14 days of immersion,no obvious pitting pit was observed on the SS surface in the sterile medium.By contrast,the SS exhibited serious pitting corrosion with the largest pit depth of 5.0μm in the inoculated medium after 14 days.The results of electrochemical tests showed that the barrier property of the passive film decreased faster in the inoculated medium.The X-ray photoelectron spectroscopy results indicated that the detrimental Fe2+and Cr6+increased in the passive film under the influence of archaea N.tibetense,which resulted in the accelerated deterioration of passive film and promoted the pitting corrosion.Combined with the energy starvation tests,the microbiologically influenced corrosion mechanism of 304 SS caused by halophilic archaea N.tibetense was discussed finally. | Hongchang Qian Lingwei Ma Dawei Zhang Ziyu Li Luyao Huang Yuntian Lou Cuiwei Du | 2020 | Journal of Materials Science & Technology2020，42，11： | 3 |
| 11 | Evolution of cracks in the shear bands of granite residual soil显示文摘The evolution of shear bands and cracks plays an important role in landslides.However,there is no systematic method for classification of the cracks,which can be used to analyze the evolution of cracks in shear bands.In this study,X-ray computed tomography(CT)is used to observe the behavior of granite residual soil during a triaxial shear process.Based on the digital volume correlation(DVC)method,a crack classification method is established according to the connectivity characteristics of cracks before and after loading.Cracks are then divided into six classes:obsolete,brand-new,isolated,split,combined,and compound.With evolution of the shear bands,a large number of brand-new cracks accelerate the damages of materials at the mesoscale,resulting in a sharp decrease in strength.The volume of brandnew cracks increases rapidly with increasing axial strain,and their volume is greater than 50%when the strain reaches 12%,while the volume of compound cracks decreases from 54%to 21%.As cracks are the weakest areas in a material,brand-new cracks accelerate the development of shear bands.Finally,the coupling effect of shear bands and cracks destroys the soil strength. | Chengsheng Li Lingwei Kong Ran An | 2022 | Journal of Rock Mechanics and Geotechnical Engineering2022，14，6： | 3 |
| 12 | Magnetocaloric effect and slow magnetic relaxation behavior in binuclear rare earth based RE_(2)(L)_(2)(DMF)4(RE=Gd,Tb,and Dy) complexes显示文摘Three binuclear rare earth based complexes combining RE ions with semirigid tricarboxylic ligand(H_(3)L).namely,[RE_(2)(L)_(2)(DMF)_(4)][RE=Gd,Tb,and Dy;H_(3)L=5-((4-Carboxybenzyl)oxy)isophthalic acid;DMF=N,N-dimethylformamide]complexes,were fabricated success fully.The RE_(2)(L)_(2)(DMF)_(4) co mplexe s consist of two central RE ions with the same coordination environment which were connected by two tridentate bridging carboxylic groups and two syn-syn bidentate bridging carboxylic groups originating from the L^(3-)ligands to form the{RE_(2)}dimeric unit,and thus provides the basis for further constructing a dense three-dimensional(3 D)network structure.Moreover,the present RE_(2)(L)_(2)(DMF)_(4) complexes can be described by a topology diagram with the topology point symbol of{4^(2)·6}_(2){4^(4)·6^(2)·8^(7)·10^(2)}.Weak antiferromagnetic(AFM)coupling between the adjacent RE ions for all the present complexes was found according to the magnetic calculations.The observed significant cryogenic magnetocaloric effect(MCE)with the maximum magnetic entropy change-ΔS_(M)^(max) to be 26.3 J/(kg·K)withΔH=7 T in Gd_(2)(L)_(2)(DMF)_(4) complex makes it competitive for the cryogenic magnetic refrigerant.Moreover,the slow magnetic relaxation behavior at 0.2 T dc field with an obvious large U_(eff)/k=45(4)K and τ_(0)=6.5(2)×10^(-10)s was confirmed in Dy_(2)(L)_(2)(DMF)_(4)complex.This work not only provides an effective strategy for obtaining molecular materials with high MCE,but also confirms that tricarboxylate ligands are the ideal choice for constructing stable high dimensional geometric structures. | Zhenqian Zhang Yuwei Wu Haifeng Wang Lingwei Li | 2022 | Journal of Rare Earths2022，40，6： | 2 |
