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| 1 | Single-nucleus RNA and ATAC sequencing reveals the impact of chromatin accessibility on gene expression in Arabidopsis roots at the singlecell level显示文摘Similar to other complex organisms,plants consist of diverse and specialized cell types.The gain of unique biological functions of these different cell types is the consequence of the establishment of cell-typespecific transcriptional programs.As a necessary step in gaining a deeper understanding of the regulatory mechanisms controlling plant gene expression,we report the use of single-nucleus RNA sequencing(sNucRNA-seq)and single-nucleus assay for transposase accessible chromatin sequencing(sNucATACseq)technologies on Arabidopsis roots.The comparison of our single-nucleus transcriptomes to the published protoplast transcriptomes validated the use of nuclei as biological entities to establish plant cell-type-specific transcriptomes.Furthermore,our sNucRNA-seq results uncovered the transcriptomes of additional cell subtypes not identified by single-cell RNA-seq.Similar to our transcriptomic approach,the sNucATAC-seq approach led to the distribution of the Arabidopsis nuclei into distinct clusters,suggesting the differential accessibility of chromatin between groups of cells according to their identity.To reveal the impact of chromatin accessibility on gene expression,we integrated sNucRNA-seq and sNucATAC-seq data and demonstrated that cell-type-specific marker genes display cell-type-specific patterns of chromatin accessibility.Our data suggest that the differential chromatin accessibility is a critical mechanism to regulate gene activity at the cell-type level. | Andrew Farmer Sandra Thibivilliers Kook Hui Ryu John Schiefelbein Marc Libault | 2021 | Molecular Plant2021，14，3： | 9 |
| 2 | Genetics andfunctional genomics of legume nodulation显示文摘 | Stacey G Libault M Brechenmacher L | 2006 | Current Opinion inPlant Biology2006，9，2： | 1 |
| 3 | Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic Bacterium Bradyrhizobiumjaponicum 显示文摘 | Brechenmacher L Lei Z Libault M | 2010 | Plant Physiology2010，153，4： | 1 |
| 4 | Genetics and functional genomics of legume nodulation显示文摘 | Stacey G Libault M Brechenmacher L | 2006 | Current Opinion in Plant Biology2006，9，2： | 1 |
| 5 | Identification of four so ybean reference genes for gene expression normalization 显示文摘 | Libault M Thibivilliers S Bilgin D D | 2008 | Plant Genome2008，7，： | 1 |
| 6 | Genetics and functional genomics of legume nodulation 显示文摘 | STACEY G LIBAULT M BRECHENMACHER L | 2006 | Current Opinion in Plant Biology2006，9，2： | 1 |
| 7 | An integrated transcriptome atlas of the crop model Glycine max and its use in comparative analyses in plants显示文摘 | Libault M Farmer A Joshi T Takahashi K Langley R.J Franklin L.D He J Xu D May G and Stacey G | | 0，，01： | 1 |
| 8 | Identification of four soybean reference genes for gene expression normalization显示文摘 | Libault M Thibivilliers S Bilgin D D | 2008 | The Plant Genome2008，，1： | 1 |
| 9 | The Arabidopsis LHP1 protein is a component of euchromatin显示文摘 | Libault M Tessadori F Germann S | 2005 | Planta2005，222，： | 1 |
| 10 | Identifi- cation of 118 Arabidopsis transcription factor and 30 ubiq- uitin-ligase genes responding to chitin, a plant-defense elicitor显示文摘 | LIBAULT M WAN J CZECHOWSKI T | 2007 | Molecular plant-microbe interactions : MPMI2007，20，8： | 1 |
| 11 | Identification of four soybean reference genes for gene expression normalization显示文摘 | Libault M Thibivilliers S Bilgin D | 2008 | Plant Genome2008，1，1： | 1 |
| 12 | The fate of duplicated genes in a polyploid plant genome 显示文摘 | Roulin A Auer PL Libault M Schlueter J Farmer A May G Stacey G Doerge RW Jackson SA | 2013 | Plant J2013，73，1： | 1 |
| 13 | Genetics and functional genomics of legume nodulation 显示文摘 | Staeey G Libault M Brechenmacher | 2006 | Current Opinion in Plant Biology2006，9，： | 1 |
| 14 | Identification of four soybean reference genes for gene expression normalization显示文摘 | Libault M Thibivilliers S Bilgin D D | 2008 | The Plant Genome2008，1，： | 1 |
| 15 | An integrated transcriptome atlas of the crop model Glycine max,and its use in comparative analyses in plants显示文摘 | Libault M Farmer A Joshi T | 2010 | The Plant Journal2010，63，1： | 1 |
| 16 | Identification of four soybean reference genes for gene expression normalization显示文摘 | LIBAULT M THIBIVILLIERS S BILGIN D D | 2008 | The Plant Genome2008，1，1： | 1 |
| 17 | Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin,a plant-defense elicitor显示文摘 | LIBAULT M WAN J CZECHOWSKI T | 2007 | Mol Plant Microbe Interact2007，20，： | 1 |
| 18 | The fate of duplicated genes in a polyploidy plant genome显示文摘 | Roulin A Auer P Libault M | | Plant Journal0，73，： | 1 |
| 19 | An integrated transcriptome atlas of the crop model Glycine max, and its use in comparative analyses in plants显示文摘 | Libault M Farmer A Joshi T | 2010 | Plant Journal2010，63，1： | 1 |
| 20 | An integrated transcriptome atlas of the crop model Glycine max, and its use in comparative analyses in plants 显示文摘 | Libault M Farmer A Joshi T | 2010 | The Plant Journal2010，63，1： | 1 |
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