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| 1 | mTOR/miR-145-regulated exosomal GOLM1 promotes hepatocellular carcinoma through augmented GSK-3b/MMPs显示文摘Golgi membrane protein 1(GOLM1/GP73)is a serum marker of hepatocellular carcinoma(HCC).We have previously shown that mTOR promoted tumorigenesis of HCC through stimulating GOLM1 expression.In this study,we demonstrated that the mammalian target of rapamycin(mTOR)was a negative regulator of microRNA-145(miR-145)expression.miR-145 inhibited GOLM1 expression by targeting a coding sequence of GOLM1 gene.GOLM1 and miR-145 were inversely correlated in human HCC tissues.GOLM1-enriched exosomes activated the glycogen synthase kinase-3β/matrix metalloproteinases(GSK-3β/MMPs)signaling axis of recipient cells and accelerated cell proliferation and migration.In contrast,miR-145 suppressed tumorigenesis and metastasis.We suggest that mTOR/miR-145/GOLM1 signaling pathway should be targeted for HCC treatment. | Xiaochen Gai Bufu Tang Fangming Liu Yuting Wu Fang Wang Yanling Jing Fuqiang Huang Di Jin Ling Wang Hongbing Zhang | 2019 | Journal of Genetics and Genomics2019，46，5： | 13 |
| 2 | Effect of Depot Medroxyprogesterone Acetate (DMPA) on Bone Mineral Density in Women of Reproductive Age显示文摘Objective To compare bone mineral density (BMD) between users of intramuscular depot medroxyprogesterone acetate(DMPA) and nonhormonal control subjects. Methods The study included 68 women aged between 25 and 40 years using depot medroxyprogesterone acetate for 24 months and 59 women aged between 25 and 40 years using nonhormonal contraception as control subjects. BMD of the lumbar spine and femoral neck were obtained using dual energy X-ray absorptiometry. Results At 24 months of treatment, as compared with baseline, the mean BMD in lumbar spine and femoral neck was decreased by 5.5% and 5.9%, respectively. Lumbar spine and femoral neck BMD in women who used DMPA were significantly decreased compared with the subjects in nonuser (P<0.001). Conclusion These results show BMD declined during using DMPA in women aged 25-40 years old. | Ling GAI Jing-lu ZHANG Hui-zhen ZHANG Ping GAI Yong-hong LIU | 2009 | Journal of Reproduction and Contraception2009，20，1： | 6 |
| 3 | Effects of the crude extract of Polygala tenuifolia Willd on human sperm in vitro显示文摘The aim of the present study is to analyze sperm membrane changes and the spermicidal effect in treatment with the crude extract from Polygala tenuifolia Willd (PTW) in vitro.The root of PTW was extracted in distilled water.Normal human spermatozoa were used to assess the spermicidal activity (Sander-Cramer assay) of the extract from the PTW root.The hypo-osmotic swelling (HOS) test and the eosin Y (EY) staining were used to detect the integrity of sperm membrane and vitality.The sperm chromatin dispersion (SCD) test was performed to determine sperm DNA integrity.N-9 was used as a reference standard and semen added to physiological saline was used as the control.Semen samples were donated by 42 healthy fertile men.The crude extract from the root of PTW could immobilize and kill 100% spermatozoa within 20 s in vitro at the concentrations of 20.0 and 10.0 mg/ml;at the concentration of 5.0 mg/ml,spermatozoa were immobilized in (39.5±3.2) s.In the groups of the crude extract from the root of PTW and N-9 solution,the rate of the normal HOS (tails swollen) and the white head (unstained) was 0%,and the rate of the abnormal HOS (tails unswollen) and red head (stained) was 100%.Sperm DNA fragmentation showed no change in exposure to the crude extract from the root of PTW and N-9 solution.The sperm revival test did not show any spermatozoa that recovered their motilities.The rapid spermicidal activity of the crude extract from the root of PTW in vitro may occur by the disruption of the sperm membrane integrity. | Yi QIU Lei-guang WANG Yi-fang JIA Dan-tong YANG Mei-hua ZHANG Yan-ping ZHANG Li-hong ZHANG Ling GAI | 2011 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2011，12，6： | 4 |
