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| 1 | Anticancer effect and apoptosis induction of gambogic acid in human gastric cancer line BGC-823显示文摘AIM: To investigate the anticancer effect of a traditional Chinese medicine gambogic acid (GA) in human gastric cancer line BGC-823 and further study the mechanism of apoptosis induction of GA.METHODS: Low differential human gastric cancer line BGC-823 were treated with GA at different doses and different times, the inhibitory rates were detected by MTT assay. Apoptosis induced by GA in BGC-823 cells was observed by Annexin-V/PI doubling staining flow cytometry assay. And T/C (%) was chosen to detect the inhibition of GA on human gastric adenocarcinoma BGC-823 nude mice xenografts. Apoptosis on nude mice xenografts was observed by Annexin-V/PI doubling staining flow cytometry assay and DNA fragmentation assay. To further determine the molecular mechanism of apoptosis induced by GA, the changes on the expression of bcl-2 and bax genes were detected by RT-PCR.RESULTS: After incubation with GA, low differential human gastric cancer line BGC-823 was dramatically inhibited in a dose-dependent manner. After these cells were exposedto GA for 24, 48 and 72 h, the IC50 value was 1.02±0.05, 1.41±0.20 and 1.14±0.19 μmol/L, respectively. Apoptosis in BGC-823 cells induced by GA was observed by AnnexinV/PI doubling staining flow cytometry assay. The apoptotic population of BGC-823 cells was about 12.96% and 24.58%, respectively, when cells were incubated with 1.2 μmol/L GA for 48 and 72 h. T/C (%) of human gastric carcinoma adenocarcinoma BGC-823 nude mice xenografts was 44.3, when the nude mice were treated with GA (8 mg/kg). Meanwhile, apoptosis induced by GA was observed in human gastric carcinoma adenocarcinoma BGC-823 nude mice xenografts. The increase of bax gene and the decrease of bc1-2 gene expressions were found by RT-PCR.CONCLUSION: The inhibition of GA on human gastric cancer line BGC-823 was confirmed. This effect connects with the inducing apoptosis in BGC-823 cells and the molecular mechanism might be related to the reduction of expression of apoptosis-regulated gene bcl-2, and the improvement of the expression of apoptosis-regulated gene bax. The result was also confirmed in vivo. | WeiLiu Qing-LongGuo Qi-DongYou LiZhao Hong-YanGu Sheng-TaoYuan | 2005 | World Journal of Gastroenterology2005，11，24： | 33 |
| 2 | General gambogic acids inhibited growth of human hepatoma SMMC-7721 cells in vitro and in nude mice显示文摘AIM: To study the inhibitory effect of general gambogic acids (GGA) on transplantation tumor SMMC-7721 in experimental animal model and SMMC-7721 cells in vitro. METHODS: Anti-tumor activity of GGA in the experi- mental transplantation tumor SMMC-7721 was evaluated by relative tumor growth ratio. Cell morphology was observed with inverted microscope and electron microscope. Cell proliferation was measured by MTT assay and the telomerase activity was determined by PCR. RESULTS: In vivo study indicated that GGA (2, 4, and 8 mg/kg, iv, 3 times per week for 3 weeks) displayed an inhibitory effect on the growth of transplantation tumor SMMC- 7721 in nude mice compared with the normal saline group (P<0.01). At the concentrations of 0.625-5.0 mg/L, GGA remarkably inhibited the proliferation of SMMC-7721 cells in vitro. GGA 2 mg/L dramatically changed morphology of SMMC-7721 cells and inhibited the telomerase activity in SMMC-7721 cells. CONCLUSION: GGA had inhibitory effect on the growth of SMMC-7721, which might be related to its inhibition of telomerase activity. | Qing-longGUO Qi-dongYOU Zhao-qiuWU Sheng-taoYUAN LiZHAO | 2004 | Acta Pharmacologica Sinica2004，25，6： | 31 |
