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| 1 | Norway spruce(Picea abies) laccases:Characterization of a laccase in a lignin-forming tissue culture显示文摘Secondarily thickened cell walls of water-conducting vessels and tracheids and support-giving sclerenchyma cells contain lignin that makes the cell wal s water impermeable and strong. To what extent laccases and peroxidases contribute to lignin biosynthesis in muro is under active evaluation. We performed an in silico study of Norway spruce(Picea abies(L.)Karst.) laccases utilizing available genomic data. As many as 292 laccase encoding sequences(genes, gene fragments, and pseudogenes) were detected in the spruce genome. Out of the 112 genes annotated as laccases, 79 are expressed at some level. We isolated five full-length laccase c DNAs from developing xylem and an extracellular lignin-forming cell culture of spruce. In addition, we purified and biochemically characterized one culture medium laccase from the lignin-forming cell culture.This laccase has an acidic p H optimum(p H 3.8–4.2) for coniferyl alcohol oxidation. It has a high af finity to coniferyl alcohol with an apparent K m value of 3.5 m M; however, the laccase has a lowe catalytic ef ficiency(V max/K m) for coniferyl alcohol oxidation compared with some purified culture medium peroxidases. The properties are discussed in the context of the information already known about laccases/coniferyl alcohol oxidases o coniferous plants. | Sanna Koutaniemi Heli A.Malmberg Liisa K.Simola Teemu H.Teeri Anna K?rk?nen | 2015 | Journal of Integrative Plant Biology2015，57，4： | 4 |
| 2 | 全膝关节置换翻修术的临床疗效及影响因素分析显示文摘目的通过对芬兰关节置换登记系统的资料分析,探讨全膝关节翻修术的治疗结果,并以再次翻修为终点评估翻修假体生存的预测指标。方法1990年至2002年芬兰国家关节置换登记系统共录入全膝关节翻修术2637例,以再次翻修为统计止点,对上述完整材料进行各种统计学分析,主要包括单因素分析中的Kaplan-Meier生存分析,Log-Rank检验,Cox回归分析及模型诊断。结果全膝关节翻修术后假体2年生存率为95%(1874膝)(95%置信区间,94%~96%),5年为89%(944膝)(95%置信区间,88%~90%),10年为79%(141膝)(95%置信区间,78%~81%)。多变量回归分析表明,假体生存最显著的预测指标是患者的年龄及初次全膝关节置换术后的使用时间(即初次与翻修手术间隔的时间)。预测生存率的其他重要指标有第1次翻修的年份及翻修原因。结论预测全膝关节翻修术后假体生存率较好的因素有:患者年龄在70岁以上、初次关节置换术后5年或以上施行翻修以及无髌骨半脱位。同时,随着年龄的增长、疾病的加重(骨关节炎和类风湿关节炎)及其治疗(初次全膝关节置换术),均可以减少关节活动量,加上医师不主张对老年患者施行手术,使得老年患者的再翻修率有所降低。 | 盛璞义 张紫机 廖威明 康焱 雷磊 杨子波 张浩 傅明 何爱珊 Liisa Konttinen Matti Lehto Yrj T Konttinen | 2008 | 中华关节外科杂志(电子版)2008，2，6： | 4 |
| 3 | Human biofield therapy does not affect tumor size but modulates immune responses in a mouse model for breast cancer显示文摘OBJECTIVE:To assess the effect of human biofield therapy, an integrative medicine modality, on the development of tumors and metastasis, and immune function in a mouse breast cancer model. METHODS: Mice were injected with 66cl4 mammary carcinoma cells. In study one, mice received biofield therapy after cell injection. In study two, mice were treated by the biofield practitioner only prior to cell injection. Both studies had two control groups of mock biofield treatments and phosphatebuffered saline injection. Mice were weighed and tumor volume was determined. Blood samples were collected and 32 serum cytokine/chemokine markers were measured. Spleens/popliteal lymph nodes were isolated and dissociated for fluorescent-activated cell sorting(FACS) analysis of immune cells or metastasis assays in cell culture.RESULTS: No significant differences were found in weight, tumor size or metastasis. Significant effects were found in the immune responses in study one but no additional effects were found in study two. In study one, human biofield treatment significantly reduced percentage of CD4^+CD44lo CD25^+ and percentage of CD8^+ cells, elevated by cancer in the lymph nodes, to control levels determined by FACS analysis. In the spleen, only CD11b^+ macrophages were increased with cancer, and human biofield therapy significantly reduced them. Of 11 cytokines elevated by cancer, only interferon-g, interleukin-1, monokine induced by interferon-g, interleukin-2 and macrophage inflammatory protein-2 were significantly reduced to control levels with human biofield therapy.CONCLUSION: Human biofield therapy had no significant effect on tumor size or metastasis but produced significant effects on immune responses apparent in the down-regulation of specific lymphocytes and serum cytokines in a mouse breast cancer model. | Gloria Gronowicz Eric R.Secor Jr. John R.Flynn Liisa T.Kuhn | 2016 | Journal of Integrative Medicine2016，14，5： | 3 |
| 4 | Helicobacter spp. Other than H . pylori显示文摘 | Mirko Rossi Marja‐Liisa H?nninen | 2012 | Helicobacter2012，，： | 2 |