| 4 | Effect of depot medroxyprogesterone acetate on bone mineral density in adolescent women显示文摘 | Zhang Mei-hua Zhang Wei Zhang Ai-dong Yang Yan Gai Ling | 2013 | Chinese Medical Journal2013，，21： | 3 |
| 5 | Effect of Two Kinds of Different Combined Oral Contraceptives Used on Bone Mineral Density in Women of Reproductive Age显示文摘Objective To compare bone mineral density (BMD) among users of ethinylestradiol/desogestrel, ethinylestradiol/cyproterone acetate and nonhormonal control subjects. Methods The study included 154 women aged 25-40 years. Forty-six women were using ethinylestradiol/desogestrel (group A) and 55 women using ethinylestradiol/cyproterone acetate (group B) for 24 months and 53 women using nonhormonal contraception as control subjects (group C). BMD of the lumbar spine and femoral neck were obtained using dual energy X-ray absorptiometry, comparing mean BMD changes in combined oral contraceptives (COCs) users with nonusers. Results For 24 months of treatment, lumbar spine and femoral neck BMD values in women who used ethinylestradiol/desogestrel and ethinylestradiol/cyproterone acetate were not significantly different compared with the baseline and the values of subjects in nonuser (P>0.05). Conclusion There was no statistical difference in BMD between the users of ethinylestradiol/desogestrel or ethinylestradiol/cyproterone acetate and nonusers. | Rui-jian LIU Ling GAI Yi-fang JIA Ping GAI Xiao-jie YU | 2011 | Journal of Reproduction and Contraception2011，22，3： | 3 |
| 6 | Effect of Combined Oral Contraceptive Use on Bone Mineral Density in Adolescent Females显示文摘Objective To compare adolescents'bone mineral density (BMD) between users of combined oral contraceptive (Marvelon, desogestrel/ethinylestradiol) and nonhormonal control subjects. Methods The study included 127 women who aged between 16 and 18 years using Marvelon for 24 months and 115 women who aged between 16 and 18 years using nonhormonal contraception as control subjects. BMD of the lumbar spine and femoral neck were obtained using dual energy X-ray absorptiometry. Results After 24 months of Marvelon use, as compared with baseline, the mean BMD in lumbar spine and femoral neck were decreased by 0.30% and 0.61%, respectively. While in the nonusers group, the mean BMD were increased by 1.88% and 1.10%, respectively. Lumbar spine and femoral neck BMD in women who used Marvelon were not significantly different compared with the subjects who used nonhormonal contraception (P>0.05). Conclusion Two years of Marvelon use had no significant effect on BMD, but it remains unknown whether longer than 2 years of use has a significant adverse effect on the attainment of peak bone mass. | Ling GAI Xia LIU Ping GAI Ai-dong ZHANG Ping JIANG Wei ZHANG | 2012 | Journal of Reproduction and Contraception2012，23，3： | 2 |
| 7 | Fusarielin E,a new antifungal antibiotic from Fusarium sp.显示文摘新抗真菌的抗菌素, fusarielin E,从导出水兵的真菌镰刀霉 sp 被孤立。它的结构根据各种各样的 NMR 分光镜的分析和 HR-FAB-MS 被建立。Fusarielin E 对 Pyricularia oryzae 显示了重要生物效能。 | Yue Gai Ling Ling Zhao Chang Qi Hu Hui Ping Zhang | 2007 | Chinese Chemical Letters2007，18，8： | 2 |
| 8 | LncRNA GAS5 enhances tumor stem cell-like medicated sensitivity of paclitaxel and inhibits epithelial-to-mesenchymal transition by targeting the miR-18a-5p/STK4 pathway in prostate cancer显示文摘The onset of prostate cancer(PCa)is often hidden,and recurrence and metastasis are more likely to occur due to chemotherapy resistance.Herein,we identified downregulated long noncoding RNA(lncRNA)growth arrest-specific 5(GAS5)in PCa that was associated with metastasis and paclitaxel resistance.GAs5 acted as a tumor suppressor in suppressing the proliferation and metastasis of paclitaxel-resistant PCa cells.GAS5 overexpression in vivo inhibited the tumor growth of xenografts and elevated PCa sensitivity to paclitaxel.Combination of GAS5 and paclitaxel treatment showed great potential in PCa treatment.Moreover,mechanistic analysis revealed a novel regulatory network of GAS5/miR-18a-5p/serine/threonine kinase 4(STK4)that inhibits epithelial-to-mesenchymal transition(EMT)and enhances tumor stem cell-like-mediated sensitivity to paclitaxel in PCa.These findings provide a novel direction for the development of a potential adjunct to cancer chemotherapy that aims to improve the sensitivity of chemotherapy drugs in PCa. | Ting-Ting Lu Xia Tao Hua-Lei Li Ling Gai Hua Huang Feng Li | 2022 | Asian Journal of Andrology2022，24，6： | 2 |
