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| 1 | Soybean AP1 homologs control flowering time and plant height显示文摘Flowering time and plant height are key agronomic traits that directly affect soybean(Glycine max)yield.APETALA1(AP1)functions as a class A gene in the ABCE model for floral organ development,helping to specify carpel,stamen,petal,and sepal identities.There are four AP1 homologs in soybean,all of which are mainly expressed in the shoot apex.Here,we used clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein 9 technology to generate a homozygous quadruple mutant,gmap1,with loss-of-function mutations in all four GmAP1 genes.Under short-day(SD)conditions,the gmap1 quadruple mutant exhibited delayed flowering,changes in flower morphology,and increased node number and internode length,resulting in plants that were taller than the wild type.Conversely,overexpression of GmAP1a resulted in early flowering and reduced plant height compared to the wild type under SD conditions.The gmap1 mutant and the overexpression lines also exhibited altered expression of several genes related to flowering and gibberellic acid metabolism,thereby providing insight into the role of GmAP1 in the regulatory networks controlling flowering time and plant height in soybean.Increased node number is the trait with the most promise for enhancing soybean pod number and grain yield.Therefore,the mutant alleles of the four AP1 homologs described here will be invaluable for molecular breeding of improved soybean yield. | Liyu Chen Haiyang Nan Lingping Kong Lin Yue Hui Yang Qingsong Zhao Chao Fang Haiyang Li Qun Cheng Sijia Lu Fanjiang Kong Baohui Liu Lidong Dong | 2020 | Journal of Integrative Plant Biology2020，62，12： | 9 |
| 2 | MiR-223 promotes the doxorubicin resistance of colorectal cancer cells via regulating epithelialmesenchymal transition by targeting FBXW7显示文摘 | Jieqiong Ding Zhiwei Zhao Jia Song Binghua Luo Lingping Huang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，6： | 7 |
| 3 | Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitudes显示文摘Photoperiod responsiveness is a key factor limiting the geographic distribution of cultivated soybean and its wild ancestor.In particular,the genetic basis of the adaptation in wild soybean remains poorly understood.In this study,by combining whole-genome resequencing and genome-wide association studies we identified a novel locus,Time of Flowering 5(Tof5),which promotes flowering and enhances adaptation to high latitudes in both wild and cultivated soybean.By genomic,genetic and transgenic analyses we showed that Tof5 en-codes a homolog of Arabidopsis thaliana FRUITFULL(FUL).Importantly,further analyses suggested that different alleles of Tof5 have undergone parallel selection.The Tof5H1 allele was strongly selected by humans after the early domestication of cultivated soybean,while Tof5H2 allele was naturally selected in wild soybean,and in each case facilitating adaptation to high latitudes.Moreover,we found that the key flowering repressor E1 suppresses the transcription of Tof5 by binding to its promoter.In turn,Tof5 physically associates with the promoters of two important FLOWERING LOCUS T(FT),FT2a and FT5a,to upregulate their transcription and promote flowering under long photoperiods.Collectively,ourfindings provide insights into how wild soybean adapted to high latitudes through natural selection and indicate that cultivated soybean underwent changes in the same gene but evolved a distinct allele that was artificially selected after domestication. | Lidong Dong Qun Cheng Chao Fang Lingping Kong Hui Yang Zhihong Hou Yongli Li Haiyang Nan Yuhang Zhang Qingshan Chen Chunbao Zhang Kun Kou Tong Su Lingshuang Wang Shichen Li Haiyang Li Xiaoya Lin Yang Tang Xiaohui Zhao Sijia Lu Baohui Liu Fanjiang Kong | 2022 | Molecular Plant2022，15，2： | 5 |