| 3 | Expression of subtypes of somatostatin receptors in hepatic stellate cells显示文摘AIM: To elucidate the mechanism by which somatostatin and its analogue exert the influence on liver fibrosis, and to investigate the mRNA expression of somatostatin receptors subtypes (SSTRs) and the distribution of somatostatin analogue octreotide in rat hepatic stellate cells (HSCs). METHODS: HSCs were isolated from Sprague Dawley (SD) rats by in situ perfusion and density gradient centrifugation. After several passages, the mRNA expression of 5 subtypes of SSTRs were assessed by reverse transcription-polymerase chain reaction (RT-PCR). HSCs were planted on coverslip and co-cultured with octreotide tagged by FITC. Then the distribution of FITC fluorescence was observed under laser scanning confocal microscope (LSCM) in 12-24 h. RESULTS: There were mRNA expression of SSTR2, SSTR3 and SSTR5 but not SSTR1 and SSTR4 in SD rat HSCs. The mRNA expression level of SSTR2 was significantly higher than that of other subtypes (P<0.01). FITC fluorescence of octreotide was clearly observed on the surface and in the cytoplasm, but not in the nuclei of HSCs under LSCM. CONCLUSION: The effect exerted by somatostatin and its analogues on HSCs may mainly depend on the expression of SSTR2, SSTR3 and SSTR5. Octreotide can perfectlycombine with HSCs, and thereby exerts its biological activity on regulating the characters of active HSCs. This provides a potential prevention and management against liver fibrosis. | Sheng-HanSong Xi-ShengLeng TaoLi Zhi-ZhongQin Ji-RunPeng LiZhao Yu-HuaWei XinYu | 2004 | World Journal of Gastroenterology2004，10，11： | 8 |
| 4 | Preliminary evaluation of the long-term GLASS albedo product显示文摘Land surface albedo is an important parameter to describe the radiant forcing in the climate system.A long-time series of global albedo products is needed to understand the mechanism of climate change.Aiming to support global change and Earth system studies,GLASS(Global LAnd Surface Satellites)provides long-term global land surface albedo product from 1981 to 2010,which are generated from multisource remote sensing data and newly developed algorithms.It is critical to assess the quality of the GLASS product when it is released to the public.This paper first introduced the algorithms and then analyzed the integrity,accuracy,and robustness of the GLASS albedo product.The results show that the GLASS albedo product is a gapless,long-term continuous,and self-consistent data-set with an accuracy similar to that of the widely acknowledged MODIS MCD43 product.The quality flag,which is provided along with the black-sky and white-sky albedo,gives a pertinent indication of the expected uncertainty in the product. | Qiang Liua Lizhao Wang Ying Qu Nanfeng Liu Suhong Liu Hairong Tang Shunlin Liang | 2013 | International Journal of Digital Earth2013，6，S01： | 7 |
| 5 | Multi-scale validation strategy for satellite albedo products and its uncertainty analysis显示文摘Coarse-resolution satellite albedo products are important for climate change and energy balance research because of their capability to characterize the spatiotemporal patterns of land surface parameters at both the regional and global scales. The accuracy of coarse-resolution products is usually assessed via comparison with in situ measurements. The key issue in the comparison of remote sensing observations with in situ measurements is scaling and uncertainty. This paper presents a strategy for validating 1-km-resolution remote sensing albedo products using field measurements and high-resolution remote sensing observations. Field measurements were collected to calibrate the high-resolution(30 m) albedo products derived from HJ-1a/b images. Then, the calibrated high-resolution albedo maps were resampled(i.e., upscaled) to assess the accuracy of the coarse-resolution albedo products. The samples of field measurements and high-resolution pixels are based on an uncertainty analysis. Two types of coarse-resolution albedo datasets, from global land surface satellite(GLASS) and moderate-resolution imaging spectroradiometer(MODIS), are validated over the middle reaches of the Heihe River in China. The results indicate that the upscaled HJ(Huan Jing means environment in Chinese and this refers to a satellite constellation designed for environment and disaster monitoring by China) albedo, which was calibrated using field measurements, can provide accurate reference values for validating coarse-resolution satellite albedo products. However, the uncertainties in the upscaled HJ albedo should be estimated, and pixels with large uncertainties should be excluded from the validation process. | PENG JingJing LIU Qiang WEN JianGuang LIU QinHuo TANG Yong WANG LiZhao DOU BaoCheng YOU DongQin SUN ChangKui ZHAO XiaoJie FENG YouBin SHI Jian | 2015 | Science China Earth Sciences2015，58，4： | 5 |