| 9 | Effect of Paris saponin on antitumor and immune function in U14 tumor-bearing mice显示文摘 | Guang Lie C Wei Shi G Gai Ling H | 2013 | Afr J Tradit Complement Altern Med2013，10，3： | 1 |
| 10 | The use of miR122 and its target sequence in adeno-associated virus-mediated trichosanthin gene therapy显示文摘Objective:Plant-derived cytotoxic transgene expression,such as trichosanthin(tcs),regulated by recombinant adeno-associated virus(r AAV)vector is a promising cancer gene therapy.However,the cytotoxic transgene can hamper the vector production in the r AAV producer cell line,human embryonic kidney(HEK293)cells.Here,we explored micro RNA-122(miR122)and its target sequence to limit the expression of the cytotoxic gene in the r AAV producer cells.Methods:A miR122 target(122 T)sequence was incorporated into the 30 untranslated region of the tcs c DNA sequence.The firefly luciferase(fluc)transgene was used as an appropriate control.Cell line HEK293-mir122 was generated by the lentiviral vector-mediated genome integration of the mir122 gene in parental HEK293 cells.The effects of miR122 overexpression on cell growth,transgene expression,and r AAV production were determined.Results:The presence of 122 T sequence significantly reduced transgene expression in the miR122-enriched Huh7 cell line(in vitro),fresh human hepatocytes(ex vivo),and mouse liver(in vivo).Also,the normal liver physiology was unaffected by delivery of 122 T sequence by r AAV vectors.Compared with the parental cells,the miR122-overexpressing HEK293-mir122 cell line showed similar cell growth rate and expression of transgene without 122 T,as well as the ability to produce liver-targeting r AAV vectors.Fascinatingly,the yield of r AAV vectors carrying the tcs-122 T gene was increased by 77.7-fold in HEK293-mir122 cells.Moreover,the tcs-122 T-containing r AAV vectors significantly reduced the proliferation of hepatocellular carcinoma cells without affecting the normal liver cells.Conclusion:HEK293-mir122 cells along with the 122 T sequence provide a potential tool to attenuate the cytotoxic transgene expression,such as tcs,during r AAV vector production. | Gai Ran Xi-lin Feng Yi-lin Xie Qing-yun Zheng Peng-peng Guo Ming Yang Ying-lu Feng Chen Ling Li-qing Zhu Chen Zhong | 2021 | Journal of Integrative Medicine2021，19，6： | 1 |
| 11 | Mass transfer techniques for large-scaleand high-density microLED arrays显示文摘Inorganic-based micro light-emitting diodes (microLEDs) offer more fascinating properties and unique demands in next-generation displays. However, the small size of the microLED chip (1–100 µm) makes it extremely challenging for high efficiency and low cost to accurately, selectively, integrate millions of microLED chips. Recent impressive technological advances have overcome the drawbacks of traditional pick-and-place techniques when they were utilized in the assembly of microLED display, including the most broadly recognized laser lift-off technique, contact micro-transfer printing (µTP) technique, laser non-contact µTP technique, and self-assembly technique. Herein, we firstly review the key developments in mass transfer technique and highlight their potential value, covering both the state-of-the-art devices and requirements for mass transfer in the assembly of the ultra-large-area display and virtual reality glasses. We begin with the significant challenges and the brief history