| 4 | Systematic analysis of intron size and abundance parameters in diverse lineages显示文摘All eukaryotic genomes have genes with introns in variable sizes.As far as spliceosomal introns are concerned,there are at least three basic parameters to stratify introns across diverse eukaryotic taxa:size,number,and sequence context.The number parameter is highly variable in lower eukaryotes,especially among protozoan and fungal species,which ranges from less than4%to 78%of the genes.Over greater evolutionary time scales,the number parameter undoubtedly increases as observed in higher plants and higher vertebrates,reaching greater than 12.5 exons per gene in average among mammalian genomes.The size parameter is more complex,where multiple modes appear at work.Aside from intronless genes,there are three other types of intron-containing genes:half-sized,minimal,and size-expandable introns.The half-sized introns have only been found in a limited number of genomes among protozoan and fungal lineages and the other two types are prevalent in all animal and plant genomes.Among the size-expandable introns,the sizes of plant introns are expansion-limited in that the large introns exceeding 1000 bp are fewer in numbers and transposon-free as compared to the large introns among animals,where the larger introns are filled with transposable elements and appear expansion-flexible,reaching several kilobasepairs(kbp)and even thousands of kbp in size.Most of the intron parameters can be studied as signatures of the specific splicing machineries of different eukaryotic lineages and are highly relevant to the regulation of gene expression and functionality.In particular,the transcription-splicing-export coupling of eukaryotic intron dispensing leads to a working hypothesis that all intron parameters are evolved to be efficient and function-related in processing and routing the spliced transcripts. | WU JiaYan XIAO JingFa WANG LingPing ZHONG Jun YIN HongYan WU ShuangXiu ZHANG Zhang YU Jun | 2013 | Science China(Life Sciences)2013，56，10： | 4 |
| 5 | A universal pipeline for mobile mRNA detection and insights into heterografting advantages under chilling stress显示文摘Heterografting has long been used to enhance the chilling tolerance of temperature-sensitive crops,including watermelon,whose mechanism is known to involve bidirectional long-distance mRNA movements.Despite several studies reporting on mobile mRNA(mb-mRNA)profiles in plants,accurate identification of mb-mRNAs is challenging owing to an array of technical problems.Here,we developed a bioinformatical pipeline that took most of the known technical concerns into consideration and is considered to be a universal tool for mb-mRNA detection in heterografts.By applying this pipeline to a commercial watermelon–bottle gourd heterografting system,we detected 130 and 1144 mb-mRNAs upwardly and 167 and 1051 mb-mRNAs downwardly transmitted under normal and chilling-stress conditions,respectively.Quantitative real-time PCR indicated a high accuracy rate(88.2%)of mb-mRNA prediction with our pipeline.We further revealed that the mobility of mRNAs was not associated with their abundance.Functional annotation and classification implied that scions may convey the stress signal to the rootstock,subsequently triggering energy metabolism reprogramming and abscisic acid-mediated stress responses by upward movement of effective mRNAs,ultimately leading to enhanced chilling tolerance.This study provides a universal tool for mb-mRNA detection in plant heterografting systems and novel insights into heterografting advantages under chilling stress. | Ying Wang Lingping Wang Nailin Xing Xiaohua Wu Xinyi Wu Baogen Wang Zhongfu Lu Pei Xu Ye Tao Guojing Li Yuhong Wang | 2020 | Horticulture Research2020，7，1： | 3 |