| 5 | Effects of the viability of Lactobacillus rhamnosus GG on rotavirus infection in neonatal rats显示文摘AIM:To study the effects of live and dead Lactobacillus rhamnosus GG(GG) on rotavirus infection in a neonatal rat model.METHODS:At the age of 2 d,suckling Lewis rat pups were supplemented with either live or dead GG and the treatment was continued daily throughout the experi-ment.At the age of 5 and 6 d the pups received oral rotavirus(RV) SA-11 strain.The pups were sacrificed at the age of 7 or 8 d by decapitation.The gastrointestinal tract was removed and macroscopic observations were done.The consistency of feces in the colon was classified using a four-tier system.RV was detected from the plasma,small intestine,colon and feces by real-time quantitative polymerase chain reaction(PCR).RESULTS:In this neonatal rat model,RV induced a mild-to-moderate diarrhea in all except one pup of the RV-inoculated rats.RV moderately reduced body weight development from day 6 onwards.On day 7,after 2 d of RV infection,live and dead GG groups gained significantly more weight than the RV group without probiotics [36%(P = 0.001) and 28%(P = 0.031),respectively].In addition,when compared with the RV control group,both live and dead GG reduced the weight ratio of colon/animal body weight to the same level as in the healthy control group,with reductions of 22%(P = 0.002) and 28%(P < 0.001),respectively.Diarrhea increased moderately in both GG groups.However,the diarrhea incidence and severity in the GG groups were not statistically significantly different as compared with the RV control group.Moreover,observed diarrhea did not provoke weight loss or death.The RV control group had the largest amount of RV PCR-positive samples among the RV-infected groups,and the live GG group had the smallest amount.Rats receiving live GG had significantly less RV in the colon(P = 0.027) when compared with the RV control group.Live GG was also more effective over dead GG in reducing the quantity of RV from plasma(P = 0.047).CONCLUSION:Both live and dead GG have beneficial effects in RV infection.GG may increase RV clearance from the body and reduce colon swelling. | Hanna Ventola Liisa Lehtoranta Mari Madetoja Marja-Leena Simonen-Tikka Leena Maunula Merja Roivainen Riitta Korpela Reetta Holma | 2012 | World Journal of Gastroenterology2012，18，41： | 2 |
| 6 | 自我认知的能量显示文摘一个普通的工作日会带来很多消极的感觉。比如:你在进入办公室之前就知道今天的日程满满的,这可真让人感到压抑。跟难缠的客户打交道可能引起焦虑,展示新产品会让我们感到紧张。 | Liisa Kokko 李之澜(编译) | 2007 | 中外管理2007，，10： | 2 |
| 7 | The Economic Value of Urban Forest Amenities:An Application of the Contingent Valuation Method显示文摘 | Tyrv(a)inen Liisa V(a)(a)n(a)nen Hannu | 1998 | Landscape and Urban Planning1998，43，： | 1 |
| 8 | Indoor air aerosol model: the effect of outdoor air, filtration and ventilation on indoor concentrations 显示文摘 | Markku Kulmala Ari Asmi Liisa Pirjola | 1999 | Atmospheric Environment1999，33，1417： | 1 |
| 9 | The Amenity Value of the Urban Forest:An Application of the Hedonic Pricing Method显示文摘 | Tyrv(a)inen Liisa | 1997 | Landscape and Urban Planning1997，37，： | 1 |
| 10 | Stress inhibits the proliferation of granule cell precursors in the developing dentate gyrus显示文摘 | Galea Liisa AM Gould E | 1998 | Int J Dev Neurosci1998，16，34： | 1 |
| 11 | The effect of phytic acid and some natural chelating agents on the solubility of mineral elements in oat bran显示文摘 | Paivie Liisa V Maija Y | 2003 | Food Chemistry2003，，80： | 1 |
| 12 | Linear differential equations with solution of finite iterated order显示文摘 | LIISA Kinnunen | 1988 | Southeast Asian Bulletin of Mathematics1988，22，： | 1 |
| 13 | 'You Just Have to Make the Pain Go Away'---Children's experiences of pain man agement显示文摘 | Riitta- Liisa Kortesluoma Merja Nikkonen | 2008 | Pain Management Nursing2008，9，4： | 1 |
| 14 | Efficient Evacuation Meth- ods in Tall Buildings显示文摘 | Marja- Liisa Siikonen Henri Hakonen | 2003 | Elevator World2003，51，7： | 1 |
| 15 | Red-cell ICAM-d is a ligand for the monocyte/macrophage integrin CD11 c/CD18 :Char- acterization of the binding sites on ICAM-4 显示文摘 | Eveliina Ihanns Liisa M Votila | 2007 | Blood2007，109，： | 1 |
| 16 | Aggressive Problem- Solving Strategies, Aggressive Behavior and Social Acceptance in Early and Late Adolescence显示文摘 | Liisa Kehikangas-Ja '' rvinen | 2002 | Journal of Youth and Adolescence2002，31，27： | 1 |
| 17 | Effect of processing on the extractability of oat β-glucan显示文摘 | Liisa Johansson P?ivi Tuomainen Heli Anttila Hannu Rita Liisa Virkki | 2007 | Food Chemistry2007，，4： | 1 |
| 18 | The effect of phytic acid and some natural chelating agents on the solubility of mineral elements in oat bran显示文摘 | Paivi E Liisa V Maija Y | 2003 | Food Chemistry2003，80，： | 1 |
| 19 | Teenage experiences of public green spaces in suburban Helsinki显示文摘 | Kirsi M'akinena Liisa Tyrv'ainen | 2008 | Urban Forestry & Urban Greening2008，，7： | 1 |
| 20 | An evolutionary treasure:unification of a broad set of amidohydrolases related to urease显示文摘 | Liisa Holm Chris Sander | 1997 | Proteins1997，28，1： | 1 |
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