of mass transfer technique, and expand that mass transfer technique is composed of two major techniques, namely, the epitaxial Lift-off technique and the pick-and-place technique. The basic concept and transfer effects for each representative epitaxial Lift-off and pick-and-place technique in mass transfer are then overviewed separately. Finally, the potential challenges and future research directions of mass transfer are discussed. | Furong Chen Jing Bian Jinlong Hu Ningning Sun Biao Yang Hong Ling Haiyang Yu Kaixin Wang Mengxin Gai Yuhang Ma YongAn Huang | 2022 | International Journal of Extreme Manufacturing2022，4，4： | 1 |
| 12 | 5′-Hydroxyzearalenol,a new β-resorcylic macrolide from Fusarium sp. 05ABR26显示文摘新尾 - resorcylic macrolide, 5 鈥 ? hydroxyzearalenol (1 ) ,从导出水兵的真菌镰刀霉 sp 的文化肉汤被孤立。05ABR26。三已知的混合物, zearalenone (2 ) , 8 鈥 ? hydroxyzearalenone (3 ) 和 zearalenol (4 ) 也被孤立。结构和 1 的相对立体化学根据分光镜的数据和单个水晶的 X 光检查衍射数据被阐明。化合物 2 显示的有势力对有 6.25 渭 g /mL 的 MIC 价值的 Pyricularia oryzae 的禁止的活动,当化合物 3 是更不活跃的时;然而, 1 和 4 没显示出明显的活动。 | Ling Ling Zhao Yue Gai Hisayoshi Kobayashi Chang Qi Hu Hui Ping Zhang | 2008 | Chinese Chemical Letters2008，19，9： | 1 |
| 13 | Effective production and purification of the glycosylated TSOL18 antigen,which is protective against pig cysticercosis显示文摘 | CAI Xue-peng YUAN Gai ling ZHENG Ya-dong | 2008 | Infect Immun2008，76，2： | 1 |
| 14 | Effect of depot medroxyprogesterone acetate(DMPA) on bone mineral density(BMD) and evaluating changes in BMD after discontinuation of DMPA in adolescent women显示文摘Objective To compare bone mineral density(BMD) between users of intramuscular depot medroxyprogesterone acetate(DMPA) and nonhormonal subjects and evaluated the changes in BMD after discontinuation of DMPA.Methods The study included 102 women aged 16-18 years using DMPA for 24 months and 97 same-age nonusers. BMD of the lumbar spine and femoral neck were measured using dual-energy X-ray absorptiometry. The mean BMD values were compared between DMPA users(DMPA group) and nonusers(control group) and the changes of BMD during 36 months after discontinuation of DMPA were observed.Results Mean BMD at the spine and femoral neck did not differ significantly between DMPA group and control group over 12 months, but the BMD values at both anatomical sites were significantly lower in DMPA group than in control group after 24 months treatment. After DMPA discontinuation, the mean BMD values in DMPA users increased substantially. At 24 months after DMPA discontinuation, there were no significant differences between DMPA group and control group. But the values of the lumbar spine and femoral neck BMD in DMPA group were still 1.70% and 1.87%, respectively,below nonusers at 36 months after DMPA discontinuation.Conclusion The use of DMPA for short-term(≤12 months) had no significant effects on BMD at spine and femoral neck, but long-term exposure to DMPA had significantloss in BMD in adolescents. Bone loss occurring with DMPA use is reversible after DMPA discontinuation. | Cheng-wu SHEN Ling GAI Li-hong ZHANG Lin-lin WANG Hai-qing DING Hui JIANG | 2015 | Journal of Reproduction and Contraception2015，26，3： | 0 |
| 15 | Research on Pattern Matching Method of Multivariate Hydrological Time Series显示文摘The existing pattern matching methods of multivariate time series can hardly measure the similarity of multivariate hydrological time series accurately and efficiently.Considering the characteristics of multivariate hydrological time series,the continuity and global features of variables,we proposed a pattern matching method,PP-DTW,which is based on dynamic time warping.In this method,the multivariate time series is firstly segmented,and the average of each segment is used as the feature.Then,PCA is operated on the feature sequence.Finally,the weighted DTW distance is used as the measure of similarity in sequences.Carrying out experiments on the hydrological data of Chu River,we conclude that the pattern matching method can effectively describe the overall characteristics of the multivariate time series,which has a good matching effect on the multivariate hydrological time series. | Zhen Gai Yuansheng Lou Feng Ye Ling Li | 2017 | 国际计算机前沿大会会议论文集2017，，1： | 0 |