| 6 | Agronomical selection on loss-of-function of GIGANTEA simultaneously facilitates soybean salt tolerance and early maturity显示文摘Salt stress and flowering time are major factors limiting geographic adaptation and yield productivity in soybean(Glycine max).Although improving crop salt tolerance and latitude adaptation are essential for efficient agricultural production,whether and how these two traits are integrated remains largely unknown.Here,we used a genome-wide association study to identify a major salt-tolerance locus controlled by E2,an ortholog of Arabidopsis thaliana GIGANTEA(GI).Loss of E2 function not only shortened flowering time and maturity,but also enhanced salt-tolerance in soybean.E2 delayed soybean flowering by enhancing the transcription of the core flowering suppressor gene E1,thereby repressing Flowering Locus T(FT)expression.An E2 knockout mutant e2^(CR) displayed reduced accumulation of reactive oxygen species(ROS)during the response to salt stress by releasing peroxidase,which functions in ROS scavenging to avoid cytotoxicity.Evolutionary and population genetic analyses also suggested that loss-of-function e2 alleles have been artificially selected during breeding for soybean adaptation to high-latitude regions with greater salt stress.Our findings provide insights into the coupled selection for adaptation to both latitude and salt stress in soybean;and offer an ideal target for molecular breeding of early-maturing and salt-tolerant cultivars. | Lidong Dong Zhihong Hou Haiyang Li Zhaobo Li Chao Fang Lingping Kong Yongli Li Hao Du Tai Li Lingshuang Wang Milan He Xiaohui Zhao Qun Cheng Fanjiang Kong Baohui Liu | 2022 | Journal of Integrative Plant Biology2022，64，10： | 3 |
| 7 | Neuroprotective effects of human telomerase reverse transcriptase on beta-amyloid fragment 25-35-treated human embryonic cortical neurons显示文摘BACKGROUND:Numerous current studies have suggested that human telomerase reverse transcriptase(hTERT) gene has neuroprotective effects and can inhibit apoptosis induced by various cytotoxic stresses;however,the mechanism of action remains unknown. OBJECTIVE:To evaluate the neuroprotective effects and possible mechanism of action of hTERT gene transfection in human embryonic cortical neurons treated with beta-amyloid fragment 25-35 (Aβ_(25-35)). DESIGN,TIME AND SETTING:The randomized,controlled and molecular biological studies were performed at the Department of Anatomy and Brain Research,Zhongshan School of Medicine,Sun Yat-sen University,China,from September 2005 to June 2008. MATERIALS:AdEasy-1 Expression System was gifted by Professor Guoquan Gao from Sun Yat-Sen University,China.Human cortical neurons were derived from 12-20 week old aborted fetuses,obtained from the Guangzhou Maternal and Child Health Hospital,China.Mouse anti-Cdk5 and mouse anti-p16 monoclonal antibodies(Lab Vision,USA),and mouse anti-hTERT monoclonal antibody(Epitomics,USA),were used in this study. METHODS:(1) Recombinant adenovirus vectors,encoding hTERT(Ad-hTERT) and green fluorescent protein(Ad-GFP),were constructed using the AdEasy-1 Expression System.Human embryonic cortical neurons in the Ad-hTERT group were transfected with Ad-hTERT for 1-21 days. Likewise,human embryonic cortical neurons in the Ad-GFP group were transfected with Ad-GFP for 1-21 days.Human embryonic cortical neurons in the control group were cultured as normal.