| 6 | Expression of cancer-testis antigens in hepatocellular carcinoma显示文摘AIM: To investigate the expression of cancer-testis (CT) anigens MAGE-1, SSX-1 ,CTp11 and HCA587 genes in hepatocellular carcinoma (HCC) and the possibility of applying these antigens as targets for specific immunotherapy for HCC. METHODS: Expression levels of MAGE-1, SSX-1, CTp11 and HCA587mRNA were detected with reverse transcription polymerase chain reaction (RT-PCR) in HCC tissues and corresponding adjacent non-cancerous tissues from 105 HCC patients, 40 samples of cirrhosis and normal liver tissues.Genes of five samples with positive PCR results were sequenced.RESULTS: Of 105 HCC tissues, MAGE1, SSX-1 ,CTp11 and HCA587mRNA expressions were detectable in 75.2%(79/105),72.4%(76/105), 62.9%(66/105) and 56.2%(59/105) of HCC samples, respectively. About 93.3%(98/105), 72.4%(76/105),48.6%(51/105) and 37.1%(39/105) of HCC tissues positively expressed at least one, two, three, and four members of CT antigens, respectively. Conversely, only SSX-1 could be detectable in 2.9%(3/105) of the corresponding adjacent non-HCC tissues in which no metastatic lesion was found.Of the latter 3 patients, biopsy samples far from tumor were obtained in 2 patients and RT-PCR indicated no expression of SSX-1 mRNA in these two samples. In addition,none of 40 samples of cirrhotic and normal liver tissues expressed CT antigen gene mRNA. DNA sequences confirmed that the RT-PCR products were true target cDNA. No relationship was found between expression of CT antigens and clinico pathological indicators such as age,gender, tumor size, degree of tumor differentiation, serum α-fetoprotein level and infection of hepatitis B virus or hepatitis C virus (P>0.05).CONCLUSION: CT antigens genes (MAGE-1, SSX-1, CTp11 and HCA587) are expressed with high percentage and specificity in HCC and their products are promising targets for antigen-specific immunotherapy of HCC. High frequent co-expression of multiple members of CT antigens in HCC provides possibility of polyvalent vaccinations for HCC. | LiZhao Dong-ChengMou Xi-ShengLeng Ji-RunPeng Wan-XiangWang LeiHuang ShuLi Ji-YeZhu | 2004 | World Journal of Gastroenterology2004，10，14： | 4 |
| 7 | Anisotropic NdFeB/SmCoCuFeZr composite bonded magnet prepared by warm compaction process显示文摘Anisotropic NdFeB/SmCoCuFeZr composite bonded magnets were prepared by warm compaction process. The effects of adding SmCoCuFeZr magnetic powder on the properties of anisotropic bonded NdFeB magnet were investigated in this work. The results show that, both magnetic properties and temperature stability of the bonded magnet can be improved by adding fine SmCoCuFeZr magnetic powder. In the present study, the optimal content of SmCoCuFeZr magnetic powder was about 20 wt.%, in this case, the Br, Hcj, and(BH)maxof the NdFeB/SmCoCuFeZr composite magnet achieved 0.943 T, 1250 kA/m, and168 kJ/m^3, respectively. | Mengling Wu Yuping Li Xin Wang Lizhao Chen Yaozhao Mu | 2017 | Journal of Rare Earths2017，35，12： | 4 |
| 8 | Quantum conductance of armchair carbon nanocoils: roles of geometry effects显示文摘Armchair carbon nanocoils (CNCs) with different geometric parameters are constructed and optimized using a tight-binding (TB) total energy model. The quantum conductance of these nanocoils is simulated employing a π-orbital TB model incorporated with the non-equilibrium Green's function theory. Compared with the perfect armchair carbon nanotubes (CNTs) and armchair CNTs with only Stone-Wales (SW) defects, the quantum conductance spectra of the armchair CNCs present distinct gaps around the Fermi level, which are mainly originated from the existence of sp3 carbon in the three-dimensional spiral structures. Moreover, the detailed conductance spectra of the armchair CNCs depend sensitively on their geometric parameters, such as tubular diameter and block-block distance. | LIU LiZhao GAO HaiLi ZHAO JiJun LU JianPing | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，5： | 3 |