| 16 | Reducing Multivalued Discrete Variables in Solving Separable Task Assignment Problems显示文摘In this paper,we introduce the separable task assignment problem(STAP)in which n separable tasks are assigned to m agents subject to agents’capacity constraints.The objective is to minimize the costs that occur during the manufacturing and the communication between agents.A task is separable if it can be divided into two pieces,and both of them can be assigned individually or together to any agents.A separable task is considered as being assigned if and only if its two pieces are both assigned.Since several discrete(ternary)variables may be involved in STAP modeling,computing the problem in a reasonable time period is not an easy work.We replace the ternary variables by binary and continuous variables through extending the logarithmic method introduced by Li et al.(INFORMS J Comput 25(4):643–653,2012)and Vielma et al.(Oper Res 58(2):303–315,2010).Our numerical experiments demonstrate that the newly generated model performs well in solving difficult separable task-assignment problems for pretty large scale of instance sizes. | Ling Gai Qing-Wei Jin Yuan Tian Yao-Huei Huang | 2016 | Journal of the Operations Research Society of China2016，4，1： | 0 |
| 17 | Study on tongue coating microbes of bitter taste,sticky and greasy taste in chronic atrophic gastritis显示文摘OBJECTIVE:To objectively reveal the relationship between tongue coating microbes and bitter taste,sticky and greasy taste in chronic atrophic gastritis(CAG)patients.METHODS:16S rRNA high-throughput sequencing was used to detect bacterial diversity and community composition of tongue coating microbes from samples of CAG patients.LEfSe algorithm was used for discovering the different tongue coating microbes in CAG patients with or without bitter taste,also that in CAG patients with or without sticky and greasy taste.RESULTS:We respectively compared the features of tongue coating microbes in bitter taste,sticky and greasy taste of CAG patients.At the genus level,25 tongue coating microbes were significantly different in CAG patients with bitter taste or without bitter taste;17 tongue coating microbes were significantly different in CAG patients with sticky and greasy taste or without sticky and greasy taste.Campylobacter and Rothia were closely related to CAG patients with bitter taste.Enterococcus,Serratia,Leptotrichia and Selenomonas were closely related to CAG patients with stick and greasy taste.CONCLUSION:Campylobacter and Rothia possibly contribute to bitter taste of CAG patients,and Enterococcus,Serratia,Leptotrichia and Selenomonas contribute to stick and greasy taste of CAG patients,which is potential for the diagnosis and treatment of CAG. | CAO Yang GAI Xiao ZHENG Yixin Guo Benqiong NIU Jingbin QIAN Peng LING Jianghong LIU Guoping ZHENG Yu | 2023 | Journal of Traditional Chinese Medicine2023，43，1： | 0 |
| 18 | Approximation Algorithm for the Balanced 2-Correlation Clustering Problem显示文摘The Correlation Clustering Problem(CorCP) is a significant clustering problem based on the similarity of data.It has significant applications in different fields,such as machine learning,biology,and data mining,and many different problems in other areas.In this paper,the Balanced 2-CorCP(B2-CorCP) is introduced and examined,and a new interesting variant of the CorCP is described.The goal of this clustering problem is to partition the vertex set into two clusters with equal size,such that the number of disagreements is minimized.We first present a polynomial time algorithm for the B2-CorCP on M-positive edge dominant graphs(M≥ 3).Then,we provide a series of numerical experiments,and the results show the effectiveness of our algorithm. | Sai Ji Dachuan Xu Donglei Du Ling Gai Zhongrui Zhao | 2022 | Tsinghua Science and Technology2022，27，5： | 0 |