(2) Human embryonic cortical neurons in the Ad-hTERT group were treated with 10μmol/L Aβ_(25-35) for 24 hours.Normal human embryonic cortical neurons treated with 10μmol/LAβ_(25-35) for 24 hours served as a model group.Human embryonic cortical neurons in the Ad-GFP and control groups were not treated with Aβ_(25-35). MAIN OUTCOME MEASURES:Expression of hTERT in human embryonic cortical neurons was evaluated by immunocytochemical staining and Western blot assay.Telomerase activity was measured using a PcR-based telomeric repeat amplification protocol(TRAP) ELISA kit.Neural activity in human embryonic cortical neurons was examined by MTT assay;apoptosis was measured using TUNEL assay;and Cdk5 and p16 protein expressions were measured by Westem blot. RESULTS:Expression of hTERT protein was significantly increased and peaked at day 3 post-transfection in the Ad-hTERT group.No hTERT expression was detected in the Ad-GFP and control groups.Telomerase activity was significantly greater in the Ad-hTERT group compared with the Ad-GFP and control groups(P<0.01).Compared with the control group,cell activity was significantly decreased(P<0.05),and cell apoptotic rate,Cdk5 and p16 expression were significantly increased(P<0.01) in the model group.Compared with the model group,cell activity was increased in the Ad-hTERT group,and peaked at day 3 post-transfection(P<0.05). Neuroprotective effects also peaked at day 3 post-transfection;and the apoptotic rate,Cdk5 and p16 expression significantly decreased(P<0.01). CONCLUSION:Expression of hTERT in human embryonic cortical neurons can relieve Aβ_(25-35)-induced neuronal apoptosis.The possible mechanism by which hTERT produces these neuroprotective effects may be associated with inhibition of Cdk5 and p16 expression. | Lingping Kong Lingzhi Wu Jie Zhang Yaping Liao Huaqiao Wang | 2009 | Neural Regeneration Research2009，4，6： | 3 |
| 8 | Study on Modification of Ultra-Stable Zeolite Prepared by Hydrothermal Method显示文摘极端马厩的沸石 DASY-0.0 被热水的方法在商业规模准备。它的结构进一步为在对待的 CP 被 XRF 分析并且描绘前后, .Thezeolite 取样的毛孔( CP )清洗经由处理被修改, XRD , NMR ,红外, BETand DTA.The 结果显示出那与由热水的过程的常规极端马厩的沸石 DASY-0.0prepared 比较, 修改CP 的沸石 SOYO 展出了更高的 relativecrystallinity ,更大的表面区域和毛孔体积,更高热的稳定性和包含的更少非结晶的非框架艾尔。 | Zhang Weilin Zhou Lingping Shen Shimin Li Zheng Zhu Yuxia Tian Huiping Long Jun | 2007 | China Petroleum Processing & Petrochemical Technology2007，9，2： | 2 |
| 9 | Anion Exchange Membrane Based on Interpenetrating Polymer Network with Ultrahigh Ion Conductivity and Excellent Stability for Alkaline Fuel Cell显示文摘A high-performance anion exchange membrane(AEM)is critical for the development of alkaline fuel cell.In this work,AEMs with an interpenetrating polymer network(IPN)are synthesized.An electron microscope clearly reveals a highly efficient“ion channel”network,which is constructed with a small amount of cation exchange groups.This specially designed ion channel leads to extraordinary hydroxide conductivity(e.g.,257.8 mS cm^(-1) at 80℃)of IPN AEMs at moderate ion exchange capacity(IEC=1:75 mmol g^(-1)),as well as excellent long-term alkaline stability at harsh condition which showed that 81%of original conductivity can be retained after a long time for 1248 hours.Moreover,a remarkable peak power density of 1.20 Wcm^(-2)(0.1 MPa backpressure)with nonprecious metal(FeNx-CNTs)as oxygen reduction reaction(ORR)catalyst in a fuel cell test was achieved.This work offers a general strategy to prepare high-performance AEMs based on IPN structure design. | Lingping Zeng Qian He Yunchuan Liao Shangyi Kuang Jianchuan Wang Wei Ding Qiang Liao Zidong Wei | 2020 | Research2020，，1： | 2 |
| 10 | PREPARATION AND CHARACTERIZATION OF A HIGH TEMPERATURE WATER—G显示文摘 | Zhou Lingping Guo | 1996 | 催化学报1996，17，5： | 2 |
| 11 | OXIDATIVE METHYL ATION OF TOL UENE WITH METHANE OVER BASIC ZEOL ITE CATAL YSTS显示文摘OXIDATIVEMETHYLATIONOFTOLUENEWITHMETHANEOVERBASICZEOLITECATALYSTS*ZhouLingping,TaoKeyi**,ZhangMinghui,SuMing,LiHexuan(Departm... | Zhou Lingping Gon | 1996 | 催化学报1996，17，5： | 2 |
| 12 | Atrial fibrillation is associated with accumulation of aging-related common type mitochondrial DNA deletion mutation in Human atrial tissue 显示文摘 | LingPing Lai Chien Chen Tsai MingJai Su | 2003 | Chest2003，123，2： | 1 |
| 13 | The mechanical performance and anti-corrosion behavior of diamond-like carbon film显示文摘 | G F Huang Zhou Lingping Huang Weiqing | 2003 | Diamond and Related Materials2003，12，： | 1 |