| 13 | CT radiomics can help screen the coronavirus disease2019(COVID-19): a preliminary study显示文摘The coronavirus disease 2019(COVID-19) is raging across the world.The radiomics,which explores huge amounts of features from medical image for disease diagnosis,may help the screen of the COVID-19.In this study,we aim to develop a radiomic signature to screen COVID-19 from CT images.We retrospectively collect 75 pneumonia patients from Beijing Youan Hospital,including 46 patients with COVID-19 and 29 other types of pneumonias.These patients are divided into training set(n = 50) and test set(n = 25) at random.We segment the lung lesions from the CT images,and extract 77 radiomic features from the lesions.Then unsupervised consensus clustering and multiple cross-validation are utilized to select the key features that are associated with the COVID-19.In the experiments,while twenty-three radiomic features are found to be highly associated with COVID-19,four key features are screened and used as the inputs of support vector machine to build the radiomic signature.We use area under the receiver operating characteristic curve(AUC) and calibration curve to assess the performance of our model.It yields AUCs of 0.862 and 0.826 in the training set and the test set respectively.We also perform the stratified analysis and find that its predictive ability is not affected by gender,age,chronic disease and degree of severity.In conclusion,we investigate the value of radiomics in screening COVID-19,and the experimental results suggest the radiomic signature could be a potential tool for diagnosis of COVID-19. | Mengjie FANG Bingxi HE Li LI Di DONG Xin YANG Cong LI Lingwei MENG Lianzhen ZHONG Hailin LI Hongjun LI Jie TIAN | 2020 | Science China(Information Sciences)2020，63，7： | 2 |
| 14 | Multi-action self-healing coatings with simultaneous recovery of corrosion resistance and adhesion strength显示文摘In this study, a new self-healing strategy that can simultaneously recover the corrosion resistance and the adhesion strength of coatings was introduced. The coating was based on a shape memory epoxy resin containing ethylene vinyl acetate(EVA) microspheres loaded with Ce(NO_(3))_(3)inhibitors, and was cured at a relatively high temperature to facilitate the fusion of adjacent microspheres for a strengthened self-healing effect. The electrochemical impedance spectroscopy(EIS) and scanning electrochemical microscopy(SECM) results demonstrated that the shape memory effect of epoxy matrix, the filling of molten EVA microspheres as well as the release of Ce(NO_(3))_(3)inhibitors contributed synergistically to suppress the corrosion reaction at the coating damage. After healing, the low frequency impedance modulus of the coatings containing Ce(NO_(3))_(3)-EVA microspheres was three orders of magnitude higher than that of the blank epoxy coating. The adhesion strength of the coatings containing Ce(NO_(3))_(3)-EVA microspheres on the metal substrate was also largely repaired thanks to the strong bonding effect of the EVA microspheres. | Chenhao Ren Yao Huang Wenkui Hao Dawei Zhang Xiejing Luo Lingwei Ma Jinke Wang Thee Chowwanonthapunya Chaofang Dong Xiaogang Li | 2022 | Journal of Materials Science & Technology2022，，6： | 2 |
| 15 | Comparison of ablative and compressive mechanical behavior of several PICA-like ablative materials显示文摘It is significant to compare the ablative and compressive mechanical behavior of different PICA-like materials in the engineering applications.The plasma wind tunnel ablation tests with high-entropy air and CO2 atmospheres,and compressive experiments in the ambient and 150℃,were conducted for three kinds of PICA-like materials(CF/PR-Si,CBCF/PR-SiOC and NQF/PR-Si composites).The traditional carbon/phenolic(C/PR)braided composites were also used for comparison.PICA-like materials have the better thermal insulation than traditional C/PR composite,especially for CBCF/PR-SiOC composite.The ablation behavior of CF/PR-Si and CBCF/PR-SiOC PICA-like materials in the CO2 atmosphere can produce a large amount of SiO2 in the form of coatings,oxide layers and skeletons on the ablated surface,which are greatly different from that in the air atmosphere.The compressive behavior of PICA-like material is greatly depended on the fiber fabrics,and exhibits the large discrete characteristics.The longer fiber in the PICA-like materials plays the role in maintaining the material integrity,while it may increase the thermal conductivity. | ZHANG Jun FANG GuoDong YANG LingWei LI Wei HONG ChangQing MENG SongHe | 2020 | Science China(Technological Sciences)2020，63，8： | 2 |