| 19 | Laser projection proximity transfer for deterministic assembly of microchip arrays at scale显示文摘Deterministic assembly techniques that enable programmatic and massively parallel integration of chips are essential for the development of novel electronic systems such as micro LED displays.However,large-area integration of ultrathin micro-chips with high yield and transfer accuracy remains a great challenge due to the difficulties in selective transfer,adhesion switchability,and transfer deviation.Here,a“laser projection proximity transfer(Laser PPT)”technique is presented for the deterministic assembly of microchip arrays at scale.One of the remarkable features is that the transfer status between the chip and the receiver substrate evolves from the original non-contact mode to contact mode for high-precision transfer,which overcomes the strict requirements of the flatness of stamp and substrate in contact-style transfer,and flight deviation of microchip array in noncontact-style transfer.Another feature is the rapid modulation of interfacial adhesion for reliable transfer via the use of thermally expandable microspheres to form microstructures and combining with a laser-induced blister.The adhesion regulation range is over 20 times without any damage to chip arrays.The results show that the transfer accuracy has been improved substantially with a minimum relative error of~0.5%.Combined with a laser beam projection system,demonstrations of Laser PPT for selective assembly of fragile objects onto challenging non-adhesive/cured surfaces in batch illustrate its potential in the highprecision integration of microscale chips at scale. | HU JinLong CHEN FuRong BIAN Jing SUN NingNing WANG KaiXin LING Hong YU HaiYang GAI MengXin XU LiZhi HUANG YongAn | 2022 | Science China(Technological Sciences)2022，65，9： | 0 |
| 20 | Changes in bone mineral density among women aged 19-24 years using injectable contraceptive depot medroxyprogesterone acetate显示文摘Objective To compare bone mineral density(BMD) between users of intramuscular depot medroxyprogesterone acetate(DMPA) and nonhormonal control subjects.Methods The study included 87 women aged between 19 and 24 years using DMPA(DMPA group) for 24 months and 83 same-age women using nonhormonal contraception as control group. BMD of the lumbar spine and femoral neck were measured using dual-energy X-ray absorptiometry, comparing mean BMD changes in DMPA group with the control.Results After 24 months of DMPA treatment, as compared with baseline, the mean BMD in lumbar spine and femoral neck were decreased by 2.67% and 2.71%, respectively.The mean lumbar spine and femoral neck BMD in DMPA group at 24 months were not significantly different compared with baseline(P=0.080 and P=0.076, respectively). In comparison, in control group, there was a trend toward increasing BMD. After 24 months of observation, the mean percentage changes from baseline in lumbar spine and femoral neck BMD were increased by 1.24% and 1.30%, respectively. There was no significantly difference compared with baseline(P=0.436 and P=0.373, respectively).Mean BMD at the spine and femoral neck did not differ significantly between DMPA users and nonusers over 12 months(P=0.153 and P=0.140, respectively), but the BMD at both anatomical sites was significantly lower in DMPA group than in the controlafter 24 months of treatment(P=0.012 and P=0.008, respectively).Conclusion The use of DMPA for short-term(≤12-month) had no significant effects on BMD at spine and femoral neck, but long-term exposure to DMPA had significant loss in BMD in women aged between 19 and 24 years. | Ling GAI Li-hong ZHANG Xue-mei LV Bin WEI Hai-qing DING Jin-yun LIU Hui JIANG | 2015 | Journal of Reproduction and Contraception2015，26，2： | 0 |
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