| 14 | Application of op- timal subset regressionin summer high temperature sprediction 显示文摘 | LI Lingping SHANG Kezheng QIAN Li | 2010 | Journal of Lanzhou University ( Natural Sciences)2010，46，6： | 1 |
| 15 | GIS-based Risk Zonation of Rainstorm Disaster in Guizhou Province显示文摘[Objective] This paper aimed to study the risk zoning of rainstorm in Guizhou based on GIS. [Method] Taking Guizhou as study area, 1 km×1 km grid data as evaluation unit, and by dint of daily precipitation in meteorological station in Guizhou from 1961 to 2008, the rainstorm risk zoning system was constructed from the aspects of disaster-stricken dangers, suffering flexibility, disaster environment sensitivity and disaster prevention or mitigation; based on the level analysis method to determine factor weight, the risk assessment model based on GIS was set up to evaluate the four sub-indicators and risks and to get the rainstorm disaster in Guizhou in the end. [Result] The risk assessment and zonation results showed a general trend that the risk level decreased from the central to all around. The low risk area distributed in the northwest of Guizhou province because of less heavy rains and high capacity of rainstorm disaster resistant, while high risk area mainly distributed in the west-central of Guizhou due to concentration rainstorms, large terrain undulation and low coverage rate of forest. Especially, according to Anshun, the high risk area took up 98.02% of the city, and the Gangwu County, where a super-large geological disaster concurred in 2010 is located at the high risk area, which showed that the risk assessment coincided with the actual situation. [Conclusion] The study provided theoretical basis for the macro disaster prevention and disaster mitigation plan. | CHEN Zhen-hong1, YANG Yi2, LIU Fang3, 4, WANG Xing-ju1 1. Anshun Meteorological Bureau in Guizhou Province, Anshun 561000, China 2. Lingping County Meteorological Bureau in Guizhou Province, Lingping 5573001, China 3. Geographic Science and Resources Research Institute of Chinese Academy of Sciences, Beijing 100101, China 4. Graduate University of Chinese Academy of Sciences, Beijing 100049, China | 2011 | Meteorological and Environmental Research2011，2，4： | 1 |
| 16 | Direct synthesis,characterization of Cu-SBA-15 and its high catalytic activity in hydroxylation of phenol by H2O2显示文摘 | Lingping Wang Aiguo Kong Bo Chen | 2005 | Journal of Molecular Catalysis A:Chemical2005，230，12： | 1 |
| 17 | Gas path fault diagnosis system of aero-en- gine based on grey relationship degree 显示文摘 | JIANG Lingping | 2011 | Procedia Engi- neering2011，15，： | 1 |
| 18 | Industrial Preparation and Acid Resistance of Ultra-stable Y Zeolite with Small Cell Size Produced by Gas-phase Method显示文摘Small-cell HSY-S zeolite prepared by the gas-phase ultra-stable method had been researched and developed,and industrial preparation tests of HSY-S have been successfully carried out for the first time.The acid resistance of industrially prepared HSY-S was investigated by acid solutions with different pH values.The structures and properties of HSY-S and its acid-treated samples were characterized by XRD,XRF,BET,and IR.Results show that the HSY-S samples have the characteristics of high crystallinity,good stability,large specific surface area,and good acid resistance. | Zhang Jing Jia Jishun Sha Hao Lu Guanqun Yan Jiasong Wang Shengji Zhou Lingping | 2022 | China Petroleum Processing & Petrochemical Technology2022，24，2： | 1 |
| 19 | Cooperative Branding For Rual Destinations 显示文摘 | LINGPING A Cai | 2002 | Annals of tourism research2002，，： | 1 |
| 20 | Direct synthesis,characterization of Cu-SBA-15 and its high catalytic activity inhydroxylation of phenol by H2O2显示文摘 | Wang Lingping Kong Aiguo Chen Bo | 2005 | J Mol Catal A:Chemical2005，230，12： | 1 |
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