| 9 | SP1 governs primordial folliculogenesis by regulating pregranulosa cell development in mice显示文摘Establishment of the primordial follicle(PF)pool is pivotal for the female reproductive lifespan;however,the mechanism of primordial folliculogenesis is poorly understood.Here,the transcription factor SP1 was shown to be essential for PF formation in mice.Our results showed that SP1 is present in both oocytes and somatic cells during PF formation in the ovary.Knockdown of Sp1 expression,especially in pregranulosa cells,significantly suppressed nest breakdown,oocyte apoptosis,and PF formation,suggesting that SP1 expressed by somatic cells functions in the process of primordial folliculogenesis.We further demonstrated that SP1 governs the recruitment and maintenance of Forkhead box L2-positive(FOXL2^+)pregranulosa cells using an Lgr5-EGFP-IRES-CreER^T2(Lgr5-KI)reporter mouse model and a FOXL2^+ cell-specific knockdown model.At the molecular level,SP1 functioned mainly through manipulation of NOTCH2 expression by binding directly to the promoter of the Notch2 gene.Finally,consistent with the critical role of granulosa cells in follicle survival in vitro,massive loss of oocytes in SP1 knockdown ovaries was evidenced before puberty after the ovaries were tranSP1anted under the renal capsules.Conclusively,our results reveal that SP1 controls the establishment of the ovarian reserve by regulating pregranulosa cell development in the mammalian ovary. | Han Cai Bingying Liu Huarong Wang Guanghong Sun Lizhao Feng Ziqi Chen Jiaqi Zhou Jiawei Zhang Tuo Zhang Meina He Tingting Yang Qirui Guo Zhen Teng Qiliang Xin Bo Zhou Hua Zhang Guoliang Xia Chao Wang | 2020 | Journal of Molecular Cell Biology2020，12，3： | 3 |
| 10 | Grid Services Trusted Authentication Algorithm Based on Image Trusted Root in Scale Space显示文摘A new authentication algorithm for grid identity trusted computing unlimited by hardware is presented;the trusted root is made as an image data.The grid entity is trusted in the soft platform when its feature of image root is entirely matched with that from the other entities' feature database in a scale space process.To recognize and detect the stable image root feature,the non-homogeneous linear expandable scale space is proposed.Focusing on relations between the scale parameter of the inhomogeneous Gaussian function terms and the space evolution of thermal diffusion homogeneous equations,three space evolution operators are constructed to exact and mark the feature from image root.Analysis and verification are carried on the new scale space,operators and the core of making decisions for grid entities certifications. | LIU Lizhao LIU Jian ZHU Shunzhi LI Lin SUN Zhonghai | 2012 | Wuhan University Journal of Natural Sciences2012，17，6： | 2 |
| 11 | Th17 cells: The emerging reciprocal partner of regulatory T cells in the liver显示文摘 | LiZHAO De KaiQIU XiongMA | 2010 | Journal of Digestive Diseases2010，，3： | 2 |
| 12 | Design and Implementation of Verification Code Identification Based on Anisotropic Heat Kernel显示文摘Many websites use verification codes to prevent users from using the machine automatically to register,login,malicious vote or irrigate but it brought great burden to the enterprises involved in internet marketing as entering the verification code manually.Improving the verification code security system needs the identification method as the corresponding testing system.We propose an anisotropic heat kernel equation group which can generate a heat source scale space during the kernel evolution based on infinite heat source axiom,design a multi-step anisotropic verification code identification algorithm which includes core procedure of building anisotropic heat kernel,settingwave energy information parameters,combing outverification codccharacters and corresponding peripheral procedure of gray scaling,binarizing,denoising,normalizing,segmenting and identifying,give out the detail criterion and parameter set.Actual test show the anisotropic heat kernel identification algorithm can be used on many kinds of verification code including text characters,mathematical,Chinese,voice,3D,programming,video,advertising,it has a higher rate of 25%and 50%than neural network and context matching algorithm separately for Yahoo site,49%and 60%for Captcha site,20%and 52%for Baidu site,60%and 65%for 3DTakers site,40%,and 51%.for MDP site. | LIU Lizhao LIU Jian DAI Yaomei XU Huarong YIN Huayi ZHU Shunzhi | 2016 | China Communications2016，13，1： | 2 |