| 16 | Effect of furan π-bridge on the photovoltaic performance of D-A copolymers based on bi(alkylthio-thienyl)benzodithiophene and fluorobenzotriazole显示文摘The medium band gap donor-acceptor(D-A) copolymer J61 based on bi(alkylthio-thienyl)benzodithiophene as donor unit and fluorobenzotriazole as acceptor unit and thiophene as π-bridge has demonstrated excellent photovoltaic performance as donor material in nonfullerene polymer solar cells(PSCs) with narrow bandgap n-type organic semiconductor ITIC as acceptor.For studying the effect of π-bridges on the photovoltaic performance of the D-A copolymers,here we synthesized a new D-A copolymer J61-F based on the same donor and acceptor units as J61 but with furan π-bridges instead of thiophene.J61-F possesses a deeper the highest occupied molecular orbital(HOMO) level at-5.45 eV in comparison with that(-5.32 eV) of J61.The non-fullerene PSCs based on J61-F:ITIC exhibited a maximum power conversion efficiency(PCE) of 8.24%with a higher open-circuit voltage(V_(oc)) of 0.95 V,which is benefitted from the lower-lying HOMO energy level of J61-F donor material.The results indicate that main chain engineering by changing π-bridges is another effective way to tune the electronic energy levels of the conjugated D-A copolymers for the application as donor materials in non-fullerene PSCs. | Tinghai Yan Haijun Bin Yankang Yang Lingwei Xue Zhi-Guo Zhang Yongfang Li | 2017 | Science China Chemistry2017，60，4： | 1 |
| 17 | The Overseas Reception of Chinese Science Fiction Works from a Perspective of Western Interpretation Preference——A Case Study of Reader's Reception of Liu Cixin's The Three-Body Problem in the USA显示文摘The Three-Body Problem, written by Liu Cixin, won the 2015 Hugo Award for Best Novel, with over 200,000 copies in English already sold. This gure is hundreds of times the average sales volume of Chinese novels in the USA. Through qualitative and quantitative analyses of overseas readers' comments on The Three-Body Problem posted on the online platforms of Goodreads and Amazon, this paper concludes that the novel's success overseas lies in its catering to the concerns of overseas readers. The ThreeBody Problem is popular among overseas readership thanks to its re ection on the ultimate existence of mankind, science ction vision & imagination, literary familiarity & identi cation, ideological di erence-triggered cultural curiosity, and a good translation. This paper attempts to interpret Liu Cixin's work on the map of world literature from the perspective of overseas readers' preferences to provide references for contemporary Chinese science ction creation and overseas reception. | Liu Ge Li Yun Wu Lingwei | 2018 | Contemporary Social Sciences2018，3，6： | 1 |
| 18 | Ferromagnetic ordering in EuCu2P2显示文摘 | Dexuan Huo Jun Lin Guanghong Tang Lingwei Li Zhenghong Qian Toshiro Takabatake | 2011 | Journal of Physics: Conference Series2011，，1： | 1 |
| 19 | Erianin suppresses constitutive activation of MAPK signaling pathway by inhibition of CRAF and MEK1/2显示文摘Constitutive activation of RAS-RAF-MEK-ERK signaling pathway(MAPK pathway)frequently occurs in many cancers harboring RAS or RAF oncogenic mutations.Because of the paradoxical activation induced by a single use of BRAF or MEK inhibitors,dual-target RAF and MEK treatment is thought to be a promising strategy.In this work,we evaluated erianin is a novel inhibitor of CRAF and MEK1/2 kinases,thus suppressing constitutive activation of the MAPK signaling pathway induced by BRAF V600E or RAS mutations.KinaseProfiler enzyme profiling,surface plasmon resonance(SPR),isothermal titration calorimetry(ITC),cellular thermal shift assay,computational docking,and molecular dynamics simulations were utilized to screen and identify erianin binding to CRAF and MEK1/2.Kinase assay,luminescent ADP detection assay,and enzyme kinetics assay were investigated to identify the efficiency of erianin in CRAF and MEK1/2 kinase activity.Notably,erianin suppressed BRAF V600E or RAS mutant melanoma and colorectal cancer cell by inhibiting MEK1/2 and CRAF but not BRAF kinase activity.Moreover,erianin attenuated melanoma and colorectal cancer in vivo.Overall,we provide a promising leading compound for BRAF V600E or RAS mutant melanoma and colorectal cancer through dual targeting of CRAF and MEK1/2. | Penglei Wang Xuechao Jia Bingbing Lu Han Huang Jialin Liu Xuejiao Liu Qiong Wu Yamei Hu Pan Li Huifang Wei Tingting Liu Dengyun Zhao Lingwei Zhang Xueli Tian Yanan Jiang Yan Qiao Wenna Nie Xinli Ma Ruihua Bai Cong Peng Zigang Dong Kangdong Liu | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 1 |
| 20 | Acute toxicity and effects of 1-alkyl-3- methylimidazolium bromide ionic liquids on green algae显示文摘 | Ma Jianmin Cai Linlin Zhang Bangjun Hu Lingwei Li Xiaoyu Wang Jianji | 2010 | Ecotoxicology and Environmental Safety2010，73，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员