| 13 | miR-122-induced down-regulation of HO-1 negatively affects miR-122-mediated suppression of HBV显示文摘 | Lipeng Qiu Hongxia Fan Wensong Jin Bao Zhao Yanzhong Wang Ying Ju Lizhao Chen Yu Chen Zhongping Duan Songdong Meng | 2010 | Biochemical and Biophysical Research Communications2010，，4： | 2 |
| 14 | Forecasting the volatility of EUA futures with economic policy uncertainty using the GARCH‑MIDAS model显示文摘This study investigates the impact of economic policy uncertainty(EPU)on the volatility of European Union(EU)carbon futures prices and whether it has predictive power for the volatility of carbon futures prices.The GARCH-MIDAS model is applied for evaluating the impact of different EPU indexes on the price volatility of European Union Allowance(EUA)futures.We then compare the predictive power for the volatility of the two GARCH-MIDAS models based on different EPU indexes and six GARCH-type models.Our empirical results show that the GARCH-MIDAS models,which exhibit superior out-of-sample predictive ability,outperform GARCH-type models.The results also indicate that EPU has noticeable effect on the volatility of EUA futures.Specifically,the forecast accuracy of the EU EPU index is significantly higher than that of the global EPU index.Robustness checks further confirm that the EPU index(especially the EPU index of the EU)has strong predictive power for EUA futures prices.Additionally,using the volatility forecasting methods that GARCH-MIDAS models combine with the EPU index,investors can construct their portfolios to realize economic returns. | Jian Liu Ziting Zhang Lizhao Yan Fenghua Wen | 2021 | Financial Innovation2021，7，1： | 2 |
| 15 | Loss of microRNA 122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin G1‐modulated P53 activity显示文摘 | Saifeng Wang Lipeng Qiu Xiaoli Yan Wensong Jin Yanzhong Wang Lizhao Chen Erjie Wu Xin Ye George F. Gao Fusheng Wang Yu Chen Zhongping Duan Songdong Meng | 2012 | Hepatology2012，，3： | 1 |
| 16 | New fossil evidence and diet analysis of Gigantopithecus blacki and its distribution and extinction in South China显示文摘 | ZHAO Lingxia ZHANG Lizhao | 2013 | Quaternary International2013，286，： | 1 |
| 17 | Kindlin 2 promotes breast cancer invasion via epigenetic silencing of the microRNA200 gene family显示文摘 | Yu Yu Junzhou Wu Lizhao Guan Lihua Qi Yan Tang Bo Ma Jun Zhan Yunling Wang Weigang Fang Hongquan Zhang | 2013 | Int J Cancer2013，，6： | 1 |
| 18 | Kindlin-2 promotes genome instability in breast cancer cells显示文摘 | Ting Zhao Lizhao Guan Yu Yu Xuelian Pei Jun Zhan Ling Han Yan Tang Feng Li Weigang Fang Hongquan Zhang | 2012 | Cancer Letters2012，，： | 1 |
| 19 | Functional identification of gene cluster for the aniline metabolic pathway mediated by transposable element显示文摘A convenient and widely applicable method has been developed to clone aniline metabolic gene cluster in this study. Three positive recombinant plasmids pDA1, pDB2 and pDB11 were cloned from genomic library of aniline degradation strain AD9. The result of aniline dioxygenase (AD) activity and catechol 2,3-oxygenase (C23O) activity assay showed that pDA1 and pDB11 contain aniline dioxy-genase genes and catechol 2,3-dioxygenase genes, respectively. The sequence analysis of the total 24.7-kb region revealed that this region contains 25 ORFs, of which 17 genes involve metabolism of aniline. In the gene cluster, the first five genes (tadQTA1A2B) and the subsequent gene (tadR1) were pre-dicted to encode a multi-component aniline dioxygenase and a LysR-type regulator, respectively, while the others (tadD1C1D2C2EFGIJKL) were expected to encode meta- cleavage pathway enzymes for catechol degradation. The gene cluster was surrounded by two IS1071 sequences. | LIANG Quanfeng CHEN Ming xu Yuquan ZHANG Wei PING Shuzhen LU Wei SONG Xianlong WANG Weiwei GENG Lizhao Takeo Masahiro LIN Min | 2005 | Chinese Science Bulletin2005，50，15： | 1 |
| 20 | Clinical Study of the Role of 64-Slice CT Cerebra Angiography in Aneurysmal Subarachnoid Hemorrhage显示文摘 | Lizhao Chen Minhui Xu Yongwen Zou Lunshan Xu | 2014 | Cell Biochemistry and Biophysics2014，，3： | 1 |
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